Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection

Parasite antigen genetic diversity represents a great obstacle when designing a vaccine against malaria caused by Plasmodium vivax. Selecting vaccine candidate antigens has been focused on those fulfilling a role in invasion and which are conserved, thus avoiding specific-allele immune responses. Mo...

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Autores:
Tipo de recurso:
Fecha de publicación:
2018
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/21307
Acceso en línea:
https://doi.org/10.3389/fgene.2018.00010
https://repository.urosario.edu.co/handle/10336/21307
Palabra clave:
Plasmodium vivax
Genetic diversity
Sporozoite
Vaccine
Natural selection
Hepatocyte invasion
Enfermedades
Plasmodium vivax
Plasmodium vivax
Diversidad genetica
Esporozoıto
Seleccion natural
Rights
License
Abierto (Texto Completo)
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repository_id_str
dc.title.spa.fl_str_mv Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
title Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
spellingShingle Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
Plasmodium vivax
Genetic diversity
Sporozoite
Vaccine
Natural selection
Hepatocyte invasion
Enfermedades
Plasmodium vivax
Plasmodium vivax
Diversidad genetica
Esporozoıto
Seleccion natural
title_short Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
title_full Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
title_fullStr Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
title_full_unstemmed Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
title_sort Identifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selection
dc.subject.spa.fl_str_mv Plasmodium vivax
Genetic diversity
Sporozoite
Vaccine
Natural selection
Hepatocyte invasion
topic Plasmodium vivax
Genetic diversity
Sporozoite
Vaccine
Natural selection
Hepatocyte invasion
Enfermedades
Plasmodium vivax
Plasmodium vivax
Diversidad genetica
Esporozoıto
Seleccion natural
dc.subject.ddc.spa.fl_str_mv Enfermedades
dc.subject.decs.spa.fl_str_mv Plasmodium vivax
dc.subject.lemb.spa.fl_str_mv Plasmodium vivax
Diversidad genetica
Esporozoıto
Seleccion natural
description Parasite antigen genetic diversity represents a great obstacle when designing a vaccine against malaria caused by Plasmodium vivax. Selecting vaccine candidate antigens has been focused on those fulfilling a role in invasion and which are conserved, thus avoiding specific-allele immune responses. Most antigens described to date belong to the blood stage, thereby blocking parasite development within red blood cells, whilst studying antigens from other stages has been quite restricted. Antigens from different parasite stages are required for developing a completely effective vaccine; thus, pre-erythrocyte stage antigens able to block the first line of infection becoming established should also be taken into account. However, few antigens from this stage have been studied to date. Several P. falciparum sporozoite antigens are involved in invasion. Since 77% of genes are orthologous amongst Plasmodium parasites, P. vivax sporozoite antigen orthologs to those of P. falciparum might be present in its genome. Although these genes might have high genetic diversity, conserved functionally-relevant regions (ideal for vaccine development) could be predicted by comparing genetic diversity patterns and evolutionary rates. This study was thus aimed at searching for putative P. vivax sporozoite genes so as to analyse their genetic diversity for determining their potential as vaccine candidates. Several DNA sequence polymorphism estimators were computed at each locus. The evolutionary force (drift, selection and recombination) drawing the genetic diversity pattern observed was also determined by using tests based on polymorphism frequency spectrum as well as the type of intra- and inter-species substitutions. Likewise, recombination was assessed both indirectly and directly. The results showed that sporozoite genes were more conserved than merozoite genes evaluated to date. Putative domains implied in cell traversal, gliding motility and hepatocyte interaction had a negative selection signal, being conserved amongst different species in the genus. PvP52, PvP36, PvSPATR, PvPLP1, PvMCP1, PvTLP, PvCelTOS, and PvMB2 antigens or functionally restricted regions within them would thus seem promising vaccine candidates and could be used when designing a pre-erythrocyte and/or multi-stage vaccine against P. vivax to avoid allele-specific immune responses that could reduce vaccine efficacy. © 2018 Garzón-Ospina, Buitrago, Ramos and Patarroyo.
publishDate 2018
dc.date.created.none.fl_str_mv 2018-01-25
dc.date.issued.none.fl_str_mv 2018
dc.date.accessioned.none.fl_str_mv 2020-03-31T00:26:13Z
dc.date.available.none.fl_str_mv 2020-03-31T00:26:13Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.3389/fgene.2018.00010
dc.identifier.issn.none.fl_str_mv 1664-8021
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/21307
url https://doi.org/10.3389/fgene.2018.00010
https://repository.urosario.edu.co/handle/10336/21307
identifier_str_mv 1664-8021
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationTitle.none.fl_str_mv Frontiers in Genetics
dc.relation.citationVolume.none.fl_str_mv Vol. 9
dc.relation.ispartof.spa.fl_str_mv Frontiers in Genetics, ISSN:1664-8021, Vol. 9 (2018)
dc.relation.uri.spa.fl_str_mv https://www.frontiersin.org/articles/10.3389/fgene.2018.00010/full
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institution Universidad del Rosario
dc.source.bibliographicCitation.spa.fl_str_mv Abascal, F., Zardoya, R., Telford, M.J., TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations (2010) Nucleic Acids Res, 38, pp. W7-W13
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling 6d7ac92b-6f18-415f-8419-0943baaa883c600d260129f-dd6b-47a9-9224-43d1cad0695e60064ed3111-4a4c-4583-a875-5201cdec7cfc600796530656002020-03-31T00:26:13Z2020-03-31T00:26:13Z2018-01-252018Parasite antigen genetic diversity represents a great obstacle when designing a vaccine against malaria caused by Plasmodium vivax. Selecting vaccine candidate antigens has been focused on those fulfilling a role in invasion and which are conserved, thus avoiding specific-allele immune responses. Most antigens described to date belong to the blood stage, thereby blocking parasite development within red blood cells, whilst studying antigens from other stages has been quite restricted. Antigens from different parasite stages are required for developing a completely effective vaccine; thus, pre-erythrocyte stage antigens able to block the first line of infection becoming established should also be taken into account. However, few antigens from this stage have been studied to date. Several P. falciparum sporozoite antigens are involved in invasion. Since 77% of genes are orthologous amongst Plasmodium parasites, P. vivax sporozoite antigen orthologs to those of P. falciparum might be present in its genome. Although these genes might have high genetic diversity, conserved functionally-relevant regions (ideal for vaccine development) could be predicted by comparing genetic diversity patterns and evolutionary rates. This study was thus aimed at searching for putative P. vivax sporozoite genes so as to analyse their genetic diversity for determining their potential as vaccine candidates. Several DNA sequence polymorphism estimators were computed at each locus. The evolutionary force (drift, selection and recombination) drawing the genetic diversity pattern observed was also determined by using tests based on polymorphism frequency spectrum as well as the type of intra- and inter-species substitutions. Likewise, recombination was assessed both indirectly and directly. The results showed that sporozoite genes were more conserved than merozoite genes evaluated to date. Putative domains implied in cell traversal, gliding motility and hepatocyte interaction had a negative selection signal, being conserved amongst different species in the genus. PvP52, PvP36, PvSPATR, PvPLP1, PvMCP1, PvTLP, PvCelTOS, and PvMB2 antigens or functionally restricted regions within them would thus seem promising vaccine candidates and could be used when designing a pre-erythrocyte and/or multi-stage vaccine against P. vivax to avoid allele-specific immune responses that could reduce vaccine efficacy. © 2018 Garzón-Ospina, Buitrago, Ramos and Patarroyo.application/pdfhttps://doi.org/10.3389/fgene.2018.000101664-8021https://repository.urosario.edu.co/handle/10336/21307engFrontiers in GeneticsVol. 9Frontiers in Genetics, ISSN:1664-8021, Vol. 9 (2018)https://www.frontiersin.org/articles/10.3389/fgene.2018.00010/fullAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Abascal, F., Zardoya, R., Telford, M.J., TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations (2010) Nucleic Acids Res, 38, pp. W7-W13instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURPlasmodium vivaxGenetic diversitySporozoiteVaccineNatural selectionHepatocyte invasionEnfermedades616600Plasmodium vivaxPlasmodium vivaxDiversidad geneticaEsporozoıtoSeleccion naturalIdentifying potential Plasmodium vivax sporozoite stage vaccine candidates : An analysis of genetic diversity and natural selectionarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Garzón-Ospina, DiegoBuitrago, SindyRamos, Andrea E.Patarroyo, Manuel A.Garzón-Ospina, DiegoBuitrago, Sindy P.Ramos, Andrea E.Patarroyo, Manuel A.ORIGINAL92.pdfapplication/pdf783299https://repository.urosario.edu.co/bitstreams/49a35253-3f09-4336-a09a-ddb061b64e57/download9ea8d32fecd6f3f33eb0993f3a767603MD51TEXT92.pdf.txt92.pdf.txtExtracted texttext/plain90682https://repository.urosario.edu.co/bitstreams/9dd8b91a-93b8-4617-a6a5-b2825e53d869/downloadb5e0c96418d1c0e8d07cf803713e17d9MD52THUMBNAIL92.pdf.jpg92.pdf.jpgGenerated Thumbnailimage/jpeg4469https://repository.urosario.edu.co/bitstreams/f77d561f-9fde-4c8a-a9de-32fb399686b2/download7cacd97162b672db6c1a2962e39bee13MD5310336/21307oai:repository.urosario.edu.co:10336/213072020-05-13 14:47:41.839https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co