OBTENCION IN VITRO DE YEMAS ADVENTICIAS DE Podocarpus guatemalensis (STANDLEY)

Conditions jor obtaining adventitious buds jrom shoot tips oj Podocarpus guatemalensis (Standley) cultured in Vitro were stablished. The explants were sur:face sterilized by inmersión in 2.5 per 100 sodium hypochlorite jor jour minutes. The jormation of adventitious buds was stimulated _soaking the shoot tips in 100 mg!L EDTA solution jor 20 minutes befare transjering them on aANA (0.0-0.02rng!L) andBA (2.5 -5.0rng!L) supplemented Gressho.ff and Doy (G.D.) liquid medium The buds were cultured under red light and a photoperiod oj 16 h at 24°C.Ajter 30 days oj culture, the buds were tranjerred to G.D. medium diluted 1:1, without hormones and supplemented with 5 per 100 glicine, 3 per 1 00 sucrose and 1 per 100 actived charcoal to stimulate the development adventüious bud primordia. Each explant jormed 1 - 1 O adventitious buds.

• Autores: Buitrago B., Esperanza; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá; H. de Jaramillo, Elizabeth; Unidad de Biología Vegetal, Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
• Tipo de recurso: Article of journal
• Fecha de publicación: 2013
• Institución: Pontificia Universidad Javeriana
• Repositorio: Repositorio Universidad Javeriana
• Idioma: eng
• OAI Identifier: oai:repository.javeriana.edu.co:10554/31945
• Acceso en línea: http://revistas.javeriana.edu.co/index.php/scientarium/article/view/5085; http://hdl.handle.net/10554/31945
• Palabra clave: null; Micropropagación; Podocarpaceae; null
• Rights: openAccess
• License: Atribución-NoComercial-SinDerivadas 4.0 Internacional