Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2000
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/27142
- Acceso en línea:
- https://doi.org/10.1128/JB.182.8.2179-2183.2000
https://repository.urosario.edu.co/handle/10336/27142
- Palabra clave:
- Anion transport proteins
Bacterial proteins genetics
Carrier proteins genetics
Nitrates metabolism
Nitrites metabolism
Thermus thermophilus genetics
- Rights
- License
- Abierto (Texto Completo)
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518662516006cb90c8e-1835-4367-8822-9e074e82adf8-186fe53aa-7765-4602-bf07-2bafd709d957-19d97dca4-4346-41f0-8795-7ee0d7efc4dc-13e637842-a6dc-445e-9785-b76936ec3ca1-16fb7b8d4-9b72-485d-8cc9-c5f30caf0cf5-12020-08-19T14:41:07Z2020-08-19T14:41:07Z2000-04-15Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its active extrusion from the cell. We describe the presence of two genes,narK1 and narK2, downstream of the nitrate reductase-encoding gene cluster (nar) that code for two homologues to the major facilitator superfamily of transporters. The sequences of NarK1 and NarK2 are 30% identical to each other, but whereas NarK1 clusters in an average-distance tree with putative nitrate transporters, NarK2 does so with putative nitrite exporters. To analyze whether this differential clustering was actually related to functional differences, we isolated derivatives with mutations of one or both genes. Analysis revealed that single mutations had minor effects on growth by nitrate respiration, whereas a double narK1 narK2 mutation abolished this capability. Further analysis allowed us to confirm that the double mutant is completely unable to excrete nitrite, while single mutants have a limitation in the excretion rates compared with the wild type. These data allow us to propose that both proteins are implicated in the transport of nitrate and nitrite, probably acting as nitrate/nitrite antiporters. The possible differential roles of these proteins in vivo are discussed.application/pdfhttps://doi.org/10.1128/JB.182.8.2179-2183.2000ISSN: 0021-9193EISSN: 1098-5530https://repository.urosario.edu.co/handle/10336/27142engAmerican Society for Microbiology2183No. 82179Journal of BacteriologyVol. 182Journal of Bacteriology, ISSN: 0021-9193;EISSN: 1098-5530, Vol.182, No.8 (April 2000); pp. 2179-2183https://jb.asm.org/content/jb/182/8/2179.full.pdfAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Journal of Bacteriologyinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAnion transport proteinsBacterial proteins geneticsCarrier proteins geneticsNitrates metabolismNitrites metabolismThermus thermophilus geneticsTwo nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8Dos transportadores de nitrato / nitrito están codificados dentro del plásmido movilizable para la respiración de nitrato de Thermus thermophilus HB8articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Ramírez Clavijo, Sandra RocíoMoreno, RenataZafra, OlgaCastán, PabloVallés, CristinaBerenguer, JoséORIGINALJournal-of-Bacteriology-2000-Ramirez-2179-full.pdfapplication/pdf540187https://repository.urosario.edu.co/bitstreams/309d07ea-ef2c-40a9-ad6b-097398e1cadc/downloade6b13e282fffbaf07255ef26cd83403dMD51TEXTJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.txtJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.txtExtracted texttext/plain28756https://repository.urosario.edu.co/bitstreams/39d4c290-dbe5-4504-aa65-b859dd662853/downloada44e1be9ee4c2a82399cbfd478041ef3MD52THUMBNAILJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.jpgJournal-of-Bacteriology-2000-Ramirez-2179-full.pdf.jpgGenerated Thumbnailimage/jpeg5039https://repository.urosario.edu.co/bitstreams/0b350e67-f4a8-4e30-bd3b-aa95cc3600eb/downloadb9284b293fd36036dc750cd72ef091a2MD5310336/27142oai:repository.urosario.edu.co:10336/271422021-06-03 00:50:06.462https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
dc.title.TranslatedTitle.spa.fl_str_mv |
Dos transportadores de nitrato / nitrito están codificados dentro del plásmido movilizable para la respiración de nitrato de Thermus thermophilus HB8 |
title |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
spellingShingle |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 Anion transport proteins Bacterial proteins genetics Carrier proteins genetics Nitrates metabolism Nitrites metabolism Thermus thermophilus genetics |
title_short |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
title_full |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
title_fullStr |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
title_full_unstemmed |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
title_sort |
Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8 |
dc.subject.keyword.spa.fl_str_mv |
Anion transport proteins Bacterial proteins genetics Carrier proteins genetics Nitrates metabolism Nitrites metabolism Thermus thermophilus genetics |
topic |
Anion transport proteins Bacterial proteins genetics Carrier proteins genetics Nitrates metabolism Nitrites metabolism Thermus thermophilus genetics |
description |
Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its active extrusion from the cell. We describe the presence of two genes,narK1 and narK2, downstream of the nitrate reductase-encoding gene cluster (nar) that code for two homologues to the major facilitator superfamily of transporters. The sequences of NarK1 and NarK2 are 30% identical to each other, but whereas NarK1 clusters in an average-distance tree with putative nitrate transporters, NarK2 does so with putative nitrite exporters. To analyze whether this differential clustering was actually related to functional differences, we isolated derivatives with mutations of one or both genes. Analysis revealed that single mutations had minor effects on growth by nitrate respiration, whereas a double narK1 narK2 mutation abolished this capability. Further analysis allowed us to confirm that the double mutant is completely unable to excrete nitrite, while single mutants have a limitation in the excretion rates compared with the wild type. These data allow us to propose that both proteins are implicated in the transport of nitrate and nitrite, probably acting as nitrate/nitrite antiporters. The possible differential roles of these proteins in vivo are discussed. |
publishDate |
2000 |
dc.date.created.spa.fl_str_mv |
2000-04-15 |
dc.date.accessioned.none.fl_str_mv |
2020-08-19T14:41:07Z |
dc.date.available.none.fl_str_mv |
2020-08-19T14:41:07Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1128/JB.182.8.2179-2183.2000 |
dc.identifier.issn.none.fl_str_mv |
ISSN: 0021-9193 EISSN: 1098-5530 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/27142 |
url |
https://doi.org/10.1128/JB.182.8.2179-2183.2000 https://repository.urosario.edu.co/handle/10336/27142 |
identifier_str_mv |
ISSN: 0021-9193 EISSN: 1098-5530 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
2183 |
dc.relation.citationIssue.none.fl_str_mv |
No. 8 |
dc.relation.citationStartPage.none.fl_str_mv |
2179 |
dc.relation.citationTitle.none.fl_str_mv |
Journal of Bacteriology |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 182 |
dc.relation.ispartof.spa.fl_str_mv |
Journal of Bacteriology, ISSN: 0021-9193;EISSN: 1098-5530, Vol.182, No.8 (April 2000); pp. 2179-2183 |
dc.relation.uri.spa.fl_str_mv |
https://jb.asm.org/content/jb/182/8/2179.full.pdf |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
American Society for Microbiology |
dc.source.spa.fl_str_mv |
Journal of Bacteriology |
institution |
Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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