Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury
Conditions that produce ischemia and reperfusion injury include orthopedic surgeries, vascular diseases and accidents in remote places in which use of a manual tourniquet is required. Tissues under such stress suffer the consequences of evidenced by changes in their normal microscopic organization t...
- Autores:
-
Rosero Salazar, Doris Haydee
Florez Elvira, Liliana Janeth
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad ICESI
- Repositorio:
- Repositorio ICESI
- Idioma:
- eng
- OAI Identifier:
- oai:repository.icesi.edu.co:10906/82015
- Acceso en línea:
- http://nebulosa.icesi.edu.co:2143/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=2AccfnLPvcf3xKNWqCE&page=1&doc=1
http://hdl.handle.net/10906/82015
- Palabra clave:
- Isquemia
Reperfusion
Enfermedades vasculares
Ciencias socio biomédicas
Medical sciences
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-nd/4.0/
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| dc.title.spa.fl_str_mv |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| title |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| spellingShingle |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury Isquemia Reperfusion Enfermedades vasculares Ciencias socio biomédicas Medical sciences |
| title_short |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| title_full |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| title_fullStr |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| title_full_unstemmed |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| title_sort |
Image analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injury |
| dc.creator.fl_str_mv |
Rosero Salazar, Doris Haydee Florez Elvira, Liliana Janeth |
| dc.contributor.author.spa.fl_str_mv |
Rosero Salazar, Doris Haydee Florez Elvira, Liliana Janeth |
| dc.subject.spa.fl_str_mv |
Isquemia Reperfusion Enfermedades vasculares Ciencias socio biomédicas Medical sciences |
| topic |
Isquemia Reperfusion Enfermedades vasculares Ciencias socio biomédicas Medical sciences |
| description |
Conditions that produce ischemia and reperfusion injury include orthopedic surgeries, vascular diseases and accidents in remote places in which use of a manual tourniquet is required. Tissues under such stress suffer the consequences of evidenced by changes in their normal microscopic organization that can be reversible or irreversible according to the time and severity of lesion. An experimental model of ischemia has been designed taking into account the characteristics similar to a surgical procedure, from preparation for anesthesia up to the postsurgical follow up of each animal until it finishes the established time of reperfusion. Two muscles, soleus and extensor carpi radialis longus, dissected from Wistar rats that were underwent to short periods of ischemia and short and prolonged periods of reperfusion up to 32 days. There were no significant changes in the macroscopic weight of muscles, but significant differences were found in the area occupied by intramuscular extracellular matrix. During reperfusion, a partial recovery was observed until the last day of study. If we pretend to extrapolate these results to clinical areas, its importance focuses in the recovering of function and the following up of patients after surgical procedures as studied in the present experiment. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016-06-01 |
| dc.date.accessioned.none.fl_str_mv |
2017-09-08T00:14:24Z |
| dc.date.available.none.fl_str_mv |
2017-09-08T00:14:24Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Artículo |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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publishedVersion |
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2319-247X |
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http://nebulosa.icesi.edu.co:2143/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=2AccfnLPvcf3xKNWqCE&page=1&doc=1 |
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http://hdl.handle.net/10906/82015 |
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instname: Universidad Icesi |
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reponame: Biblioteca Digital |
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repourl: https://repository.icesi.edu.co/ |
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http://nebulosa.icesi.edu.co:2143/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=2AccfnLPvcf3xKNWqCE&page=1&doc=1 http://hdl.handle.net/10906/82015 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Exploratory Animal and Medical Research, Vol. 6, No.1 -2016 |
| dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.accessrights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.license.none.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) |
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http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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10 páginas |
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Digital |
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Hierarchy of Calcutta de Lat: 39 36 00 N degrees minutes Lat: 39.6000 decimal degrees Long: 087 04 00 W degrees minutes Long: -87.0667 decimal degrees |
| dc.publisher.spa.fl_str_mv |
West Bengal Veterinary ASSOC |
| dc.publisher.faculty.spa.fl_str_mv |
Facultad de Ciencias de la Salud |
| dc.publisher.program.spa.fl_str_mv |
Medicina |
| dc.publisher.department.spa.fl_str_mv |
Departamento de Ciencias Básicas Médicas |
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Calcutta |
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Universidad ICESI |
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http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/82015/3/rosero_image_analysis_2016.pdf http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/82015/2/license.txt |
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Biblioteca Digital - Universidad icesi |
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cdcriollo@icesi.edu.co |
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1808499125124071424 |
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Rosero Salazar, Doris HaydeeFlorez Elvira, Liliana Janethdorhaross@gmail.comHierarchy of Calcutta de Lat: 39 36 00 N degrees minutes Lat: 39.6000 decimal degrees Long: 087 04 00 W degrees minutes Long: -87.0667 decimal degrees2017-09-08T00:14:24Z2017-09-08T00:14:24Z2016-06-012319-247Xhttp://nebulosa.icesi.edu.co:2143/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=2AccfnLPvcf3xKNWqCE&page=1&doc=1http://hdl.handle.net/10906/82015instname: Universidad Icesireponame: Biblioteca Digitalrepourl: https://repository.icesi.edu.co/Conditions that produce ischemia and reperfusion injury include orthopedic surgeries, vascular diseases and accidents in remote places in which use of a manual tourniquet is required. Tissues under such stress suffer the consequences of evidenced by changes in their normal microscopic organization that can be reversible or irreversible according to the time and severity of lesion. An experimental model of ischemia has been designed taking into account the characteristics similar to a surgical procedure, from preparation for anesthesia up to the postsurgical follow up of each animal until it finishes the established time of reperfusion. Two muscles, soleus and extensor carpi radialis longus, dissected from Wistar rats that were underwent to short periods of ischemia and short and prolonged periods of reperfusion up to 32 days. There were no significant changes in the macroscopic weight of muscles, but significant differences were found in the area occupied by intramuscular extracellular matrix. During reperfusion, a partial recovery was observed until the last day of study. If we pretend to extrapolate these results to clinical areas, its importance focuses in the recovering of function and the following up of patients after surgical procedures as studied in the present experiment.10 páginasDigitalengWest Bengal Veterinary ASSOCFacultad de Ciencias de la SaludMedicinaDepartamento de Ciencias Básicas MédicasCalcuttaExploratory Animal and Medical Research, Vol. 6, No.1 -2016EL AUTOR, expresa que la obra objeto de la presente autorización es original y la elaboró sin quebrantar ni suplantar los derechos de autor de terceros, y de tal forma, la obra es de su exclusiva autoría y tiene la titularidad sobre éste. PARÁGRAFO: en caso de queja o acción por parte de un tercero referente a los derechos de autor sobre el artículo, folleto o libro en cuestión, EL AUTOR, asumirá la responsabilidad total, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos, la Universidad Icesi actúa como un tercero de buena fe. Esta autorización, permite a la Universidad Icesi, de forma indefinida, para que en los términos establecidos en la Ley 23 de 1982, la Ley 44 de 1993, leyes y jurisprudencia vigente al respecto, haga publicación de este con fines educativos Todo persona que consulte ya sea la biblioteca o en medio electrónico podrá copiar apartes del texto citando siempre la fuentes, es decir el título del trabajo y el autor.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2IsquemiaReperfusionEnfermedades vascularesCiencias socio biomédicasMedical sciencesImage analysis in Gomori´s trichrome stain of skeletal muscles subjected to ischemia and reperfusion injuryinfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1Artículoinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Editor611525ORIGINALrosero_image_analysis_2016.pdfrosero_image_analysis_2016.pdfapplication/pdf2410247http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/82015/3/rosero_image_analysis_2016.pdf35c813dfdf0d72be352e802f8c9fc2d6MD53LICENSElicense.txtlicense.txttext/plain1748http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/82015/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5210906/82015oai:repository.icesi.edu.co:10906/820152018-10-23 16:23:40.381Biblioteca Digital - Universidad icesicdcriollo@icesi.edu.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 |