Simultaneous assessment of rotavirus-specific memory B cells and serological memory after B cell depletion therapy with rituximab
The mechanisms that contribute to the maintenance of serological memory are still unclear. Rotavirus (RV) memory B cells (mBc) are enriched in IgM + and CD27- subpopulations, which are associated with autoimmune diseases pathogenesis. In patients with autoimmune diseases treated with Rituximab (RTX)...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2014
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/18703
- Acceso en línea:
- https://doi.org/10.1371/journal.pone.0097087
http://repository.urosario.edu.co/handle/10336/18703
- Palabra clave:
- Tetanus Toxoid
Rheumatoid Factor
Methylprednisolone
Middle Aged
Monoclonal Antibody
Specificity
Species
Rotavirus
Lymphocyte Depletion
Immunologic Memory
Immunoglobulin M
B-Lymphocytes
B-Lymphocyte Subsets
Autoantigens
Antibodies
Aged
Species Difference
Rotavirus
Procedures
Lymphocyte Depletion
Drug Effects
Systemic Lupus Erythematosus
Rheumatoid Arthritis
Nephelometry
Erythematosus Nephritis
Kinetic Nephelometry
Lupus
Cd27 Antigen
Double Stranded Dna Antibody
Rituximab
Antigen Specificity
Flow Cytometry
Enzyme Linked Immunosorbent Assay
Autoimmune Thrombocytopenia
Monoclonal
Antiphospholipid Syndrome
Immunoglobulin Blood Level
Female
Humans
Middle Aged
Rotavirus
Linfocitos B
Rituximab
- Rights
- License
- Abierto (Texto Completo)
Summary: | The mechanisms that contribute to the maintenance of serological memory are still unclear. Rotavirus (RV) memory B cells (mBc) are enriched in IgM + and CD27- subpopulations, which are associated with autoimmune diseases pathogenesis. In patients with autoimmune diseases treated with Rituximab (RTX), some autoantibodies (auto-Abs) decrease after treatment, but other auto-Abs and pathogen-specific IgG Abs remain unchanged. Thus, maintenance of autoimmune and pathogenspecific serological memory may depend on the type of antigen and/or Ab isotype evaluated. Antigen-specific mBc and antigen-specific Abs of different isotypes have not been simultaneously assessed in patients after RTX treatment. To study the relationship between mBc subpopulations and serological memory we characterized total, RV- and tetanus toxoid (TT)-specific mBc by flow cytometry in patients with autoimmune diseases before and after treatment with RTX. We also measured total, RV- and TT-Abs, and some auto-Abs by kinetic nephelometry, ELISA, and EliA tests, respectively. Minor differences were observed between the relative frequencies of RV-mBc in healthy controls and patients with autoimmune disease. After RTX treatment, naïve Bc and total, RV- and TT-specific mBc [IgM+, switched (IgA+/IgG+), IgM+ only, IgD+ only, and CD27- (IgA+/IgG+/IgM+)] were significantly diminished. An important decrease in total plasma IgM and minor decreases in total IgG and IgA levels were also observed. IgM rheumatoid factor, IgG anti-CCP, and IgG anti-dsDNA were significantly diminished. In contrast, RV-IgA, RV-IgG and RV-IgG1, and TT-IgG titers remained stable. In conclusion, in patients with autoimmunity, serological memory against RV and TT seem to be maintained by long-lived plasma cells, unaffected by RTX, and an important proportion of total IgM and serological memory against some auto-antigens seem to be maintained by short-lived plasma cells, dependent on mBc precursors depleted by RTX. © 2014 Herrera et al. |
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