Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
Background Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2011
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/27766
- Acceso en línea:
- https://doi.org/10.1186/1471-2164-12-182
https://repository.urosario.edu.co/handle/10336/27766
- Palabra clave:
- Conjugate linoleic acid
Connective tissue growth factor
Mesenteric lymph node
Conjugate linoleic acid isomer
Suckling period
- Rights
- License
- Abierto (Texto Completo)
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70f6c48d-d7ae-436a-81f3-dc956dcf0187b1a11603-1780-430d-b877-00ae151ab80137126581-ba07-499a-b785-14b322dfae665248352660027331a50-730b-4136-9048-9032a7d852ce9a8e379d-d38d-49bb-b106-ae5949a9195b32736bb1-c609-4e39-8856-4656d8383249f0211e1d-8491-4eff-9a14-64b3fdade0e0167a9aeb-e599-4e58-81d7-ad7baffaf9d72020-08-19T14:43:46Z2020-08-19T14:43:46Z2011-04-11Background Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life. Results The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip® Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR. Conclusions Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.application/pdfhttps://doi.org/10.1186/1471-2164-12-182EISSN: 1471-2164https://repository.urosario.edu.co/handle/10336/27766engBioMed CentralNo. 182BMC GenomicsVol. 12BMC Genomics, EISSN: 1471-2164, Vol.12, No.182 (2011); 13 pp.https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/1471-2164-12-182Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2BMC Genomicsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURConjugate linoleic acidConnective tissue growth factorMesenteric lymph nodeConjugate linoleic acid isomerSuckling periodGene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and sucklingPerfiles de expresión génica en los ganglios linfáticos mesentéricos de rata tras la suplementación con ácido linoleico conjugado durante la gestación y la lactanciaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Selga, ElisabetPérez-Cano, Francisco JFranch, ÀngelsRamírez Santana, Heily CarolinaRivero, MontserratCiudad, Carlos JCastellote,CristinaNoé, VéroniqueORIGINAL1471-2164-12-182.pdfapplication/pdf1072429https://repository.urosario.edu.co/bitstreams/548624a1-81d1-4cfb-9207-3094efb2b3a1/downloadf07353bad6f16ff8a5461e27d89745e3MD51TEXT1471-2164-12-182.pdf.txt1471-2164-12-182.pdf.txtExtracted texttext/plain61757https://repository.urosario.edu.co/bitstreams/dce4c134-6412-4c99-8ee6-a98e92056786/downloadddfd3568f30ce0e41977fcdc1ae73c70MD52THUMBNAIL1471-2164-12-182.pdf.jpg1471-2164-12-182.pdf.jpgGenerated Thumbnailimage/jpeg4558https://repository.urosario.edu.co/bitstreams/bdba9bfc-f047-4c40-8db7-307604569768/downloadd52ae973ec48314d0be4f9157698f61fMD5310336/27766oai:repository.urosario.edu.co:10336/277662021-10-06 23:03:32.522https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
dc.title.TranslatedTitle.spa.fl_str_mv |
Perfiles de expresión génica en los ganglios linfáticos mesentéricos de rata tras la suplementación con ácido linoleico conjugado durante la gestación y la lactancia |
title |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
spellingShingle |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling Conjugate linoleic acid Connective tissue growth factor Mesenteric lymph node Conjugate linoleic acid isomer Suckling period |
title_short |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
title_full |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
title_fullStr |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
title_full_unstemmed |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
title_sort |
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling |
dc.subject.keyword.spa.fl_str_mv |
Conjugate linoleic acid Connective tissue growth factor Mesenteric lymph node Conjugate linoleic acid isomer Suckling period |
topic |
Conjugate linoleic acid Connective tissue growth factor Mesenteric lymph node Conjugate linoleic acid isomer Suckling period |
description |
Background Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life. Results The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip® Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR. Conclusions Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life. |
publishDate |
2011 |
dc.date.created.spa.fl_str_mv |
2011-04-11 |
dc.date.accessioned.none.fl_str_mv |
2020-08-19T14:43:46Z |
dc.date.available.none.fl_str_mv |
2020-08-19T14:43:46Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1186/1471-2164-12-182 |
dc.identifier.issn.none.fl_str_mv |
EISSN: 1471-2164 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/27766 |
url |
https://doi.org/10.1186/1471-2164-12-182 https://repository.urosario.edu.co/handle/10336/27766 |
identifier_str_mv |
EISSN: 1471-2164 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 182 |
dc.relation.citationTitle.none.fl_str_mv |
BMC Genomics |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 12 |
dc.relation.ispartof.spa.fl_str_mv |
BMC Genomics, EISSN: 1471-2164, Vol.12, No.182 (2011); 13 pp. |
dc.relation.uri.spa.fl_str_mv |
https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/1471-2164-12-182 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
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Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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BioMed Central |
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BMC Genomics |
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Universidad del Rosario |
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