Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines

The process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investi...

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Autores:
Tipo de recurso:
Fecha de publicación:
2005
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26011
Acceso en línea:
https://doi.org/10.1016/j.bbrc.2005.05.018
https://repository.urosario.edu.co/handle/10336/26011
Palabra clave:
Rv1510
Mycobacterium tuberculosis
Tuberculosis
Peptides
Binding
Rights
License
Restringido (Acceso a grupos específicos)
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network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 0ad382b4-d6b1-4878-a4a1-bd78fc45e107-14272869d-a644-44e9-a7a4-9c26d936bb9e-1bbc66b15-6431-42e4-ae1d-915ec7a26072-1ef6547b6-3130-435a-8a09-7ca050cd7b64-1f2d05f42-2948-4c51-8389-8c3817c2d0f1-1a650efd0-af5f-4ce9-9620-e1cad93a9173-191225589-1d191987c-6e13-4f7f-88bd-c34b437b85c6-151721018-17b000441-0f07-4b3f-8025-f0c59b37d338-1e5b1a105-1b59-4796-a953-f0ddede3d0de-1e52df738-07b5-4f26-8e9d-a2e59871647a-179653065-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-1518488266002020-08-06T16:20:27Z2020-08-06T16:20:27Z2005-07-08The process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investigated by analysing the M. tuberculosis genome sequence data reported by Cole et al. and a previous report that used PCR assays to show that the Rv1510 gene was only present in M. tuberculosis. This article confirmed all the above and identified the transcribed gene in M. tuberculosis, Mycobacterium africanum, and in M. tuberculosis clinical isolates. Antibodies raised against peptides from this protein recognised a 44 kDa band, corresponding to Rv1510c theoretical mass (44,294 Da). Assays involving synthetic peptides covering the whole protein binding to U937 and A549 cell lines led to recognising five high activity binding peptides in the Rv1510 protein: 11094, 11095, 11105, 11108, and 11111. Their affinity constants and Hill coefficients were determined by using U937 cells. Cross-linking assays performed with some of these HABPs showed that they specifically bound to a U937 cell line 51 kDa protein, but not to Hep G2 or red blood cell proteins, showing this interaction’s specificity.application/pdfhttps://doi.org/10.1016/j.bbrc.2005.05.018ISSN: 0006-291XEISSN: 1090-2104https://repository.urosario.edu.co/handle/10336/26011engElsevier781No. 3771Biochemical and Biophysical Research CommunicationsVol. 332Biochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.332, No.3 (2005); pp.771-781https://www.sciencedirect.com/science/article/abs/pii/S0006291X05009642Restringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecBiochemical and Biophysical Research Communicationsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURRv1510Mycobacterium tuberculosisTuberculosisPeptidesBindingCharacterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell linesCaracterizando la proteína Mycobacterium tuberculosis Rv1510c y determinando sus secuencias que se unen específicamente a dos líneas celulares dianaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Vera-Bravo, RicardoTorres, ElizabethValbuena, John J.Rodríguez, Luis E.Puentes, ÁlvaroGarcía, Javier E.Curtidor, HernandoCortés, JimenaVanegas, MagnoliaRivera, Zuly J.Díaz, AndreaCalderon, Martha N.Patarroyo, Manuel A.Patarroyo, Manuel E.Ocampo, Marisol10336/26011oai:repository.urosario.edu.co:10336/260112022-05-02 07:37:21.807888https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
dc.title.TranslatedTitle.spa.fl_str_mv Caracterizando la proteína Mycobacterium tuberculosis Rv1510c y determinando sus secuencias que se unen específicamente a dos líneas celulares diana
title Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
spellingShingle Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
Rv1510
Mycobacterium tuberculosis
Tuberculosis
Peptides
Binding
title_short Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
title_full Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
title_fullStr Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
title_full_unstemmed Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
title_sort Characterising Mycobacterium tuberculosis Rv1510c protein and determining its sequences that specifically bind to two target cell lines
dc.subject.keyword.spa.fl_str_mv Rv1510
Mycobacterium tuberculosis
Tuberculosis
Peptides
Binding
topic Rv1510
Mycobacterium tuberculosis
Tuberculosis
Peptides
Binding
description The process of Mycobacterium tuberculosis infection of the macrophage implies a very little-known initial recognition and adherence step, important for mycobacterial survival; many proteins even remain like hypothetical. The Rv1510c gene, encoding a putatively conserved membrane protein, was investigated by analysing the M. tuberculosis genome sequence data reported by Cole et al. and a previous report that used PCR assays to show that the Rv1510 gene was only present in M. tuberculosis. This article confirmed all the above and identified the transcribed gene in M. tuberculosis, Mycobacterium africanum, and in M. tuberculosis clinical isolates. Antibodies raised against peptides from this protein recognised a 44 kDa band, corresponding to Rv1510c theoretical mass (44,294 Da). Assays involving synthetic peptides covering the whole protein binding to U937 and A549 cell lines led to recognising five high activity binding peptides in the Rv1510 protein: 11094, 11095, 11105, 11108, and 11111. Their affinity constants and Hill coefficients were determined by using U937 cells. Cross-linking assays performed with some of these HABPs showed that they specifically bound to a U937 cell line 51 kDa protein, but not to Hep G2 or red blood cell proteins, showing this interaction’s specificity.
publishDate 2005
dc.date.created.spa.fl_str_mv 2005-07-08
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:20:27Z
dc.date.available.none.fl_str_mv 2020-08-06T16:20:27Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.bbrc.2005.05.018
dc.identifier.issn.none.fl_str_mv ISSN: 0006-291X
EISSN: 1090-2104
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26011
url https://doi.org/10.1016/j.bbrc.2005.05.018
https://repository.urosario.edu.co/handle/10336/26011
identifier_str_mv ISSN: 0006-291X
EISSN: 1090-2104
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 781
dc.relation.citationIssue.none.fl_str_mv No. 3
dc.relation.citationStartPage.none.fl_str_mv 771
dc.relation.citationTitle.none.fl_str_mv Biochemical and Biophysical Research Communications
dc.relation.citationVolume.none.fl_str_mv Vol. 332
dc.relation.ispartof.spa.fl_str_mv Biochemical and Biophysical Research Communications, ISSN: 0006-291X;EISSN: 1090-2104, Vol.332, No.3 (2005); pp.771-781
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S0006291X05009642
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier
dc.source.spa.fl_str_mv Biochemical and Biophysical Research Communications
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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