Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes
Q1
- Autores:
-
Bernal Forigua, Camila
Camero Bautista, Carlos Andres
Pinzón García, María José
Rodríguez, Sergio
Canas, Alejandra
Rojas, Adriana
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2023
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/65324
- Acceso en línea:
- https://www.sciencedirect.com/science/article/pii/S0147651323004049
http://hdl.handle.net/10554/65324
https://doi.org/10.1016/j.ecoenv.2023.114900
- Palabra clave:
- Electronic cigarettes
Vaping
Micronuclei
Genotoxicity
DNA methylation
LINE-1
- Rights
- License
- Atribución-NoComercial 4.0 Internacional
| id |
JAVERIANA2_09536090a81c48a96cac63a2cf52fecd |
|---|---|
| oai_identifier_str |
oai:repository.javeriana.edu.co:10554/65324 |
| network_acronym_str |
JAVERIANA2 |
| network_name_str |
Repositorio Universidad Javeriana |
| repository_id_str |
|
| dc.title.spa.fl_str_mv |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| title |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| spellingShingle |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes Electronic cigarettes Vaping Micronuclei Genotoxicity DNA methylation LINE-1 |
| title_short |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| title_full |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| title_fullStr |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| title_full_unstemmed |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| title_sort |
Genotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes |
| dc.creator.fl_str_mv |
Bernal Forigua, Camila Camero Bautista, Carlos Andres Pinzón García, María José Rodríguez, Sergio Canas, Alejandra Rojas, Adriana |
| dc.contributor.author.none.fl_str_mv |
Bernal Forigua, Camila Camero Bautista, Carlos Andres Pinzón García, María José Rodríguez, Sergio Canas, Alejandra Rojas, Adriana |
| dc.contributor.corporatename.spa.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. Neumología |
| dc.contributor.corporatename.none.fl_str_mv |
Pontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética Humana |
| dc.contributor.javerianateacher.none.fl_str_mv |
Canas, Alejandra Rojas, Adriana |
| dc.subject.keyword.spa.fl_str_mv |
Electronic cigarettes Vaping Micronuclei Genotoxicity DNA methylation LINE-1 |
| topic |
Electronic cigarettes Vaping Micronuclei Genotoxicity DNA methylation LINE-1 |
| description |
Q1 |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-08-28T16:04:40Z |
| dc.date.available.none.fl_str_mv |
2023-08-28T16:04:40Z |
| dc.date.created.none.fl_str_mv |
2023-04-11 |
| dc.type.local.spa.fl_str_mv |
Artículo de revista |
| dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
| format |
http://purl.org/coar/resource_type/c_6501 |
| dc.identifier.spa.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S0147651323004049 |
| dc.identifier.issn.spa.fl_str_mv |
0147-6513 / 1090-2414 (Electrónico) |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10554/65324 |
| dc.identifier.doi.spa.fl_str_mv |
https://doi.org/10.1016/j.ecoenv.2023.114900 |
| dc.identifier.instname.spa.fl_str_mv |
instname:Pontificia Universidad Javeriana |
| dc.identifier.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional - Pontificia Universidad Javeriana |
| dc.identifier.repourl.spa.fl_str_mv |
repourl:https://repository.javeriana.edu.co |
| url |
https://www.sciencedirect.com/science/article/pii/S0147651323004049 http://hdl.handle.net/10554/65324 https://doi.org/10.1016/j.ecoenv.2023.114900 |
| identifier_str_mv |
0147-6513 / 1090-2414 (Electrónico) instname:Pontificia Universidad Javeriana reponame:Repositorio Institucional - Pontificia Universidad Javeriana repourl:https://repository.javeriana.edu.co |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.citationstartpage.spa.fl_str_mv |
1 |
| dc.relation.citationendpage.spa.fl_str_mv |
9 |
| dc.relation.ispartofjournal.spa.fl_str_mv |
Ecotoxicology and Environmental Safety |
| dc.relation.citationvolume.spa.fl_str_mv |
256 |
| dc.rights.licence.*.fl_str_mv |
Atribución-NoComercial 4.0 Internacional |
| dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.coar.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
| rights_invalid_str_mv |
Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
| dc.format.spa.fl_str_mv |
PDF |
| dc.format.mimetype.spa.fl_str_mv |
application/pdf |
| dc.coverage.spatial.spa.fl_str_mv |
Colombia |
| dc.coverage.city.spa.fl_str_mv |
Bogotá (Colombia) |
| institution |
Pontificia Universidad Javeriana |
| bitstream.url.fl_str_mv |
http://repository.javeriana.edu.co/bitstream/10554/65324/1/Genotoxicity%20and%20hypomethylation%20of%20LINE-1%20induced%20by%20electronic%20cigarettes.pdf http://repository.javeriana.edu.co/bitstream/10554/65324/2/license.txt http://repository.javeriana.edu.co/bitstream/10554/65324/3/Genotoxicity%20and%20hypomethylation%20of%20LINE-1%20induced%20by%20electronic%20cigarettes.pdf.jpg |
| bitstream.checksum.fl_str_mv |
26ba3afe91ece5a10999c735ce2717f6 2070d280cc89439d983d9eee1b17df53 686e5aa7b7abafdab4bb96cbc3b50f14 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositorio Institucional - Pontificia Universidad Javeriana |
| repository.mail.fl_str_mv |
repositorio@javeriana.edu.co |
| _version_ |
1811671306784997376 |
| spelling |
Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/http://purl.org/coar/access_right/c_abf2Bernal Forigua, CamilaCamero Bautista, Carlos AndresPinzón García, María JoséRodríguez, SergioCanas, AlejandraRojas, AdrianaPontificia Universidad Javeriana. Facultad de Medicina. Departamento de Medicina Interna. NeumologíaPontificia Universidad Javeriana. Facultad de Medicina. Instituto de Genética Humana. Grupo de investigación Instituto de Genética HumanaCanas, AlejandraRojas, AdrianaColombiaBogotá (Colombia)2023-08-28T16:04:40Z2023-08-28T16:04:40Z2023-04-11https://www.sciencedirect.com/science/article/pii/S01476513230040490147-6513 / 1090-2414 (Electrónico)http://hdl.handle.net/10554/65324https://doi.org/10.1016/j.ecoenv.2023.114900instname:Pontificia Universidad Javerianareponame:Repositorio Institucional - Pontificia Universidad Javerianarepourl:https://repository.javeriana.edu.coPDFapplication/pdfengGenotoxicity and hypomethylation of LINE-1 induced by electronic cigarettesArtículo de revistahttp://purl.org/coar/resource_type/c_6501Q1Q1Individuos vapeadoresCurrently, the marketing of electronic cigarettes as a safe alternative to smoking has increased, which is associated with greater use of these devices, especially among young people and smokers interested in quitting tobacco cigarettes. Given the growing use of this type of product, there is a need to determine the consequences of electronic cigarettes on human health, especially since many of the compounds contained in the aerosol and liquid of these devices have a high potential to be carcinogenic and genotoxic. Additionally, many of these compounds' aerosol concentrations exceed the safe limits. We have evaluated the levels of genotoxicity and changes in DNA methylation patterns associated with vaping. We analyzed a total of 90 peripheral blood samples from a population of vapers (n = 32), smokers (n = 18), and controls (n = 32), in which the frequencies of genotoxicity were determined by the cytokinesis-blocking micronuclei (CBMN) assay and the patterns of methylation of the repetitive elements of LINE-1 through the Quantitative Methylation Specific PCR (qMSP) assay. Here we show an increase in genotoxicity levels associated with vaping habits. Additionally, the group of vapers showed changes at the epigenetic level specifically associated with the loss of methylation of the LINE-1 elements. These changes in LINE-1 methylation patterns were reflected in its representative RNA expression detected in vapers.https://orcid.org/0000-0003-1555-6661https://orcid.org/0000-0001-5650-6471https://orcid.org/0000-0002-1051-6356https://orcid.org/0000-0003-3975-2835https://orcid.org/0000-0001-8528-4433Revista Internacional - IndexadaA1NoElectronic cigarettesVapingMicronucleiGenotoxicityDNA methylationLINE-119Ecotoxicology and Environmental Safety256ORIGINALGenotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes.pdfGenotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes.pdfArtículoapplication/pdf3719452http://repository.javeriana.edu.co/bitstream/10554/65324/1/Genotoxicity%20and%20hypomethylation%20of%20LINE-1%20induced%20by%20electronic%20cigarettes.pdf26ba3afe91ece5a10999c735ce2717f6MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-82603http://repository.javeriana.edu.co/bitstream/10554/65324/2/license.txt2070d280cc89439d983d9eee1b17df53MD52open accessTHUMBNAILGenotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes.pdf.jpgGenotoxicity and hypomethylation of LINE-1 induced by electronic cigarettes.pdf.jpgIM Thumbnailimage/jpeg10383http://repository.javeriana.edu.co/bitstream/10554/65324/3/Genotoxicity%20and%20hypomethylation%20of%20LINE-1%20induced%20by%20electronic%20cigarettes.pdf.jpg686e5aa7b7abafdab4bb96cbc3b50f14MD53open access10554/65324oai:repository.javeriana.edu.co:10554/653242024-01-10 20:43:49.051Repositorio Institucional - Pontificia Universidad Javerianarepositorio@javeriana.edu.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 |