Endodontic disease has mainly a microbial origin. It is caused by biofilms capable of attaching and surviving in the root canal. Therefore, it is important to study the conditions in which those biofilms grow, develop and colonize the root canal system. However, few studies have used natural teeth a...
- Autores:
-
Díez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia.
Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.
Echavarría González, Carolina
Serna Varona, Fátima
Rodríguez Ciodaro, Adriana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2017
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31303
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660
http://hdl.handle.net/10554/31303
- Palabra clave:
- Odontology; endodontics; microbiology
Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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Atribución-NoComercial-SinDerivadas 4.0 InternacionalCopyright (c) 2017 Universitas Scientiarumhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2This project was funded by the Center for Dental Research and its research line on Oral Pathologies and the Group of Infectious diseases and its research line Molecular and Applied Microbiology at Pontificia Universidad JaverianaDíez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia.Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia.Echavarría González, CarolinaSerna Varona, FátimaRodríguez Ciodaro, Adriana2018-02-24T15:59:39Z2020-04-15T18:08:45Z2018-02-24T15:59:39Z2020-04-15T18:08:45Z2017-01-13http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1566010.11144/Javeriana.SC22-1.evmf2027-13520122-7483http://hdl.handle.net/10554/31303PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660/14489http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6904http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6906http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6907http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6908http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6957http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6958http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6959Universitas Scientiarum; Vol 22, No 1 (2017); 31-43Universitas Scientiarum; Vol 22, No 1 (2017); 31-43Universitas Scientiarum; Vol 22, No 1 (2017); 31-43Odontology; endodontics; microbiologyDental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans.nullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleEx vivo model for studying polymicrobial biofilm formation in root canalsEndodontic disease has mainly a microbial origin. It is caused by biofilms capable of attaching and surviving in the root canal. Therefore, it is important to study the conditions in which those biofilms grow, develop and colonize the root canal system. However, few studies have used natural teeth as models, which would take into account the root canal anatomical complexity and simulate the clinical reality. In this study, we used human premolar root canals to standardize in vitro biofilm optimal formation conditions for microorganisms such as Enterococcus faecalis, Staphylococcus aureus and Candida albicans. 128 lower premolars underwent canal preparation using K-type files, and were treated with 5.25% sodium hypochlorite and EDTA. Samples were inoculated with microorganisms and incubated for 15, 30, 45, and 60 days under anaerobiosis (CO2 atmosphere) and aerobiosis. Microorganism presence was confirmed by Gram staining, cell culture, and electron microscopy. Exopolysaccharide matrix and microorganism aggregation were observed following 15 days of incubation. Bacterial growth towards the apical third of the root canal and biofilm maturation was detected after 30 days. CO2 atmosphere favored microbial growth the most. In vitro biofilm maturation was confirmed after 30 days of incubation under a CO2 atmosphere for both bacteria and yeast.10554/31303oai:repository.javeriana.edu.co:10554/313032023-03-28 16:15:57.34Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co |
dc.title.english.eng.fl_str_mv |
Ex vivo model for studying polymicrobial biofilm formation in root canals |
dc.creator.fl_str_mv |
Díez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia. Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia. Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia. Echavarría González, Carolina Serna Varona, Fátima Rodríguez Ciodaro, Adriana |
dc.contributor.author.none.fl_str_mv |
Díez Ortega, Hugo; Infectious Diseases Group, School of Sciences, Department of Microbiology, Pontificia Universidad Javeriana. Bogotá, Colombia. Correa Toral, Francisco; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia. Delgado Hernández, Leylin; Center for Dental Research, School of Dentistry, Pontificia Universidad Javeriana. Bogotá, Colombia. Echavarría González, Carolina Serna Varona, Fátima Rodríguez Ciodaro, Adriana |
dc.contributor.eng.fl_str_mv |
This project was funded by the Center for Dental Research and its research line on Oral Pathologies and the Group of Infectious diseases and its research line Molecular and Applied Microbiology at Pontificia Universidad Javeriana |
dc.subject.eng.fl_str_mv |
Odontology; endodontics; microbiology Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans. |
topic |
Odontology; endodontics; microbiology Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans. |
spellingShingle |
Odontology; endodontics; microbiology Dental Pulp Diseases, Biofilm, microbial interactions, Enterococcus faecalis, Staphylococcus aureus, Candida albicans. |
description |
Endodontic disease has mainly a microbial origin. It is caused by biofilms capable of attaching and surviving in the root canal. Therefore, it is important to study the conditions in which those biofilms grow, develop and colonize the root canal system. However, few studies have used natural teeth as models, which would take into account the root canal anatomical complexity and simulate the clinical reality. In this study, we used human premolar root canals to standardize in vitro biofilm optimal formation conditions for microorganisms such as Enterococcus faecalis, Staphylococcus aureus and Candida albicans. 128 lower premolars underwent canal preparation using K-type files, and were treated with 5.25% sodium hypochlorite and EDTA. Samples were inoculated with microorganisms and incubated for 15, 30, 45, and 60 days under anaerobiosis (CO2 atmosphere) and aerobiosis. Microorganism presence was confirmed by Gram staining, cell culture, and electron microscopy. Exopolysaccharide matrix and microorganism aggregation were observed following 15 days of incubation. Bacterial growth towards the apical third of the root canal and biofilm maturation was detected after 30 days. CO2 atmosphere favored microbial growth the most. In vitro biofilm maturation was confirmed after 30 days of incubation under a CO2 atmosphere for both bacteria and yeast. |
publishDate |
2017 |
dc.date.created.none.fl_str_mv |
2017-01-13 |
dc.date.accessioned.none.fl_str_mv |
2018-02-24T15:59:39Z 2020-04-15T18:08:45Z |
dc.date.available.none.fl_str_mv |
2018-02-24T15:59:39Z 2020-04-15T18:08:45Z |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.hasversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.local.spa.fl_str_mv |
Artículo de revista |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.driver.none.fl_str_mv |
info:eu-repo/semantics/article |
format |
http://purl.org/coar/resource_type/c_6501 |
dc.identifier.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660 10.11144/Javeriana.SC22-1.evmf |
dc.identifier.issn.none.fl_str_mv |
2027-1352 0122-7483 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/31303 |
url |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660 http://hdl.handle.net/10554/31303 |
identifier_str_mv |
10.11144/Javeriana.SC22-1.evmf 2027-1352 0122-7483 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.uri.none.fl_str_mv |
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/15660/14489 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6904 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6906 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6907 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6908 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6957 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6958 http://revistas.javeriana.edu.co/index.php/scientarium/article/downloadSuppFile/15660/6959 |
dc.relation.citationissue.eng.fl_str_mv |
Universitas Scientiarum; Vol 22, No 1 (2017); 31-43 |
dc.relation.citationissue.spa.fl_str_mv |
Universitas Scientiarum; Vol 22, No 1 (2017); 31-43 |
dc.relation.citationissue.por.fl_str_mv |
Universitas Scientiarum; Vol 22, No 1 (2017); 31-43 |
dc.rights.eng.fl_str_mv |
Copyright (c) 2017 Universitas Scientiarum |
dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional |
dc.rights.uri.eng.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
dc.rights.accessrights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional Copyright (c) 2017 Universitas Scientiarum http://creativecommons.org/licenses/by-nc/4.0 http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.spa.fl_str_mv |
PDF |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
null null null |
dc.publisher.eng.fl_str_mv |
Pontificia Universidad Javeriana |
institution |
Pontificia Universidad Javeriana |
repository.name.fl_str_mv |
Repositorio Institucional - Pontificia Universidad Javeriana |
repository.mail.fl_str_mv |
repositorio@javeriana.edu.co |
_version_ |
1811671379691438080 |