Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
The process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investi...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2005
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/26011
- Acceso en línea:
- https://doi.org/10.1016/j.bbrc.2005.05.018
https://repository.urosario.edu.co/handle/10336/26011
- Palabra clave:
- Rv1510
Mycobacterium tuberculosis
Tuberculosis
Peptides
Binding
- Rights
- License
- Restringido (Acceso a grupos específicos)
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0ad382b4-d6b1-4878-a4a1-bd78fc45e107-14272869d-a644-44e9-a7a4-9c26d936bb9e-1bbc66b15-6431-42e4-ae1d-915ec7a26072-1ef6547b6-3130-435a-8a09-7ca050cd7b64-1f2d05f42-2948-4c51-8389-8c3817c2d0f1-1a650efd0-af5f-4ce9-9620-e1cad93a9173-191225589-1d191987c-6e13-4f7f-88bd-c34b437b85c6-151721018-17b000441-0f07-4b3f-8025-f0c59b37d338-1e5b1a105-1b59-4796-a953-f0ddede3d0de-1e52df738-07b5-4f26-8e9d-a2e59871647a-179653065-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-1518488266002020-08-06T16:20:27Z2020-08-06T16:20:27Z2005-07-08The process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investigated by analysing the M. tuberculosis genome sequence data reported by Cole et al. and a previous report that used PCR assays to show that the Rv1510 gene was only present in M. tuberculosis. This article confirmed all the above and identified the transcribed gene in M. tuberculosis, Mycobacterium africanum, and in M. tuberculosis clinical isolates. Antibodies raised against peptides from this protein recognised a 44 kDa band, corresponding to Rv1510c theoretical mass (44,294 Da). Assays involving synthetic peptides covering the whole protein binding to U937 and A549 cell lines led to recognising five high activity binding peptides in the Rv1510 protein: 11094, 11095, 11105, 11108, and 11111. Their affinity constants and Hill coefficients were determined by using U937 cells. Cross-linking assays performed with some of these HABPs showed that they specifically bound to a U937 cell line 51 kDa protein, but not to Hep G2 or red blood cell proteins, showing this interaction’s specificity.application/pdfhttps://doi.org/10.1016/j.bbrc.2005.05.018ISSN: 0006-291XEISSN: 1090-2104https://repository.urosario.edu.co/handle/10336/26011engElsevier781No. 3771Biochemical and Biophysical Research CommunicationsVol. 332Biochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.332, No.3 (2005); pp.771-781https://www.sciencedirect.com/science/article/abs/pii/S0006291X05009642Restringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecBiochemical and Biophysical Research Communicationsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURRv1510Mycobacterium tuberculosisTuberculosisPeptidesBindingCharacterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell linesCaracterizando la proteína Mycobacterium tuberculosis Rv1510c y determinando sus secuencias que se unen específicamente a dos líneas celulares dianaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Vera-Bravo, RicardoTorres, ElizabethValbuena, John J.Rodríguez, Luis E.Puentes, ÁlvaroGarcía, Javier E.Curtidor, HernandoCortés, JimenaVanegas, MagnoliaRivera, Zuly J.Díaz, AndreaCalderon, Martha N.Patarroyo, Manuel A.Patarroyo, Manuel E.Ocampo, Marisol10336/26011oai:repository.urosario.edu.co:10336/260112022-05-02 07:37:21.807888https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
dc.title.TranslatedTitle.spa.fl_str_mv |
Caracterizando la proteína Mycobacterium tuberculosis Rv1510c y determinando sus secuencias que se unen específicamente a dos líneas celulares diana |
title |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
spellingShingle |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines Rv1510 Mycobacterium tuberculosis Tuberculosis Peptides Binding |
title_short |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
title_full |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
title_fullStr |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
title_full_unstemmed |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
title_sort |
Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines |
dc.subject.keyword.spa.fl_str_mv |
Rv1510 Mycobacterium tuberculosis Tuberculosis Peptides Binding |
topic |
Rv1510 Mycobacterium tuberculosis Tuberculosis Peptides Binding |
description |
The process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investigated by analysing the M. tuberculosis genome sequence data reported by Cole et al. and a previous report that used PCR assays to show that the Rv1510 gene was only present in M. tuberculosis. This article confirmed all the above and identified the transcribed gene in M. tuberculosis, Mycobacterium africanum, and in M. tuberculosis clinical isolates. Antibodies raised against peptides from this protein recognised a 44 kDa band, corresponding to Rv1510c theoretical mass (44,294 Da). Assays involving synthetic peptides covering the whole protein binding to U937 and A549 cell lines led to recognising five high activity binding peptides in the Rv1510 protein: 11094, 11095, 11105, 11108, and 11111. Their affinity constants and Hill coefficients were determined by using U937 cells. Cross-linking assays performed with some of these HABPs showed that they specifically bound to a U937 cell line 51 kDa protein, but not to Hep G2 or red blood cell proteins, showing this interaction’s specificity. |
publishDate |
2005 |
dc.date.created.spa.fl_str_mv |
2005-07-08 |
dc.date.accessioned.none.fl_str_mv |
2020-08-06T16:20:27Z |
dc.date.available.none.fl_str_mv |
2020-08-06T16:20:27Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.bbrc.2005.05.018 |
dc.identifier.issn.none.fl_str_mv |
ISSN: 0006-291X EISSN: 1090-2104 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/26011 |
url |
https://doi.org/10.1016/j.bbrc.2005.05.018 https://repository.urosario.edu.co/handle/10336/26011 |
identifier_str_mv |
ISSN: 0006-291X EISSN: 1090-2104 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
781 |
dc.relation.citationIssue.none.fl_str_mv |
No. 3 |
dc.relation.citationStartPage.none.fl_str_mv |
771 |
dc.relation.citationTitle.none.fl_str_mv |
Biochemical and Biophysical Research Communications |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 332 |
dc.relation.ispartof.spa.fl_str_mv |
Biochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.332, No.3 (2005); pp.771-781 |
dc.relation.uri.spa.fl_str_mv |
https://www.sciencedirect.com/science/article/abs/pii/S0006291X05009642 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_16ec |
dc.rights.acceso.spa.fl_str_mv |
Restringido (Acceso a grupos específicos) |
rights_invalid_str_mv |
Restringido (Acceso a grupos específicos) http://purl.org/coar/access_right/c_16ec |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier |
dc.source.spa.fl_str_mv |
Biochemical and Biophysical Research Communications |
institution |
Universidad del Rosario |
dc.source.instname.none.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167507504726017 |