INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES

The influence of sodium glutamate as a supplement to Latham Mueller medium, while using bubbling nitrogen flow as an anaerobic agent and superficial aeration as an inducer of cell lysis and as a mechanism for the haulage of gases in the fermentation processes was evaluated. Using the Clostridium tet...

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Autores:
Gutiérrez, I.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Garzón, E.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Vargas, P.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Moreno, N.; Grupo de Fermentaciones, Instituto de Biotecnología, Universidad Nacional de Colombia.
Poutou Piñales, Raúl Alberto; Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
Tipo de recurso:
Article of journal
Fecha de publicación:
2005
Institución:
Pontificia Universidad Javeriana
Repositorio:
Repositorio Universidad Javeriana
Idioma:
eng
OAI Identifier:
oai:repository.javeriana.edu.co:10554/31132
Acceso en línea:
http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984
http://hdl.handle.net/10554/31132
Palabra clave:
null
tetanus toxin; fermentation; N2; sodium glutamate
null
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
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oai_identifier_str oai:repository.javeriana.edu.co:10554/31132
network_acronym_str JAVERIANA2
network_name_str Repositorio Universidad Javeriana
repository_id_str
spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2nullGutiérrez, I.; Laboratorio de Tétanos, Instituto Nacional de Salud.Garzón, E.; Laboratorio de Tétanos, Instituto Nacional de Salud.Vargas, P.; Laboratorio de Tétanos, Instituto Nacional de Salud.Moreno, N.; Grupo de Fermentaciones, Instituto de Biotecnología, Universidad Nacional de Colombia.Poutou Piñales, Raúl Alberto; Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá2018-02-24T15:59:17Z2020-04-15T18:10:07Z2018-02-24T15:59:17Z2020-04-15T18:10:07Z2005-07-10http://revistas.javeriana.edu.co/index.php/scientarium/article/view/49842027-13520122-7483http://hdl.handle.net/10554/31132The influence of sodium glutamate as a supplement to Latham Mueller medium, while using bubbling nitrogen flow as an anaerobic agent and superficial aeration as an inducer of cell lysis and as a mechanism for the haulage of gases in the fermentation processes was evaluated. Using the Clostridium tetani Massachusetts’s strain, several five (5) liter batch fermentations were carried out for tetanus toxin production under the following conditions: Latham Mueller medium, with or without sodium glutamate, nitrogen flow and superficial aeration. The results demonstrated that the addition of sodium glutamate (2.5 g/l), combined with a bubbling nitrogen flow (0.33 l/min) and superficial aeration (0.33 l/min), produced asignificant increase in cell concentrations, repressing the tetanus toxin formation; while the gas flow (nitrogen and superficial aeration) without sodium glutamate improved the toxin production by approximately 49%, providing conditions for the following outcomes: a maximum toxin level of 73 Lf/ml; a toxin formation rate of 1844.0 Lf/l.h; and, an over-all productivity of 833.5 Lf/l.h.PDFapplication/pdfengPontificia Universidad Javerianahttp://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984/3840Universitas Scientiarum; Vol 10, No 2 (2005); 79-86Universitas Scientiarum; Vol 10, No 2 (2005); 79-86Universitas Scientiarum; Vol 10, No 2 (2005); 79-86nulltetanus toxin; fermentation; N2; sodium glutamatenullINFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURESnullnullnullhttp://purl.org/coar/version/c_970fb48d4fbd8a85Artículo de revistahttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/article10554/31132oai:repository.javeriana.edu.co:10554/311322023-03-28 16:15:35.695Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.co
dc.title.spa.fl_str_mv INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
title INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
spellingShingle INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
null
tetanus toxin; fermentation; N2; sodium glutamate
null
title_short INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
title_full INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
title_fullStr INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
title_full_unstemmed INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
title_sort INFLUENCE OF SODIUM GLUTAMATE, BUBBLING N2- GAS AND SUPERFICIAL AERATION ON TETANUS TOXIN PRODUCTION IN Clostridium tetani CULTURES
dc.creator.fl_str_mv Gutiérrez, I.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Garzón, E.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Vargas, P.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Moreno, N.; Grupo de Fermentaciones, Instituto de Biotecnología, Universidad Nacional de Colombia.
Poutou Piñales, Raúl Alberto; Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.author.none.fl_str_mv Gutiérrez, I.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Garzón, E.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Vargas, P.; Laboratorio de Tétanos, Instituto Nacional de Salud.
Moreno, N.; Grupo de Fermentaciones, Instituto de Biotecnología, Universidad Nacional de Colombia.
Poutou Piñales, Raúl Alberto; Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá
dc.contributor.none.fl_str_mv null
dc.subject.spa.fl_str_mv null
tetanus toxin; fermentation; N2; sodium glutamate
null
topic null
tetanus toxin; fermentation; N2; sodium glutamate
null
description The influence of sodium glutamate as a supplement to Latham Mueller medium, while using bubbling nitrogen flow as an anaerobic agent and superficial aeration as an inducer of cell lysis and as a mechanism for the haulage of gases in the fermentation processes was evaluated. Using the Clostridium tetani Massachusetts’s strain, several five (5) liter batch fermentations were carried out for tetanus toxin production under the following conditions: Latham Mueller medium, with or without sodium glutamate, nitrogen flow and superficial aeration. The results demonstrated that the addition of sodium glutamate (2.5 g/l), combined with a bubbling nitrogen flow (0.33 l/min) and superficial aeration (0.33 l/min), produced asignificant increase in cell concentrations, repressing the tetanus toxin formation; while the gas flow (nitrogen and superficial aeration) without sodium glutamate improved the toxin production by approximately 49%, providing conditions for the following outcomes: a maximum toxin level of 73 Lf/ml; a toxin formation rate of 1844.0 Lf/l.h; and, an over-all productivity of 833.5 Lf/l.h.
publishDate 2005
dc.date.created.none.fl_str_mv 2005-07-10
dc.date.accessioned.none.fl_str_mv 2018-02-24T15:59:17Z
2020-04-15T18:10:07Z
dc.date.available.none.fl_str_mv 2018-02-24T15:59:17Z
2020-04-15T18:10:07Z
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.hasversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.local.spa.fl_str_mv Artículo de revista
dc.type.coar.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/article
format http://purl.org/coar/resource_type/c_6501
dc.identifier.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984
dc.identifier.issn.none.fl_str_mv 2027-1352
0122-7483
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10554/31132
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984
http://hdl.handle.net/10554/31132
identifier_str_mv 2027-1352
0122-7483
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.uri.none.fl_str_mv http://revistas.javeriana.edu.co/index.php/scientarium/article/view/4984/3840
dc.relation.citationissue.eng.fl_str_mv Universitas Scientiarum; Vol 10, No 2 (2005); 79-86
dc.relation.citationissue.spa.fl_str_mv Universitas Scientiarum; Vol 10, No 2 (2005); 79-86
dc.relation.citationissue.por.fl_str_mv Universitas Scientiarum; Vol 10, No 2 (2005); 79-86
dc.rights.licence.*.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.accessrights.none.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.spa.fl_str_mv PDF
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv null
null
null
dc.publisher.eng.fl_str_mv Pontificia Universidad Javeriana
institution Pontificia Universidad Javeriana
repository.name.fl_str_mv Repositorio Institucional - Pontificia Universidad Javeriana
repository.mail.fl_str_mv repositorio@javeriana.edu.co
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