Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8

Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth...

Full description

Autores:
Tipo de recurso:
Fecha de publicación:
2000
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27142
Acceso en línea:
https://doi.org/10.1128/JB.182.8.2179-2183.2000
https://repository.urosario.edu.co/handle/10336/27142
Palabra clave:
Anion transport proteins
Bacterial proteins genetics
Carrier proteins genetics
Nitrates metabolism
Nitrites metabolism
Thermus thermophilus genetics
Rights
License
Abierto (Texto Completo)
id EDOCUR2_e25c67699069ea8d5f8fc31f4da605c6
oai_identifier_str oai:repository.urosario.edu.co:10336/27142
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 518662516006cb90c8e-1835-4367-8822-9e074e82adf8-186fe53aa-7765-4602-bf07-2bafd709d957-19d97dca4-4346-41f0-8795-7ee0d7efc4dc-13e637842-a6dc-445e-9785-b76936ec3ca1-16fb7b8d4-9b72-485d-8cc9-c5f30caf0cf5-12020-08-19T14:41:07Z2020-08-19T14:41:07Z2000-04-15Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its active extrusion from the cell. We describe the presence of two genes,narK1 and narK2, downstream of the nitrate reductase-encoding gene cluster (nar) that code for two homologues to the major facilitator superfamily of transporters. The sequences of NarK1 and NarK2 are 30% identical to each other, but whereas NarK1 clusters in an average-distance tree with putative nitrate transporters, NarK2 does so with putative nitrite exporters. To analyze whether this differential clustering was actually related to functional differences, we isolated derivatives with mutations of one or both genes. Analysis revealed that single mutations had minor effects on growth by nitrate respiration, whereas a double narK1 narK2 mutation abolished this capability. Further analysis allowed us to confirm that the double mutant is completely unable to excrete nitrite, while single mutants have a limitation in the excretion rates compared with the wild type. These data allow us to propose that both proteins are implicated in the transport of nitrate and nitrite, probably acting as nitrate/nitrite antiporters. The possible differential roles of these proteins in vivo are discussed.application/pdfhttps://doi.org/10.1128/JB.182.8.2179-2183.2000ISSN: 0021-9193EISSN: 1098-5530https://repository.urosario.edu.co/handle/10336/27142engAmerican Society for Microbiology2183No. 82179Journal of BacteriologyVol. 182Journal of Bacteriology, ISSN: 0021-9193;EISSN: 1098-5530, Vol.182, No.8 (April 2000); pp. 2179-2183https://jb.asm.org/content/jb/182/8/2179.full.pdfAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Journal of Bacteriologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAnion transport proteinsBacterial proteins geneticsCarrier proteins geneticsNitrates metabolismNitrites metabolismThermus thermophilus geneticsTwo nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8Dos transportadores de nitrato / nitrito están codificados dentro del plásmido movilizable para la respiración de nitrato de Thermus thermophilus HB8articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Ramírez Clavijo, Sandra RocíoMoreno, RenataZafra, OlgaCastán, PabloVallés, CristinaBerenguer, JoséORIGINALJournal-of-Bacteriology-2000-Ramirez-2179-full.pdfapplication/pdf540187https://repository.urosario.edu.co/bitstreams/309d07ea-ef2c-40a9-ad6b-097398e1cadc/downloade6b13e282fffbaf07255ef26cd83403dMD51TEXTJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.txtJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.txtExtracted texttext/plain28756https://repository.urosario.edu.co/bitstreams/39d4c290-dbe5-4504-aa65-b859dd662853/downloada44e1be9ee4c2a82399cbfd478041ef3MD52THUMBNAILJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.jpgJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.jpgGenerated Thumbnailimage/jpeg5039https://repository.urosario.edu.co/bitstreams/0b350e67-f4a8-4e30-bd3b-aa95cc3600eb/downloadb9284b293fd36036dc750cd72ef091a2MD5310336/27142oai:repository.urosario.edu.co:10336/271422021-06-03 00:50:06.462https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
dc.title.TranslatedTitle.spa.fl_str_mv Dos transportadores de nitrato / nitrito están codificados dentro del plásmido movilizable para la respiración de nitrato de Thermus thermophilus HB8
title Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
spellingShingle Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
Anion transport proteins
Bacterial proteins genetics
Carrier proteins genetics
Nitrates metabolism
Nitrites metabolism
Thermus thermophilus genetics
title_short Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
title_full Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
title_fullStr Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
title_full_unstemmed Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
title_sort Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
dc.subject.keyword.spa.fl_str_mv Anion transport proteins
Bacterial proteins genetics
Carrier proteins genetics
Nitrates metabolism
Nitrites metabolism
Thermus thermophilus genetics
topic Anion transport proteins
Bacterial proteins genetics
Carrier proteins genetics
Nitrates metabolism
Nitrites metabolism
Thermus thermophilus genetics
description Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its active extrusion from the cell. We describe the presence of two genes,narK1 and narK2, downstream of the nitrate reductase-encoding gene cluster (nar) that code for two homologues to the major facilitator superfamily of transporters. The sequences of NarK1 and NarK2 are 30% identical to each other, but whereas NarK1 clusters in an average-distance tree with putative nitrate transporters, NarK2 does so with putative nitrite exporters. To analyze whether this differential clustering was actually related to functional differences, we isolated derivatives with mutations of one or both genes. Analysis revealed that single mutations had minor effects on growth by nitrate respiration, whereas a double narK1 narK2 mutation abolished this capability. Further analysis allowed us to confirm that the double mutant is completely unable to excrete nitrite, while single mutants have a limitation in the excretion rates compared with the wild type. These data allow us to propose that both proteins are implicated in the transport of nitrate and nitrite, probably acting as nitrate/nitrite antiporters. The possible differential roles of these proteins in vivo are discussed.
publishDate 2000
dc.date.created.spa.fl_str_mv 2000-04-15
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:41:07Z
dc.date.available.none.fl_str_mv 2020-08-19T14:41:07Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1128/JB.182.8.2179-2183.2000
dc.identifier.issn.none.fl_str_mv ISSN: 0021-9193
EISSN: 1098-5530
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27142
url https://doi.org/10.1128/JB.182.8.2179-2183.2000
https://repository.urosario.edu.co/handle/10336/27142
identifier_str_mv ISSN: 0021-9193
EISSN: 1098-5530
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 2183
dc.relation.citationIssue.none.fl_str_mv No. 8
dc.relation.citationStartPage.none.fl_str_mv 2179
dc.relation.citationTitle.none.fl_str_mv Journal of Bacteriology
dc.relation.citationVolume.none.fl_str_mv Vol. 182
dc.relation.ispartof.spa.fl_str_mv Journal of Bacteriology, ISSN: 0021-9193;EISSN: 1098-5530, Vol.182, No.8 (April 2000); pp. 2179-2183
dc.relation.uri.spa.fl_str_mv https://jb.asm.org/content/jb/182/8/2179.full.pdf
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv American Society for Microbiology
dc.source.spa.fl_str_mv Journal of Bacteriology
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
bitstream.url.fl_str_mv https://repository.urosario.edu.co/bitstreams/309d07ea-ef2c-40a9-ad6b-097398e1cadc/download
https://repository.urosario.edu.co/bitstreams/39d4c290-dbe5-4504-aa65-b859dd662853/download
https://repository.urosario.edu.co/bitstreams/0b350e67-f4a8-4e30-bd3b-aa95cc3600eb/download
bitstream.checksum.fl_str_mv e6b13e282fffbaf07255ef26cd83403d
a44e1be9ee4c2a82399cbfd478041ef3
b9284b293fd36036dc750cd72ef091a2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167653935218688