Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero

Los puntos de carbono (PC) son nanoparículas a base de carbono, con diámetros de 10 nm en promedio. Se destacan por sus propiedades fluorescentes, lo que ha permitido plantear su aplicación en el desarrollo de técnicas de bioimagenología y radioterapia. No obstante, pueden utilizarse también en otra...

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Autores:
Lancheros Vega, María Camila
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2021
Institución:
Escuela Colombiana de Ingeniería Julio Garavito
Repositorio:
Repositorio Institucional ECI
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spa
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oai:repositorio.escuelaing.edu.co:001/1535
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https://repositorio.escuelaing.edu.co/handle/001/1535
https://catalogo.escuelaing.edu.co/cgi-bin/koha/opac-detail.pl?biblionumber=22638
Palabra clave:
Puntos de carbono- Células
Ingeniería Biomédica
Lineas celulares
Citotoxicidad
Puntos de carbono
Cellular lines
Cytotoxicity
Carbon dots
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openAccess
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dc.title.spa.fl_str_mv Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
title Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
spellingShingle Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
Puntos de carbono- Células
Ingeniería Biomédica
Lineas celulares
Citotoxicidad
Puntos de carbono
Cellular lines
Cytotoxicity
Carbon dots
title_short Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
title_full Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
title_fullStr Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
title_full_unstemmed Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
title_sort Evaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y vero
dc.creator.fl_str_mv Lancheros Vega, María Camila
dc.contributor.advisor.none.fl_str_mv Rodríguez Burbano, Diana Consuelo
Ondo Méndez, Alejandro Oyono
dc.contributor.author.none.fl_str_mv Lancheros Vega, María Camila
dc.subject.armarc.none.fl_str_mv Puntos de carbono- Células
Ingeniería Biomédica
topic Puntos de carbono- Células
Ingeniería Biomédica
Lineas celulares
Citotoxicidad
Puntos de carbono
Cellular lines
Cytotoxicity
Carbon dots
dc.subject.proposal.spa.fl_str_mv Lineas celulares
Citotoxicidad
Puntos de carbono
dc.subject.proposal.eng.fl_str_mv Cellular lines
Cytotoxicity
Carbon dots
description Los puntos de carbono (PC) son nanoparículas a base de carbono, con diámetros de 10 nm en promedio. Se destacan por sus propiedades fluorescentes, lo que ha permitido plantear su aplicación en el desarrollo de técnicas de bioimagenología y radioterapia. No obstante, pueden utilizarse también en otras aplicaciones como la liberación controlada de fármacos y los biosensores. Dado su alto valor en técnicas de diagnóstico y tratamiento del cáncer, cuando se habla de la toxicidad intrínseca de este material, la literatura se ha preocupado mayormente por determinar su citotoxicidad en células cancerosas. Sin embargo, teniendo en cuenta que los PC podrían acumularse también en órganos sanos o en tejido sano que rodea el tumor, resulta de capital importancia determinar su toxicidad en células sanas. En consecuencia, como objetivo de este proyecto se planteó sintetizar PC y determinar citotoxicidad en las líneas celulares derivadas de tejido sano 3T3-L1 (preadipocitos) y Vero (riñón). Para ello se sintetizaron puntos de carbono a partir de ácido cítrico como precursor y etanol y N, N-Dimetilformamida. La citotoxicidad se determinó con los ensayos de Azul Tripán y MTT. Se establecieron dos controles uno positivo (tóxico) y uno negativo (no tóxico). Las pruebas estadísticas indicaron que los PC no mostraron citotoxicidad detectable en las células tumores a concentraciones entre 50 y 500 μg/mL. Con la realización de este trabajo se establecieron las bases de la citotoxicidad de una nanoplataforma de PC en su primera etapa de desarrollo, cuyo fin último será la aplicación de radioterapia.
publishDate 2021
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2021-10-01T14:29:56Z
dc.date.available.none.fl_str_mv 2021-05-31
2021-10-01T14:29:56Z
dc.date.issued.none.fl_str_mv 2021
dc.type.spa.fl_str_mv Trabajo de grado - Pregrado
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spelling Rodríguez Burbano, Diana Consuelocb61f73e8b008cae082ca7db0b1cc60d300Ondo Méndez, Alejandro Oyonoff4d79b010dec1da57890f36e7c49b31300Lancheros Vega, María Camila83dafe66960f825f516c199df223be486002021-05-31T20:13:22Z2021-10-01T14:29:56Z2021-05-312021-10-01T14:29:56Z2021https://repositorio.escuelaing.edu.co/handle/001/1535https://catalogo.escuelaing.edu.co/cgi-bin/koha/opac-detail.pl?biblionumber=22638Los puntos de carbono (PC) son nanoparículas a base de carbono, con diámetros de 10 nm en promedio. Se destacan por sus propiedades fluorescentes, lo que ha permitido plantear su aplicación en el desarrollo de técnicas de bioimagenología y radioterapia. No obstante, pueden utilizarse también en otras aplicaciones como la liberación controlada de fármacos y los biosensores. Dado su alto valor en técnicas de diagnóstico y tratamiento del cáncer, cuando se habla de la toxicidad intrínseca de este material, la literatura se ha preocupado mayormente por determinar su citotoxicidad en células cancerosas. Sin embargo, teniendo en cuenta que los PC podrían acumularse también en órganos sanos o en tejido sano que rodea el tumor, resulta de capital importancia determinar su toxicidad en células sanas. En consecuencia, como objetivo de este proyecto se planteó sintetizar PC y determinar citotoxicidad en las líneas celulares derivadas de tejido sano 3T3-L1 (preadipocitos) y Vero (riñón). Para ello se sintetizaron puntos de carbono a partir de ácido cítrico como precursor y etanol y N, N-Dimetilformamida. La citotoxicidad se determinó con los ensayos de Azul Tripán y MTT. Se establecieron dos controles uno positivo (tóxico) y uno negativo (no tóxico). Las pruebas estadísticas indicaron que los PC no mostraron citotoxicidad detectable en las células tumores a concentraciones entre 50 y 500 μg/mL. Con la realización de este trabajo se establecieron las bases de la citotoxicidad de una nanoplataforma de PC en su primera etapa de desarrollo, cuyo fin último será la aplicación de radioterapia.Carbon Dots (CDs) are carbon based nanoparticles with average diameters of 10 nm. They are distinguished for their fluorescent properties, which has allowed their application in the development of techniques for bioimage and radiotherapy. Nevertheless they have other applications such as controlled drug release and biosensors. Given their importance in techniques for treatment and diagnostic, when it comes to their intrinsic toxicity literature has worried more about determining cytotoxicity on cancer cell lines. Taking into account that CDs may accumulate in healthy organs or tissue that surrounds tumors, it is of great importance to determine their cytotoxicity on healthy cell lines. As a consequence, the objective of this project was to determine the cytotoxicity of CDs on the two cell lineages derived from healthy tissue 3T3-L1 (preadipocytes) and Vero (Kidney). For this purpose, carbon dots were synthesized using citric acid as precursor and ethanol and N, N-Dimethylformamide as solvents, cytotoxicity was measured using the Trypan Blue and MTT assays, two controls were stablished a positive control (toxic) and a negative control (non-toxic). The statistical analysis did not show detectable cytotoxicity at concentrations of CDs in the range from 50 to 500 μg/mL. With this thesis work the bases of the cytotoxicity of a nanoplatform of carbon dots in the first stage of development were established, whose final purpose is to create a theranostic platform for radiotherapy.1. INTRODUCCIÓN ........................................................................................................ 4 1.1. Principales aplicaciones biomédicas de los puntos de carbono ........................... 6 1.1.1. Puntos de carbono para bioimagen .............................................................. 7 1.1.2. Puntos de carbono para liberación de fármacos ........................................... 8 1.1.3. Puntos de carbono para radioterapia ............................................................ 9 1.2. Ensayos de citotoxicidad ................................................................................... 10 1.2.1. Ensayos de exclusión de colorante ................................................................. 10 1.2.2. Ensayos colorimétricos ................................................................................... 10 1.2.3. Ensayos fluorométricos ................................................................................... 11 1.2.4. Ensayos luminométricos de ATP .................................................................... 11 1.3. Citotoxicidad de los puntos de carbono ............................................................. 11 2. OBJETIVOS ............................................................................................................. 16 2.1. General .............................................................................................................. 16 2.2. Específicos ........................................................................................................ 16 3. METODOLOGÍA EXPERIMENTAL ........................................................................... 17 3.1. Síntesis de puntos de carbono........................................................................... 17 3.2. Evaluación de la citotoxicidad de los puntos de carbono ................................... 18 3.2.1. Líneas celulares ......................................................................................... 18 3.2.2. Exposición de líneas celulares a puntos de carbono................................... 18 3.2.3. Ensayo de citotoxicidad por Azul Tripán ..................................................... 19 3.2.4. Ensayo de citotoxicidad por MTT ................................................................ 19 3.3. Análisis estadístico ............................................................................................ 20 4. RESULTADOS ......................................................................................................... 21 4.1. Síntesis de puntos de carbono ....................................................................... 21 4.2. Ensayo de citotoxicidad por Azul Tripán ......................................................... 21 4.3. Ensayo de citotoxicidad por MTT ................................................................... 24 5. DISCUSIÓN .............................................................................................................. 26 6. RECOMENDACIONES Y TRABAJOS FUTUROS .................................................... 28 7. CONCLUSIONES ..................................................................................................... 29PregradoIngeniero(a) Biomédico(a)Radioterapia y nanomateriales32 páginasapplication/pdfspaEvaluación del efecto citotóxico de puntos de carbono en células 3T3-L1 y veroTrabajo de grado - Pregradoinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_7a1fTextinfo:eu-repo/semantics/bachelorThesishttps://purl.org/redcol/resource_type/TPhttp://purl.org/coar/version/c_970fb48d4fbd8a85Ingeniería BiomédicaN/AJ. Jeevanandam, A. Barhoum, Y. S. Chan, A. Dufresne, and M. K. Danquah, “Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations,” Beilstein J. Nanotechnol, vol. 9, pp. 1050–1074, 2018, doi: 10.3762/bjnano.9.98.G. Guisbiers, S. Mejía-Rosales, and F. Leonard Deepak, “Nanomaterial properties: Size and shape dependencies,” Journal of Nanomaterials, vol. 2012, 2012, doi: 10.1155/2012/180976.V. Francia, D. Montizaan, and A. Salvati, “Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine,” Beilstein Journal of Nanotechnology, vol. 11, no. 1, pp. 338–353, Feb. 2020, doi: 10.3762/bjnano.11.25.V. J. Mohanraj and Y. Chen, “Nanoparticles - A review,” Tropical Journal of Pharmaceutical Research, vol. 5, no. 1, pp. 561–573, 2007, doi: 10.4314/tjpr.v5i1.14634C. Contini, M. Schneemilch, S. Gaisford, and N. Quirke, “Nanoparticle–membrane interactions,” Journal of Experimental Nanoscience, vol. 13, no. 1, Jan. 2018, doi: 10.1080/17458080.2017.1413253.J. Fan, M. Claudel, C. Ronzani, Y. Arezki, L. Lebeau, and F. 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Dias, “The Kidney Vero-E6 Cell Line: A Suitable Model to Study the Toxicity of Microcystins,” in New Insights into Toxicity and Drug Testing, InTech, 2013.info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Puntos de carbono- CélulasIngeniería BiomédicaLineas celularesCitotoxicidadPuntos de carbonoCellular linesCytotoxicityCarbon dotsLICENSElicense.txttext/plain1881https://repositorio.escuelaing.edu.co/bitstream/001/1535/1/license.txt5a7ca94c2e5326ee169f979d71d0f06eMD51open accessORIGINALAutorizacion.pdfapplication/pdf926718https://repositorio.escuelaing.edu.co/bitstream/001/1535/2/Autorizacion.pdfc06e09b6152e9862659b9e2111237ba6MD52metadata only accessLancheros Vega, María Camila-2021.pdfapplication/pdf868473https://repositorio.escuelaing.edu.co/bitstream/001/1535/3/Lancheros%20Vega%2c%20Mar%c3%ada%20Camila-2021.pdfbef132a4f048a5944a14af04a73cdbb1MD53open accessTEXTAutorizacion.pdf.txtAutorizacion.pdf.txtExtracted texttext/plain3411https://repositorio.escuelaing.edu.co/bitstream/001/1535/4/Autorizacion.pdf.txtf313facfbae31b430a724a0d88e59610MD54metadata only accessLancheros Vega, María Camila-2021.pdf.txtLancheros Vega, María Camila-2021.pdf.txtExtracted texttext/plain72273https://repositorio.escuelaing.edu.co/bitstream/001/1535/6/Lancheros%20Vega%2c%20Mar%c3%ada%20Camila-2021.pdf.txtbce0aca447a2efd4930bf215653c8608MD56open accessTHUMBNAILAutorizacion.pdf.jpgAutorizacion.pdf.jpgGenerated Thumbnailimage/jpeg13290https://repositorio.escuelaing.edu.co/bitstream/001/1535/5/Autorizacion.pdf.jpgf5eaaa784fb603bb09411534e593f3e0MD55metadata only accessLancheros Vega, María Camila-2021.pdf.jpgLancheros Vega, María Camila-2021.pdf.jpgGenerated Thumbnailimage/jpeg7146https://repositorio.escuelaing.edu.co/bitstream/001/1535/7/Lancheros%20Vega%2c%20Mar%c3%ada%20Camila-2021.pdf.jpg615668ab126cd7f4d62b659aab842e61MD57open access001/1535oai:repositorio.escuelaing.edu.co:001/15352022-04-28 13:37:50.804open accessRepositorio Escuela Colombiana de Ingeniería Julio Garavitorepositorio.eci@escuelaing.edu.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