Determining the Plasmodium vivax VCG-1 strain blood stage proteome

Plasmodium vivax is the second most prevalent parasite species causing malaria in humans living in tropical and subtropical areas throughout the world. There have been few P. vivax proteomic studies to date and they have focused on using clinical isolates, given the technical difficulties concerning...

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Autores:
Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/22438
Acceso en línea:
https://doi.org/10.1016/j.jprot.2014.10.003
https://repository.urosario.edu.co/handle/10336/22438
Palabra clave:
Proteome
Transcriptome
Animal experiment
Article
Blood analysis
Controlled study
Data base
In vitro study
Life cycle assessment
Liquid chromatography
Malaria
Mass spectrometry
Molecular genetics
Nonhuman
Parasite identification
Parasite virulence
Plasmodium vivax
Primate model
Protein analysis
Proteomics
Public health problem
Reticulocyte
Plasmodium vivax
Biology
Mass spectrometry
P
Vivax
Proteins
Proteome
Rights
License
Abierto (Texto Completo)
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dc.title.spa.fl_str_mv Determining the Plasmodium vivax VCG-1 strain blood stage proteome
title Determining the Plasmodium vivax VCG-1 strain blood stage proteome
spellingShingle Determining the Plasmodium vivax VCG-1 strain blood stage proteome
Proteome
Transcriptome
Animal experiment
Article
Blood analysis
Controlled study
Data base
In vitro study
Life cycle assessment
Liquid chromatography
Malaria
Mass spectrometry
Molecular genetics
Nonhuman
Parasite identification
Parasite virulence
Plasmodium vivax
Primate model
Protein analysis
Proteomics
Public health problem
Reticulocyte
Plasmodium vivax
Biology
Mass spectrometry
P
Vivax
Proteins
Proteome
title_short Determining the Plasmodium vivax VCG-1 strain blood stage proteome
title_full Determining the Plasmodium vivax VCG-1 strain blood stage proteome
title_fullStr Determining the Plasmodium vivax VCG-1 strain blood stage proteome
title_full_unstemmed Determining the Plasmodium vivax VCG-1 strain blood stage proteome
title_sort Determining the Plasmodium vivax VCG-1 strain blood stage proteome
dc.subject.keyword.spa.fl_str_mv Proteome
Transcriptome
Animal experiment
Article
Blood analysis
Controlled study
Data base
In vitro study
Life cycle assessment
Liquid chromatography
Malaria
Mass spectrometry
Molecular genetics
Nonhuman
Parasite identification
Parasite virulence
Plasmodium vivax
Primate model
Protein analysis
Proteomics
Public health problem
Reticulocyte
Plasmodium vivax
Biology
Mass spectrometry
P
Vivax
Proteins
Proteome
topic Proteome
Transcriptome
Animal experiment
Article
Blood analysis
Controlled study
Data base
In vitro study
Life cycle assessment
Liquid chromatography
Malaria
Mass spectrometry
Molecular genetics
Nonhuman
Parasite identification
Parasite virulence
Plasmodium vivax
Primate model
Protein analysis
Proteomics
Public health problem
Reticulocyte
Plasmodium vivax
Biology
Mass spectrometry
P
Vivax
Proteins
Proteome
description Plasmodium vivax is the second most prevalent parasite species causing malaria in humans living in tropical and subtropical areas throughout the world. There have been few P. vivax proteomic studies to date and they have focused on using clinical isolates, given the technical difficulties concerning how to maintain an in vitro culture of this species. This study was thus focused on identifying the P. vivax VCG-1 strain proteome during its blood lifecycle through LC-MS/MS; this led to identifying 734 proteins, thus increasing the overall number reported for P. vivax to date. Some of them have previously been related to reticulocyte invasion, parasite virulence and growth and others are new molecules possibly playing a functional role during metabolic processes, as predicted by Database for Annotation, Visualization and Integrated Discovery (DAVID) functional analysis. This is the first large-scale proteomic analysis of a P. vivax strain adapted to a non-human primate model showing the parasite protein repertoire during the blood lifecycle. Database searches facilitated the in silico prediction of proteins proposed for evaluation in further experimental assays regarding their potential as pharmacologic targets or as component of a totally efficient vaccine against malaria caused by P. vivax. Biological significance: P. vivax malaria continues being a public health problem around world. Although considerable progress has been made in understanding genome- and transcriptome-related P. vivax biology, there are few proteome studies, currently representing only 8.5% of the predicted in silico proteome reported in public databases. A high-throughput proteomic assay was used for discovering new P. vivax intra-reticulocyte asexual stage molecules taken from parasites maintained in vivo in a primate model. The methodology avoided the main problem related to standardising an in vitro culture system to obtain enough samples for protein identification and annotation. This study provides a source of potential information contributing towards a basic understanding of P. vivax biology related to parasite proteins which are of significant importance for the malaria research community. © 2014 .
publishDate 2015
dc.date.created.spa.fl_str_mv 2015
dc.date.accessioned.none.fl_str_mv 2020-05-25T23:56:29Z
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dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.jprot.2014.10.003
dc.identifier.issn.none.fl_str_mv 18743919
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dc.relation.citationTitle.none.fl_str_mv Journal of Proteomics
dc.relation.citationVolume.none.fl_str_mv Vol. 113
dc.relation.ispartof.spa.fl_str_mv Journal of Proteomics, ISSN:18743919, Vol.113,(2015); pp. 268-280
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spelling 7dc8541b-968d-46c5-b738-346e35bad888-1e1a31982-761b-4535-ae78-1d11cad494bc-17c543899-e350-48b1-ad71-cff6366ffa5d-14b9bcdd1-0bf2-49e4-8a4b-27fc1fce08af-19fc64f6d-a903-48f1-ac2e-4e55fd2ed9af-12020-05-25T23:56:29Z2020-05-25T23:56:29Z2015Plasmodium vivax is the second most prevalent parasite species causing malaria in humans living in tropical and subtropical areas throughout the world. There have been few P. vivax proteomic studies to date and they have focused on using clinical isolates, given the technical difficulties concerning how to maintain an in vitro culture of this species. This study was thus focused on identifying the P. vivax VCG-1 strain proteome during its blood lifecycle through LC-MS/MS; this led to identifying 734 proteins, thus increasing the overall number reported for P. vivax to date. Some of them have previously been related to reticulocyte invasion, parasite virulence and growth and others are new molecules possibly playing a functional role during metabolic processes, as predicted by Database for Annotation, Visualization and Integrated Discovery (DAVID) functional analysis. This is the first large-scale proteomic analysis of a P. vivax strain adapted to a non-human primate model showing the parasite protein repertoire during the blood lifecycle. Database searches facilitated the in silico prediction of proteins proposed for evaluation in further experimental assays regarding their potential as pharmacologic targets or as component of a totally efficient vaccine against malaria caused by P. vivax. Biological significance: P. vivax malaria continues being a public health problem around world. Although considerable progress has been made in understanding genome- and transcriptome-related P. vivax biology, there are few proteome studies, currently representing only 8.5% of the predicted in silico proteome reported in public databases. A high-throughput proteomic assay was used for discovering new P. vivax intra-reticulocyte asexual stage molecules taken from parasites maintained in vivo in a primate model. The methodology avoided the main problem related to standardising an in vitro culture system to obtain enough samples for protein identification and annotation. This study provides a source of potential information contributing towards a basic understanding of P. vivax biology related to parasite proteins which are of significant importance for the malaria research community. © 2014 .application/pdfhttps://doi.org/10.1016/j.jprot.2014.10.00318743919https://repository.urosario.edu.co/handle/10336/22438engElsevier280268Journal of ProteomicsVol. 113Journal of Proteomics, ISSN:18743919, Vol.113,(2015); pp. 268-280https://www.scopus.com/inward/record.uri?eid=2-s2.0-84908316303&doi=10.1016%2fj.jprot.2014.10.003&partnerID=40&md5=eae5981b1f09b98845e3f08fe7194db7Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURProteomeTranscriptomeAnimal experimentArticleBlood analysisControlled studyData baseIn vitro studyLife cycle assessmentLiquid chromatographyMalariaMass spectrometryMolecular geneticsNonhumanParasite identificationParasite virulencePlasmodium vivaxPrimate modelProtein analysisProteomicsPublic health problemReticulocytePlasmodium vivaxBiologyMass spectrometryPVivaxProteinsProteomeDetermining the Plasmodium vivax VCG-1 strain blood stage proteomearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Moreno-Pérez, D.A.Dégano, R.Ibarrola, N.Muro, A.Patarroyo, M.A.ORIGINAL1-s2-0-S1874391914004679-main.pdfapplication/pdf880383https://repository.urosario.edu.co/bitstreams/388f742c-1b54-47c9-8037-d46abde3444a/downloada25e3db5670d0159c6a193bfb51b9162MD51TEXT1-s2-0-S1874391914004679-main.pdf.txt1-s2-0-S1874391914004679-main.pdf.txtExtracted texttext/plain67723https://repository.urosario.edu.co/bitstreams/fbfbbcdb-77ec-4237-8af0-f8b8a27fc5e5/download555977cd4eb25bc5b6b8eeef3e527f19MD52THUMBNAIL1-s2-0-S1874391914004679-main.pdf.jpg1-s2-0-S1874391914004679-main.pdf.jpgGenerated Thumbnailimage/jpeg4386https://repository.urosario.edu.co/bitstreams/88acb77f-4fc9-4172-8a61-7ca1da74ea52/download8e84b0add2d16b010d0bc991735b7beeMD5310336/22438oai:repository.urosario.edu.co:10336/224382022-05-02 07:37:14.17734https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co