Endodontic disease has mainly a microbial origin. It is caused by biofilms capable of attaching and surviving in the root canal. Therefore, it is important to study the conditions in which those biofilms grow, develop and colonize the root canal system. However, few studies have used natural teeth a...

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Autores:
Díez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia.
Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Echavarría González, Carolina
Serna Varona, Fátima
Rodríguez Ciodaro, Adriana
Tipo de recurso:
Article of journal
Fecha de publicación:
2017
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31303
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660
http://hdl.handle.net/10554/31303
Palabra clave:
Odontology; endodontics; microbiology
Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
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spelling Atribución-NoComercial-SinDerivadas 4.0 InternacionalCopyright (c) 2017 Universitas Scientiarumhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2This project was funded by the Center for Dental Research and its research line on Oral Pathologies and the Group of Infectious diseases and its research line Molecular and Applied Microbiology at Pontificia Universidad JaverianaDíez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia.Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.Echavarría González, CarolinaSerna Varona, FátimaRodríguez Ciodaro, Adriana2018-02-24T15:59:39Z2020-04-15T18:08:45Z2018-02-24T15:59:39Z2020-04-15T18:08:45Z2017-01-13http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1566010.11144/Javeriana.SC22-1.evmf2027-13520122-7483http://hdl.handle.net/10554/31303PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660/14489http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6904http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6906http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6907http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6908http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6957http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6958http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6959Universitas Scientiarum; Vol 22, No 1 (2017); 31-43Universitas Scientiarum; Vol 22, No 1 (2017); 31-43Universitas Scientiarum; Vol 22, No 1 (2017); 31-43Odontology; endodontics; microbiologyDental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.nullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleEx vivo model for studying polymicrobial biofilm formation in root canalsEndodontic disease has mainly a microbial origin. It is caused by biofilms capable of attaching and surviving in the root canal. Therefore, it is important to study the conditions in which those biofilms grow, develop and colonize the root canal system. However, few studies have used natural teeth as models, which would take into account the root canal anatomical complexity and simulate the clinical reality. In this study, we used human premolar root canals to standardize in vitro biofilm optimal formation conditions for microorganisms such as Enterococcus faecalis, Staphylococcus aureus and Candida albicans. 128 lower premolars underwent canal preparation using K-type files, and were treated with 5.25% sodium hypochlorite and EDTA. Samples were inoculated with microorganisms and incubated for 15, 30, 45, and 60 days under anaerobiosis (CO2 atmosphere) and aerobiosis. Microorganism presence was confirmed by Gram staining, cell culture, and electron microscopy. Exopolysaccharide matrix and microorganism aggregation were observed following 15 days of incubation. Bacterial growth towards the apical third of the root canal and biofilm maturation was detected after 30 days. CO2 atmosphere favored microbial growth the most. In vitro biofilm maturation was confirmed after 30 days of incubation under a CO2 atmosphere for both bacteria and yeast.10554/31303oai:repository.javeriana.edu.co:10554/313032023-03-28 16:15:57.34Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.english.eng.fl_str_mv Ex vivo model for studying polymicrobial biofilm formation in root canals
dc.creator.fl_str_mv Díez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia.
Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Echavarría González, Carolina
Serna Varona, Fátima
Rodríguez Ciodaro, Adriana
dc.contributor.author.none.fl_str_mv Díez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia.
Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Echavarría González, Carolina
Serna Varona, Fátima
Rodríguez Ciodaro, Adriana
dc.contributor.eng.fl_str_mv This project was funded by the Center for Dental Research and its research line on Oral Pathologies and the Group of Infectious diseases and its research line Molecular and Applied Microbiology at Pontificia Universidad Javeriana
dc.subject.eng.fl_str_mv Odontology; endodontics; microbiology
Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.
topic Odontology; endodontics; microbiology
Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.
spellingShingle Odontology; endodontics; microbiology
Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.
description Endodontic disease has mainly a microbial origin. It is caused by biofilms capable of attaching and surviving in the root canal. Therefore, it is important to study the conditions in which those biofilms grow, develop and colonize the root canal system. However, few studies have used natural teeth as models, which would take into account the root canal anatomical complexity and simulate the clinical reality. In this study, we used human premolar root canals to standardize in vitro biofilm optimal formation conditions for microorganisms such as Enterococcus faecalis, Staphylococcus aureus and Candida albicans. 128 lower premolars underwent canal preparation using K-type files, and were treated with 5.25% sodium hypochlorite and EDTA. Samples were inoculated with microorganisms and incubated for 15, 30, 45, and 60 days under anaerobiosis (CO2 atmosphere) and aerobiosis. Microorganism presence was confirmed by Gram staining, cell culture, and electron microscopy. Exopolysaccharide matrix and microorganism aggregation were observed following 15 days of incubation. Bacterial growth towards the apical third of the root canal and biofilm maturation was detected after 30 days. CO2 atmosphere favored microbial growth the most. In vitro biofilm maturation was confirmed after 30 days of incubation under a CO2 atmosphere for both bacteria and yeast.
publishDate 2017
dc.date.created.none.fl_str_mv 2017-01-13
dc.date.accessioned.none.fl_str_mv 2018-02-24T15:59:39Z
2020-04-15T18:08:45Z
dc.date.available.none.fl_str_mv 2018-02-24T15:59:39Z
2020-04-15T18:08:45Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660
10.11144/Javeriana.SC22-1.evmf
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/31303
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660
http://hdl.handle.net/10554/31303
identifier_str_mv 10.11144/Javeriana.SC22-1.evmf
2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660/14489
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http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6908
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6957
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6958
http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6959
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 22, No 1 (2017); 31-43
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 22, No 1 (2017); 31-43
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 22, No 1 (2017); 31-43
dc.rights.eng.fl_str_mv Copyright (c) 2017 Universitas Scientiarum
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.uri.eng.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
Copyright (c) 2017 Universitas Scientiarum
http://creativecommons.org/licenses/by-nc/4.0
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
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