Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats
Tumor Necrosis Factor-alpha (TNF-α) is an immunomodulatory and proinflammatory cytokine implicated in neuro-inflammation and neuronal damage in response to cerebral ischemia. The present study tested the hypothesis that anti-TNF-α agents may be protective against cerebral infarction. Transient focal...
- Autores:
-
Arango Dávila, César Augusto
Cardozo, Carlos F.
Londoño, Ana C.
Cañas, Carlos A.
Rengifo, Juliana
Orozco, Jorge Luis
Cañas, Felipe
Echeverri, Andrés F.
Vera, Alejandro
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2015
- Institución:
- Universidad ICESI
- Repositorio:
- Repositorio ICESI
- Idioma:
- eng
- OAI Identifier:
- oai:repository.icesi.edu.co:10906/82290
- Acceso en línea:
- http://www.tandfonline.com/doi/full/10.3109/00207454.2014.980906
http://hdl.handle.net/10906/82290
http://dx.doi.org/10.3109/00207454.2014.980906
- Palabra clave:
- Ratas - Experimentos
Isquemia cerebral
Cerebro
Anticuerpos monoclonales
Infarto cerebral
Ciencias socio biomédicas
Biomedical sciences
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-nd/4.0/
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Arango Dávila, César AugustoCardozo, Carlos F.Londoño, Ana C.Cañas, Carlos A.Rengifo, JulianaOrozco, Jorge LuisCañas, FelipeEcheverri, Andrés F.Vera, AlejandroEstados Unidos de Lat: 25 07 00 N degrees minutes Lat: 25.1167 decimal degrees Long: 100 28 00 W degrees minutes Long: -100.4667 decimal degrees2017-11-16T20:07:43Z2017-11-16T20:07:43Z2015-12-0210.3109/00207454.2014.9809060020-7454http://www.tandfonline.com/doi/full/10.3109/00207454.2014.980906http://hdl.handle.net/10906/82290http://dx.doi.org/10.3109/00207454.2014.980906instname: Universidad Icesireponame: Biblioteca Digitalrepourl: https://repository.icesi.edu.co/Tumor Necrosis Factor-alpha (TNF-α) is an immunomodulatory and proinflammatory cytokine implicated in neuro-inflammation and neuronal damage in response to cerebral ischemia. The present study tested the hypothesis that anti-TNF-α agents may be protective against cerebral infarction. Transient focal ischemia was artificially induced in anesthetized adult male Wistar rats (300–350 g) by middle cerebral artery occlusion (MCAO) with an intraluminal suture. TNF-α function was interfered with either a chimeric monoclonal antibody against TNF-α (infliximab-7 mg/kg) aiming to TNF-α soluble and membrane-attached form; or a chimeric fusion protein of TNF-α receptor-2 with a fragment crystallizable (Fc) region of IgG1 (etanercept-5 mg/kg) aiming for the TNF-α soluble form. Both agents were administered intraperitoneally 0 or 6 h after inducing ischemia. Infarct volume was measured by 2,3,5-triphenyltetrazolium chloride staining. Cerebral infarct volume was significantly reduced in either etanercept or infliximab-treated group compared with non-treated MCAO rats 24 h after reperfusion. These results suggest that anti-TNF-α agents may reduce focal ischemic injury in rats.5 páginasDigitalapplication/pdfengInforma HealthcareFacultad Ciencias de la SaludMedicinaDepartamento de Ciencias Básicas MédicasEstados UnidosInternational Journal of Neuroscience, Vol. 125, No. 12 - 2015EL AUTOR, expresa que la obra objeto de la presente autorización es original y la elaboró sin quebrantar ni suplantar los derechos de autor de terceros, y de tal forma, la obra es de su exclusiva autoría y tiene la titularidad sobre éste. PARÁGRAFO: en caso de queja o acción por parte de un tercero referente a los derechos de autor sobre el artículo, folleto o libro en cuestión, EL AUTOR, asumirá la responsabilidad total, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos, la Universidad Icesi actúa como un tercero de buena fe. Esta autorización, permite a la Universidad Icesi, de forma indefinida, para que en los términos establecidos en la Ley 23 de 1982, la Ley 44 de 1993, leyes y jurisprudencia vigente al respecto, haga publicación de este con fines educativos. Toda persona que consulte ya sea la biblioteca o en medio electrónico podrá copiar apartes del texto citando siempre la fuentes, es decir el título del trabajo y el autor.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)http://purl.org/coar/access_right/c_abf2Ratas - ExperimentosIsquemia cerebralCerebroAnticuerpos monoclonalesInfarto cerebralCiencias socio biomédicasBiomedical sciencesSoluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in ratsinfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1Artículoinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85Comunidad Universidad Icesi – Investigadores12512936940ORIGINALdocumento.htmldocumento.htmltext/html295http://repository.icesi.edu.co/biblioteca_digital/bitstream/10906/82290/1/documento.html1cff8d22320853d904e019ea5567f7b5MD5110906/82290oai:repository.icesi.edu.co:10906/822902020-05-07 19:41:51.483Biblioteca Digital - Universidad icesicdcriollo@icesi.edu.co |
| dc.title.none.fl_str_mv |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| title |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| spellingShingle |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats Ratas - Experimentos Isquemia cerebral Cerebro Anticuerpos monoclonales Infarto cerebral Ciencias socio biomédicas Biomedical sciences |
| title_short |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| title_full |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| title_fullStr |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| title_full_unstemmed |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| title_sort |
Soluble or soluble/membrane TNF-± inhibitors protect the brain from focal ischemic injury in rats |
| dc.creator.fl_str_mv |
Arango Dávila, César Augusto Cardozo, Carlos F. Londoño, Ana C. Cañas, Carlos A. Rengifo, Juliana Orozco, Jorge Luis Cañas, Felipe Echeverri, Andrés F. Vera, Alejandro |
| dc.contributor.author.spa.fl_str_mv |
Arango Dávila, César Augusto Cardozo, Carlos F. Londoño, Ana C. Cañas, Carlos A. Rengifo, Juliana Orozco, Jorge Luis Cañas, Felipe Echeverri, Andrés F. Vera, Alejandro |
| dc.subject.spa.fl_str_mv |
Ratas - Experimentos Isquemia cerebral Cerebro Anticuerpos monoclonales Infarto cerebral Ciencias socio biomédicas |
| topic |
Ratas - Experimentos Isquemia cerebral Cerebro Anticuerpos monoclonales Infarto cerebral Ciencias socio biomédicas Biomedical sciences |
| dc.subject.eng.fl_str_mv |
Biomedical sciences |
| description |
Tumor Necrosis Factor-alpha (TNF-α) is an immunomodulatory and proinflammatory cytokine implicated in neuro-inflammation and neuronal damage in response to cerebral ischemia. The present study tested the hypothesis that anti-TNF-α agents may be protective against cerebral infarction. Transient focal ischemia was artificially induced in anesthetized adult male Wistar rats (300–350 g) by middle cerebral artery occlusion (MCAO) with an intraluminal suture. TNF-α function was interfered with either a chimeric monoclonal antibody against TNF-α (infliximab-7 mg/kg) aiming to TNF-α soluble and membrane-attached form; or a chimeric fusion protein of TNF-α receptor-2 with a fragment crystallizable (Fc) region of IgG1 (etanercept-5 mg/kg) aiming for the TNF-α soluble form. Both agents were administered intraperitoneally 0 or 6 h after inducing ischemia. Infarct volume was measured by 2,3,5-triphenyltetrazolium chloride staining. Cerebral infarct volume was significantly reduced in either etanercept or infliximab-treated group compared with non-treated MCAO rats 24 h after reperfusion. These results suggest that anti-TNF-α agents may reduce focal ischemic injury in rats. |
| publishDate |
2015 |
| dc.date.issued.none.fl_str_mv |
2015-12-02 |
| dc.date.accessioned.none.fl_str_mv |
2017-11-16T20:07:43Z |
| dc.date.available.none.fl_str_mv |
2017-11-16T20:07:43Z |
| dc.type.eng.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
| dc.type.local.spa.fl_str_mv |
Artículo |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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10.3109/00207454.2014.980906 |
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0020-7454 |
| dc.identifier.other.none.fl_str_mv |
http://www.tandfonline.com/doi/full/10.3109/00207454.2014.980906 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10906/82290 |
| dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.3109/00207454.2014.980906 |
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instname: Universidad Icesi |
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reponame: Biblioteca Digital |
| dc.identifier.repourl.none.fl_str_mv |
repourl: https://repository.icesi.edu.co/ |
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10.3109/00207454.2014.980906 0020-7454 instname: Universidad Icesi reponame: Biblioteca Digital repourl: https://repository.icesi.edu.co/ |
| url |
http://www.tandfonline.com/doi/full/10.3109/00207454.2014.980906 http://hdl.handle.net/10906/82290 http://dx.doi.org/10.3109/00207454.2014.980906 |
| dc.language.iso.eng.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
International Journal of Neuroscience, Vol. 125, No. 12 - 2015 |
| dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.accessrights.eng.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.license.none.fl_str_mv |
Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) |
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http://purl.org/coar/access_right/c_abf2 |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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5 páginas |
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Digital |
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application/pdf |
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Estados Unidos de Lat: 25 07 00 N degrees minutes Lat: 25.1167 decimal degrees Long: 100 28 00 W degrees minutes Long: -100.4667 decimal degrees |
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Informa Healthcare |
| dc.publisher.faculty.spa.fl_str_mv |
Facultad Ciencias de la Salud |
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Medicina |
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Departamento de Ciencias Básicas Médicas |
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Estados Unidos |
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Universidad ICESI |
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