Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia

Objective: To determine the molecular epidemiology and presence of virulence genes in communityacquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infectio...

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Tipo de recurso:
Fecha de publicación:
2013
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26027
Acceso en línea:
https://doi.org/10.1016/j.ijid.2013.02.029
https://repository.urosario.edu.co/handle/10336/26027
Palabra clave:
Staphylococcus aureus
CA-MRSA
Antibiotic resistance
Enterotoxins
USA300
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Abierto (Texto Completo)
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dc.title.spa.fl_str_mv Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
dc.title.TranslatedTitle.spa.fl_str_mv Epidemiología molecular y caracterización de genes de virulencia de aislados de estafilococo aureus resistentes a la meticilina adquiridos en la comunidad y adquiridos en el hospital en Colombia
title Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
spellingShingle Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
Staphylococcus aureus
CA-MRSA
Antibiotic resistance
Enterotoxins
USA300
title_short Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
title_full Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
title_fullStr Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
title_full_unstemmed Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
title_sort Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia
dc.subject.keyword.spa.fl_str_mv Staphylococcus aureus
CA-MRSA
Antibiotic resistance
Enterotoxins
USA300
topic Staphylococcus aureus
CA-MRSA
Antibiotic resistance
Enterotoxins
USA300
description Objective: To determine the molecular epidemiology and presence of virulence genes in communityacquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCCmec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results: Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68% of the isolates were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p = 0.001, odds ratio 4.73) and a protective effect for eta (p = 0.018, odds ratio 0.33). Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCCmec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections. 2013 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
publishDate 2013
dc.date.created.spa.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:20:29Z
dc.date.available.none.fl_str_mv 2020-08-06T16:20:29Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.ijid.2013.02.029
dc.identifier.issn.none.fl_str_mv ISSN: 1201-9712
EISSN: 1878-3511
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26027
url https://doi.org/10.1016/j.ijid.2013.02.029
https://repository.urosario.edu.co/handle/10336/26027
identifier_str_mv ISSN: 1201-9712
EISSN: 1878-3511
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv e749
dc.relation.citationIssue.none.fl_str_mv No. 9
dc.relation.citationStartPage.none.fl_str_mv e744
dc.relation.citationTitle.none.fl_str_mv International Journal of Infectious Diseases
dc.relation.citationVolume.none.fl_str_mv Vol. 17
dc.relation.ispartof.spa.fl_str_mv International Journal of Infectious Diseases, ISSN: 1201-9712;EISSN: 1878-3511, Vol.17, No.9 (September, 2013); pp.e744-e749
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dc.publisher.spa.fl_str_mv International Society for Infectious Diseases
Elsevier
dc.source.spa.fl_str_mv International Journal of Infectious Diseases
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
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spelling 24bdfa39-e0e4-446d-9946-8ec2d43e893e-181243ee7-0887-4fdf-929a-85db217fe41a-1549e9810-fa22-4f51-86f5-5ed62f9056fb-1bddac9f3-83ed-46e6-bfd8-78761785beb5-1f0157ec9-e439-4dd1-9860-97260069ae71-1108c1c04-f943-4436-aa72-718a7c1e5860-13c05de6c-30ca-4edb-a0be-6de7b5e0f092-179457397600d591e38d-35e3-48dc-bb3a-c007bd03c05e-1688f3641-bf02-4d3b-8743-b98aabf68ec8-1b2115e68-8bf3-4323-ba8c-b3cdae11c5aa-12020-08-06T16:20:29Z2020-08-06T16:20:29Z2013Objective: To determine the molecular epidemiology and presence of virulence genes in communityacquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCCmec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results: Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68% of the isolates were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p = 0.001, odds ratio 4.73) and a protective effect for eta (p = 0.018, odds ratio 0.33). Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCCmec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections. 2013 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.application/pdfhttps://doi.org/10.1016/j.ijid.2013.02.029ISSN: 1201-9712EISSN: 1878-3511https://repository.urosario.edu.co/handle/10336/26027engInternational Society for Infectious DiseasesElseviere749 No. 9e744International Journal of Infectious DiseasesVol. 17International Journal of Infectious Diseases, ISSN: 1201-9712;EISSN: 1878-3511, Vol.17, No.9 (September, 2013); pp.e744-e749https://www.ijidonline.com/action/showPdf?pii=S1201-9712%2813%2900122-7Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2International Journal of Infectious Diseasesinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURStaphylococcus aureusCA-MRSAAntibiotic resistanceEnterotoxinsUSA300Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in ColombiaEpidemiología molecular y caracterización de genes de virulencia de aislados de estafilococo aureus resistentes a la meticilina adquiridos en la comunidad y adquiridos en el hospital en ColombiaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Chavarro Portillo, BibianaMoreno, Jaime EnriqueYomayusa, NancyAlvarez, Carlos ArturoCastro Cardozo, Betsy EsperanzaEscobar Pérez, Javier AntonioDíaz, Paula LuciaIbáñez Pinilla, MilcíadesMendez-Alvarez, SebastiánLeal, Aura LuciaVanegas Gómez, NatashaORIGINALPIIS1201971213001227.pdfapplication/pdf619240https://repository.urosario.edu.co/bitstreams/f2fb5e6d-4864-43ad-8e59-d4cb8b628ada/download60be0c772305b5c8fc9a96a123e075c8MD51TEXTPIIS1201971213001227.pdf.txtPIIS1201971213001227.pdf.txtExtracted texttext/plain35776https://repository.urosario.edu.co/bitstreams/b58f7217-21b7-4b7e-9d55-24c423aff1b2/downloadb1ce91c4d359e0e4c9c7716093d26e81MD52THUMBNAILPIIS1201971213001227.pdf.jpgPIIS1201971213001227.pdf.jpgGenerated Thumbnailimage/jpeg4877https://repository.urosario.edu.co/bitstreams/9a177f65-c709-4531-a393-c99dda3a860f/download1011594d0964e42aa93d270e59b1e493MD5310336/26027oai:repository.urosario.edu.co:10336/260272021-06-03 00:50:24.198https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co