Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)

Blastocystis es un microeucariota intestinal que ha suscitado atención debido a su amplia distribución en animales y seres humanos. El riesgo de circulación zoonótica surge principalmente del contacto estrecho con animales infectados. Por lo tanto, el siguiente estudio tuvo como objetivo evaluar la...

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Fecha de publicación:: 2023

Institución:: Universidad del Rosario

Repositorio:: Repositorio EdocUR - U. Rosario

Idioma:: eng

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network_acronym_str	EDOCUR2
network_name_str	Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.none.fl_str_mv	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
dc.title.TranslatedTitle.none.fl_str_mv	Blastocystis genetic diversity in animal and human samples from different departments of Colombia using complete sequencing of the 18S rRNA gene (SSU rRNA) by Oxford Nanopore Technologies (ONT)
title	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
spellingShingle	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT) Blastocystis Subtipos STs Colombia Animales Humanos SSU ARNr Secuenciación Blastocystis Subtypes STs Colombia Animals Humans SSU rRNA Sequencing
title_short	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
title_full	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
title_fullStr	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
title_full_unstemmed	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
title_sort	Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)
dc.contributor.advisor.none.fl_str_mv	Ramírez González, Juan David Muñoz Díaz, Claudia Marina
dc.contributor.gruplac.none.fl_str_mv	Grupo de Investigaciones Microbiológicas UR (GIMUR)
dc.subject.none.fl_str_mv	Blastocystis Subtipos STs Colombia Animales Humanos SSU ARNr Secuenciación
topic	Blastocystis Subtipos STs Colombia Animales Humanos SSU ARNr Secuenciación Blastocystis Subtypes STs Colombia Animals Humans SSU rRNA Sequencing
dc.subject.keyword.none.fl_str_mv	Blastocystis Subtypes STs Colombia Animals Humans SSU rRNA Sequencing
description	Blastocystis es un microeucariota intestinal que ha suscitado atención debido a su amplia distribución en animales y seres humanos. El riesgo de circulación zoonótica surge principalmente del contacto estrecho con animales infectados. Por lo tanto, el siguiente estudio tuvo como objetivo evaluar la diversidad y frecuencia de los subtipos de Blastocystis en muestras humanas y animales colombianas utilizando la secuenciación completa del gen 18S rRNA. Para este propósito, 341 muestras fecales humanas y 277 muestras fecales animales (de bovinos, ovinos, caprinos, cerdos, gatos y perros), fueron recolectadas de diferentes regiones colombianas y analizadas usando detección basada en PCR y secuenciación de próxima generación (NGS) del gen 18S SSU rRNA de longitud completa. Entre las 618 muestras de ambos huéspedes, humanos y animales, los resultados revelaron una frecuencia generalizada de Blastocystis, con un 48,09% (n=164) en humanos y un 31,4% (n=87) de detección en animales. Perros, gatos, ovejas, cerdos y animales salvajes dieron positivo, en consonancia con los patrones de prevalencia mundial. Además, se secuenciaron 29 muestras humanas y 23 muestras animales mediante tecnología ONT, a partir de las cuales se generaron 11 secuencias únicas de lectura larga que se agruparon con sus secuencias de referencia comparadas. La distribución de subtipos varió dentro de los hospedadores, detectándose ST1 y ST3 tanto en muestras humanas como animales. Los subtipos ST5, ST10, ST14, ST15, ST21, ST24, ST25 y ST26 se limitaron a hospedadores animales, algunos de los cuales se considera que tienen potencial zoonótico. Por otra parte, el ST2 se encontró exclusivamente en muestras humanas de la región de Bolívar. Se produjeron infecciones mixtas tanto en animales como en humanos, 60,86% y 27,58% respectivamente. Además, hasta donde sabemos, este es el primer estudio en Colombia que identifica ST15 en cerdos y ST25 en ovejas. Los subtipos (STs) identificados en este estudio indican que ciertos animales pueden servir como reservorios con potencial de transmisión zoonótica. La identificación de subtipos zoonóticos pone de relieve el uso de la secuenciación de nueva generación, ya que la profundidad y la resolución de las secuencias aumentan, lo que permite comprender mejor los ST de importancia médica y veterinaria. También revela la coexistencia de diversos subtipos entre hospedadores. Es esencial seguir investigando para comprender la dinámica de transmisión, las implicaciones sanitarias y las estrategias de detección de Blastocystis en animales y humanos, sobre todo por el papel de los animales como reservorios y su estrecha interacción con los humanos.
publishDate	2023
dc.date.created.none.fl_str_mv	2023-11-29
dc.date.accessioned.none.fl_str_mv	2024-05-08T20:35:38Z
dc.date.available.none.fl_str_mv	2024-05-08T20:35:38Z
dc.type.none.fl_str_mv	bachelorThesis
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_7a1f
dc.type.document.none.fl_str_mv	Artículo
dc.type.spa.none.fl_str_mv	Artículo
dc.identifier.doi.none.fl_str_mv	https://doi.org/10.1016/j.actatropica.2023.107090
dc.identifier.uri.none.fl_str_mv	https://repository.urosario.edu.co/handle/10336/42536
url	https://doi.org/10.1016/j.actatropica.2023.107090 https://repository.urosario.edu.co/handle/10336/42536
dc.language.iso.none.fl_str_mv	eng
language	eng
dc.rights.*.fl_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.none.fl_str_mv	Abierto (Texto Completo)
dc.rights.economicrights.none.fl_str_mv	Universidad del Rosario
dc.rights.uri.*.fl_str_mv	http://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv	Attribution-NonCommercial-NoDerivatives 4.0 International Abierto (Texto Completo) Universidad del Rosario http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2
dc.format.extent.none.fl_str_mv	49 pp
dc.format.mimetype.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Universidad del Rosario
dc.publisher.department.none.fl_str_mv	Facultad de Ciencias Naturales
dc.publisher.program.none.fl_str_mv	Maestría en Ciencias Naturales
publisher.none.fl_str_mv	Universidad del Rosario
institution	Universidad del Rosario
dc.source.bibliographicCitation.none.fl_str_mv	Ajjampur, Sitara SR; Tan, Kevin SW (2016) Pathogenic mechanisms in Blastocystis spp.—Interpreting results from in vitro and in vivo studies. En: Parasitology international. Vol. 65; No. 6; pp. 772 - 779; 1383-5769; Alfellani, Mohammed A; Stensvold, C Rune; Vidal-Lapiedra, Amparo; Onuoha, Emeh Sunday Uche; Fagbenro-Beyioku, Adetayo F; Clark, C Graham (2013) Variable geographic distribution of Blastocystis subtypes and its potential implications. En: Acta tropica. Vol. 126; No. 1; pp. 11 - 18; 0001-706X; Alfellani, Mohammed A; Taner-Mulla, Derya; Jacob, Alison S; Imeede, Christine Atim; Yoshikawa, Hisao; Stensvold, C Rune; Clark, C Graham (2013) Genetic diversity of Blastocystis in livestock and zoo animals. En: Protist. Vol. 164; No. 4; pp. 497 - 509; 1434-4610; Barbosa, Carolina Valença; Batista, Rosemary de Jesus; Igreja, Ricardo Pereira; Levy, Claudia Masini d’Avila; Werneck de Macedo, Heloisa; Santos, Helena Lúcia Carneiro (2017) Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil. En: Parasites & Vectors, Vol 10, Iss 1, Pp 1-9 (2017). No. 1; pp. 1 1756-3305; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.97501ef7c2c5465092343ee6868b417e〈=es&site=eds-live&scope=site. Disponible en: 10.1186/s13071-017-2458-0. Casero, Rodolfo Daniel; Mongi, Florencia; Sánchez, Angie; Ramírez, Juan David (2015) Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation. En: Acta Tropica. Vol. 148; pp. 156 - 161; 0001-706X; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S0001706X15001382〈=es&site=eds-live&scope=site. Disponible en: 10.1016/j.actatropica.2015.05.004. Cian, Amandine; El Safadi, Dima; Osman, Marwan; Moriniere, Romain; Gantois, Nausicaa; Benamrouz-Vanneste, Sadia; Delgado-Viscogliosi, Pilar; Guyot, Karine; Li, Luen-Luen; Monchy, Sébastien (2017) Molecular epidemiology of Blastocystis sp. in various animal groups from two French zoos and evaluation of potential zoonotic risk. En: PloS one. Vol. 12; No. 1; pp. e0169659 1932-6203; Deng, Lei; Wojciech, Lukasz; Gascoigne, Nicholas RJ; Peng, Guangneng; Tan, Kevin SW (2021) New insights into the interactions between Blastocystis, the gut microbiota, and host immunity. En: PLoS Pathogens. Vol. 17; No. 2; pp. e1009253 1553-7366; Gabrielli, S.; Furzi, F.; Fontanelli Sulekova, L.; Taliani, G.; Mattiucci, S. (2020) Occurrence of Blastocystis-subtypes in patients from Italy revealed association of ST3 with a healthy gut microbiota. En: Parasite Epidemiol Control. Vol. 9; pp. e00134 2405-6731; Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/32258445. Disponible en: 10.1016/j.parepi.2020.e00134. Higuera, Adriana; Herrera, Giovanny; Jimenez, Paula; García-Corredor, Diego; Pulido-Medellín, Martin; Bulla-Castañeda, Diana M; Pinilla, Juan Carlos; Moreno-Pérez, Darwin A; Maloney, Jenny G; Santín, Mónica (2021) Identification of multiple Blastocystis subtypes in domestic animals from Colombia using amplicon-based next generation sequencing. En: Frontiers in veterinary science. pp. 932 2297-1769; Higuera, Adriana; Muñoz, Marina; López, Myriam Consuelo; Reyes, Patricia; Urbano, Plutarco; Villalobos, Oswaldo; Ramírez, Juan David (2020) Succinate dehydrogenase gene as a marker for studying Blastocystis genetic diversity. En: Heliyon. Vol. 6; No. 11; pp. e05387 2405-8440; Hublin, Josephine SY; Maloney, Jenny G; Santin, Monica (2020) Blastocystis in domesticated and wild mammals and birds. En: Research in Veterinary Science. 0034-5288; Iebba, V.; Santangelo, F.; Totino, V.; Pantanella, F.; Monsia, A.; Di Cristanziano, V.; Di Cave, D.; Schippa, S.; Berrilli, F.; D'Alfonso, R. (2016) Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d'Ivoire. En: J Infect Dev Ctries. Vol. 10; No. 9; pp. 1035 - 1041; 1972-2680; Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/27694739. Disponible en: 10.3855/jidc.8179. Jiménez, Paula Andrea; Jaimes, Jesús Eduardo; Ramírez, Juan David (2019) A summary of Blastocystis subtypes in North and South America. En: Parasites & vectors. Vol. 12; No. 1; pp. 1 - 9; 1756-3305; Lee, Li; Chye, Tan; Karmacharya, Biraj; Govind, Suresh (2012) Blastocystis sp.: waterborne zoonotic organism, a possibility?. En: Parasites & Vectors, Vol 5, Iss 1, p 130 (2012). No. 1; pp. 130 1756-3305; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.f031f489a68c49b5a34b86d9c081a859〈=es&site=eds-live&scope=site. Disponible en: 10.1186/1756-3305-5-130. Li, Lan-Hua; Zhou, Xiao-Nong; Du, Zun-Wei; Wang, Xue-Zhong; Wang, Li-Bo; Jiang, Jin-Yong; Yoshikawa, Hisao; Steinmann, Peter; Utzinger, Jürg; Wu, Zhiliang; Chen, Jia-Xu; Chen, Shao-Hong; Zhang, Ling (2007) Molecular epidemiology of human Blastocystis in a village in Yunnan province, China. En: Parasitology International. Vol. 56; No. 4; pp. 281 - 286; 1383-5769; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=cmedm&AN=17627869〈=es&site=eds-live&scope=site. Londoño-Franco, Ángela Liliana; Loaiza-Herrera, Juliana; Lora-Suárez, Fabiana María; Gómez-Marín, Jorge Enrique (2014) Frecuencia y fuentes de Blastocystis sp . en niños de 0 a 5 años de edad atendidos en hogares infantiles públicos de la zona urbana de Calarcá, Colombia / Blastocystis sp . frequency and sources among children from 0 to 5 years of age attending public day care centers in Calarcá, Colombia. En: Biomédica. No. 2; pp. 218 0120-4157; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edssci&AN=edssci.S0120.41572014000200008〈=es&site=eds-live&scope=site. Disponible en: 10.7705/biomedica.v34i2.2124. Maloney, Jenny G; da Cunha, Maria JR; Molokin, Aleksey; Cury, Márcia C; Santin, Monica (2021) Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes. En: Parasitology Research. pp. 1 - 13; 1432-1955; Maloney, Jenny G; Jang, Yunah; Molokin, Aleksey; George, Nadja S; Santin, Monica (2021) Wide Genetic Diversity of Blastocystis in White-Tailed Deer (Odocoileus virginianus) from Maryland, USA. En: Microorganisms. Vol. 9; No. 6; pp. 1343 Maloney, Jenny G; Molokin, Aleksey; da Cunha, Maria Júlia Rodrigues; Cury, Márcia Cristina; Santin, Monica (2020) Blastocystis subtype distribution in domestic and captive wild bird species from Brazil using next generation amplicon sequencing. En: Parasite epidemiology and control. Vol. 9; pp. e00138 2405-6731; Maloney, Jenny G; Molokin, Aleksey; Santin, Monica (2019) Next generation amplicon sequencing improves detection of Blastocystis mixed subtype infections. En: Infection, Genetics and Evolution. Vol. 73; pp. 119 - 125; 1567-1348; Maloney, Jenny G; Molokin, Aleksey; Santin, Monica (2020) Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene. En: Parasites & vectors. Vol. 13; No. 1; pp. 1 - 8; 1756-3305; Maloney, Jenny G; Santin, Monica (2021) Mind the gap: New full-length sequences of Blastocystis subtypes generated via Oxford Nanopore Minion sequencing allow for comparisons between full-length and partial sequences of the small subunit of the ribosomal RNA gene. En: Microorganisms. Vol. 9; No. 5; pp. 997 Maloney, Jenny G.; Lombard, Jason E.; Urie, Natalie J.; Shivley, Chelsey B.; Santin, Monica (2018) Zoonotic and genetically diverse subtypes of Blastocystis in US pre-weaned dairy heifer calves. En: Parasitology Research. Vol. 2; No. 118; pp. 575 - 582; 1432-1955; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=cmedm&AN=30483890〈=es&site=eds-live&scope=site. Disponible en: 10.1007/s00436-018-6149-3. Ministerio de Salud y Protección Social (2015) Encuesta Nacional de Parasitismo Intestinal en Población Escolar, Colombia 2012. : Facultad Nacional Salud Pública, Universidad de Antioquia; Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/encuesta-nacional-de-parasitismo-2012-2014.pdf. Peña, S.; Carrasco, G.; Rojas, P.; Castillo, D.; Ozaki, L. S.; Mercado, R. (2020) Determination of subtypes of Blastocystis sp. in Chilean patients with and without inflammatory bowel syndrome, A preliminary report. En: Parasite Epidemiol Control. Vol. 8; pp. e00125 2405-6731; Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/31890923. Disponible en: 10.1016/j.parepi.2019.e00125. Peña, S.; Carrasco, G.; Rojas, P.; Gotteland, M.; Castillo, D.; Ozaki, L. S.; Mercado, R. (2019) Diferent Subtypes of Blastocystis hominis (ST1, ST2 and ST4) are present in Chilean patients with Inflamatory Bowel Disease. Qi, Meng; Wei, Zilin; Zhang, Ying; Zhang, Qiyuan; Li, Juanfeng; Zhang, Longxian; Wang, Rongjun (2020) Genetic diversity of Blastocystis in kindergarten children in southern Xinjiang, China. En: Parasites & Vectors. Vol. 13; No. 1; pp. 1 - 6; 1756-3305; Ramirez, Juan David; Sanchez, Angie; Hernandez, Carolina; Florez, Carolina; Consuelo Bernal, Maria; Cesar Giraldo, Julio; Reyes, Patricia; Consuelo Lopez, Myriam; Garcia, Lineth; Cooper, Philip J.; Vicuna, Yosselin; Mongi, Florencia; Casero, Rodolfo D. (2016) Geographic distribution of human Blastocystis subtypes in South America. Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edswsc&AN=000375652800006〈=es&site=eds-live&scope=site. Ramírez, Juan David; Flórez, Carolina; Olivera, Mario; Bernal, María Consuelo; Giraldo, Julio Cesar (2017) Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia. En: PLoS ONE. Vol. 12; No. 2; pp. 1 - 13; 19326203; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=121368795〈=es&site=eds-live&scope=site. Disponible en: 10.1371/journal.pone.0172586. Roberts, Tamalee; Stark, Damien; Harkness, Jock; Ellis, John (2013) Subtype distribution of Blastocystis isolates from a variety of animals from New South Wales, Australia. En: Veterinary Parasitology. Vol. 196; No. 1-2; pp. 85 - 89; 0304-4017; Ruaux, G., Craig; Stang, Bernadette (2014) Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest. En: PLoS ONE, Vol 9, Iss 9, p e107496 (2014). No. 9; pp. e107496 1932-6203; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.921334fb092043a6a8c7c8f27e64d73a〈=es&site=eds-live&scope=site. Disponible en: 10.1371/journal.pone.0107496. Stensvold, C. Rune; Alfellani, Mohammed; Clark, C. Graham (2012) Levels of genetic diversity vary dramatically between Blastocystis subtypes. En: Infection, Genetics and Evolution. Vol. 12; pp. 263 - 273; 1567-1348; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S156713481100387X〈=es&site=eds-live&scope=site. Disponible en: 10.1016/j.meegid.2011.11.002. Stensvold, C. Rune; Clark, C. Graham (2016) Current status of Blastocystis: A personal view. En: Parasitology International. Vol. 65; No. Part B; pp. 763 - 771; 1383-5769; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S1383576916301544〈=es&site=eds-live&scope=site. Disponible en: 10.1016/j.parint.2016.05.015. Stensvold, Christen Rune (2013) Blastocystis: Genetic diversity and molecular methods for diagnosis and epidemiology. En: Tropical Parasitology. Vol. 3; No. 1; pp. 26 - 34; 22295070; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=89188102〈=es&site=eds-live&scope=site. Disponible en: 10.4103/2229-5070.113896. Stensvold, Christen Rune; Ahmed, Umran Nisar; Andersen, Lee O'Brien; Nielsen, Henrik Vedel (2012) Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp. En: Journal of clinical microbiology. Vol. 50; No. 6; pp. 1847 - 1851; 0095-1137; Stensvold, Christen Rune; Clark, C Graham (2020) Pre-empting Pandora’s box: Blastocystis subtypes revisited. En: Trends in parasitology. Vol. 36; No. 3; pp. 229 - 232; 1471-4922; Stensvold, Christen Rune; Tan, Kevin SW; Clark, C Graham (2020) Blastocystis. En: Trends in parasitology. Vol. 36; No. 3; pp. 315 - 316; 1471-4922; Vega, Laura; Herrera, Giovanny; Muñoz, Marina; Patarroyo, Manuel A; Maloney, Jenny G; Santín, Monica; Ramírez, Juan David (2021) Gut microbiota profiles in diarrheic patients with co-occurrence of Clostridioides difficile and Blastocystis. En: PloS one. Vol. 16; No. 3; pp. e0248185 1932-6203; Wawrzyniak, Ivan; Poirier, Philippe; Viscogliosi, Eric; Dionigia, Meloni; Texier, Catherine; Delbac, Frédéric; Alaoui, Hicham El (2013) Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis. En: Therapeutic advances in infectious disease. Vol. 1; No. 5; pp. 167 - 178; 2049-9361; Yoshikawa, Hisao; Koyama, Yukiko; Tsuchiya, Erika; Takami, Kazutoshi (2016) Blastocystis phylogeny among various isolates from humans to insects. En: Parasitology international. Vol. 65; No. 6; pp. 750 - 759; 1383-5769; Zhao, GH; Hu, XF; Liu, TL; Hu, RS; Yu, ZQ; Yang, WB; Wu, YL; Yu, SK; Song, JK (2017) Molecular characterization of Blastocystis sp. in captive wild animals in Qinling Mountains. En: Parasitology research. Vol. 116; No. 8; pp. 2327 - 2333; 1432-1955; Osorio-Pulgarin, Maria I.; Higuera, Adriana; Beltran-Álzate, Juan C.; Sánchez-Jiménez, Miryan; Ramírez, Juan David (2021) Epidemiological and Molecular Characterization of Blastocystis Infection in Children Attending Daycare Centers in Medellín, Colombia. En: Biology. Vol. 10; No. 7; pp. 669 Consultado en: 2021/10/10/22:39:03. Disponible en: https://www.mdpi.com/2079-7737/10/7/669. Disponible en: 10.3390/biology10070669. Castañeda, Sergio; Muñoz, Marina; Villamizar, Ximena; Hernández, Paula C.; Vásquez, Luis Reinel; Tito, Raúl Yhossef; Ramírez, Juan David (2020) Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia. En: Parasites & Vectors. Vol. 13; No. 1; pp. 521 1756-3305; Consultado en: 2021/10/13/20:30:17. Disponible en: https://doi.org/10.1186/s13071-020-04392-9. Disponible en: 10.1186/s13071-020-04392-9. Higuera, Adriana; Villamizar, Ximena; Herrera, Giovanny; Giraldo, Julio Cesar; Vasquez-A, Luis Reinel; Urbano, Plutarco; Villalobos, Oswaldo; Tovar, Catalina; Ramírez, Juan David (2020) Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia. En: PeerJ. Vol. 8; pp. e8554 2167-8359; Consultado en: 2021/10/14/00:32:23. Disponible en: https://peerj.com/articles/8554. Disponible en: 10.7717/peerj.8554. Jain, Miten; Koren, Sergey; Miga, Karen H.; Quick, Josh; Rand, Arthur C.; Sasani, Thomas A.; Tyson, John R.; Beggs, Andrew D.; Dilthey, Alexander T.; Fiddes, Ian T.; Malla, Sunir; Marriott, Hannah; Nieto, Tom; O'Grady, Justin; Olsen, Hugh E.; Pedersen, Brent S.; Rhie, Arang; Richardson, Hollian; Quinlan, Aaron R.; Snutch, Terrance P.; Tee, Louise; Paten, Benedict; Phillippy, Adam M.; Simpson, Jared T.; Loman, Nicholas J.; Loose, Matthew (2018) Nanopore sequencing and assembly of a human genome with ultra-long reads. En: Nature Biotechnology. Vol. 36; No. 4; pp. 338 - 345; 1546-1696; Consultado en: 2021/10/20/22:10:21. Disponible en: https://www.nature.com/articles/nbt.4060. Disponible en: 10.1038/nbt.4060. Bowden, Rory; Davies, Robert W.; Heger, Andreas; Pagnamenta, Alistair T.; de Cesare, Mariateresa; Oikkonen, Laura E.; Parkes, Duncan; Freeman, Colin; Dhalla, Fatima; Patel, Smita Y.; Popitsch, Niko; Ip, Camilla L. C.; Roberts, Hannah E.; Salatino, Silvia; Lockstone, Helen; Lunter, Gerton; Taylor, Jenny C.; Buck, David; Simpson, Michael A.; Donnelly, Peter (2019) Sequencing of human genomes with nanopore technology. En: Nature Communications. Vol. 10; No. 1; pp. 1869 2041-1723; Consultado en: 2021/10/20/22:11:08. Disponible en: https://www.nature.com/articles/s41467-019-09637-5. Disponible en: 10.1038/s41467-019-09637-5. Katoh, Kazutaka; Standley, Daron M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. En: Molecular Biology and Evolution. Vol. 30; No. 4; pp. 772 - 780; 0737-4038; Consultado en: 2021/10/26/04:30:25. Disponible en: https://doi.org/10.1093/molbev/mst010. Disponible en: 10.1093/molbev/mst010. Minh, Bui Quang; Schmidt, Heiko A; Chernomor, Olga; Schrempf, Dominik; Woodhams, Michael D; von Haeseler, Arndt; Lanfear, Robert (2020) IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era. En: Molecular Biology and Evolution. Vol. 37; No. 5; pp. 1530 - 1534; 0737-4038; Consultado en: 2021/10/26/04:48:00. Disponible en: https://doi.org/10.1093/molbev/msaa015. Disponible en: 10.1093/molbev/msaa015. Letunic, Ivica; Bork, Peer (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. En: Nucleic Acids Research. Vol. 44; No. W1; pp. W242 - 245; 1362-4962; Disponible en: 10.1093/nar/gkw290. Cabrine-Santos, Marlene; Moura, Renata Gregório Franco; Pedrosa, André Luiz; Correia, Dalmo; Oliveira-Silva, Márcia Benedita de (2021) Molecular characterization of Blastocystis subtypes isolated in the city of Uberaba, Minas Gerais State, Brazil. En: Revista da Sociedade Brasileira de Medicina Tropical. Vol. 54; 0037-8682, 1678-9849; Consultado en: 2022/01/04/23:04:29. Disponible en: http://www.scielo.br/j/rsbmt/a/8MwpT4t5JZ6QWLsnrjrqCdr/?lang=en. Disponible en: 10.1590/0037-8682-0305-2021. Alzate, Juan F.; Toro-Londoño, Miguel; Cabarcas, Felipe; Garcia-Montoya, Gisela; Galvan-Diaz, Ana (2020) Contrasting microbiota profiles observed in children carrying either Blastocystis spp. or the commensal amoebas Entamoeba coli or Endolimax nana. En: Scientific Reports. Vol. 10; No. 1; pp. 15354 2045-2322; Consultado en: 2022/02/15/20:53:08. Disponible en: https://www.nature.com/articles/s41598-020-72286-y. Disponible en: 10.1038/s41598-020-72286-y. Yañez, Claudia Muñoz; Hernández, Alejandra Méndez; Sandoval, Alondra Martínez; Domínguez, María Aurora Maravilla; Muñiz, Soraya Amalí Zavaleta; Gómez, Janeth Oliva Guangorena (2021) Prevalence of Blastocystis and its association with Firmicutes/Bacteroidetes ratio in clinically healthy and metabolically ill subjects. En: BMC Microbiology. Vol. 21; No. 1; pp. 339 1471-2180; Consultado en: 2022/03/17/20:01:07. Disponible en: https://doi.org/10.1186/s12866-021-02402-z. Disponible en: 10.1186/s12866-021-02402-z. Nemati, Sara; Zali, Mohammad Reza; Johnson, Paul; Mirjalali, Hamed; Karanis, Panagiotis (2021) Molecular prevalence and subtype distribution of Blastocystis sp. in Asia and in Australia. En: Journal of Water and Health. Vol. 19; No. 5; pp. 687 - 704; 1477-8920; Consultado en: 2022/04/02/00:14:42. Disponible en: https://doi.org/10.2166/wh.2021.011. Disponible en: 10.2166/wh.2021.011. Jinatham, Vasana; Maxamhud, Sadiya; Popluechai, Siam; Tsaousis, Anastasios D.; Gentekaki, Eleni (2021) Blastocystis One Health Approach in a Rural Community of Northern Thailand: Prevalence, Subtypes and Novel Transmission Routes. En: Frontiers in Microbiology. Vol. 12; 1664-302X; Consultado en: 2022/06/30/22:42:29. Disponible en: https://www.frontiersin.org/article/10.3389/fmicb.2021.746340. Clark, C. Graham; van der Giezen, Mark; Alfellani, Mohammed A.; Stensvold, C. Rune; Rollinson, D. (2013) Chapter One. En: Advances in Parasitology. Vol. 82; pp. 1 - 32; Academic Press; Consultado en: 2022/07/07/01:35:11. Disponible en: https://www.sciencedirect.com/science/article/pii/B9780124077065000010. Baek, Sohyeong; Maloney, Jenny G.; Molokin, Aleksey; George, Nadja S.; Cortés Vecino, Jesús A.; Santin, Monica (2022) Diversity of Blastocystis Subtypes in Horses in Colombia and Identification of Two New Subtypes. En: Microorganisms. Vol. 10; No. 9; pp. 1693 2076-2607; Consultado en: 2022/10/07/16:43:23. Disponible en: https://www.mdpi.com/2076-2607/10/9/1693. Disponible en: 10.3390/microorganisms10091693. Wylezich, Claudia; Belka, Ariane; Hanke, Dennis; Beer, Martin; Blome, Sandra; Höper, Dirk (2019) Metagenomics for broad and improved parasite detection: a proof-of-concept study using swine faecal samples. En: International Journal for Parasitology. Vol. 49; No. 10; pp. 769 - 777; 0020-7519; Consultado en: 2023/03/20/04:14:20. Disponible en: https://www.sciencedirect.com/science/article/pii/S0020751919301869. Disponible en: 10.1016/j.ijpara.2019.04.007. AbuOdeh, Raed; Ezzedine, Sinda; Madkour, Mohamed; Stensvold, Christen Rune; Samie, Amidou; Nasrallah, Gheyath; AlAbsi, Enas; ElBakri, Ali (2019) Molecular Subtyping of Blastocystis from Diverse Animals in the United Arab Emirates. En: Protist. Vol. 170; No. 5; pp. 125679 1434-4610; Consultado en: 2023/03/20/07:05:51. Disponible en: https://www.sciencedirect.com/science/article/pii/S1434461019300094. Disponible en: 10.1016/j.protis.2019.125679. Onder, Zuhal; Yildirim, Alparslan; Pekmezci, Didem; Duzlu, Onder; Pekmezci, Gokmen Zafer; Ciloglu, Arif; Simsek, Emrah; Kokcu, Nesrin Delibasi; Yetismis, Gamze; Ercan, Nuri; Inci, Abdullah (2021) Molecular identification and subtype distribution of Blastocystis sp. in farm and pet animals in Turkey. En: Acta Tropica. Vol. 220; pp. 105939 0001-706X; Consultado en: 2023/03/20/07:10:55. Disponible en: https://www.sciencedirect.com/science/article/pii/S0001706X21001182. Disponible en: 10.1016/j.actatropica.2021.105939. Udonsom, Ruenruetai; Prasertbun, Rapeepan; Mahittikorn, Aongart; Mori, Hirotake; Changbunjong, Tanasak; Komalamisra, Chalit; Pintong, Ai-rada; Sukthana, Yaowalark; Popruk, Supaluk (2018) Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand. En: Infection, Genetics and Evolution. Vol. 65; pp. 107 - 111; 1567-1348; Consultado en: 2023/03/20/08:25:06. Disponible en: https://www.sciencedirect.com/science/article/pii/S1567134818304805. Disponible en: 10.1016/j.meegid.2018.07.007. Jiménez, Paula A.; Munoz, Marina; Ramirez, Juan David; An update on the distribution of Blastocystis subtypes in the Americas. Consultado en: 2023/03/20/10:54:44. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816782/. Salehi, Roya; Rostami, Ali; Mirjalali, Hamed; Stensvold, Christen Rune; Haghighi, Ali (2022) Genetic characterization of Blastocystis from poultry, livestock animals and humans in the southwest region of Iran—Zoonotic implications. En: Transboundary and Emerging Diseases. Vol. 69; No. 3; pp. 1178 - 1185; Maloney, Jenny G.; Molokin, Aleksey; Seguí, Raimundo; Maravilla, Pablo; Martínez-Hernández, Fernando; Villalobos, Guiehdani; Tsaousis, Anastasios D.; Gentekaki, Eleni; Muñoz-Antolí, Carla; Klisiowicz, Debora R.; Oishi, Camila Y.; Toledo, Rafael; Esteban, J. Guillermo; Köster, Pamela C.; de Lucio, Aida; Dashti, Alejandro; Bailo, Begoña; Calero-Bernal, Rafael; González-Barrio, David; Carmena, David; Santín, Mónica (2023) Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38. En: Microorganisms. Vol. 11; No. 1; pp. 46 2076-2607; Consultado en: 2023/08/10/08:12:44. Disponible en: https://www.mdpi.com/2076-2607/11/1/46. Disponible en: 10.3390/microorganisms11010046. Rudzińska, Monika; Kowalewska, Beata; Kurpas, Monika; Szostakowska, Beata (2022) Rare Occurrence of Blastocystis in Pet Animals and Their Owners in the Pomeranian Voivodeship in Poland in the Light of Literature Data. En: Journal of Clinical Medicine. Vol. 11; No. 11; pp. 2975 2077-0383; Consultado en: 2023/08/10/09:09:54. Disponible en: https://www.mdpi.com/2077-0383/11/11/2975. Disponible en: 10.3390/jcm11112975. Rauff-Adedotun, Adedolapo Aminat; Mohd Zain, Siti Nursheena; Farah Haziqah, Meor Termizi (2020) Current status of Blastocystis sp. in animals from Southeast Asia: a review. En: Parasitology Research. Vol. 119; No. 11; pp. 3559 - 3570; 1432-1955; Consultado en: 2023/08/10/13:16:50. Disponible en: https://doi.org/10.1007/s00436-020-06828-8. Disponible en: 10.1007/s00436-020-06828-8. Naguib, Doaa; Gantois, Nausicaa; Desramaut, Jeremy; Arafat, Nagah; Even, Gaël; Certad, Gabriela; Chabé, Magali; Viscogliosi, Eric (2022) Prevalence, Subtype Distribution and Zoonotic Significance of Blastocystis sp. Isolates from Poultry, Cattle and Pets in Northern Egypt. En: Microorganisms. Vol. 10; No. 11; pp. 2259 2076-2607; Consultado en: 2023/08/10/13:26:41. Disponible en: https://www.mdpi.com/2076-2607/10/11/2259. Disponible en: 10.3390/microorganisms10112259. Russini, Valeria; Di Filippo, Margherita Montalbano; Fanelli, Rita; Polidori, Miriam; Berrilli, Federica; Di Cave, David; Novelletto, Andrea; Calderini, Pietro (2020) Characterization of prevalence and genetic subtypes of Blastocystis sp. in wild and domestic Suidae of central Italy aided by amplicon NGS. En: Veterinary Parasitology: Regional Studies and Reports. Vol. 22; pp. 100472 2405-9390; Consultado en: 2023/08/10/13:52:18. Disponible en: https://www.sciencedirect.com/science/article/pii/S2405939020302537. Disponible en: 10.1016/j.vprsr.2020.100472. Asghari, Ali; Sadrebazzaz, Alireza; Shamsi, Laya; Shams, Morteza (2021) Global prevalence, subtypes distribution, zoonotic potential, and associated risk factors of Blastocystis sp. in domestic pigs (Sus domesticus) and wild boars (Sus scrofa): A systematic review and meta-analysis. En: Microbial Pathogenesis. Vol. 160; pp. 105183 0882-4010; Consultado en: 2023/08/10/13:52:55. Disponible en: https://www.sciencedirect.com/science/article/pii/S0882401021004575. Disponible en: 10.1016/j.micpath.2021.105183. Chen, Hongshuang; Hao, Yaru; Liu, Yuqing; Xu, Mingqi; Zhang, Weizhe; Li, He; Yang, Fengkun (2023) The frequency and subtype distribution of Blastocystis sp. in humans and domestic animals in households in Heilongjiang Province, China. En: Acta Tropica. Vol. 240; pp. 106844 0001-706X; Consultado en: 2023/08/10/14:24:38. Disponible en: https://www.sciencedirect.com/science/article/pii/S0001706X23000311. Disponible en: 10.1016/j.actatropica.2023.106844. Li, Junqiang; Karim, Md. Robiul; Li, Dongfang; Rahaman Sumon, S. M. Mostafizur; Siddiki, S. H. M. Faruk; Rume, Farzana Islam; Sun, Ruiqin; Jia, Yaquan; Zhang, Longxian (2019) Molecular characterization of Blastocystis sp. in captive wildlife in Bangladesh National Zoo: Non-human primates with high prevalence and zoonotic significance. En: International Journal for Parasitology: Parasites and Wildlife. Vol. 10; pp. 314 - 320; 2213-2244; Consultado en: 2023/08/10/14:27:01. Disponible en: https://www.sciencedirect.com/science/article/pii/S2213224419302378. Disponible en: 10.1016/j.ijppaw.2019.11.003. Rauff-Adedotun, Adedolapo Aminat; Lee, Ii Li; Abd Talib, Nabilah; Shaari, Nurshafarina; Yahaya, Zary Shariman; Meor Termizi, Farah Haziqah (2023) Prevalence, potential risk factors and genetic diversity of Blastocystis in ruminant livestock animals from Penang, Malaysia. En: Parasitology Research. 1432-1955; Consultado en: 2023/08/10/14:27:17. Disponible en: https://doi.org/10.1007/s00436-023-07920-5. Disponible en: 10.1007/s00436-023-07920-5. Poulsen, Casper S.; Efunshile, Akinwale M.; Nelson, Jenna A.; Stensvold, Christen R. (2016) Epidemiological Aspects of Blastocystis Colonization in Children in Ilero, Nigeria. En: The American Journal of Tropical Medicine and Hygiene. Vol. 95; No. 1; pp. 175 - 179; 0002-9637; Consultado en: 2023/08/10/14:36:02. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944538/. Disponible en: 10.4269/ajtmh.16-0074. Munsaka, Gilbert; Hayashida, Kyoko; Mubemba, Benjamin; Simulundu, Edgar; Mulunda, Namwiinga; Pule, Ruth; Sianongo, Sandie; Makuluni, Marina; Muleya, Walter; Changula, Katendi; Chitanga, Simbarashe; Mutengo, Mable (2022) Molecular subtyping of Blastocystis sp. detected in patients at a large tertiary referral hospital in Lusaka, Zambia. En: Frontiers in Parasitology. Vol. 1; 2813-2424; Consultado en: 2023/08/10/14:36:13. Disponible en: https://www.frontiersin.org/articles/10.3389/fpara.2022.1033485. Ahmed, Shahira A.; El-Mahallawy, Heba S.; Mohamed, Samar Farag; Angelici, Maria Cristina; Hasapis, Kyriacos; Saber, Taisir; Karanis, Panagiotis (2022) Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt. En: Scientific Reports. Vol. 12; No. 1; pp. 19084 2045-2322; Consultado en: 2023/08/10/14:40:58. Disponible en: https://www.nature.com/articles/s41598-022-23360-0. Disponible en: 10.1038/s41598-022-23360-0. Deng, Lei; Wojciech, Lukasz; Png, Chin Wen; Kioh, Dorinda Yan Qin; Gu, Yuxiang; Aung, Thet Tun; Malleret, Benoit; Chan, Eric Chun Yong; Peng, Guangneng; Zhang, Yongliang; Gascoigne, Nicholas Robert John; Tan, Kevin Shyong Wei (2023) Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. En: Theranostics. Vol. 13; No. 3; pp. 1165 - 1179; 1838-7640; Disponible en: 10.7150/thno.81583. Danišová, Oľga; Valenčáková, Alexandra (2021) First detection of Blastocystis sp. in pigs in Slovakia and in Europe. En: Parasitology International. Vol. 81; pp. 102235 1383-5769; Consultado en: 2023/08/10/14:59:06. Disponible en: https://www.sciencedirect.com/science/article/pii/S1383576920301859. Disponible en: 10.1016/j.parint.2020.102235. Pawestri, A. R.; Padungsombat, J.; Deesitthivech, O.; Nakayama, E.; Shioda, T.; Moonsom, S. (2022) Blastocystis spp. Subtype Distribution from Human and Animals at the Thai-Myanmar Border: The Public Health Implication. En: International Journal of Infectious Diseases. Abstracts from the Eighth International Meeting on Emerging Diseases and Surveillance, IMED 2021; Vol. 116; pp. S80 1201-9712; Consultado en: 2023/09/06/09:32:26. Disponible en: https://www.sciencedirect.com/science/article/pii/S1201971221010821. Disponible en: 10.1016/j.ijid.2021.12.190. Santin, Monica; Figueiredo, Ana; Molokin, Aleksey; George, Nadja S; Köster, Pamela C.; Dashti, Alejandro; González-Barrio, David; Carmena, David; Maloney, Jenny G.; Division of Blastocystis ST10 into three new subtypes: ST42-ST44. En: Journal of Eukaryotic Microbiology. Vol. n/a; No. n/a; pp. e12998 1550-7408; Consultado en: 2023/09/11/06:25:04. Disponible en: https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12998. Disponible en: 10.1111/jeu.12998. Stensvold, Christen Rune; Berg, Rebecca P. K. D.; Maloney, Jenny G.; Molokin, Aleksey; Santin, Monica (2023) Molecular characterization of Blastocystis and Entamoeba of muskoxen and sheep in Greenland. En: International Journal for Parasitology. Vol. 53; No. 11-12; pp. 673 - 685; 1879-0135; Disponible en: 10.1016/j.ijpara.2023.05.005. Hernández-Castro, Carolina; Maloney, Jenny G.; Agudelo-López, Sonia P.; Toro-Londoño, Miguel A.; Botero-Garcés, Jorge H.; Orozco, María C.; Quintero-Quinchia, Yulieth C.; Correa-Cote, Juan C.; Múnera-Duque, Alejandro; Ricaurte-Ciro, Juan C.; Londoño-Álvarez, Luis I.; Escobar, René M.; Köster, Pamela C.; Sánchez, Sergio; Carmena, David; Santín, Mónica (2023) Identification and validation of novel Blastocystis subtype ST41 in a Colombian patient undergoing colorectal cancer screening. En: The Journal of Eukaryotic Microbiology. Vol. 70; No. 5; pp. e12978 1550-7408; Disponible en: 10.1111/jeu.12978. Rondón, Silvia; Cavallero, Serena; Link, Andrés; González, Camila; D’Amelio, Stefano (2023) Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia. En: Pathogens. Vol. 12; No. 4; pp. 569 2076-0817; Consultado en: 2023/09/11/06:28:16. Disponible en: https://www.mdpi.com/2076-0817/12/4/569. Disponible en: 10.3390/pathogens12040569. Cruz-Saavedra, Lissa; Arévalo, Vivian Alejandra; Garcia-Corredor, Diego; Jiménez, Paula Andrea; Vega, Laura; Pulido-Medellín, Martín; Ortiz-Pineda, Melissa; Ramírez, Juan David (2023) Molecular detection and characterization of Giardia spp., Cryptosporidium spp., and Blastocystis in captive wild animals rescued from central Colombia. En: International Journal for Parasitology: Parasites and Wildlife. Vol. 22; pp. 1 - 5; 2213-2244; Disponible en: https://www.sciencedirect.com/science/article/pii/S2213224423000573. Disponible en: https://doi.org/10.1016/j.ijppaw.2023.07.005. Hernández, Paula C.; Maloney, Jenny G.; Molokin, Aleksey; George, Nadja S.; Morales, Liliana; Chaparro-Olaya, Jacqueline; Santin, Monica (2023) Exploring Blastocystis genetic diversity in rural schoolchildren from Colombia using next-generation amplicon sequencing reveals significant associations between contact with animals and infection risk. En: Parasitology Research. Vol. 122; No. 7; pp. 1451 - 1462; 1432-1955; Consultado en: 2023/10/22/10:09:27. Disponible en: https://doi.org/10.1007/s00436-023-07841-3. Disponible en: 10.1007/s00436-023-07841-3. Mokhtar, Amira; Youssef, Ahmed (2018) Subtype analysis of Blastocystis spp. isolated from domestic mammals and poultry and its relation to transmission to their in-contact humans in Ismailia governorate, Egypt. En: Parasitologists United Journal. Vol. 11; No. 2; pp. 90 - 98; Paulos, Silvia; Köster, Pamela C.; de Lucio, Aida; Hernández-de-Mingo, Marta; Cardona, Guillermo A.; Fernández-Crespo, Juan C.; Stensvold, Christen R.; Carmena, David (2018) Occurrence and subtype distribution of Blastocystis sp. in humans, dogs and cats sharing household in northern Spain and assessment of zoonotic transmission risk. En: Zoonoses and Public Health. Vol. 65; No. 8; pp. 993 - 1002; 1863-2378; Disponible en: 10.1111/zph.12522. Potes-Morales, Caterine; Crespo-Ortiz, Maria del Pilar (2023) Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America. En: PLOS ONE. Vol. 18; No. 5; pp. e0283824 1932-6203; Consultado en: 2023/11/05/21:45:49. Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283824. Disponible en: 10.1371/journal.pone.0283824. Mahdavi, Farzad; Asghari, Ali; Shahabi, Saeed; Shamsi, Laya; Soltani-Jazi, Farzaneh; Sadrebazzaz, Alireza; Shams, Morteza (2022) Distribution, genetic diversity, and zoonotic significance of Blastocystis subtypes in pet dogs. En: Comparative Immunology, Microbiology and Infectious Diseases. Vol. 88; pp. 101848 0147-9571; Consultado en: 2023/11/05/21:48:35. Disponible en: https://www.sciencedirect.com/science/article/pii/S0147957122001059. Disponible en: 10.1016/j.cimid.2022.101848. Huson, Daniel H.; Bryant, David (2006) Application of phylogenetic networks in evolutionary studies. En: Molecular Biology and Evolution. Vol. 23; No. 2; pp. 254 - 267; 0737-4038; Disponible en: 10.1093/molbev/msj030. Xu, Qingbiao; Qiao, Qinqin; Gao, Ya; Hou, Jinxiu; Hu, Mingyang; Du, Yufeng; Zhao, Ke; Li, Xiang (2021) Gut Microbiota and Their Role in Health and Metabolic Disease of Dairy Cow. En: Frontiers in Nutrition. Vol. 8; 2296-861X; Consultado en: 2023/11/06/06:32:43. Disponible en: https://www.frontiersin.org/articles/10.3389/fnut.2021.701511. Rudzińska, Monika; Sikorska, Katarzyna (2023) Epidemiology of Blastocystis Infection: A Review of Data from Poland in Relation to Other Reports. En: Pathogens. Vol. 12; No. 8; pp. 1050 2076-0817; Consultado en: 2023/11/06/12:29:36. Disponible en: https://www.mdpi.com/2076-0817/12/8/1050. Disponible en: 10.3390/pathogens12081050. Delahaye, Clara; Nicolas, Jacques (2021) Sequencing DNA with nanopores: Troubles and biases. En: PLOS ONE. Vol. 16; No. 10; pp. e0257521 1932-6203; Consultado en: 2023/11/24/07:28:32. Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257521. Disponible en: 10.1371/journal.pone.0257521. Sahlin, Kristoffer; Medvedev, Paul (2021) Error correction enables use of Oxford Nanopore technology for reference-free transcriptome analysis. En: Nature Communications. Vol. 12; No. 1; pp. 2 2041-1723; Consultado en: 2023/11/24/13:21:47. Disponible en: https://www.nature.com/articles/s41467-020-20340-8. Disponible en: 10.1038/s41467-020-20340-8.
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spelling	Ramírez González, Juan David1011716118600Muñoz Díaz, Claudia Marinaed1d1be3-30ee-46d3-95f3-0bde7b16b402-1Grupo de Investigaciones Microbiológicas UR (GIMUR)Jiménez Jiménez, Paula AndreaMuñoz Díaz, Claudia MarinaCruz-Saavedra, LissaCamargo, AnnyRamírez González, Juan DavidMagíster en Ciencias NaturalesMaestríaFull time5b594054-8e2e-4437-b659-b652f6248ab6-1d98eba3e-89b0-474a-b32e-fa70209f94d5-13a39d4a1-8a8d-431d-951a-63c54f374456-13b7490f7-a153-437b-aba0-f5692e5bde64-110117161186002024-05-08T20:35:38Z2024-05-08T20:35:38Z2023-11-29Blastocystis es un microeucariota intestinal que ha suscitado atención debido a su amplia distribución en animales y seres humanos. El riesgo de circulación zoonótica surge principalmente del contacto estrecho con animales infectados. Por lo tanto, el siguiente estudio tuvo como objetivo evaluar la diversidad y frecuencia de los subtipos de Blastocystis en muestras humanas y animales colombianas utilizando la secuenciación completa del gen 18S rRNA. Para este propósito, 341 muestras fecales humanas y 277 muestras fecales animales (de bovinos, ovinos, caprinos, cerdos, gatos y perros), fueron recolectadas de diferentes regiones colombianas y analizadas usando detección basada en PCR y secuenciación de próxima generación (NGS) del gen 18S SSU rRNA de longitud completa. Entre las 618 muestras de ambos huéspedes, humanos y animales, los resultados revelaron una frecuencia generalizada de Blastocystis, con un 48,09% (n=164) en humanos y un 31,4% (n=87) de detección en animales. Perros, gatos, ovejas, cerdos y animales salvajes dieron positivo, en consonancia con los patrones de prevalencia mundial. Además, se secuenciaron 29 muestras humanas y 23 muestras animales mediante tecnología ONT, a partir de las cuales se generaron 11 secuencias únicas de lectura larga que se agruparon con sus secuencias de referencia comparadas. La distribución de subtipos varió dentro de los hospedadores, detectándose ST1 y ST3 tanto en muestras humanas como animales. Los subtipos ST5, ST10, ST14, ST15, ST21, ST24, ST25 y ST26 se limitaron a hospedadores animales, algunos de los cuales se considera que tienen potencial zoonótico. Por otra parte, el ST2 se encontró exclusivamente en muestras humanas de la región de Bolívar. Se produjeron infecciones mixtas tanto en animales como en humanos, 60,86% y 27,58% respectivamente. Además, hasta donde sabemos, este es el primer estudio en Colombia que identifica ST15 en cerdos y ST25 en ovejas. Los subtipos (STs) identificados en este estudio indican que ciertos animales pueden servir como reservorios con potencial de transmisión zoonótica. La identificación de subtipos zoonóticos pone de relieve el uso de la secuenciación de nueva generación, ya que la profundidad y la resolución de las secuencias aumentan, lo que permite comprender mejor los ST de importancia médica y veterinaria. También revela la coexistencia de diversos subtipos entre hospedadores. Es esencial seguir investigando para comprender la dinámica de transmisión, las implicaciones sanitarias y las estrategias de detección de Blastocystis en animales y humanos, sobre todo por el papel de los animales como reservorios y su estrecha interacción con los humanos.Blastocystis is an intestinal microeukaryote that has raised attention due to its wide distribution in animals and humans. The risk of zoonotic circulation primarily arises from close contact with infected animals. Therefore, the following study aimed to evaluate the diversity and frequency of Blastocystis subtypes in Colombian human and animal samples using complete sequencing of the 18S rRNA gene. For this purpose, 341 human stool samples and 277 animal fecal samples (from cattle, sheep, goat, pigs, cats, and dogs), were collected from different Colombian regions and analyzed using PCR-based detection and full-length 18S SSU rRNA gene Next-Generation Sequencing (NGS). Among the 618 samples from both hosts, humans and animals, the results revealed widespread Blastocystis frequency, with 48.09% (n = 164) in humans and 31.4% (n = 87) detection in animals. Dogs, cats, sheep, pigs, and wild animals tested positive, aligning with global prevalence patterns. Also, 29 human samples and 23 animal samples were sequenced using ONT technology from which 11 long-read unique sequences were generated and cluster with their compared reference sequences. The subtype distribution varied within hosts, detecting ST1 and ST3 in both human and animal samples. Subtypes ST5, ST10, ST14, ST15, ST21, ST24, ST25 and ST26 were limited to animals hosts, some of which are considered to have zoonotic potential. On the other hand, ST2 was found exclusively in human samples from Bolivar region. Mixed infections occurred in both animal and humans, 60.86% and 27.58% respectively. Moreover, to our knowledge, this is the first study in Colombia identifying ST15 in pigs and ST25 in sheep. The subtypes (STs) identified in this study indicate that certain animals may serve as reservoirs with the potential for zoonotic transmission. The identification of zoonotic subtypes highlights the use of Next Generation Sequencing as the depth and resolution of the sequences increases providing insights into STs of medical and veterinarian significance. It also reveals the coexistence of diverse subtypes among hosts. Further research is essential for understanding transmission dynamics, health implications, and detection strategies for Blastocystis occurrence in animals and humans, mainly associated to the role of animals as reservoirs and their close interaction with humans.49 ppapplication/pdfhttps://doi.org/10.1016/j.actatropica.2023.107090https://repository.urosario.edu.co/handle/10336/42536engUniversidad del RosarioFacultad de Ciencias NaturalesMaestría en Ciencias NaturalesAttribution-NonCommercial-NoDerivatives 4.0 InternationalAbierto (Texto Completo)Universidad del Rosariohttp://creativecommons.org/licenses/by-nc-nd/4.0/http://purl.org/coar/access_right/c_abf2Ajjampur, Sitara SR; Tan, Kevin SW (2016) Pathogenic mechanisms in Blastocystis spp.—Interpreting results from in vitro and in vivo studies. En: Parasitology international. Vol. 65; No. 6; pp. 772 - 779; 1383-5769;Alfellani, Mohammed A; Stensvold, C Rune; Vidal-Lapiedra, Amparo; Onuoha, Emeh Sunday Uche; Fagbenro-Beyioku, Adetayo F; Clark, C Graham (2013) Variable geographic distribution of Blastocystis subtypes and its potential implications. En: Acta tropica. Vol. 126; No. 1; pp. 11 - 18; 0001-706X;Alfellani, Mohammed A; Taner-Mulla, Derya; Jacob, Alison S; Imeede, Christine Atim; Yoshikawa, Hisao; Stensvold, C Rune; Clark, C Graham (2013) Genetic diversity of Blastocystis in livestock and zoo animals. En: Protist. Vol. 164; No. 4; pp. 497 - 509; 1434-4610;Barbosa, Carolina Valença; Batista, Rosemary de Jesus; Igreja, Ricardo Pereira; Levy, Claudia Masini d’Avila; Werneck de Macedo, Heloisa; Santos, Helena Lúcia Carneiro (2017) Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil. En: Parasites & Vectors, Vol 10, Iss 1, Pp 1-9 (2017). No. 1; pp. 1 1756-3305; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.97501ef7c2c5465092343ee6868b417e〈=es&site=eds-live&scope=site. Disponible en: 10.1186/s13071-017-2458-0.Casero, Rodolfo Daniel; Mongi, Florencia; Sánchez, Angie; Ramírez, Juan David (2015) Blastocystis and urticaria: Examination of subtypes and morphotypes in an unusual clinical manifestation. En: Acta Tropica. Vol. 148; pp. 156 - 161; 0001-706X; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S0001706X15001382〈=es&site=eds-live&scope=site. Disponible en: 10.1016/j.actatropica.2015.05.004.Cian, Amandine; El Safadi, Dima; Osman, Marwan; Moriniere, Romain; Gantois, Nausicaa; Benamrouz-Vanneste, Sadia; Delgado-Viscogliosi, Pilar; Guyot, Karine; Li, Luen-Luen; Monchy, Sébastien (2017) Molecular epidemiology of Blastocystis sp. in various animal groups from two French zoos and evaluation of potential zoonotic risk. En: PloS one. Vol. 12; No. 1; pp. e0169659 1932-6203;Deng, Lei; Wojciech, Lukasz; Gascoigne, Nicholas RJ; Peng, Guangneng; Tan, Kevin SW (2021) New insights into the interactions between Blastocystis, the gut microbiota, and host immunity. En: PLoS Pathogens. Vol. 17; No. 2; pp. e1009253 1553-7366;Gabrielli, S.; Furzi, F.; Fontanelli Sulekova, L.; Taliani, G.; Mattiucci, S. (2020) Occurrence of Blastocystis-subtypes in patients from Italy revealed association of ST3 with a healthy gut microbiota. En: Parasite Epidemiol Control. Vol. 9; pp. e00134 2405-6731; Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/32258445. Disponible en: 10.1016/j.parepi.2020.e00134.Higuera, Adriana; Herrera, Giovanny; Jimenez, Paula; García-Corredor, Diego; Pulido-Medellín, Martin; Bulla-Castañeda, Diana M; Pinilla, Juan Carlos; Moreno-Pérez, Darwin A; Maloney, Jenny G; Santín, Mónica (2021) Identification of multiple Blastocystis subtypes in domestic animals from Colombia using amplicon-based next generation sequencing. En: Frontiers in veterinary science. pp. 932 2297-1769;Higuera, Adriana; Muñoz, Marina; López, Myriam Consuelo; Reyes, Patricia; Urbano, Plutarco; Villalobos, Oswaldo; Ramírez, Juan David (2020) Succinate dehydrogenase gene as a marker for studying Blastocystis genetic diversity. En: Heliyon. Vol. 6; No. 11; pp. e05387 2405-8440;Hublin, Josephine SY; Maloney, Jenny G; Santin, Monica (2020) Blastocystis in domesticated and wild mammals and birds. En: Research in Veterinary Science. 0034-5288;Iebba, V.; Santangelo, F.; Totino, V.; Pantanella, F.; Monsia, A.; Di Cristanziano, V.; Di Cave, D.; Schippa, S.; Berrilli, F.; D'Alfonso, R. (2016) Gut microbiota related to Giardia duodenalis, Entamoeba spp. and Blastocystis hominis infections in humans from Côte d'Ivoire. En: J Infect Dev Ctries. Vol. 10; No. 9; pp. 1035 - 1041; 1972-2680; Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/27694739. Disponible en: 10.3855/jidc.8179.Jiménez, Paula Andrea; Jaimes, Jesús Eduardo; Ramírez, Juan David (2019) A summary of Blastocystis subtypes in North and South America. En: Parasites & vectors. Vol. 12; No. 1; pp. 1 - 9; 1756-3305;Lee, Li; Chye, Tan; Karmacharya, Biraj; Govind, Suresh (2012) Blastocystis sp.: waterborne zoonotic organism, a possibility?. En: Parasites & Vectors, Vol 5, Iss 1, p 130 (2012). No. 1; pp. 130 1756-3305; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.f031f489a68c49b5a34b86d9c081a859〈=es&site=eds-live&scope=site. Disponible en: 10.1186/1756-3305-5-130.Li, Lan-Hua; Zhou, Xiao-Nong; Du, Zun-Wei; Wang, Xue-Zhong; Wang, Li-Bo; Jiang, Jin-Yong; Yoshikawa, Hisao; Steinmann, Peter; Utzinger, Jürg; Wu, Zhiliang; Chen, Jia-Xu; Chen, Shao-Hong; Zhang, Ling (2007) Molecular epidemiology of human Blastocystis in a village in Yunnan province, China. En: Parasitology International. Vol. 56; No. 4; pp. 281 - 286; 1383-5769; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=cmedm&AN=17627869〈=es&site=eds-live&scope=site.Londoño-Franco, Ángela Liliana; Loaiza-Herrera, Juliana; Lora-Suárez, Fabiana María; Gómez-Marín, Jorge Enrique (2014) Frecuencia y fuentes de Blastocystis sp . en niños de 0 a 5 años de edad atendidos en hogares infantiles públicos de la zona urbana de Calarcá, Colombia / Blastocystis sp . frequency and sources among children from 0 to 5 years of age attending public day care centers in Calarcá, Colombia. En: Biomédica. No. 2; pp. 218 0120-4157; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edssci&AN=edssci.S0120.41572014000200008〈=es&site=eds-live&scope=site. Disponible en: 10.7705/biomedica.v34i2.2124.Maloney, Jenny G; da Cunha, Maria JR; Molokin, Aleksey; Cury, Márcia C; Santin, Monica (2021) Next-generation sequencing reveals wide genetic diversity of Blastocystis subtypes in chickens including potentially zoonotic subtypes. En: Parasitology Research. pp. 1 - 13; 1432-1955;Maloney, Jenny G; Jang, Yunah; Molokin, Aleksey; George, Nadja S; Santin, Monica (2021) Wide Genetic Diversity of Blastocystis in White-Tailed Deer (Odocoileus virginianus) from Maryland, USA. En: Microorganisms. Vol. 9; No. 6; pp. 1343 Maloney, Jenny G; Molokin, Aleksey; da Cunha, Maria Júlia Rodrigues; Cury, Márcia Cristina; Santin, Monica (2020) Blastocystis subtype distribution in domestic and captive wild bird species from Brazil using next generation amplicon sequencing. En: Parasite epidemiology and control. Vol. 9; pp. e00138 2405-6731;Maloney, Jenny G; Molokin, Aleksey; Santin, Monica (2019) Next generation amplicon sequencing improves detection of Blastocystis mixed subtype infections. En: Infection, Genetics and Evolution. Vol. 73; pp. 119 - 125; 1567-1348;Maloney, Jenny G; Molokin, Aleksey; Santin, Monica (2020) Use of Oxford Nanopore MinION to generate full-length sequences of the Blastocystis small subunit (SSU) rRNA gene. En: Parasites & vectors. Vol. 13; No. 1; pp. 1 - 8; 1756-3305;Maloney, Jenny G; Santin, Monica (2021) Mind the gap: New full-length sequences of Blastocystis subtypes generated via Oxford Nanopore Minion sequencing allow for comparisons between full-length and partial sequences of the small subunit of the ribosomal RNA gene. En: Microorganisms. Vol. 9; No. 5; pp. 997 Maloney, Jenny G.; Lombard, Jason E.; Urie, Natalie J.; Shivley, Chelsey B.; Santin, Monica (2018) Zoonotic and genetically diverse subtypes of Blastocystis in US pre-weaned dairy heifer calves. En: Parasitology Research. Vol. 2; No. 118; pp. 575 - 582; 1432-1955; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=cmedm&AN=30483890〈=es&site=eds-live&scope=site. Disponible en: 10.1007/s00436-018-6149-3.Ministerio de Salud y Protección Social (2015) Encuesta Nacional de Parasitismo Intestinal en Población Escolar, Colombia 2012. : Facultad Nacional Salud Pública, Universidad de Antioquia; Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/PP/ET/encuesta-nacional-de-parasitismo-2012-2014.pdf.Peña, S.; Carrasco, G.; Rojas, P.; Castillo, D.; Ozaki, L. S.; Mercado, R. (2020) Determination of subtypes of Blastocystis sp. in Chilean patients with and without inflammatory bowel syndrome, A preliminary report. En: Parasite Epidemiol Control. Vol. 8; pp. e00125 2405-6731; Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/31890923. Disponible en: 10.1016/j.parepi.2019.e00125.Peña, S.; Carrasco, G.; Rojas, P.; Gotteland, M.; Castillo, D.; Ozaki, L. S.; Mercado, R. (2019) Diferent Subtypes of Blastocystis hominis (ST1, ST2 and ST4) are present in Chilean patients with Inflamatory Bowel Disease. Qi, Meng; Wei, Zilin; Zhang, Ying; Zhang, Qiyuan; Li, Juanfeng; Zhang, Longxian; Wang, Rongjun (2020) Genetic diversity of Blastocystis in kindergarten children in southern Xinjiang, China. En: Parasites & Vectors. Vol. 13; No. 1; pp. 1 - 6; 1756-3305;Ramirez, Juan David; Sanchez, Angie; Hernandez, Carolina; Florez, Carolina; Consuelo Bernal, Maria; Cesar Giraldo, Julio; Reyes, Patricia; Consuelo Lopez, Myriam; Garcia, Lineth; Cooper, Philip J.; Vicuna, Yosselin; Mongi, Florencia; Casero, Rodolfo D. (2016) Geographic distribution of human Blastocystis subtypes in South America. Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edswsc&AN=000375652800006〈=es&site=eds-live&scope=site.Ramírez, Juan David; Flórez, Carolina; Olivera, Mario; Bernal, María Consuelo; Giraldo, Julio Cesar (2017) Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia. En: PLoS ONE. Vol. 12; No. 2; pp. 1 - 13; 19326203; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=121368795〈=es&site=eds-live&scope=site. Disponible en: 10.1371/journal.pone.0172586.Roberts, Tamalee; Stark, Damien; Harkness, Jock; Ellis, John (2013) Subtype distribution of Blastocystis isolates from a variety of animals from New South Wales, Australia. En: Veterinary Parasitology. Vol. 196; No. 1-2; pp. 85 - 89; 0304-4017;Ruaux, G., Craig; Stang, Bernadette (2014) Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest. En: PLoS ONE, Vol 9, Iss 9, p e107496 (2014). No. 9; pp. e107496 1932-6203; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edsdoj&AN=edsdoj.921334fb092043a6a8c7c8f27e64d73a〈=es&site=eds-live&scope=site. Disponible en: 10.1371/journal.pone.0107496.Stensvold, C. Rune; Alfellani, Mohammed; Clark, C. Graham (2012) Levels of genetic diversity vary dramatically between Blastocystis subtypes. En: Infection, Genetics and Evolution. Vol. 12; pp. 263 - 273; 1567-1348; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S156713481100387X〈=es&site=eds-live&scope=site. Disponible en: 10.1016/j.meegid.2011.11.002.Stensvold, C. Rune; Clark, C. Graham (2016) Current status of Blastocystis: A personal view. En: Parasitology International. Vol. 65; No. Part B; pp. 763 - 771; 1383-5769; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S1383576916301544〈=es&site=eds-live&scope=site. Disponible en: 10.1016/j.parint.2016.05.015.Stensvold, Christen Rune (2013) Blastocystis: Genetic diversity and molecular methods for diagnosis and epidemiology. En: Tropical Parasitology. Vol. 3; No. 1; pp. 26 - 34; 22295070; Disponible en: http://ez.urosario.edu.co/login?url=http://search.ebscohost.com/login.aspx?direct=true&db=a9h&AN=89188102〈=es&site=eds-live&scope=site. Disponible en: 10.4103/2229-5070.113896.Stensvold, Christen Rune; Ahmed, Umran Nisar; Andersen, Lee O'Brien; Nielsen, Henrik Vedel (2012) Development and evaluation of a genus-specific, probe-based, internal-process-controlled real-time PCR assay for sensitive and specific detection of Blastocystis spp. En: Journal of clinical microbiology. Vol. 50; No. 6; pp. 1847 - 1851; 0095-1137;Stensvold, Christen Rune; Clark, C Graham (2020) Pre-empting Pandora’s box: Blastocystis subtypes revisited. En: Trends in parasitology. Vol. 36; No. 3; pp. 229 - 232; 1471-4922;Stensvold, Christen Rune; Tan, Kevin SW; Clark, C Graham (2020) Blastocystis. En: Trends in parasitology. Vol. 36; No. 3; pp. 315 - 316; 1471-4922;Vega, Laura; Herrera, Giovanny; Muñoz, Marina; Patarroyo, Manuel A; Maloney, Jenny G; Santín, Monica; Ramírez, Juan David (2021) Gut microbiota profiles in diarrheic patients with co-occurrence of Clostridioides difficile and Blastocystis. En: PloS one. Vol. 16; No. 3; pp. e0248185 1932-6203;Wawrzyniak, Ivan; Poirier, Philippe; Viscogliosi, Eric; Dionigia, Meloni; Texier, Catherine; Delbac, Frédéric; Alaoui, Hicham El (2013) Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis. En: Therapeutic advances in infectious disease. Vol. 1; No. 5; pp. 167 - 178; 2049-9361;Yoshikawa, Hisao; Koyama, Yukiko; Tsuchiya, Erika; Takami, Kazutoshi (2016) Blastocystis phylogeny among various isolates from humans to insects. En: Parasitology international. Vol. 65; No. 6; pp. 750 - 759; 1383-5769;Zhao, GH; Hu, XF; Liu, TL; Hu, RS; Yu, ZQ; Yang, WB; Wu, YL; Yu, SK; Song, JK (2017) Molecular characterization of Blastocystis sp. in captive wild animals in Qinling Mountains. En: Parasitology research. Vol. 116; No. 8; pp. 2327 - 2333; 1432-1955;Osorio-Pulgarin, Maria I.; Higuera, Adriana; Beltran-Álzate, Juan C.; Sánchez-Jiménez, Miryan; Ramírez, Juan David (2021) Epidemiological and Molecular Characterization of Blastocystis Infection in Children Attending Daycare Centers in Medellín, Colombia. En: Biology. Vol. 10; No. 7; pp. 669 Consultado en: 2021/10/10/22:39:03. Disponible en: https://www.mdpi.com/2079-7737/10/7/669. Disponible en: 10.3390/biology10070669.Castañeda, Sergio; Muñoz, Marina; Villamizar, Ximena; Hernández, Paula C.; Vásquez, Luis Reinel; Tito, Raúl Yhossef; Ramírez, Juan David (2020) Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia. En: Parasites & Vectors. Vol. 13; No. 1; pp. 521 1756-3305; Consultado en: 2021/10/13/20:30:17. Disponible en: https://doi.org/10.1186/s13071-020-04392-9. Disponible en: 10.1186/s13071-020-04392-9.Higuera, Adriana; Villamizar, Ximena; Herrera, Giovanny; Giraldo, Julio Cesar; Vasquez-A, Luis Reinel; Urbano, Plutarco; Villalobos, Oswaldo; Tovar, Catalina; Ramírez, Juan David (2020) Molecular detection and genotyping of intestinal protozoa from different biogeographical regions of Colombia. En: PeerJ. Vol. 8; pp. e8554 2167-8359; Consultado en: 2021/10/14/00:32:23. Disponible en: https://peerj.com/articles/8554. Disponible en: 10.7717/peerj.8554.Jain, Miten; Koren, Sergey; Miga, Karen H.; Quick, Josh; Rand, Arthur C.; Sasani, Thomas A.; Tyson, John R.; Beggs, Andrew D.; Dilthey, Alexander T.; Fiddes, Ian T.; Malla, Sunir; Marriott, Hannah; Nieto, Tom; O'Grady, Justin; Olsen, Hugh E.; Pedersen, Brent S.; Rhie, Arang; Richardson, Hollian; Quinlan, Aaron R.; Snutch, Terrance P.; Tee, Louise; Paten, Benedict; Phillippy, Adam M.; Simpson, Jared T.; Loman, Nicholas J.; Loose, Matthew (2018) Nanopore sequencing and assembly of a human genome with ultra-long reads. En: Nature Biotechnology. Vol. 36; No. 4; pp. 338 - 345; 1546-1696; Consultado en: 2021/10/20/22:10:21. Disponible en: https://www.nature.com/articles/nbt.4060. Disponible en: 10.1038/nbt.4060.Bowden, Rory; Davies, Robert W.; Heger, Andreas; Pagnamenta, Alistair T.; de Cesare, Mariateresa; Oikkonen, Laura E.; Parkes, Duncan; Freeman, Colin; Dhalla, Fatima; Patel, Smita Y.; Popitsch, Niko; Ip, Camilla L. C.; Roberts, Hannah E.; Salatino, Silvia; Lockstone, Helen; Lunter, Gerton; Taylor, Jenny C.; Buck, David; Simpson, Michael A.; Donnelly, Peter (2019) Sequencing of human genomes with nanopore technology. En: Nature Communications. Vol. 10; No. 1; pp. 1869 2041-1723; Consultado en: 2021/10/20/22:11:08. Disponible en: https://www.nature.com/articles/s41467-019-09637-5. Disponible en: 10.1038/s41467-019-09637-5.Katoh, Kazutaka; Standley, Daron M. (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. En: Molecular Biology and Evolution. Vol. 30; No. 4; pp. 772 - 780; 0737-4038; Consultado en: 2021/10/26/04:30:25. Disponible en: https://doi.org/10.1093/molbev/mst010. Disponible en: 10.1093/molbev/mst010.Minh, Bui Quang; Schmidt, Heiko A; Chernomor, Olga; Schrempf, Dominik; Woodhams, Michael D; von Haeseler, Arndt; Lanfear, Robert (2020) IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era. En: Molecular Biology and Evolution. Vol. 37; No. 5; pp. 1530 - 1534; 0737-4038; Consultado en: 2021/10/26/04:48:00. Disponible en: https://doi.org/10.1093/molbev/msaa015. Disponible en: 10.1093/molbev/msaa015.Letunic, Ivica; Bork, Peer (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. En: Nucleic Acids Research. Vol. 44; No. W1; pp. W242 - 245; 1362-4962; Disponible en: 10.1093/nar/gkw290.Cabrine-Santos, Marlene; Moura, Renata Gregório Franco; Pedrosa, André Luiz; Correia, Dalmo; Oliveira-Silva, Márcia Benedita de (2021) Molecular characterization of Blastocystis subtypes isolated in the city of Uberaba, Minas Gerais State, Brazil. En: Revista da Sociedade Brasileira de Medicina Tropical. Vol. 54; 0037-8682, 1678-9849; Consultado en: 2022/01/04/23:04:29. Disponible en: http://www.scielo.br/j/rsbmt/a/8MwpT4t5JZ6QWLsnrjrqCdr/?lang=en. Disponible en: 10.1590/0037-8682-0305-2021.Alzate, Juan F.; Toro-Londoño, Miguel; Cabarcas, Felipe; Garcia-Montoya, Gisela; Galvan-Diaz, Ana (2020) Contrasting microbiota profiles observed in children carrying either Blastocystis spp. or the commensal amoebas Entamoeba coli or Endolimax nana. En: Scientific Reports. Vol. 10; No. 1; pp. 15354 2045-2322; Consultado en: 2022/02/15/20:53:08. Disponible en: https://www.nature.com/articles/s41598-020-72286-y. Disponible en: 10.1038/s41598-020-72286-y.Yañez, Claudia Muñoz; Hernández, Alejandra Méndez; Sandoval, Alondra Martínez; Domínguez, María Aurora Maravilla; Muñiz, Soraya Amalí Zavaleta; Gómez, Janeth Oliva Guangorena (2021) Prevalence of Blastocystis and its association with Firmicutes/Bacteroidetes ratio in clinically healthy and metabolically ill subjects. En: BMC Microbiology. Vol. 21; No. 1; pp. 339 1471-2180; Consultado en: 2022/03/17/20:01:07. Disponible en: https://doi.org/10.1186/s12866-021-02402-z. Disponible en: 10.1186/s12866-021-02402-z.Nemati, Sara; Zali, Mohammad Reza; Johnson, Paul; Mirjalali, Hamed; Karanis, Panagiotis (2021) Molecular prevalence and subtype distribution of Blastocystis sp. in Asia and in Australia. En: Journal of Water and Health. Vol. 19; No. 5; pp. 687 - 704; 1477-8920; Consultado en: 2022/04/02/00:14:42. Disponible en: https://doi.org/10.2166/wh.2021.011. Disponible en: 10.2166/wh.2021.011.Jinatham, Vasana; Maxamhud, Sadiya; Popluechai, Siam; Tsaousis, Anastasios D.; Gentekaki, Eleni (2021) Blastocystis One Health Approach in a Rural Community of Northern Thailand: Prevalence, Subtypes and Novel Transmission Routes. En: Frontiers in Microbiology. Vol. 12; 1664-302X; Consultado en: 2022/06/30/22:42:29. Disponible en: https://www.frontiersin.org/article/10.3389/fmicb.2021.746340.Clark, C. Graham; van der Giezen, Mark; Alfellani, Mohammed A.; Stensvold, C. Rune; Rollinson, D. (2013) Chapter One. En: Advances in Parasitology. Vol. 82; pp. 1 - 32; Academic Press; Consultado en: 2022/07/07/01:35:11. Disponible en: https://www.sciencedirect.com/science/article/pii/B9780124077065000010.Baek, Sohyeong; Maloney, Jenny G.; Molokin, Aleksey; George, Nadja S.; Cortés Vecino, Jesús A.; Santin, Monica (2022) Diversity of Blastocystis Subtypes in Horses in Colombia and Identification of Two New Subtypes. En: Microorganisms. Vol. 10; No. 9; pp. 1693 2076-2607; Consultado en: 2022/10/07/16:43:23. Disponible en: https://www.mdpi.com/2076-2607/10/9/1693. Disponible en: 10.3390/microorganisms10091693.Wylezich, Claudia; Belka, Ariane; Hanke, Dennis; Beer, Martin; Blome, Sandra; Höper, Dirk (2019) Metagenomics for broad and improved parasite detection: a proof-of-concept study using swine faecal samples. En: International Journal for Parasitology. Vol. 49; No. 10; pp. 769 - 777; 0020-7519; Consultado en: 2023/03/20/04:14:20. Disponible en: https://www.sciencedirect.com/science/article/pii/S0020751919301869. Disponible en: 10.1016/j.ijpara.2019.04.007.AbuOdeh, Raed; Ezzedine, Sinda; Madkour, Mohamed; Stensvold, Christen Rune; Samie, Amidou; Nasrallah, Gheyath; AlAbsi, Enas; ElBakri, Ali (2019) Molecular Subtyping of Blastocystis from Diverse Animals in the United Arab Emirates. En: Protist. Vol. 170; No. 5; pp. 125679 1434-4610; Consultado en: 2023/03/20/07:05:51. Disponible en: https://www.sciencedirect.com/science/article/pii/S1434461019300094. Disponible en: 10.1016/j.protis.2019.125679.Onder, Zuhal; Yildirim, Alparslan; Pekmezci, Didem; Duzlu, Onder; Pekmezci, Gokmen Zafer; Ciloglu, Arif; Simsek, Emrah; Kokcu, Nesrin Delibasi; Yetismis, Gamze; Ercan, Nuri; Inci, Abdullah (2021) Molecular identification and subtype distribution of Blastocystis sp. in farm and pet animals in Turkey. En: Acta Tropica. Vol. 220; pp. 105939 0001-706X; Consultado en: 2023/03/20/07:10:55. Disponible en: https://www.sciencedirect.com/science/article/pii/S0001706X21001182. Disponible en: 10.1016/j.actatropica.2021.105939.Udonsom, Ruenruetai; Prasertbun, Rapeepan; Mahittikorn, Aongart; Mori, Hirotake; Changbunjong, Tanasak; Komalamisra, Chalit; Pintong, Ai-rada; Sukthana, Yaowalark; Popruk, Supaluk (2018) Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand. En: Infection, Genetics and Evolution. Vol. 65; pp. 107 - 111; 1567-1348; Consultado en: 2023/03/20/08:25:06. Disponible en: https://www.sciencedirect.com/science/article/pii/S1567134818304805. Disponible en: 10.1016/j.meegid.2018.07.007.Jiménez, Paula A.; Munoz, Marina; Ramirez, Juan David; An update on the distribution of Blastocystis subtypes in the Americas. Consultado en: 2023/03/20/10:54:44. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816782/.Salehi, Roya; Rostami, Ali; Mirjalali, Hamed; Stensvold, Christen Rune; Haghighi, Ali (2022) Genetic characterization of Blastocystis from poultry, livestock animals and humans in the southwest region of Iran—Zoonotic implications. En: Transboundary and Emerging Diseases. Vol. 69; No. 3; pp. 1178 - 1185;Maloney, Jenny G.; Molokin, Aleksey; Seguí, Raimundo; Maravilla, Pablo; Martínez-Hernández, Fernando; Villalobos, Guiehdani; Tsaousis, Anastasios D.; Gentekaki, Eleni; Muñoz-Antolí, Carla; Klisiowicz, Debora R.; Oishi, Camila Y.; Toledo, Rafael; Esteban, J. Guillermo; Köster, Pamela C.; de Lucio, Aida; Dashti, Alejandro; Bailo, Begoña; Calero-Bernal, Rafael; González-Barrio, David; Carmena, David; Santín, Mónica (2023) Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38. En: Microorganisms. Vol. 11; No. 1; pp. 46 2076-2607; Consultado en: 2023/08/10/08:12:44. Disponible en: https://www.mdpi.com/2076-2607/11/1/46. Disponible en: 10.3390/microorganisms11010046.Rudzińska, Monika; Kowalewska, Beata; Kurpas, Monika; Szostakowska, Beata (2022) Rare Occurrence of Blastocystis in Pet Animals and Their Owners in the Pomeranian Voivodeship in Poland in the Light of Literature Data. En: Journal of Clinical Medicine. Vol. 11; No. 11; pp. 2975 2077-0383; Consultado en: 2023/08/10/09:09:54. Disponible en: https://www.mdpi.com/2077-0383/11/11/2975. Disponible en: 10.3390/jcm11112975.Rauff-Adedotun, Adedolapo Aminat; Mohd Zain, Siti Nursheena; Farah Haziqah, Meor Termizi (2020) Current status of Blastocystis sp. in animals from Southeast Asia: a review. En: Parasitology Research. Vol. 119; No. 11; pp. 3559 - 3570; 1432-1955; Consultado en: 2023/08/10/13:16:50. Disponible en: https://doi.org/10.1007/s00436-020-06828-8. Disponible en: 10.1007/s00436-020-06828-8.Naguib, Doaa; Gantois, Nausicaa; Desramaut, Jeremy; Arafat, Nagah; Even, Gaël; Certad, Gabriela; Chabé, Magali; Viscogliosi, Eric (2022) Prevalence, Subtype Distribution and Zoonotic Significance of Blastocystis sp. Isolates from Poultry, Cattle and Pets in Northern Egypt. En: Microorganisms. Vol. 10; No. 11; pp. 2259 2076-2607; Consultado en: 2023/08/10/13:26:41. Disponible en: https://www.mdpi.com/2076-2607/10/11/2259. Disponible en: 10.3390/microorganisms10112259.Russini, Valeria; Di Filippo, Margherita Montalbano; Fanelli, Rita; Polidori, Miriam; Berrilli, Federica; Di Cave, David; Novelletto, Andrea; Calderini, Pietro (2020) Characterization of prevalence and genetic subtypes of Blastocystis sp. in wild and domestic Suidae of central Italy aided by amplicon NGS. En: Veterinary Parasitology: Regional Studies and Reports. Vol. 22; pp. 100472 2405-9390; Consultado en: 2023/08/10/13:52:18. Disponible en: https://www.sciencedirect.com/science/article/pii/S2405939020302537. Disponible en: 10.1016/j.vprsr.2020.100472.Asghari, Ali; Sadrebazzaz, Alireza; Shamsi, Laya; Shams, Morteza (2021) Global prevalence, subtypes distribution, zoonotic potential, and associated risk factors of Blastocystis sp. in domestic pigs (Sus domesticus) and wild boars (Sus scrofa): A systematic review and meta-analysis. En: Microbial Pathogenesis. Vol. 160; pp. 105183 0882-4010; Consultado en: 2023/08/10/13:52:55. Disponible en: https://www.sciencedirect.com/science/article/pii/S0882401021004575. Disponible en: 10.1016/j.micpath.2021.105183.Chen, Hongshuang; Hao, Yaru; Liu, Yuqing; Xu, Mingqi; Zhang, Weizhe; Li, He; Yang, Fengkun (2023) The frequency and subtype distribution of Blastocystis sp. in humans and domestic animals in households in Heilongjiang Province, China. En: Acta Tropica. Vol. 240; pp. 106844 0001-706X; Consultado en: 2023/08/10/14:24:38. Disponible en: https://www.sciencedirect.com/science/article/pii/S0001706X23000311. Disponible en: 10.1016/j.actatropica.2023.106844.Li, Junqiang; Karim, Md. Robiul; Li, Dongfang; Rahaman Sumon, S. M. Mostafizur; Siddiki, S. H. M. Faruk; Rume, Farzana Islam; Sun, Ruiqin; Jia, Yaquan; Zhang, Longxian (2019) Molecular characterization of Blastocystis sp. in captive wildlife in Bangladesh National Zoo: Non-human primates with high prevalence and zoonotic significance. En: International Journal for Parasitology: Parasites and Wildlife. Vol. 10; pp. 314 - 320; 2213-2244; Consultado en: 2023/08/10/14:27:01. Disponible en: https://www.sciencedirect.com/science/article/pii/S2213224419302378. Disponible en: 10.1016/j.ijppaw.2019.11.003.Rauff-Adedotun, Adedolapo Aminat; Lee, Ii Li; Abd Talib, Nabilah; Shaari, Nurshafarina; Yahaya, Zary Shariman; Meor Termizi, Farah Haziqah (2023) Prevalence, potential risk factors and genetic diversity of Blastocystis in ruminant livestock animals from Penang, Malaysia. En: Parasitology Research. 1432-1955; Consultado en: 2023/08/10/14:27:17. Disponible en: https://doi.org/10.1007/s00436-023-07920-5. Disponible en: 10.1007/s00436-023-07920-5.Poulsen, Casper S.; Efunshile, Akinwale M.; Nelson, Jenna A.; Stensvold, Christen R. (2016) Epidemiological Aspects of Blastocystis Colonization in Children in Ilero, Nigeria. En: The American Journal of Tropical Medicine and Hygiene. Vol. 95; No. 1; pp. 175 - 179; 0002-9637; Consultado en: 2023/08/10/14:36:02. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4944538/. Disponible en: 10.4269/ajtmh.16-0074.Munsaka, Gilbert; Hayashida, Kyoko; Mubemba, Benjamin; Simulundu, Edgar; Mulunda, Namwiinga; Pule, Ruth; Sianongo, Sandie; Makuluni, Marina; Muleya, Walter; Changula, Katendi; Chitanga, Simbarashe; Mutengo, Mable (2022) Molecular subtyping of Blastocystis sp. detected in patients at a large tertiary referral hospital in Lusaka, Zambia. En: Frontiers in Parasitology. Vol. 1; 2813-2424; Consultado en: 2023/08/10/14:36:13. Disponible en: https://www.frontiersin.org/articles/10.3389/fpara.2022.1033485.Ahmed, Shahira A.; El-Mahallawy, Heba S.; Mohamed, Samar Farag; Angelici, Maria Cristina; Hasapis, Kyriacos; Saber, Taisir; Karanis, Panagiotis (2022) Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt. En: Scientific Reports. Vol. 12; No. 1; pp. 19084 2045-2322; Consultado en: 2023/08/10/14:40:58. Disponible en: https://www.nature.com/articles/s41598-022-23360-0. Disponible en: 10.1038/s41598-022-23360-0.Deng, Lei; Wojciech, Lukasz; Png, Chin Wen; Kioh, Dorinda Yan Qin; Gu, Yuxiang; Aung, Thet Tun; Malleret, Benoit; Chan, Eric Chun Yong; Peng, Guangneng; Zhang, Yongliang; Gascoigne, Nicholas Robert John; Tan, Kevin Shyong Wei (2023) Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. En: Theranostics. Vol. 13; No. 3; pp. 1165 - 1179; 1838-7640; Disponible en: 10.7150/thno.81583.Danišová, Oľga; Valenčáková, Alexandra (2021) First detection of Blastocystis sp. in pigs in Slovakia and in Europe. En: Parasitology International. Vol. 81; pp. 102235 1383-5769; Consultado en: 2023/08/10/14:59:06. Disponible en: https://www.sciencedirect.com/science/article/pii/S1383576920301859. Disponible en: 10.1016/j.parint.2020.102235.Pawestri, A. R.; Padungsombat, J.; Deesitthivech, O.; Nakayama, E.; Shioda, T.; Moonsom, S. (2022) Blastocystis spp. Subtype Distribution from Human and Animals at the Thai-Myanmar Border: The Public Health Implication. En: International Journal of Infectious Diseases. Abstracts from the Eighth International Meeting on Emerging Diseases and Surveillance, IMED 2021; Vol. 116; pp. S80 1201-9712; Consultado en: 2023/09/06/09:32:26. Disponible en: https://www.sciencedirect.com/science/article/pii/S1201971221010821. Disponible en: 10.1016/j.ijid.2021.12.190.Santin, Monica; Figueiredo, Ana; Molokin, Aleksey; George, Nadja S; Köster, Pamela C.; Dashti, Alejandro; González-Barrio, David; Carmena, David; Maloney, Jenny G.; Division of Blastocystis ST10 into three new subtypes: ST42-ST44. En: Journal of Eukaryotic Microbiology. Vol. n/a; No. n/a; pp. e12998 1550-7408; Consultado en: 2023/09/11/06:25:04. Disponible en: https://onlinelibrary.wiley.com/doi/abs/10.1111/jeu.12998. Disponible en: 10.1111/jeu.12998.Stensvold, Christen Rune; Berg, Rebecca P. K. D.; Maloney, Jenny G.; Molokin, Aleksey; Santin, Monica (2023) Molecular characterization of Blastocystis and Entamoeba of muskoxen and sheep in Greenland. En: International Journal for Parasitology. Vol. 53; No. 11-12; pp. 673 - 685; 1879-0135; Disponible en: 10.1016/j.ijpara.2023.05.005.Hernández-Castro, Carolina; Maloney, Jenny G.; Agudelo-López, Sonia P.; Toro-Londoño, Miguel A.; Botero-Garcés, Jorge H.; Orozco, María C.; Quintero-Quinchia, Yulieth C.; Correa-Cote, Juan C.; Múnera-Duque, Alejandro; Ricaurte-Ciro, Juan C.; Londoño-Álvarez, Luis I.; Escobar, René M.; Köster, Pamela C.; Sánchez, Sergio; Carmena, David; Santín, Mónica (2023) Identification and validation of novel Blastocystis subtype ST41 in a Colombian patient undergoing colorectal cancer screening. En: The Journal of Eukaryotic Microbiology. Vol. 70; No. 5; pp. e12978 1550-7408; Disponible en: 10.1111/jeu.12978.Rondón, Silvia; Cavallero, Serena; Link, Andrés; González, Camila; D’Amelio, Stefano (2023) Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia. En: Pathogens. Vol. 12; No. 4; pp. 569 2076-0817; Consultado en: 2023/09/11/06:28:16. Disponible en: https://www.mdpi.com/2076-0817/12/4/569. Disponible en: 10.3390/pathogens12040569.Cruz-Saavedra, Lissa; Arévalo, Vivian Alejandra; Garcia-Corredor, Diego; Jiménez, Paula Andrea; Vega, Laura; Pulido-Medellín, Martín; Ortiz-Pineda, Melissa; Ramírez, Juan David (2023) Molecular detection and characterization of Giardia spp., Cryptosporidium spp., and Blastocystis in captive wild animals rescued from central Colombia. En: International Journal for Parasitology: Parasites and Wildlife. Vol. 22; pp. 1 - 5; 2213-2244; Disponible en: https://www.sciencedirect.com/science/article/pii/S2213224423000573. Disponible en: https://doi.org/10.1016/j.ijppaw.2023.07.005.Hernández, Paula C.; Maloney, Jenny G.; Molokin, Aleksey; George, Nadja S.; Morales, Liliana; Chaparro-Olaya, Jacqueline; Santin, Monica (2023) Exploring Blastocystis genetic diversity in rural schoolchildren from Colombia using next-generation amplicon sequencing reveals significant associations between contact with animals and infection risk. En: Parasitology Research. Vol. 122; No. 7; pp. 1451 - 1462; 1432-1955; Consultado en: 2023/10/22/10:09:27. Disponible en: https://doi.org/10.1007/s00436-023-07841-3. Disponible en: 10.1007/s00436-023-07841-3.Mokhtar, Amira; Youssef, Ahmed (2018) Subtype analysis of Blastocystis spp. isolated from domestic mammals and poultry and its relation to transmission to their in-contact humans in Ismailia governorate, Egypt. En: Parasitologists United Journal. Vol. 11; No. 2; pp. 90 - 98;Paulos, Silvia; Köster, Pamela C.; de Lucio, Aida; Hernández-de-Mingo, Marta; Cardona, Guillermo A.; Fernández-Crespo, Juan C.; Stensvold, Christen R.; Carmena, David (2018) Occurrence and subtype distribution of Blastocystis sp. in humans, dogs and cats sharing household in northern Spain and assessment of zoonotic transmission risk. En: Zoonoses and Public Health. Vol. 65; No. 8; pp. 993 - 1002; 1863-2378; Disponible en: 10.1111/zph.12522.Potes-Morales, Caterine; Crespo-Ortiz, Maria del Pilar (2023) Molecular diagnosis of intestinal protozoa in young adults and their pets in Colombia, South America. En: PLOS ONE. Vol. 18; No. 5; pp. e0283824 1932-6203; Consultado en: 2023/11/05/21:45:49. Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0283824. Disponible en: 10.1371/journal.pone.0283824.Mahdavi, Farzad; Asghari, Ali; Shahabi, Saeed; Shamsi, Laya; Soltani-Jazi, Farzaneh; Sadrebazzaz, Alireza; Shams, Morteza (2022) Distribution, genetic diversity, and zoonotic significance of Blastocystis subtypes in pet dogs. En: Comparative Immunology, Microbiology and Infectious Diseases. Vol. 88; pp. 101848 0147-9571; Consultado en: 2023/11/05/21:48:35. Disponible en: https://www.sciencedirect.com/science/article/pii/S0147957122001059. Disponible en: 10.1016/j.cimid.2022.101848.Huson, Daniel H.; Bryant, David (2006) Application of phylogenetic networks in evolutionary studies. En: Molecular Biology and Evolution. Vol. 23; No. 2; pp. 254 - 267; 0737-4038; Disponible en: 10.1093/molbev/msj030.Xu, Qingbiao; Qiao, Qinqin; Gao, Ya; Hou, Jinxiu; Hu, Mingyang; Du, Yufeng; Zhao, Ke; Li, Xiang (2021) Gut Microbiota and Their Role in Health and Metabolic Disease of Dairy Cow. En: Frontiers in Nutrition. Vol. 8; 2296-861X; Consultado en: 2023/11/06/06:32:43. Disponible en: https://www.frontiersin.org/articles/10.3389/fnut.2021.701511.Rudzińska, Monika; Sikorska, Katarzyna (2023) Epidemiology of Blastocystis Infection: A Review of Data from Poland in Relation to Other Reports. En: Pathogens. Vol. 12; No. 8; pp. 1050 2076-0817; Consultado en: 2023/11/06/12:29:36. Disponible en: https://www.mdpi.com/2076-0817/12/8/1050. Disponible en: 10.3390/pathogens12081050.Delahaye, Clara; Nicolas, Jacques (2021) Sequencing DNA with nanopores: Troubles and biases. En: PLOS ONE. Vol. 16; No. 10; pp. e0257521 1932-6203; Consultado en: 2023/11/24/07:28:32. Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0257521. Disponible en: 10.1371/journal.pone.0257521.Sahlin, Kristoffer; Medvedev, Paul (2021) Error correction enables use of Oxford Nanopore technology for reference-free transcriptome analysis. En: Nature Communications. Vol. 12; No. 1; pp. 2 2041-1723; Consultado en: 2023/11/24/13:21:47. Disponible en: https://www.nature.com/articles/s41467-020-20340-8. 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Diversidad genética de Blastocystis en muestras animales y humanas de varios departamentos de Colombia empleando secuenciación completa del gen 18S rRNA (SSU rRNA) por Oxford Nanopore Technologies (ONT)

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