Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni

Comamonas testosteroni is a soil bacterium, which can use a variety of steroids as carbon and energy source. Even if it can be estimated that the complete degradation of the steroid nucleus requires more than 20 enzymatic reactions, the complete molecular characterization of the genes encoding these...

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Tipo de recurso:
Fecha de publicación:
2004
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27836
Acceso en línea:
https://doi.org/10.1016/j.jsbmb.2003.10.010
https://repository.urosario.edu.co/handle/10336/27836
Palabra clave:
Steroid
Degradation
Comamonas testosteroni
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License
Restringido (Acceso a grupos específicos)
id EDOCUR2_fa86cc0565c4633fdf3d221fc87d8401
oai_identifier_str oai:repository.urosario.edu.co:10336/27836
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 3d9fe2dc-09b9-4a1e-a259-6474dae0b120791423246004ac95538-d112-449f-8924-7b77205ac18490ddb4af-e3ec-4770-aab0-f201b0ae97ba2020-08-19T14:44:09Z2020-08-19T14:44:09Z2004-01-01Comamonas testosteroni is a soil bacterium, which can use a variety of steroids as carbon and energy source. Even if it can be estimated that the complete degradation of the steroid nucleus requires more than 20 enzymatic reactions, the complete molecular characterization of the genes encoding these steroid degradative enzymes as well as the genetic organization of them remain to be elucidated. We have previously reported the cloning and nucleotide sequence of two steroid-inducible genes, ?hsd and stdC encoding 3?-17?-hydroxysteroid dehydrogenase and a hypothetical protein respectively, located in both ends of a 3.2 kb HindIII fragment. Herein, we report the cloning and characterization of another steroid-inducible gene, called sip48 (steroid inducible protein), located between these two genes. The analysis of Sip48 amino acid sequence predicts a protein of 438 amino acids with a molecular mass of 48.5 kDa. This protein bears high homology with conserved hypothetical proteins of unknown function described in Pseudomonas aeruginosa, Pseudomonas syringae, Pseudomonas putida, Burkholderia fungorum, Shewanella oneidensis, Pseudomonas fluorescens and Thauera aromatica. The predicted protein shows a typical structure of a leader peptide at its N-terminus. A 48.5 kDa protein encoded by the recombinant plasmid was detected by SDS–PAGE analysis of in vitro []-methionine labeled polypeptides. Analysis of gene expression indicates that Sip48 is tightly controlled at the transcriptional level by several steroid compounds. In addition, transcriptional analysis of sip48 and ?hsd in a sip48 mutant strain, indicates that both genes are transcribed as a polycistronic mRNA. lacZ transcriptional fusions integrated into the chromosome of C. testosteroni demonstrate that a steroid-inducible promoter located upstream of sip48 regulates the expression of both genes.application/pdfhttps://doi.org/10.1016/j.jsbmb.2003.10.010ISSN: 0960-0760EISSN: 1879-1220https://repository.urosario.edu.co/handle/10336/27836engElsevier100No. 191Journal of Steroid Biochemistry and Molecular BiologyVol. 88Journal of Steroid Biochemistry and Molecular Biology, ISSN: 0960-0760;EISSN: 1879-1220, Vol.88, No.1 (2004); pp. 91-100https://www.sciencedirect.com/science/article/abs/pii/S0960076004000020Restringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecJournal of Steroid Biochemistry and Molecular Biologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURSteroidDegradationComamonas testosteroniIdentification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroniIdentificación de un nuevo gen inducible por esteroides asociado con el locus ?hsd de Comamonas testosteroniarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Pruneda-Paz, José LuisLinares, MauricioCabrera, Julio E.Genti-Raimondi, Susana10336/27836oai:repository.urosario.edu.co:10336/278362021-09-07 16:19:34.731https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
dc.title.TranslatedTitle.spa.fl_str_mv Identificación de un nuevo gen inducible por esteroides asociado con el locus ?hsd de Comamonas testosteroni
title Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
spellingShingle Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
Steroid
Degradation
Comamonas testosteroni
title_short Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
title_full Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
title_fullStr Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
title_full_unstemmed Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
title_sort Identification of a novel steroid inducible gene associated with the ?hsd locus of Comamonas testosteroni
dc.subject.keyword.spa.fl_str_mv Steroid
Degradation
Comamonas testosteroni
topic Steroid
Degradation
Comamonas testosteroni
description Comamonas testosteroni is a soil bacterium, which can use a variety of steroids as carbon and energy source. Even if it can be estimated that the complete degradation of the steroid nucleus requires more than 20 enzymatic reactions, the complete molecular characterization of the genes encoding these steroid degradative enzymes as well as the genetic organization of them remain to be elucidated. We have previously reported the cloning and nucleotide sequence of two steroid-inducible genes, ?hsd and stdC encoding 3?-17?-hydroxysteroid dehydrogenase and a hypothetical protein respectively, located in both ends of a 3.2 kb HindIII fragment. Herein, we report the cloning and characterization of another steroid-inducible gene, called sip48 (steroid inducible protein), located between these two genes. The analysis of Sip48 amino acid sequence predicts a protein of 438 amino acids with a molecular mass of 48.5 kDa. This protein bears high homology with conserved hypothetical proteins of unknown function described in Pseudomonas aeruginosa, Pseudomonas syringae, Pseudomonas putida, Burkholderia fungorum, Shewanella oneidensis, Pseudomonas fluorescens and Thauera aromatica. The predicted protein shows a typical structure of a leader peptide at its N-terminus. A 48.5 kDa protein encoded by the recombinant plasmid was detected by SDS–PAGE analysis of in vitro []-methionine labeled polypeptides. Analysis of gene expression indicates that Sip48 is tightly controlled at the transcriptional level by several steroid compounds. In addition, transcriptional analysis of sip48 and ?hsd in a sip48 mutant strain, indicates that both genes are transcribed as a polycistronic mRNA. lacZ transcriptional fusions integrated into the chromosome of C. testosteroni demonstrate that a steroid-inducible promoter located upstream of sip48 regulates the expression of both genes.
publishDate 2004
dc.date.created.spa.fl_str_mv 2004-01-01
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:44:09Z
dc.date.available.none.fl_str_mv 2020-08-19T14:44:09Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.jsbmb.2003.10.010
dc.identifier.issn.none.fl_str_mv ISSN: 0960-0760
EISSN: 1879-1220
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27836
url https://doi.org/10.1016/j.jsbmb.2003.10.010
https://repository.urosario.edu.co/handle/10336/27836
identifier_str_mv ISSN: 0960-0760
EISSN: 1879-1220
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 100
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 91
dc.relation.citationTitle.none.fl_str_mv Journal of Steroid Biochemistry and Molecular Biology
dc.relation.citationVolume.none.fl_str_mv Vol. 88
dc.relation.ispartof.spa.fl_str_mv Journal of Steroid Biochemistry and Molecular Biology, ISSN: 0960-0760;EISSN: 1879-1220, Vol.88, No.1 (2004); pp. 91-100
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S0960076004000020
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier
dc.source.spa.fl_str_mv Journal of Steroid Biochemistry and Molecular Biology
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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