Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability

Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at perfor...

Full description

Autores:

Tipo de recurso:

Fecha de publicación:: 2018

Institución:: Universidad del Rosario

Repositorio:: Repositorio EdocUR - U. Rosario

Idioma:: eng

id	EDOCUR2_e1d4fa1ceed4b285e564ebfff27c2425
oai_identifier_str	oai:repository.urosario.edu.co:10336/23462
network_acronym_str	EDOCUR2
network_name_str	Repositorio EdocUR - U. Rosario
repository_id_str
spelling	315c69f4-b258-4792-8e15-b670985216ad-11388956c-8ca1-40b8-b5de-4a01ca2df82b-1978a6d48-707d-4d65-8840-0dedad07d04e-1fc887a88-c667-4d3f-8600-d2183f4dd565d0109870-a6c3-4dae-ac6f-6d956b81cba8-19fc64f6d-a903-48f1-ac2e-4e55fd2ed9af-19bb3b964-c097-425e-a69c-243d0d0573d7-14bcd1d4a-bd03-4e3a-aad0-d40446559d53-12020-05-26T00:02:14Z2020-05-26T00:02:14Z2018Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended. © 2018 Elsevier Ltdapplication/pdfhttps://doi.org/10.1016/j.vaccine.2018.09.0440264410X13588745https://repository.urosario.edu.co/handle/10336/23462engElsevier Ltd6710No. 456703VaccineVol. 36Vaccine, ISSN:0264410X, 13588745, Vol.36, No.45 (2018); pp. 6703-6710https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053915548&doi=10.1016%2fj.vaccine.2018.09.044&partnerID=40&md5=99a8717593b22d1e9b7987722393073dAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURCircumsporozoite like antigen vaccineFreund adjuvantNeutralizing antibodyPeptide vaccineProtozoal vaccineSodium chlorideSporozoite surface antigen 15 vaccineUnclassified drugParasite antigenPeptideProtozoal dnaProtozoal proteinProtozoal vaccineProtozoon antibodyAnimal experimentAntibody detectionAntibody productionAntibody responseArticleBioinformaticsCell cultureControlled studyCryptosporidiosisCryptosporidium parvumDrug formulationDrug synthesisEnzyme linked immunosorbent assayFemaleHumoral immunityImmunological proceduresIn vitro studyIn vivo studyMouseNonhumanNucleotide sequencePeptide analysisPriority journalSeroneutralization testSwiss webster mouseVaccine immunogenicityChemistryImmunologySynthesisCryptosporidium parvumPeptidesProtozoan proteinsProtozoan vaccinesCp15CryptosporidiosisCryptosporidiumCslPeptideVaccineprotozoanprotozoanprotozoanAntibodiesAntigensDnaCryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capabilityarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Avendaño C.Jenkins M.Méndez-Callejas G.Oviedo, JairoGuzmán F.Patarroyo M.A.Sánchez-Acedo C.Quílez J.10336/23462oai:repository.urosario.edu.co:10336/234622023-06-02 15:12:45.909https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
title	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
spellingShingle	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability Circumsporozoite like antigen vaccine Freund adjuvant Neutralizing antibody Peptide vaccine Protozoal vaccine Sodium chloride Sporozoite surface antigen 15 vaccine Unclassified drug Parasite antigen Peptide Protozoal dna Protozoal protein Protozoal vaccine Protozoon antibody Animal experiment Antibody detection Antibody production Antibody response Article Bioinformatics Cell culture Controlled study Cryptosporidiosis Cryptosporidium parvum Drug formulation Drug synthesis Enzyme linked immunosorbent assay Female Humoral immunity Immunological procedures In vitro study In vivo study Mouse Nonhuman Nucleotide sequence Peptide analysis Priority journal Seroneutralization test Swiss webster mouse Vaccine immunogenicity Chemistry Immunology Synthesis Cryptosporidium parvum Peptides Protozoan proteins Protozoan vaccines Cp15 Cryptosporidiosis Cryptosporidium Csl Peptide Vaccine protozoan protozoan protozoan Antibodies Antigens Dna
title_short	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
title_full	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
title_fullStr	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
title_full_unstemmed	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
title_sort	Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
dc.subject.keyword.spa.fl_str_mv	Circumsporozoite like antigen vaccine Freund adjuvant Neutralizing antibody Peptide vaccine Protozoal vaccine Sodium chloride Sporozoite surface antigen 15 vaccine Unclassified drug Parasite antigen Peptide Protozoal dna Protozoal protein Protozoal vaccine Protozoon antibody Animal experiment Antibody detection Antibody production Antibody response Article Bioinformatics Cell culture Controlled study Cryptosporidiosis Cryptosporidium parvum Drug formulation Drug synthesis Enzyme linked immunosorbent assay Female Humoral immunity Immunological procedures In vitro study In vivo study Mouse Nonhuman Nucleotide sequence Peptide analysis Priority journal Seroneutralization test Swiss webster mouse Vaccine immunogenicity Chemistry Immunology Synthesis Cryptosporidium parvum Peptides Protozoan proteins Protozoan vaccines Cp15 Cryptosporidiosis Cryptosporidium Csl Peptide Vaccine
topic	Circumsporozoite like antigen vaccine Freund adjuvant Neutralizing antibody Peptide vaccine Protozoal vaccine Sodium chloride Sporozoite surface antigen 15 vaccine Unclassified drug Parasite antigen Peptide Protozoal dna Protozoal protein Protozoal vaccine Protozoon antibody Animal experiment Antibody detection Antibody production Antibody response Article Bioinformatics Cell culture Controlled study Cryptosporidiosis Cryptosporidium parvum Drug formulation Drug synthesis Enzyme linked immunosorbent assay Female Humoral immunity Immunological procedures In vitro study In vivo study Mouse Nonhuman Nucleotide sequence Peptide analysis Priority journal Seroneutralization test Swiss webster mouse Vaccine immunogenicity Chemistry Immunology Synthesis Cryptosporidium parvum Peptides Protozoan proteins Protozoan vaccines Cp15 Cryptosporidiosis Cryptosporidium Csl Peptide Vaccine protozoan protozoan protozoan Antibodies Antigens Dna
dc.subject.keyword.eng.fl_str_mv	protozoan protozoan protozoan Antibodies Antigens Dna
description	Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended. © 2018 Elsevier Ltd
publishDate	2018
dc.date.created.spa.fl_str_mv	2018
dc.date.accessioned.none.fl_str_mv	2020-05-26T00:02:14Z
dc.date.available.none.fl_str_mv	2020-05-26T00:02:14Z
dc.type.eng.fl_str_mv	article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv	Artículo
dc.identifier.doi.none.fl_str_mv	https://doi.org/10.1016/j.vaccine.2018.09.044
dc.identifier.issn.none.fl_str_mv	0264410X 13588745
dc.identifier.uri.none.fl_str_mv	https://repository.urosario.edu.co/handle/10336/23462
url	https://doi.org/10.1016/j.vaccine.2018.09.044 https://repository.urosario.edu.co/handle/10336/23462
identifier_str_mv	0264410X 13588745
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.citationEndPage.none.fl_str_mv	6710
dc.relation.citationIssue.none.fl_str_mv	No. 45
dc.relation.citationStartPage.none.fl_str_mv	6703
dc.relation.citationTitle.none.fl_str_mv	Vaccine
dc.relation.citationVolume.none.fl_str_mv	Vol. 36
dc.relation.ispartof.spa.fl_str_mv	Vaccine, ISSN:0264410X, 13588745, Vol.36, No.45 (2018); pp. 6703-6710
dc.relation.uri.spa.fl_str_mv	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053915548&doi=10.1016%2fj.vaccine.2018.09.044&partnerID=40&md5=99a8717593b22d1e9b7987722393073d
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv	Abierto (Texto Completo)
rights_invalid_str_mv	Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv	application/pdf
dc.publisher.spa.fl_str_mv	Elsevier Ltd
institution	Universidad del Rosario
dc.source.instname.spa.fl_str_mv	instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv	reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv	Repositorio institucional EdocUR
repository.mail.fl_str_mv	edocur@urosario.edu.co
_version_	1814167479271817216

Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability

Publicaciones similares