To develop proposals in bacterial formulations applicable to the agricultural sector, a study of physicochemical and biological parameters of the polymeric materials is essential. Here, we evaluated the effects of eight polymers on the cellular viability of Rhizobium sp. G58 during a 2-month period....
- Autores:
-
Rivera, Diego; Researcher Laboratorio de Microbiología de Suelos, CORPOICA
Obando, Melissa; Researcher Laboratorio de Microbiología de Suelos, CORPOICA
Barbosa, Helber; Researcher Universidad Nacional de Colombia
Rojas Tapias, Daniel; Researcher Laboratorio de Microbiología de Suelos, CORPOICA
Bonilla Buitrago, Ruth; Researcher Laboratorio de Microbiología de Suelos, CORPOICA
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2014
- Institución:
- Pontificia Universidad Javeriana
- Repositorio:
- Repositorio Universidad Javeriana
- Idioma:
- eng
- OAI Identifier:
- oai:repository.javeriana.edu.co:10554/31295
- Acceso en línea:
- http://revistas.javeriana.edu.co/index.php/scientarium/article/view/7396
http://hdl.handle.net/10554/31295
- Palabra clave:
- Microbiology-Applied Biotechnology
Rhizobium; polymers; formulation; analysis multivariate.
Agricultural microbiology
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
| Summary: | To develop proposals in bacterial formulations applicable to the agricultural sector, a study of physicochemical and biological parameters of the polymeric materials is essential. Here, we evaluated the effects of eight polymers on the cellular viability of Rhizobium sp. G58 during a 2-month period. From these results, we selected the three polymers that yielded the best results in respect to the survival of the bacteria. An assay of the effect of the polymers on the symbiotic activity of Rhizobium-Cowpea and the agronomic parameters was conducted under greenhouse conditions, based on the principal component analysis. A positive effect was found in Rhizobium sp. G58 using the Tukey’s Test (p<0.05) with sodium alginate (0.5-1%) and hydroxypropyl methylcellulose-HPMC (0.125-0.5%), while a significant decrease was established in cellular viability using polyethylene glycol-PEG, carbomer-Carbopol 940, and polyvinyl alcohol-PVA. The multivariate analysis indicated that the application of the polymers (sodium alginate and hydroxypropyl methylcellulose) in 0.5% concentration did not have negative effects on the symbiotic fixation of nitrogen or the process of nodulation. In conclusion, our results suggest the effectiveness of these polymers and the possibility of using them as carriers of bacterial formulation without affecting physiological processes. |
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