Gene expression and chromosomal location for susceptibility to Sjögren's syndrome
Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling i...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2009
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/22251
- Acceso en línea:
- https://doi.org/10.1016/j.jaut.2009.05.001
https://repository.urosario.edu.co/handle/10336/22251
- Palabra clave:
- Complementary DNA
Genomic DNA
Interferon
RNA
Adult
Aged
Apoptosis
Article
Chromosomal localization
Controlled study
DNA microarray
Epithelium cell
Female
Gene amplification
Gene expression profiling
Gene expression regulation
Gene locus
Gene overexpression
Genetic susceptibility
Genome analysis
Human
Human cell
Major clinical study
Microsatellite marker
Priority journal
Reverse transcription polymerase chain reaction
RNA hybridization
Salivary gland
Signal transduction
Sjoegren syndrome
Adult
Aged
Alleles
Epithelial Cells
Female
Gene Expression
Gene Expression Profiling
Gene Frequency
Genetic Predisposition to Disease
Genome-Wide Association Study
Genotype
Humans
Microsatellite Repeats
Middle Aged
Salivary Glands
Sjogren's Syndrome
Apoptosis
Cdna microarray
Epithelial cells
Genome-wide association study
Interferon
Sjögren's syndrome
Human
Chromosomes
- Rights
- License
- Abierto (Texto Completo)
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|
dc.title.spa.fl_str_mv |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
title |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
spellingShingle |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome Complementary DNA Genomic DNA Interferon RNA Adult Aged Apoptosis Article Chromosomal localization Controlled study DNA microarray Epithelium cell Female Gene amplification Gene expression profiling Gene expression regulation Gene locus Gene overexpression Genetic susceptibility Genome analysis Human Human cell Major clinical study Microsatellite marker Priority journal Reverse transcription polymerase chain reaction RNA hybridization Salivary gland Signal transduction Sjoegren syndrome Adult Aged Alleles Epithelial Cells Female Gene Expression Gene Expression Profiling Gene Frequency Genetic Predisposition to Disease Genome-Wide Association Study Genotype Humans Microsatellite Repeats Middle Aged Salivary Glands Sjogren's Syndrome Apoptosis Cdna microarray Epithelial cells Genome-wide association study Interferon Sjögren's syndrome Human Chromosomes |
title_short |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
title_full |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
title_fullStr |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
title_full_unstemmed |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
title_sort |
Gene expression and chromosomal location for susceptibility to Sjögren's syndrome |
dc.subject.keyword.spa.fl_str_mv |
Complementary DNA Genomic DNA Interferon RNA Adult Aged Apoptosis Article Chromosomal localization Controlled study DNA microarray Epithelium cell Female Gene amplification Gene expression profiling Gene expression regulation Gene locus Gene overexpression Genetic susceptibility Genome analysis Human Human cell Major clinical study Microsatellite marker Priority journal Reverse transcription polymerase chain reaction RNA hybridization Salivary gland Signal transduction Sjoegren syndrome Adult Aged Alleles Epithelial Cells Female Gene Expression Gene Expression Profiling Gene Frequency Genetic Predisposition to Disease Genome-Wide Association Study Genotype Humans Microsatellite Repeats Middle Aged Salivary Glands Sjogren's Syndrome Apoptosis Cdna microarray Epithelial cells Genome-wide association study Interferon Sjögren's syndrome |
topic |
Complementary DNA Genomic DNA Interferon RNA Adult Aged Apoptosis Article Chromosomal localization Controlled study DNA microarray Epithelium cell Female Gene amplification Gene expression profiling Gene expression regulation Gene locus Gene overexpression Genetic susceptibility Genome analysis Human Human cell Major clinical study Microsatellite marker Priority journal Reverse transcription polymerase chain reaction RNA hybridization Salivary gland Signal transduction Sjoegren syndrome Adult Aged Alleles Epithelial Cells Female Gene Expression Gene Expression Profiling Gene Frequency Genetic Predisposition to Disease Genome-Wide Association Study Genotype Humans Microsatellite Repeats Middle Aged Salivary Glands Sjogren's Syndrome Apoptosis Cdna microarray Epithelial cells Genome-wide association study Interferon Sjögren's syndrome Human Chromosomes |
dc.subject.keyword.eng.fl_str_mv |
Human Chromosomes |
description |
Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling in fractions enriched in epithelial cells from labial salivary glands (LSGs) of patients with primary SS and identify chromosomal regions harboring susceptibility genes expressed in epithelial cells. A combined approach of gene expression and genome-wide association study was used. Enriched epithelial cell fractions were obtained from LSGs of patients and controls. Amplified total RNA was labeled and hybridized to 10K cDNA microarrays. Results were normalized and subjected to statistical and functional analysis. A genome-wide microsatellite screen at 10 cM resolution (393 markers) was performed. In salivary gland-epithelial cells from patients 528 genes were expressed differentially in comparison to controls. Pathways not previously linked to disease were found to be altered. Twenty-eight and 15 genes associated with apoptosis were up-regulated and down regulated, respectively. Interferon-related genes, most of which participated in interferon signaling, were also found to be up-regulated. From the genome-wide screen, 6 markers showed evidence of highly significant association with the disease. Of these, five loci harbor genes differentially expressed in patients LSG-epithelial cells. Our results show that in enriched gland-epithelial cells of pSS, both pro-apoptotic/anti-apoptotic and interferon signaling inhibition/stimulation balances may occur. Genes found over-expressed in epithelial cells are candidates for disease susceptibility. © 2009 Elsevier Ltd. All rights reserved. |
publishDate |
2009 |
dc.date.created.spa.fl_str_mv |
2009 |
dc.date.accessioned.none.fl_str_mv |
2020-05-25T23:55:53Z |
dc.date.available.none.fl_str_mv |
2020-05-25T23:55:53Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.jaut.2009.05.001 |
dc.identifier.issn.none.fl_str_mv |
10959157 08968411 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/22251 |
url |
https://doi.org/10.1016/j.jaut.2009.05.001 https://repository.urosario.edu.co/handle/10336/22251 |
identifier_str_mv |
10959157 08968411 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
108 |
dc.relation.citationIssue.none.fl_str_mv |
No. 2 |
dc.relation.citationStartPage.none.fl_str_mv |
99 |
dc.relation.citationTitle.none.fl_str_mv |
Journal of Autoimmunity |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 33 |
dc.relation.ispartof.spa.fl_str_mv |
Journal of Autoimmunity, ISSN:10959157, 08968411, Vol.33, No.2 (2009); pp. 99-108 |
dc.relation.uri.spa.fl_str_mv |
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dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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reponame:Repositorio Institucional EdocUR |
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fd7acce8-5400-4f35-85d5-0ceb85e062b719474778600da634d6f-b330-4658-b68f-577d2d50aab91b9bfe95-287d-4265-804c-6b6208a0143998037345-9c84-497e-89fe-829e8e0219f5fe958c95-471d-4081-bfc2-72346f89e56d2e6809ce-182a-4cde-9e6e-14dc7f3c27d9fb66c393-0261-47e3-b133-7215a6765bcf1edea94f-bf14-4f04-8374-ee09955af8e45f45f8d5-2c4c-4387-9501-2a7d6720cc42a94a1783-6ef4-4e90-8ff0-83ffa188cd6390d0f62a-6282-4eab-9658-bf09d3a6e0452020-05-25T23:55:53Z2020-05-25T23:55:53Z2009Primary Sjögren's syndrome (SS) is a chronic inflammatory autoimmune disease affecting mainly the exocrine glands. Its physio-pathology is poorly understood and most of the knowledge has been related to the inflammatory component. The aim of this work was to evaluate gene expression profiling in fractions enriched in epithelial cells from labial salivary glands (LSGs) of patients with primary SS and identify chromosomal regions harboring susceptibility genes expressed in epithelial cells. A combined approach of gene expression and genome-wide association study was used. Enriched epithelial cell fractions were obtained from LSGs of patients and controls. Amplified total RNA was labeled and hybridized to 10K cDNA microarrays. Results were normalized and subjected to statistical and functional analysis. A genome-wide microsatellite screen at 10 cM resolution (393 markers) was performed. In salivary gland-epithelial cells from patients 528 genes were expressed differentially in comparison to controls. Pathways not previously linked to disease were found to be altered. Twenty-eight and 15 genes associated with apoptosis were up-regulated and down regulated, respectively. Interferon-related genes, most of which participated in interferon signaling, were also found to be up-regulated. From the genome-wide screen, 6 markers showed evidence of highly significant association with the disease. Of these, five loci harbor genes differentially expressed in patients LSG-epithelial cells. Our results show that in enriched gland-epithelial cells of pSS, both pro-apoptotic/anti-apoptotic and interferon signaling inhibition/stimulation balances may occur. Genes found over-expressed in epithelial cells are candidates for disease susceptibility. © 2009 Elsevier Ltd. All rights reserved.application/pdfhttps://doi.org/10.1016/j.jaut.2009.05.0011095915708968411https://repository.urosario.edu.co/handle/10336/22251eng108No. 299Journal of AutoimmunityVol. 33Journal of Autoimmunity, ISSN:10959157, 08968411, Vol.33, No.2 (2009); pp. 99-108https://www.scopus.com/inward/record.uri?eid=2-s2.0-68249087935&doi=10.1016%2fj.jaut.2009.05.001&partnerID=40&md5=2e474b671c82dc5adc055d850fd33f2eAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementary DNAGenomic DNAInterferonRNAAdultAgedApoptosisArticleChromosomal localizationControlled studyDNA microarrayEpithelium cellFemaleGene amplificationGene expression profilingGene expression regulationGene locusGene overexpressionGenetic susceptibilityGenome analysisHumanHuman cellMajor clinical studyMicrosatellite markerPriority journalReverse transcription polymerase chain reactionRNA hybridizationSalivary glandSignal transductionSjoegren syndromeAdultAgedAllelesEpithelial CellsFemaleGene ExpressionGene Expression ProfilingGene FrequencyGenetic Predisposition to DiseaseGenome-Wide Association StudyGenotypeHumansMicrosatellite RepeatsMiddle AgedSalivary GlandsSjogren's SyndromeApoptosisCdna microarrayEpithelial cellsGenome-wide association studyInterferonSjögren's syndromeHumanChromosomesGene expression and chromosomal location for susceptibility to Sjögren's syndromearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Pérez, PaolaAnaya, Juan-ManuelAguilera, SergioUrzúa, UlisesMunroe, DavidMolina, ClaudioHermoso, Marcela A.Cherry, James MichaelAlliende, CeciliaOlea, NancyRuiz-Narváez, EdwardGonzález, María-JulietaORIGINAL1-s2-0-S0896841109000699-main.pdfapplication/pdf654685https://repository.urosario.edu.co/bitstreams/2365a0c2-29db-49a9-a817-48e75066b5bd/download9d4d677fbfc390dcbbe6a7c0391b6677MD51TEXT1-s2-0-S0896841109000699-main.pdf.txt1-s2-0-S0896841109000699-main.pdf.txtExtracted texttext/plain55495https://repository.urosario.edu.co/bitstreams/6b61707b-efc7-4e8e-a08e-3c9f2f958138/downloadf246e3bec4eade5b9dc4e35e0ba24289MD52THUMBNAIL1-s2-0-S0896841109000699-main.pdf.jpg1-s2-0-S0896841109000699-main.pdf.jpgGenerated Thumbnailimage/jpeg4749https://repository.urosario.edu.co/bitstreams/d64d8d57-a97a-4fbb-955c-0b65724bb69b/download4164fd9416c3bb43a4d9d76dc52317aaMD5310336/22251oai:repository.urosario.edu.co:10336/222512022-05-02 07:37:13.590136https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |