Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling

Background Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out...

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Fecha de publicación:
2011
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27766
Acceso en línea:
https://doi.org/10.1186/1471-2164-12-182
https://repository.urosario.edu.co/handle/10336/27766
Palabra clave:
Conjugate linoleic acid
Connective tissue growth factor
Mesenteric lymph node
Conjugate linoleic acid isomer
Suckling period
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spelling 70f6c48d-d7ae-436a-81f3-dc956dcf0187b1a11603-1780-430d-b877-00ae151ab80137126581-ba07-499a-b785-14b322dfae665248352660027331a50-730b-4136-9048-9032a7d852ce9a8e379d-d38d-49bb-b106-ae5949a9195b32736bb1-c609-4e39-8856-4656d8383249f0211e1d-8491-4eff-9a14-64b3fdade0e0167a9aeb-e599-4e58-81d7-ad7baffaf9d72020-08-19T14:43:46Z2020-08-19T14:43:46Z2011-04-11Background Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life. Results The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip® Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR. Conclusions Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.application/pdfhttps://doi.org/10.1186/1471-2164-12-182EISSN: 1471-2164https://repository.urosario.edu.co/handle/10336/27766engBioMed CentralNo. 182BMC GenomicsVol. 12BMC Genomics, EISSN: 1471-2164, Vol.12, No.182 (2011); 13 pp.https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/1471-2164-12-182Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2BMC Genomicsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURConjugate linoleic acidConnective tissue growth factorMesenteric lymph nodeConjugate linoleic acid isomerSuckling periodGene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and sucklingPerfiles de expresión génica en los ganglios linfáticos mesentéricos de rata tras la suplementación con ácido linoleico conjugado durante la gestación y la lactanciaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Selga, ElisabetPérez-Cano, Francisco JFranch, ÀngelsRamírez Santana, Heily CarolinaRivero, MontserratCiudad, Carlos JCastellote,CristinaNoé, VéroniqueORIGINAL1471-2164-12-182.pdfapplication/pdf1072429https://repository.urosario.edu.co/bitstreams/548624a1-81d1-4cfb-9207-3094efb2b3a1/downloadf07353bad6f16ff8a5461e27d89745e3MD51TEXT1471-2164-12-182.pdf.txt1471-2164-12-182.pdf.txtExtracted texttext/plain61757https://repository.urosario.edu.co/bitstreams/dce4c134-6412-4c99-8ee6-a98e92056786/downloadddfd3568f30ce0e41977fcdc1ae73c70MD52THUMBNAIL1471-2164-12-182.pdf.jpg1471-2164-12-182.pdf.jpgGenerated Thumbnailimage/jpeg4558https://repository.urosario.edu.co/bitstreams/bdba9bfc-f047-4c40-8db7-307604569768/downloadd52ae973ec48314d0be4f9157698f61fMD5310336/27766oai:repository.urosario.edu.co:10336/277662021-10-06 23:03:32.522https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
dc.title.TranslatedTitle.spa.fl_str_mv Perfiles de expresión génica en los ganglios linfáticos mesentéricos de rata tras la suplementación con ácido linoleico conjugado durante la gestación y la lactancia
title Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
spellingShingle Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
Conjugate linoleic acid
Connective tissue growth factor
Mesenteric lymph node
Conjugate linoleic acid isomer
Suckling period
title_short Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
title_full Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
title_fullStr Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
title_full_unstemmed Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
title_sort Gene expression profiles in rat mesenteric lymph nodes upon supplementation with Conjugated Linoleic Acid during gestation and suckling
dc.subject.keyword.spa.fl_str_mv Conjugate linoleic acid
Connective tissue growth factor
Mesenteric lymph node
Conjugate linoleic acid isomer
Suckling period
topic Conjugate linoleic acid
Connective tissue growth factor
Mesenteric lymph node
Conjugate linoleic acid isomer
Suckling period
description Background Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life. Results The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip® Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR. Conclusions Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.
publishDate 2011
dc.date.created.spa.fl_str_mv 2011-04-11
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:43:46Z
dc.date.available.none.fl_str_mv 2020-08-19T14:43:46Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1186/1471-2164-12-182
dc.identifier.issn.none.fl_str_mv EISSN: 1471-2164
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27766
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https://repository.urosario.edu.co/handle/10336/27766
identifier_str_mv EISSN: 1471-2164
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 182
dc.relation.citationTitle.none.fl_str_mv BMC Genomics
dc.relation.citationVolume.none.fl_str_mv Vol. 12
dc.relation.ispartof.spa.fl_str_mv BMC Genomics, EISSN: 1471-2164, Vol.12, No.182 (2011); 13 pp.
dc.relation.uri.spa.fl_str_mv https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/1471-2164-12-182
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dc.publisher.spa.fl_str_mv BioMed Central
dc.source.spa.fl_str_mv BMC Genomics
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