Isolation and phenotypic characterization of tumor cells of patients with a diagnosis of ovarian cancer

Ovarian cancer is the fifth leading cause of cancer‐related deaths. It causes approximately 125,000 deaths per year worldwide; its diagnosis is made in advanced stages resulting in a high mortality rate. The objective of the study was optimizing the isolation of cells obtained from the solid tumor and ascitic fluid of patients with ovarian cancer and the phenotype with markers related to the epithelial–mesenchymal transition. For this, the solid tumor tissue was disaggregated and cultivated with different methodologies. As a result, cell growth was obtained and epi‐immunofluorescence was performed using antibodies against E‐cadherin, EpCAM, N‐cadherin, vimentin, CD133, and CD44. The primary culture from the solid tumor was obtained using Dispase II and DMEM/F12. Finally, heterogeneity was detected in terms of the expression of mesenchymal and epithelial type markers in the two types of isolated cells. Additionally, CD133 and CD44

• Autores: Gutiérrez Castañeda, Luz D.; Tovar Parra, David; Quintero, Gloria; Amezquita, Lorena; Guerrero, Carlos; Sanabria, Daniel
• Tipo de recurso: Article of journal
• Fecha de publicación: 2019
• Institución: Fundación Universitaria de Ciencias de la Salud - FUCS
• Repositorio: Repositorio Digital Institucional ReDi
• Idioma: eng
• OAI Identifier: oai:repositorio.fucsalud.edu.co:001/2683
• Acceso en línea: https://repositorio.fucsalud.edu.co/handle/001/2683
• Palabra clave: Epithelial; Mesenchyma; Ovarian cancer; Tumor; Neoplasias ovarianas; Mesodermo; Epitelial
• Rights: openAccess
• License: Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)