PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis

Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restrict...

Full description

Autores:
Tipo de recurso:
Fecha de publicación:
2017
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/21384
Acceso en línea:
https://doi.org/10.1186/s13071-017-2183-8
https://repository.urosario.edu.co/handle/10336/21384
Palabra clave:
Proteína adhesiva
Plasmodium vivax
Diversidad genética
Región funcional conservada
Actividad de unión a reticulocitos
Enfermedades
Adhesin protein
Plasmodium vivax
Genetic diversity
Conserved functional region
Reticulocyte binding activity
Plasmodium vivax
Reticulositos
Malaria
Rights
License
Abierto (Texto Completo)
id EDOCUR2_f61af7ba30a413fe12f9abd8110fb9cd
oai_identifier_str oai:repository.urosario.edu.co:10336/21384
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 239d2979-65a3-4034-a863-5fb3963efdf4600bcdffe09-aaac-461a-8ffe-634dc2e94abd60021198e38-6096-4bf4-b5a1-3f7851169cd56007f799a76-52d4-4b25-ac8a-2cd27a9a2ef5600e061f6dd-d149-4281-b11b-376cdc027508600796530656002020-04-01T22:28:57Z2020-04-01T22:28:57Z20172017Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells. © 2017 The Author(s).application/pdfhttps://doi.org/10.1186/s13071-017-2183-81756-3305https://repository.urosario.edu.co/handle/10336/21384engNo. 1Parasites and VectorsVol. 10Parasites and Vectors, ISSN: 1756-3305 Vol. 10, No. 1 (2017)https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-017-2183-8Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURProteína adhesivaPlasmodium vivaxDiversidad genéticaRegión funcional conservadaActividad de unión a reticulocitosEnfermedades616600Adhesin proteinPlasmodium vivaxGenetic diversityConserved functional regionReticulocyte binding activityPlasmodium vivaxReticulositosMalariaPvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysisarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Baquero, Luis A.Moreno-Perez, Darwin AndresGarzón-Ospina, DiegoForero Rodríguez, JohannaOrtiz-Suárez, Heidy D.Patarroyo, Manuel A.Baquero, Luis AlbertoMoreno-Pérez, Darwin A.Garzón-Ospina, DiegoForero-Rodríguez, JohannaOrtiz-Suárez, Heidy D.Patarroyo, Manuel A.ORIGINALPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdfapplication/pdf1060499https://repository.urosario.edu.co/bitstreams/f4343ba5-05cf-4d90-98ff-57a8448a8008/downloadf475d50e6ffda03d45ad02ce94b33578MD51TEXTPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.txtPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.txtExtracted texttext/plain57538https://repository.urosario.edu.co/bitstreams/377ef190-e0cc-45fd-85f1-bac6a17c8395/download7c626cb032e33caec4bbd11419dc1287MD52THUMBNAILPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.jpgPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.jpgGenerated Thumbnailimage/jpeg4633https://repository.urosario.edu.co/bitstreams/5bb9f7bd-fdfa-40f4-a679-ea4aba364eb7/download952651f06caaeba508c25708edc224c0MD5310336/21384oai:repository.urosario.edu.co:10336/213842020-05-13 14:48:08.922https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
title PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
spellingShingle PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
Proteína adhesiva
Plasmodium vivax
Diversidad genética
Región funcional conservada
Actividad de unión a reticulocitos
Enfermedades
Adhesin protein
Plasmodium vivax
Genetic diversity
Conserved functional region
Reticulocyte binding activity
Plasmodium vivax
Reticulositos
Malaria
title_short PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
title_full PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
title_fullStr PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
title_full_unstemmed PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
title_sort PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
dc.subject.spa.fl_str_mv Proteína adhesiva
Plasmodium vivax
Diversidad genética
Región funcional conservada
Actividad de unión a reticulocitos
topic Proteína adhesiva
Plasmodium vivax
Diversidad genética
Región funcional conservada
Actividad de unión a reticulocitos
Enfermedades
Adhesin protein
Plasmodium vivax
Genetic diversity
Conserved functional region
Reticulocyte binding activity
Plasmodium vivax
Reticulositos
Malaria
dc.subject.ddc.spa.fl_str_mv Enfermedades
dc.subject.keyword.spa.fl_str_mv Adhesin protein
Plasmodium vivax
Genetic diversity
Conserved functional region
Reticulocyte binding activity
dc.subject.lemb.spa.fl_str_mv Plasmodium vivax
Reticulositos
Malaria
description Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells. © 2017 The Author(s).
publishDate 2017
dc.date.created.none.fl_str_mv 2017
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2020-04-01T22:28:57Z
dc.date.available.none.fl_str_mv 2020-04-01T22:28:57Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1186/s13071-017-2183-8
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/21384
url https://doi.org/10.1186/s13071-017-2183-8
https://repository.urosario.edu.co/handle/10336/21384
identifier_str_mv 1756-3305
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationTitle.none.fl_str_mv Parasites and Vectors
dc.relation.citationVolume.none.fl_str_mv Vol. 10
dc.relation.ispartof.spa.fl_str_mv Parasites and Vectors, ISSN: 1756-3305 Vol. 10, No. 1 (2017)
dc.relation.uri.spa.fl_str_mv https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-017-2183-8
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
bitstream.url.fl_str_mv https://repository.urosario.edu.co/bitstreams/f4343ba5-05cf-4d90-98ff-57a8448a8008/download
https://repository.urosario.edu.co/bitstreams/377ef190-e0cc-45fd-85f1-bac6a17c8395/download
https://repository.urosario.edu.co/bitstreams/5bb9f7bd-fdfa-40f4-a679-ea4aba364eb7/download
bitstream.checksum.fl_str_mv f475d50e6ffda03d45ad02ce94b33578
7c626cb032e33caec4bbd11419dc1287
952651f06caaeba508c25708edc224c0
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167543669063680