OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)

Conditions jor obtaining adventitious buds jrom shoot tips oj Podocarpus guatemalensis (Standley) cultured in Vitro were stablished. The explants were sur:face sterilized by inmersión in 2.5 per 100 sodium hypochlorite jor jour minutes. The jormation of adventitious buds was stimulated _soaking the...

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Autores:
Buitrago B., Esperanza; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
H. de Jaramillo, Elizabeth; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Tipo de recurso:
Article of journal
Fecha de publicación:
2013
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31945
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5085
http://hdl.handle.net/10554/31945
Palabra clave:
null
Micropropagación; Podocarpaceae
null
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
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network_acronym_str JAVERIANA2
network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullBuitrago B., Esperanza; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, BogotáH. de Jaramillo, Elizabeth; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá2018-02-24T16:01:01Z2020-04-15T18:08:46Z2018-02-24T16:01:01Z2020-04-15T18:08:46Z2013-05-17http://revistas.javeriana.edu.co/index.php/scientarium/article/view/50852027-13520122-7483http://hdl.handle.net/10554/31945Conditions jor obtaining adventitious buds jrom shoot tips oj Podocarpus guatemalensis (Standley) cultured in Vitro were stablished. The explants were sur:face sterilized by inmersión in 2.5 per 100 sodium hypochlorite jor jour minutes. The jormation of adventitious buds was stimulated _soaking the shoot tips in 100 mg!L EDTA solution jor 20 minutes befare transjering them on aANA (0.0-0.02rng!L) andBA (2.5 -5.0rng!L) supplemented Gressho.ff and Doy (G.D.) liquid medium The buds were cultured under red light and a photoperiod oj 16 h at 24°C.Ajter 30 days oj culture, the buds were tranjerred to G.D. medium diluted 1:1, without hormones and supplemented with 5 per 100 glicine, 3 per 1 00 sucrose and 1 per 100 actived charcoal to stimulate the development adventüious bud primordia. Each explant jormed 1 - 1 O adventitious buds.PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/5085/3925Universitas Scientiarum; Vol 1, No 3 (1988); 13-25Universitas Scientiarum; Vol 1, No 3 (1988); 13-25Universitas Scientiarum; Vol 1, No 3 (1988); 13-25nullMicropropagación; PodocarpaceaenullOBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)nullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/article10554/31945oai:repository.javeriana.edu.co:10554/319452023-03-28 16:15:27.013Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.spa.fl_str_mv OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
title OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
spellingShingle OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
null
Micropropagación; Podocarpaceae
null
title_short OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
title_full OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
title_fullStr OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
title_full_unstemmed OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
title_sort OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)
dc.creator.fl_str_mv Buitrago B., Esperanza; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
H. de Jaramillo, Elizabeth; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.author.none.fl_str_mv Buitrago B., Esperanza; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
H. de Jaramillo, Elizabeth; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.none.fl_str_mv null
dc.subject.spa.fl_str_mv null
Micropropagación; Podocarpaceae
null
topic null
Micropropagación; Podocarpaceae
null
description Conditions jor obtaining adventitious buds jrom shoot tips oj Podocarpus guatemalensis (Standley) cultured in Vitro were stablished. The explants were sur:face sterilized by inmersión in 2.5 per 100 sodium hypochlorite jor jour minutes. The jormation of adventitious buds was stimulated _soaking the shoot tips in 100 mg!L EDTA solution jor 20 minutes befare transjering them on aANA (0.0-0.02rng!L) andBA (2.5 -5.0rng!L) supplemented Gressho.ff and Doy (G.D.) liquid medium The buds were cultured under red light and a photoperiod oj 16 h at 24°C.Ajter 30 days oj culture, the buds were tranjerred to G.D. medium diluted 1:1, without hormones and supplemented with 5 per 100 glicine, 3 per 1 00 sucrose and 1 per 100 actived charcoal to stimulate the development adventüious bud primordia. Each explant jormed 1 - 1 O adventitious buds.
publishDate 2013
dc.date.created.none.fl_str_mv 2013-05-17
dc.date.accessioned.none.fl_str_mv 2018-02-24T16:01:01Z
2020-04-15T18:08:46Z
dc.date.available.none.fl_str_mv 2018-02-24T16:01:01Z
2020-04-15T18:08:46Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5085
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/31945
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5085
http://hdl.handle.net/10554/31945
identifier_str_mv 2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5085/3925
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 1, No 3 (1988); 13-25
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 1, No 3 (1988); 13-25
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 1, No 3 (1988); 13-25
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
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