PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis
Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restrict...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2017
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/21384
- Acceso en línea:
- https://doi.org/10.1186/s13071-017-2183-8
https://repository.urosario.edu.co/handle/10336/21384
- Palabra clave:
- Proteína adhesiva
Plasmodium vivax
Diversidad genética
Región funcional conservada
Actividad de unión a reticulocitos
Enfermedades
Adhesin protein
Plasmodium vivax
Genetic diversity
Conserved functional region
Reticulocyte binding activity
Plasmodium vivax
Reticulositos
Malaria
- Rights
- License
- Abierto (Texto Completo)
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239d2979-65a3-4034-a863-5fb3963efdf4600bcdffe09-aaac-461a-8ffe-634dc2e94abd60021198e38-6096-4bf4-b5a1-3f7851169cd56007f799a76-52d4-4b25-ac8a-2cd27a9a2ef5600e061f6dd-d149-4281-b11b-376cdc027508600796530656002020-04-01T22:28:57Z2020-04-01T22:28:57Z20172017Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells. © 2017 The Author(s).application/pdfhttps://doi.org/10.1186/s13071-017-2183-81756-3305https://repository.urosario.edu.co/handle/10336/21384engNo. 1Parasites and VectorsVol. 10Parasites and Vectors, ISSN: 1756-3305 Vol. 10, No. 1 (2017)https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-017-2183-8Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURProteína adhesivaPlasmodium vivaxDiversidad genéticaRegión funcional conservadaActividad de unión a reticulocitosEnfermedades616600Adhesin proteinPlasmodium vivaxGenetic diversityConserved functional regionReticulocyte binding activityPlasmodium vivaxReticulositosMalariaPvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysisarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Baquero, Luis A.Moreno-Perez, Darwin AndresGarzón-Ospina, DiegoForero Rodríguez, JohannaOrtiz-Suárez, Heidy D.Patarroyo, Manuel A.Baquero, Luis AlbertoMoreno-Pérez, Darwin A.Garzón-Ospina, DiegoForero-Rodríguez, JohannaOrtiz-Suárez, Heidy D.Patarroyo, Manuel A.ORIGINALPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdfapplication/pdf1060499https://repository.urosario.edu.co/bitstreams/f4343ba5-05cf-4d90-98ff-57a8448a8008/downloadf475d50e6ffda03d45ad02ce94b33578MD51TEXTPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.txtPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.txtExtracted texttext/plain57538https://repository.urosario.edu.co/bitstreams/377ef190-e0cc-45fd-85f1-bac6a17c8395/download7c626cb032e33caec4bbd11419dc1287MD52THUMBNAILPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.jpgPvGAMA_reticulocyte_binding_activity_predicting_conserved_functional_regions_by_natural_selection_analysis.pdf.jpgGenerated Thumbnailimage/jpeg4633https://repository.urosario.edu.co/bitstreams/5bb9f7bd-fdfa-40f4-a679-ea4aba364eb7/download952651f06caaeba508c25708edc224c0MD5310336/21384oai:repository.urosario.edu.co:10336/213842020-05-13 14:48:08.922https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
title |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
spellingShingle |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis Proteína adhesiva Plasmodium vivax Diversidad genética Región funcional conservada Actividad de unión a reticulocitos Enfermedades Adhesin protein Plasmodium vivax Genetic diversity Conserved functional region Reticulocyte binding activity Plasmodium vivax Reticulositos Malaria |
title_short |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
title_full |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
title_fullStr |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
title_full_unstemmed |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
title_sort |
PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis |
dc.subject.spa.fl_str_mv |
Proteína adhesiva Plasmodium vivax Diversidad genética Región funcional conservada Actividad de unión a reticulocitos |
topic |
Proteína adhesiva Plasmodium vivax Diversidad genética Región funcional conservada Actividad de unión a reticulocitos Enfermedades Adhesin protein Plasmodium vivax Genetic diversity Conserved functional region Reticulocyte binding activity Plasmodium vivax Reticulositos Malaria |
dc.subject.ddc.spa.fl_str_mv |
Enfermedades |
dc.subject.keyword.spa.fl_str_mv |
Adhesin protein Plasmodium vivax Genetic diversity Conserved functional region Reticulocyte binding activity |
dc.subject.lemb.spa.fl_str_mv |
Plasmodium vivax Reticulositos Malaria |
description |
Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells. © 2017 The Author(s). |
publishDate |
2017 |
dc.date.created.none.fl_str_mv |
2017 |
dc.date.issued.none.fl_str_mv |
2017 |
dc.date.accessioned.none.fl_str_mv |
2020-04-01T22:28:57Z |
dc.date.available.none.fl_str_mv |
2020-04-01T22:28:57Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1186/s13071-017-2183-8 |
dc.identifier.issn.none.fl_str_mv |
1756-3305 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/21384 |
url |
https://doi.org/10.1186/s13071-017-2183-8 https://repository.urosario.edu.co/handle/10336/21384 |
identifier_str_mv |
1756-3305 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationTitle.none.fl_str_mv |
Parasites and Vectors |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 10 |
dc.relation.ispartof.spa.fl_str_mv |
Parasites and Vectors, ISSN: 1756-3305 Vol. 10, No. 1 (2017) |
dc.relation.uri.spa.fl_str_mv |
https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-017-2183-8 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
institution |
Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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