Gene expression and chromosomal location for susceptibility to Sjögren's syndrome

Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling i...

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Autores:
Tipo de recurso:
Fecha de publicación:
2009
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/22251
Acceso en línea:
https://doi.org/10.1016/j.jaut.2009.05.001
https://repository.urosario.edu.co/handle/10336/22251
Palabra clave:
Complementary DNA
Genomic DNA
Interferon
RNA
Adult
Aged
Apoptosis
Article
Chromosomal localization
Controlled study
DNA microarray
Epithelium cell
Female
Gene amplification
Gene expression profiling
Gene expression regulation
Gene locus
Gene overexpression
Genetic susceptibility
Genome analysis
Human
Human cell
Major clinical study
Microsatellite marker
Priority journal
Reverse transcription polymerase chain reaction
RNA hybridization
Salivary gland
Signal transduction
Sjoegren syndrome
Adult
Aged
Alleles
Epithelial Cells
Female
Gene Expression
Gene Expression Profiling
Gene Frequency
Genetic Predisposition to Disease
Genome-Wide Association Study
Genotype
Humans
Microsatellite Repeats
Middle Aged
Salivary Glands
Sjogren's Syndrome
Apoptosis
Cdna microarray
Epithelial cells
Genome-wide association study
Interferon
Sjögren's syndrome
Human
Chromosomes
Rights
License
Abierto (Texto Completo)
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repository_id_str
dc.title.spa.fl_str_mv Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
title Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
spellingShingle Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
Complementary DNA
Genomic DNA
Interferon
RNA
Adult
Aged
Apoptosis
Article
Chromosomal localization
Controlled study
DNA microarray
Epithelium cell
Female
Gene amplification
Gene expression profiling
Gene expression regulation
Gene locus
Gene overexpression
Genetic susceptibility
Genome analysis
Human
Human cell
Major clinical study
Microsatellite marker
Priority journal
Reverse transcription polymerase chain reaction
RNA hybridization
Salivary gland
Signal transduction
Sjoegren syndrome
Adult
Aged
Alleles
Epithelial Cells
Female
Gene Expression
Gene Expression Profiling
Gene Frequency
Genetic Predisposition to Disease
Genome-Wide Association Study
Genotype
Humans
Microsatellite Repeats
Middle Aged
Salivary Glands
Sjogren's Syndrome
Apoptosis
Cdna microarray
Epithelial cells
Genome-wide association study
Interferon
Sjögren's syndrome
Human
Chromosomes
title_short Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
title_full Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
title_fullStr Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
title_full_unstemmed Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
title_sort Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
dc.subject.keyword.spa.fl_str_mv Complementary DNA
Genomic DNA
Interferon
RNA
Adult
Aged
Apoptosis
Article
Chromosomal localization
Controlled study
DNA microarray
Epithelium cell
Female
Gene amplification
Gene expression profiling
Gene expression regulation
Gene locus
Gene overexpression
Genetic susceptibility
Genome analysis
Human
Human cell
Major clinical study
Microsatellite marker
Priority journal
Reverse transcription polymerase chain reaction
RNA hybridization
Salivary gland
Signal transduction
Sjoegren syndrome
Adult
Aged
Alleles
Epithelial Cells
Female
Gene Expression
Gene Expression Profiling
Gene Frequency
Genetic Predisposition to Disease
Genome-Wide Association Study
Genotype
Humans
Microsatellite Repeats
Middle Aged
Salivary Glands
Sjogren's Syndrome
Apoptosis
Cdna microarray
Epithelial cells
Genome-wide association study
Interferon
Sjögren's syndrome
topic Complementary DNA
Genomic DNA
Interferon
RNA
Adult
Aged
Apoptosis
Article
Chromosomal localization
Controlled study
DNA microarray
Epithelium cell
Female
Gene amplification
Gene expression profiling
Gene expression regulation
Gene locus
Gene overexpression
Genetic susceptibility
Genome analysis
Human
Human cell
Major clinical study
Microsatellite marker
Priority journal
Reverse transcription polymerase chain reaction
RNA hybridization
Salivary gland
Signal transduction
Sjoegren syndrome
Adult
Aged
Alleles
Epithelial Cells
Female
Gene Expression
Gene Expression Profiling
Gene Frequency
Genetic Predisposition to Disease
Genome-Wide Association Study
Genotype
Humans
Microsatellite Repeats
Middle Aged
Salivary Glands
Sjogren's Syndrome
Apoptosis
Cdna microarray
Epithelial cells
Genome-wide association study
Interferon
Sjögren's syndrome
Human
Chromosomes
dc.subject.keyword.eng.fl_str_mv Human
Chromosomes
description Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling in fractions enriched in epithelial cells from labial salivary glands (LSGs) of patients with primary SS and identify chromosomal regions harboring susceptibility genes expressed in epithelial cells. A combined approach of gene expression and genome-wide association study was used. Enriched epithelial cell fractions were obtained from LSGs of patients and controls. Amplified total RNA was labeled and hybridized to 10K cDNA microarrays. Results were normalized and subjected to statistical and functional analysis. A genome-wide microsatellite screen at 10 cM resolution (393 markers) was performed. In salivary gland-epithelial cells from patients 528 genes were expressed differentially in comparison to controls. Pathways not previously linked to disease were found to be altered. Twenty-eight and 15 genes associated with apoptosis were up-regulated and down regulated, respectively. Interferon-related genes, most of which participated in interferon signaling, were also found to be up-regulated. From the genome-wide screen, 6 markers showed evidence of highly significant association with the disease. Of these, five loci harbor genes differentially expressed in patients LSG-epithelial cells. Our results show that in enriched gland-epithelial cells of pSS, both pro-apoptotic/anti-apoptotic and interferon signaling inhibition/stimulation balances may occur. Genes found over-expressed in epithelial cells are candidates for disease susceptibility. © 2009 Elsevier Ltd. All rights reserved.
publishDate 2009
dc.date.created.spa.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2020-05-25T23:55:53Z
dc.date.available.none.fl_str_mv 2020-05-25T23:55:53Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.jaut.2009.05.001
dc.identifier.issn.none.fl_str_mv 10959157
08968411
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/22251
url https://doi.org/10.1016/j.jaut.2009.05.001
https://repository.urosario.edu.co/handle/10336/22251
identifier_str_mv 10959157
08968411
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 108
dc.relation.citationIssue.none.fl_str_mv No. 2
dc.relation.citationStartPage.none.fl_str_mv 99
dc.relation.citationTitle.none.fl_str_mv Journal of Autoimmunity
dc.relation.citationVolume.none.fl_str_mv Vol. 33
dc.relation.ispartof.spa.fl_str_mv Journal of Autoimmunity, ISSN:10959157, 08968411, Vol.33, No.2 (2009); pp. 99-108
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spelling fd7acce8-5400-4f35-85d5-0ceb85e062b719474778600da634d6f-b330-4658-b68f-577d2d50aab91b9bfe95-287d-4265-804c-6b6208a0143998037345-9c84-497e-89fe-829e8e0219f5fe958c95-471d-4081-bfc2-72346f89e56d2e6809ce-182a-4cde-9e6e-14dc7f3c27d9fb66c393-0261-47e3-b133-7215a6765bcf1edea94f-bf14-4f04-8374-ee09955af8e45f45f8d5-2c4c-4387-9501-2a7d6720cc42a94a1783-6ef4-4e90-8ff0-83ffa188cd6390d0f62a-6282-4eab-9658-bf09d3a6e0452020-05-25T23:55:53Z2020-05-25T23:55:53Z2009Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling in fractions enriched in epithelial cells from labial salivary glands (LSGs) of patients with primary SS and identify chromosomal regions harboring susceptibility genes expressed in epithelial cells. A combined approach of gene expression and genome-wide association study was used. Enriched epithelial cell fractions were obtained from LSGs of patients and controls. Amplified total RNA was labeled and hybridized to 10K cDNA microarrays. Results were normalized and subjected to statistical and functional analysis. A genome-wide microsatellite screen at 10 cM resolution (393 markers) was performed. In salivary gland-epithelial cells from patients 528 genes were expressed differentially in comparison to controls. Pathways not previously linked to disease were found to be altered. Twenty-eight and 15 genes associated with apoptosis were up-regulated and down regulated, respectively. Interferon-related genes, most of which participated in interferon signaling, were also found to be up-regulated. From the genome-wide screen, 6 markers showed evidence of highly significant association with the disease. Of these, five loci harbor genes differentially expressed in patients LSG-epithelial cells. Our results show that in enriched gland-epithelial cells of pSS, both pro-apoptotic/anti-apoptotic and interferon signaling inhibition/stimulation balances may occur. Genes found over-expressed in epithelial cells are candidates for disease susceptibility. © 2009 Elsevier Ltd. All rights reserved.application/pdfhttps://doi.org/10.1016/j.jaut.2009.05.0011095915708968411https://repository.urosario.edu.co/handle/10336/22251eng108No. 299Journal of AutoimmunityVol. 33Journal of Autoimmunity, ISSN:10959157, 08968411, Vol.33, No.2 (2009); pp. 99-108https://www.scopus.com/inward/record.uri?eid=2-s2.0-68249087935&doi=10.1016%2fj.jaut.2009.05.001&partnerID=40&md5=2e474b671c82dc5adc055d850fd33f2eAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementary DNAGenomic DNAInterferonRNAAdultAgedApoptosisArticleChromosomal localizationControlled studyDNA microarrayEpithelium cellFemaleGene amplificationGene expression profilingGene expression regulationGene locusGene overexpressionGenetic susceptibilityGenome analysisHumanHuman cellMajor clinical studyMicrosatellite markerPriority journalReverse transcription polymerase chain reactionRNA hybridizationSalivary glandSignal transductionSjoegren syndromeAdultAgedAllelesEpithelial CellsFemaleGene ExpressionGene Expression ProfilingGene FrequencyGenetic Predisposition to DiseaseGenome-Wide Association StudyGenotypeHumansMicrosatellite RepeatsMiddle AgedSalivary GlandsSjogren's SyndromeApoptosisCdna microarrayEpithelial cellsGenome-wide association studyInterferonSjögren's syndromeHumanChromosomesGene expression and chromosomal location for susceptibility to Sjögren's syndromearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Pérez, PaolaAnaya, Juan-ManuelAguilera, SergioUrzúa, UlisesMunroe, DavidMolina, ClaudioHermoso, Marcela A.Cherry, James MichaelAlliende, CeciliaOlea, NancyRuiz-Narváez, EdwardGonzález, María-JulietaORIGINAL1-s2-0-S0896841109000699-main.pdfapplication/pdf654685https://repository.urosario.edu.co/bitstreams/2365a0c2-29db-49a9-a817-48e75066b5bd/download9d4d677fbfc390dcbbe6a7c0391b6677MD51TEXT1-s2-0-S0896841109000699-main.pdf.txt1-s2-0-S0896841109000699-main.pdf.txtExtracted texttext/plain55495https://repository.urosario.edu.co/bitstreams/6b61707b-efc7-4e8e-a08e-3c9f2f958138/downloadf246e3bec4eade5b9dc4e35e0ba24289MD52THUMBNAIL1-s2-0-S0896841109000699-main.pdf.jpg1-s2-0-S0896841109000699-main.pdf.jpgGenerated Thumbnailimage/jpeg4749https://repository.urosario.edu.co/bitstreams/d64d8d57-a97a-4fbb-955c-0b65724bb69b/download4164fd9416c3bb43a4d9d76dc52317aaMD5310336/22251oai:repository.urosario.edu.co:10336/222512022-05-02 07:37:13.590136https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co