In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes

Leishmaniasis, caused by Leishmania sp., is one of the mean reason of considerable mortality and morbidity throughout the world, especially in the tropics. Cutaneous and mucocutaneous manifestations are caused by Leishmania braziliensis, and the cutaneous form is the most common one in Colombia. In...

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Tipo de recurso:
Fecha de publicación:
2008
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/23605
Acceso en línea:
https://doi.org/10.1590/S0102-695X2008000300007
https://repository.urosario.edu.co/handle/10336/23605
Palabra clave:
Alkaloids
Amastigote
Apocynaceae
Cytotoxicity
Ervatamia coronaria
Leishmania
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License
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spelling 90f14b5c-a3c7-4b71-845c-a4dad5c62d43-1a57b3363-9cd4-4a44-a8d0-82834625ddeb-1030cc350-f23f-44aa-ba8e-9d9bf1ceb3f0-12020-05-26T00:03:33Z2020-05-26T00:03:33Z2008Leishmaniasis, caused by Leishmania sp., is one of the mean reason of considerable mortality and morbidity throughout the world, especially in the tropics. Cutaneous and mucocutaneous manifestations are caused by Leishmania braziliensis, and the cutaneous form is the most common one in Colombia. In the search for antileishmanial compounds from natural sources, we studied the alkaloids mixture from Ervatamia coronaria against L. braziliensis at six different concentrations (1.0, 10, 20, 25, 50 and 100 ?g/mL). Macrophages J774 infected with L. braziliensis were treated with alkaloids one hour, and once a day for three days, after parasitic infection and preserving the same culture medium. Cytotoxicity with trypan blue was undertaken in macrophages J774 by using the same concentrations. Three different cultures samples were carried out. As a control we used medium alone. The alkaloids mix showed a dose/dependent activity on amastigote, but by increasing concentrations from 50 to 100 ?g/mL for three days, we saw a high index of infection, probably caused by cellular death. We did not see any toxic effect on macrophages J774 at 100 ?g/mL, LD50/24h= 233.52 ?g/mL. These results revealed a novel pharmacological activity of alkaloids from E. coronaria against amastigotes of L. braziliensis IC50 = 2.6 and 12.4 ?g/mL without toxicity on host cells.application/pdfhttps://doi.org/10.1590/S0102-695X2008000300007https://repository.urosario.edu.co/handle/10336/23605spa355No. 3350Brazilian Journal of PharmacognosyVol. 18Brazilian Journal of Pharmacognosy, Vol.18, No.3 (2008); pp. 350-355https://www.scopus.com/inward/record.uri?eid=2-s2.0-55849092895&doi=10.1590%2fS0102-695X2008000300007&partnerID=40&md5=53b4b2449d899f43152ac2624281c3aaAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAlkaloidsAmastigoteApocynaceaeCytotoxicityErvatamia coronariaLeishmaniaIn vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotesActividad in vitro de la mezcla de alcaloides de Ervatamia coronaria (Jacq) Staff. Apocynaceae sobre amastigotes de Leishmania braziliensisarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Rodríguez, Amanda MorenoCamargo, Jorge RoblesGarcía, Felio J. BelloORIGINALa07v18n3.pdfapplication/pdf172350https://repository.urosario.edu.co/bitstreams/223917ae-8f7f-4d63-8723-4a1ace517956/download1d844f614d504358423a248fee7131e5MD51TEXTa07v18n3.pdf.txta07v18n3.pdf.txtExtracted texttext/plain30718https://repository.urosario.edu.co/bitstreams/f52274ae-5f69-4ecc-82d6-5b54c50ab603/download7c06907c4d88f14ed96cbd7caeaae8f7MD52THUMBNAILa07v18n3.pdf.jpga07v18n3.pdf.jpgGenerated Thumbnailimage/jpeg4840https://repository.urosario.edu.co/bitstreams/79d01ce2-bcd8-46f8-ab06-db00f084840f/download52523d6f41170ad5e98caba722076644MD5310336/23605oai:repository.urosario.edu.co:10336/236052022-05-02 07:37:14.747939https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
dc.title.TranslatedTitle.eng.fl_str_mv Actividad in vitro de la mezcla de alcaloides de Ervatamia coronaria (Jacq) Staff. Apocynaceae sobre amastigotes de Leishmania braziliensis
title In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
spellingShingle In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
Alkaloids
Amastigote
Apocynaceae
Cytotoxicity
Ervatamia coronaria
Leishmania
title_short In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
title_full In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
title_fullStr In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
title_full_unstemmed In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
title_sort In vitro activity of the alkaloids mixture of Ervatamia coronaria (Jacq) Staff. Apocynaceae on Leishmania braziliensis amastigotes
dc.subject.keyword.spa.fl_str_mv Alkaloids
Amastigote
Apocynaceae
Cytotoxicity
Ervatamia coronaria
Leishmania
topic Alkaloids
Amastigote
Apocynaceae
Cytotoxicity
Ervatamia coronaria
Leishmania
description Leishmaniasis, caused by Leishmania sp., is one of the mean reason of considerable mortality and morbidity throughout the world, especially in the tropics. Cutaneous and mucocutaneous manifestations are caused by Leishmania braziliensis, and the cutaneous form is the most common one in Colombia. In the search for antileishmanial compounds from natural sources, we studied the alkaloids mixture from Ervatamia coronaria against L. braziliensis at six different concentrations (1.0, 10, 20, 25, 50 and 100 ?g/mL). Macrophages J774 infected with L. braziliensis were treated with alkaloids one hour, and once a day for three days, after parasitic infection and preserving the same culture medium. Cytotoxicity with trypan blue was undertaken in macrophages J774 by using the same concentrations. Three different cultures samples were carried out. As a control we used medium alone. The alkaloids mix showed a dose/dependent activity on amastigote, but by increasing concentrations from 50 to 100 ?g/mL for three days, we saw a high index of infection, probably caused by cellular death. We did not see any toxic effect on macrophages J774 at 100 ?g/mL, LD50/24h= 233.52 ?g/mL. These results revealed a novel pharmacological activity of alkaloids from E. coronaria against amastigotes of L. braziliensis IC50 = 2.6 and 12.4 ?g/mL without toxicity on host cells.
publishDate 2008
dc.date.created.spa.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:03:33Z
dc.date.available.none.fl_str_mv 2020-05-26T00:03:33Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
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dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23605
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https://repository.urosario.edu.co/handle/10336/23605
dc.language.iso.spa.fl_str_mv spa
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dc.relation.citationEndPage.none.fl_str_mv 355
dc.relation.citationIssue.none.fl_str_mv No. 3
dc.relation.citationStartPage.none.fl_str_mv 350
dc.relation.citationTitle.none.fl_str_mv Brazilian Journal of Pharmacognosy
dc.relation.citationVolume.none.fl_str_mv Vol. 18
dc.relation.ispartof.spa.fl_str_mv Brazilian Journal of Pharmacognosy, Vol.18, No.3 (2008); pp. 350-355
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