AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm
The presence and extent of genetic variation in saffron crocus are still debated, as testified by several contradictory articles providing contrasting results about the monomorphism or less of the species. Remarkably, phenotypic variations have been frequently observed in the field, such variations...
- Autores:
-
Santaella Tenorio, Marcela
Fernández, José Antonio
Roldán, Marta
Santana, Omar
De Los Mozos Pascual, Marcelino
Sánchez, Rosa Ana
Colli, Licia
Busconi, Matteo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad ICESI
- Repositorio:
- Repositorio ICESI
- Idioma:
- eng
- OAI Identifier:
- oai:repository.icesi.edu.co:10906/79857
- Acceso en línea:
- https://doi.org/10.1371/journal.pone.0123434
http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0123434.PDF
http://hdl.handle.net/10906/79857
- Palabra clave:
- Polimorfismo genético
Fenotipo
Biología vegetal
Caracterización morfológica
Fitomejoramiento
Genetica de las plantas
Biology
Crocus sativus
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.title.eng.fl_str_mv |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
title |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
spellingShingle |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm Polimorfismo genético Fenotipo Biología vegetal Caracterización morfológica Fitomejoramiento Genetica de las plantas Biology Crocus sativus |
title_short |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
title_full |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
title_fullStr |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
title_full_unstemmed |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
title_sort |
AFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasm |
dc.creator.fl_str_mv |
Santaella Tenorio, Marcela Fernández, José Antonio Roldán, Marta Santana, Omar De Los Mozos Pascual, Marcelino Sánchez, Rosa Ana Colli, Licia Busconi, Matteo |
dc.contributor.author.spa.fl_str_mv |
Santaella Tenorio, Marcela Fernández, José Antonio Roldán, Marta Santana, Omar De Los Mozos Pascual, Marcelino Sánchez, Rosa Ana Colli, Licia Busconi, Matteo |
dc.subject.spa.fl_str_mv |
Polimorfismo genético Fenotipo Biología vegetal Caracterización morfológica Fitomejoramiento Genetica de las plantas |
topic |
Polimorfismo genético Fenotipo Biología vegetal Caracterización morfológica Fitomejoramiento Genetica de las plantas Biology Crocus sativus |
dc.subject.eng.fl_str_mv |
Biology Crocus sativus |
description |
The presence and extent of genetic variation in saffron crocus are still debated, as testified by several contradictory articles providing contrasting results about the monomorphism or less of the species. Remarkably, phenotypic variations have been frequently observed in the field, such variations are usually unstable and can change from one growing season to another. Considering that gene expression can be influenced both by genetic and epigenetic changes, epigenetics could be a plausible cause of the alternative phenotypes. In order to obtain new insights into this issue, we carried out a molecular marker analysis of 112 accessions from theWorld Saffron and Crocus Collection. The accessions were grown for at least three years in the same open field conditions. The same samples were analysed using Amplified Fragment Length Polymorphism (AFLP) and Methyl Sensitive AFLP in order to search for variation at the genetic (DNA sequence) and epigenetic (cytosine methylation) level. While the genetic variability was low (4.23% polymorphic peaks and twelve (12) effective different genotypes), the methyl sensitive analysis showed the presence of high epigenetic variability (33.57% polymorphic peaks and twenty eight (28) different effective epigenotypes) |
publishDate |
2015 |
dc.date.issued.none.fl_str_mv |
2015-04-17 |
dc.date.accessioned.none.fl_str_mv |
2016-08-30T22:01:48Z |
dc.date.available.none.fl_str_mv |
2016-08-30T22:01:48Z |
dc.type.eng.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coar.none.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.local.spa.fl_str_mv |
Artículo |
dc.type.version.eng.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
dc.identifier.eng.fl_str_mv |
https://doi.org/10.1371/journal.pone.0123434 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.other.spa.fl_str_mv |
http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0123434.PDF |
dc.identifier.uri.eng.fl_str_mv |
http://hdl.handle.net/10906/79857 |
dc.identifier.instname.none.fl_str_mv |
instname: Universidad Icesi |
dc.identifier.reponame.none.fl_str_mv |
reponame: Biblioteca Digital |
dc.identifier.repourl.none.fl_str_mv |
repourl: https://repository.icesi.edu.co/ |
url |
https://doi.org/10.1371/journal.pone.0123434 http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0123434.PDF http://hdl.handle.net/10906/79857 |
identifier_str_mv |
1932-6203 instname: Universidad Icesi reponame: Biblioteca Digital repourl: https://repository.icesi.edu.co/ |
dc.language.iso.eng.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Pilos One, Vol. 10, No. 4 - 2015 |
dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.rights.accessrights.eng.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.license.none.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) |
dc.rights.coar.none.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) http://purl.org/coar/access_right/c_abf2 |
eu_rights_str_mv |
openAccess |
dc.format.extent.spa.fl_str_mv |
17 páginas |
dc.coverage.spatial.eng.fl_str_mv |
San Francisco de Lat: 24 32 00 N degrees minutes Lat: 24.5333 decimal degrees Long: 110 53 00 W degrees minutes Long: -110.8833 decimal degrees |
dc.publisher.eng.fl_str_mv |
Public Library of Science |
dc.publisher.place.spa.fl_str_mv |
San Francisco |
institution |
Universidad ICESI |
bitstream.url.fl_str_mv |
http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/79857/2/santaella_analysis_variation_2015.pdf.txt http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/79857/1/santaella_analysis_variation_2015.pdf |
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cdcriollo@icesi.edu.co |
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1811668478385455104 |
spelling |
Santaella Tenorio, MarcelaFernández, José AntonioRoldán, MartaSantana, OmarDe Los Mozos Pascual, MarcelinoSánchez, Rosa AnaColli, LiciaBusconi, MatteoSan Francisco de Lat: 24 32 00 N degrees minutes Lat: 24.5333 decimal degrees Long: 110 53 00 W degrees minutes Long: -110.8833 decimal degrees2016-08-30T22:01:48Z2016-08-30T22:01:48Z2015-04-17https://doi.org/10.1371/journal.pone.01234341932-6203http://journals.plos.org/plosone/article/asset?id=10.1371/journal.pone.0123434.PDFhttp://hdl.handle.net/10906/79857instname: Universidad Icesireponame: Biblioteca Digitalrepourl: https://repository.icesi.edu.co/The presence and extent of genetic variation in saffron crocus are still debated, as testified by several contradictory articles providing contrasting results about the monomorphism or less of the species. Remarkably, phenotypic variations have been frequently observed in the field, such variations are usually unstable and can change from one growing season to another. Considering that gene expression can be influenced both by genetic and epigenetic changes, epigenetics could be a plausible cause of the alternative phenotypes. In order to obtain new insights into this issue, we carried out a molecular marker analysis of 112 accessions from theWorld Saffron and Crocus Collection. The accessions were grown for at least three years in the same open field conditions. The same samples were analysed using Amplified Fragment Length Polymorphism (AFLP) and Methyl Sensitive AFLP in order to search for variation at the genetic (DNA sequence) and epigenetic (cytosine methylation) level. While the genetic variability was low (4.23% polymorphic peaks and twelve (12) effective different genotypes), the methyl sensitive analysis showed the presence of high epigenetic variability (33.57% polymorphic peaks and twenty eight (28) different effective epigenotypes)17 páginasengPublic Library of ScienceSan FranciscoPilos One, Vol. 10, No. 4 - 2015EL AUTOR, expresa que la obra objeto de la presente autorización es original y la elaboró sin quebrantar ni suplantar los derechos de autor de terceros, y de tal forma, la obra es de su exclusiva autoría y tiene la titularidad sobre éste. PARÁGRAFO: en caso de queja o acción por parte de un tercero referente a los derechos de autor sobre el artículo, folleto o libro en cuestión, EL AUTOR, asumirá la responsabilidad total, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos, la Universidad Icesi actúa como un tercero de buena fe. Esta autorización, permite a la Universidad Icesi, de forma indefinida, para que en los términos establecidos en la Ley 23 de 1982, la Ley 44 de 1993, leyes y jurisprudencia vigente al respecto, haga publicación de este con fines educativos. Toda persona que consulte ya sea la biblioteca o en medio electrónico podrá copiar apartes del texto citando siempre la fuentes, es decir el título del trabajo y el autor.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2Polimorfismo genéticoFenotipoBiología vegetalCaracterización morfológicaFitomejoramientoGenetica de las plantasBiologyCrocus sativusAFLP and MS-AFLP Analysis of the Variation within Saffron Crocus (Crocus sativus L.) Germplasminfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1Artículoinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85104117TEXTsantaella_analysis_variation_2015.pdf.txtsantaella_analysis_variation_2015.pdf.txttext/plain65193http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/79857/2/santaella_analysis_variation_2015.pdf.txtfa8625879e8192588417ba56d2707235MD52ORIGINALsantaella_analysis_variation_2015.pdfsantaella_analysis_variation_2015.pdfapplication/pdf1063226http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/79857/1/santaella_analysis_variation_2015.pdf7150523564ad4c6c7639731a16629330MD5110906/79857oai:repository.icesi.edu.co:10906/798572020-06-11 11:17:35.566Biblioteca Digital - Universidad icesicdcriollo@icesi.edu.co |