Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice
Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2014
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/18701
- Acceso en línea:
- https://doi.org/10.1371/journal.pone.0100853
http://repository.urosario.edu.co/handle/10336/18701
- Palabra clave:
- Glutatión transferasa A2
Mensajero RNA
Retinol
Factor de transcripción Nrf2
Distribución de edad
Envejecido
Experimento con animales
Modelo animal
Tejido animal
Peso corporal
Estudio controlado
Cyp4A14 gen D
Restricción de la dieta
Oxidación de ácidos grasos
Gen
Perfiles de expresión génica
Gen Gsta2
Histopatología
Función del riñón
Lesión renal
Isquemia renal
Necrosis del túbulo renal
Secuencia de nucleótidos
Obesidad
Período preoperatorio
Protección Renal
Lesión por reperfusión
Gen Sc4Mol
Estrés
Tasa de supervivencia
Regeneración de Tejidos
Nivel de urea en sangre
Metabolismo de las vitaminas
Efectos adversos
Ratón C57Bl
Trasplante de riñón
Metabolismo
Obesidad
Estrés oxidativo
Patología
Lesión por reperfusión
Nivel de sangre de nitrógeno ureico
Factores de edad
Nitrógeno ureico en sangre
Redes y vías metabólicas
Ratones endogámicos C57Bl
Exceso de peso
Estrés oxidativo
Lesión por reperfusión
Reperfusion Injury
Oxidative Stress
Mice, Inbred C57Bl
Overweight
Blood Urea Nitrogen
Metabolic Networks And Pathways
Age Factors
Urea Nitrogen Blood Level
Reperfusion Injury
Pathology
Oxidative Stress
Obesity
Kidney Transplantation
Metabolism
C57Bl Mouse
Adverse Effects
Vitamin Metabolism
Urea Blood Level
Tissue Regeneration
Survival Rate
Stress
Sc4Mol Gene
Reperfusion Injury
Preoperative Period
Renal Protection
Obesity
Nucleotide Sequene
Kidney Tubule Necrosis
Kidney Ischemia
Kidney Injury
Kidney Function
Histopathology
Gsta2 Gene
Gene Expression Profiling
Gene
Fatty Acid Oxidation
Diet Restriction
Cyp4A14 Dgene
Controlled Study
Body Weight
Animal Tissue
Animal Model
Animal Experiment
Aged
Age Distribution
Urea
Transcription Factor Nrf2
Messenger Rna
Glutathione Transferase A2
Trasplante de riñon
Isquemia
Reperfusión
- Rights
- License
- Abierto (Texto Completo)
id |
EDOCUR2_d1170e71cb85cb545b1d16ec8d3c51ad |
---|---|
oai_identifier_str |
oai:repository.urosario.edu.co:10336/18701 |
network_acronym_str |
EDOCUR2 |
network_name_str |
Repositorio EdocUR - U. Rosario |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
title |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
spellingShingle |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice Glutatión transferasa A2 Mensajero RNA Retinol Factor de transcripción Nrf2 Distribución de edad Envejecido Experimento con animales Modelo animal Tejido animal Peso corporal Estudio controlado Cyp4A14 gen D Restricción de la dieta Oxidación de ácidos grasos Gen Perfiles de expresión génica Gen Gsta2 Histopatología Función del riñón Lesión renal Isquemia renal Necrosis del túbulo renal Secuencia de nucleótidos Obesidad Período preoperatorio Protección Renal Lesión por reperfusión Gen Sc4Mol Estrés Tasa de supervivencia Regeneración de Tejidos Nivel de urea en sangre Metabolismo de las vitaminas Efectos adversos Ratón C57Bl Trasplante de riñón Metabolismo Obesidad Estrés oxidativo Patología Lesión por reperfusión Nivel de sangre de nitrógeno ureico Factores de edad Nitrógeno ureico en sangre Redes y vías metabólicas Ratones endogámicos C57Bl Exceso de peso Estrés oxidativo Lesión por reperfusión Reperfusion Injury Oxidative Stress Mice, Inbred C57Bl Overweight Blood Urea Nitrogen Metabolic Networks And Pathways Age Factors Urea Nitrogen Blood Level Reperfusion Injury Pathology Oxidative Stress Obesity Kidney Transplantation Metabolism C57Bl Mouse Adverse Effects Vitamin Metabolism Urea Blood Level Tissue Regeneration Survival Rate Stress Sc4Mol Gene Reperfusion Injury Preoperative Period Renal Protection Obesity Nucleotide Sequene Kidney Tubule Necrosis Kidney Ischemia Kidney Injury Kidney Function Histopathology Gsta2 Gene Gene Expression Profiling Gene Fatty Acid Oxidation Diet Restriction Cyp4A14 Dgene Controlled Study Body Weight Animal Tissue Animal Model Animal Experiment Aged Age Distribution Urea Transcription Factor Nrf2 Messenger Rna Glutathione Transferase A2 Trasplante de riñon Isquemia Reperfusión |
title_short |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
title_full |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
title_fullStr |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
title_full_unstemmed |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
title_sort |
Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice |
dc.subject.spa.fl_str_mv |
Glutatión transferasa A2 Mensajero RNA Retinol Factor de transcripción Nrf2 Distribución de edad Envejecido Experimento con animales Modelo animal Tejido animal Peso corporal Estudio controlado Cyp4A14 gen D Restricción de la dieta Oxidación de ácidos grasos Gen Perfiles de expresión génica Gen Gsta2 Histopatología Función del riñón Lesión renal Isquemia renal Necrosis del túbulo renal Secuencia de nucleótidos Obesidad Período preoperatorio Protección Renal Lesión por reperfusión Gen Sc4Mol Estrés Tasa de supervivencia Regeneración de Tejidos Nivel de urea en sangre Metabolismo de las vitaminas Efectos adversos Ratón C57Bl Trasplante de riñón Metabolismo Obesidad Estrés oxidativo Patología Lesión por reperfusión Nivel de sangre de nitrógeno ureico Factores de edad Nitrógeno ureico en sangre Redes y vías metabólicas Ratones endogámicos C57Bl Exceso de peso Estrés oxidativo Lesión por reperfusión |
topic |
Glutatión transferasa A2 Mensajero RNA Retinol Factor de transcripción Nrf2 Distribución de edad Envejecido Experimento con animales Modelo animal Tejido animal Peso corporal Estudio controlado Cyp4A14 gen D Restricción de la dieta Oxidación de ácidos grasos Gen Perfiles de expresión génica Gen Gsta2 Histopatología Función del riñón Lesión renal Isquemia renal Necrosis del túbulo renal Secuencia de nucleótidos Obesidad Período preoperatorio Protección Renal Lesión por reperfusión Gen Sc4Mol Estrés Tasa de supervivencia Regeneración de Tejidos Nivel de urea en sangre Metabolismo de las vitaminas Efectos adversos Ratón C57Bl Trasplante de riñón Metabolismo Obesidad Estrés oxidativo Patología Lesión por reperfusión Nivel de sangre de nitrógeno ureico Factores de edad Nitrógeno ureico en sangre Redes y vías metabólicas Ratones endogámicos C57Bl Exceso de peso Estrés oxidativo Lesión por reperfusión Reperfusion Injury Oxidative Stress Mice, Inbred C57Bl Overweight Blood Urea Nitrogen Metabolic Networks And Pathways Age Factors Urea Nitrogen Blood Level Reperfusion Injury Pathology Oxidative Stress Obesity Kidney Transplantation Metabolism C57Bl Mouse Adverse Effects Vitamin Metabolism Urea Blood Level Tissue Regeneration Survival Rate Stress Sc4Mol Gene Reperfusion Injury Preoperative Period Renal Protection Obesity Nucleotide Sequene Kidney Tubule Necrosis Kidney Ischemia Kidney Injury Kidney Function Histopathology Gsta2 Gene Gene Expression Profiling Gene Fatty Acid Oxidation Diet Restriction Cyp4A14 Dgene Controlled Study Body Weight Animal Tissue Animal Model Animal Experiment Aged Age Distribution Urea Transcription Factor Nrf2 Messenger Rna Glutathione Transferase A2 Trasplante de riñon Isquemia Reperfusión |
dc.subject.keyword.spa.fl_str_mv |
Reperfusion Injury Oxidative Stress Mice, Inbred C57Bl |
dc.subject.keyword.eng.fl_str_mv |
Overweight Blood Urea Nitrogen Metabolic Networks And Pathways Age Factors Urea Nitrogen Blood Level Reperfusion Injury Pathology Oxidative Stress Obesity Kidney Transplantation Metabolism C57Bl Mouse Adverse Effects Vitamin Metabolism Urea Blood Level Tissue Regeneration Survival Rate Stress Sc4Mol Gene Reperfusion Injury Preoperative Period Renal Protection Obesity Nucleotide Sequene Kidney Tubule Necrosis Kidney Ischemia Kidney Injury Kidney Function Histopathology Gsta2 Gene Gene Expression Profiling Gene Fatty Acid Oxidation Diet Restriction Cyp4A14 Dgene Controlled Study Body Weight Animal Tissue Animal Model Animal Experiment Aged Age Distribution Urea Transcription Factor Nrf2 Messenger Rna Glutathione Transferase A2 |
dc.subject.lemb.spa.fl_str_mv |
Trasplante de riñon Isquemia Reperfusión |
description |
Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a different response to fasting, we investigated the effects of preoperative fasting on renal IRI in aged-overweight male and female mice. Male and female F1-FVB/C57BL6-hybrid mice, average age 73 weeks weighing 47.2 grams, were randomized to preoperative ad libitum feeding or 3 days fasting, followed by renal IRI. Body weight, kidney function and survival of the animals were monitored until day 28 postoperatively. Kidney histopathology was scored for all animals and gene expression profiles after fasting were analyzed in kidneys of young and aged male mice. Preoperative fasting significantly improved survival after renal IRI in both sexes compared with normal fed mice. Fasted groups had a better kidney function shown by lower serum urea levels after renal IRI. Histopathology showed less acute tubular necrosis and more regeneration in kidneys from fasted mice. A mRNA analysis indicated the involvement of metabolic processes including fatty acid oxidation and retinol metabolism, and the NRF2-mediated stress response. Similar to young-lean, healthy male mice, preoperative fasting protects against renal IRI in aged-overweight mice of both genders. These findings suggest a general protective response of fasting against renal IRI regardless of age, gender, body weight and genetic background. Therefore, fasting could be a non-invasive intervention inducing increased oxidative stress resistance in older and overweight patients as well. © 2014 Jongbloed et al. |
publishDate |
2014 |
dc.date.created.none.fl_str_mv |
2014 |
dc.date.issued.none.fl_str_mv |
2014 |
dc.date.accessioned.none.fl_str_mv |
2018-11-19T15:04:32Z |
dc.date.available.none.fl_str_mv |
2018-11-19T15:04:32Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1371/journal.pone.0100853 |
dc.identifier.issn.none.fl_str_mv |
ISSN 1932-6203 |
dc.identifier.uri.none.fl_str_mv |
http://repository.urosario.edu.co/handle/10336/18701 |
url |
https://doi.org/10.1371/journal.pone.0100853 http://repository.urosario.edu.co/handle/10336/18701 |
identifier_str_mv |
ISSN 1932-6203 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 6 |
dc.relation.citationTitle.none.fl_str_mv |
PLoS ONE |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 9 |
dc.relation.ispartof.spa.fl_str_mv |
PLoS ONE, ISSN: 1932-6203, Vol. 9/No. 6 (2014) |
dc.relation.uri.spa.fl_str_mv |
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0100853&type=printable |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
dc.rights.cc.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
rights_invalid_str_mv |
Abierto (Texto Completo) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
dc.source.bibliographicCitation.spa.fl_str_mv |
Tonelli, M., Wiebe, N., Knoll, G., Bello, A., Browne, S., Systematic review: Kidney transplantation compared with dialysis in clinically relevant outcomes (2011) Am J Transplant, 11, pp. 2093-2109 |
dc.source.instname.none.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
bitstream.url.fl_str_mv |
https://repository.urosario.edu.co/bitstreams/35529d8a-2e23-4c58-9747-20d399bc27fe/download https://repository.urosario.edu.co/bitstreams/530e7e3b-df64-452f-8453-aa40679aad16/download https://repository.urosario.edu.co/bitstreams/695599f6-7822-421c-ae56-d8683bd2176b/download |
bitstream.checksum.fl_str_mv |
3d740aaf2ebd0ba2ea95ee74e66ac216 6b1a2b97bc618e6960f96cc35bc5fd8b d56f1aef533ef7e4eb36f937cd8f6353 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1808390889414852608 |
spelling |
7d982a7f-c9a4-4572-b0ad-907d4137ad956004e754d08-87e6-4ed1-841a-d77454cf63d36000a29d219-2ce9-4403-a734-dc125a094aa16007632723360084f10b7b-6e0f-4d41-b26f-98b7e6a94c48600f4e00199-5620-4c18-aace-3f0b8ff96605600ee9e7e15-5b92-4393-8de1-d8a661f5c6d760022d7b8d2-0adb-4ad5-b752-5563bbd8b8b86005c377a11-8f57-461d-a797-400f948160c66003fece108-fdb3-49b5-89e9-efd05c0e0071600b8315f72-c569-46c2-bcf3-cac629764d556002018-11-19T15:04:32Z2018-11-19T15:04:32Z20142014Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a different response to fasting, we investigated the effects of preoperative fasting on renal IRI in aged-overweight male and female mice. Male and female F1-FVB/C57BL6-hybrid mice, average age 73 weeks weighing 47.2 grams, were randomized to preoperative ad libitum feeding or 3 days fasting, followed by renal IRI. Body weight, kidney function and survival of the animals were monitored until day 28 postoperatively. Kidney histopathology was scored for all animals and gene expression profiles after fasting were analyzed in kidneys of young and aged male mice. Preoperative fasting significantly improved survival after renal IRI in both sexes compared with normal fed mice. Fasted groups had a better kidney function shown by lower serum urea levels after renal IRI. Histopathology showed less acute tubular necrosis and more regeneration in kidneys from fasted mice. A mRNA analysis indicated the involvement of metabolic processes including fatty acid oxidation and retinol metabolism, and the NRF2-mediated stress response. Similar to young-lean, healthy male mice, preoperative fasting protects against renal IRI in aged-overweight mice of both genders. These findings suggest a general protective response of fasting against renal IRI regardless of age, gender, body weight and genetic background. Therefore, fasting could be a non-invasive intervention inducing increased oxidative stress resistance in older and overweight patients as well. © 2014 Jongbloed et al.application/pdfhttps://doi.org/10.1371/journal.pone.0100853ISSN 1932-6203http://repository.urosario.edu.co/handle/10336/18701engNo. 6PLoS ONEVol. 9PLoS ONE, ISSN: 1932-6203, Vol. 9/No. 6 (2014)https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0100853&type=printableAbierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2Tonelli, M., Wiebe, N., Knoll, G., Bello, A., Browne, S., Systematic review: Kidney transplantation compared with dialysis in clinically relevant outcomes (2011) Am J Transplant, 11, pp. 2093-2109instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURGlutatión transferasa A2Mensajero RNARetinolFactor de transcripción Nrf2Distribución de edadEnvejecidoExperimento con animalesModelo animalTejido animalPeso corporalEstudio controladoCyp4A14 gen DRestricción de la dietaOxidación de ácidos grasosGenPerfiles de expresión génicaGen Gsta2HistopatologíaFunción del riñónLesión renalIsquemia renalNecrosis del túbulo renalSecuencia de nucleótidosObesidadPeríodo preoperatorioProtección RenalLesión por reperfusiónGen Sc4MolEstrésTasa de supervivenciaRegeneración de TejidosNivel de urea en sangreMetabolismo de las vitaminasEfectos adversosRatón C57BlTrasplante de riñónMetabolismoObesidadEstrés oxidativoPatologíaLesión por reperfusiónNivel de sangre de nitrógeno ureicoFactores de edadNitrógeno ureico en sangreRedes y vías metabólicasRatones endogámicos C57BlExceso de pesoEstrés oxidativoLesión por reperfusiónReperfusion InjuryOxidative StressMice, Inbred C57BlOverweightBlood Urea NitrogenMetabolic Networks And PathwaysAge FactorsUrea Nitrogen Blood LevelReperfusion InjuryPathologyOxidative StressObesityKidney TransplantationMetabolismC57Bl MouseAdverse EffectsVitamin MetabolismUrea Blood LevelTissue RegenerationSurvival RateStressSc4Mol GeneReperfusion InjuryPreoperative PeriodRenal ProtectionObesityNucleotide SequeneKidney Tubule NecrosisKidney IschemiaKidney InjuryKidney FunctionHistopathologyGsta2 GeneGene Expression ProfilingGeneFatty Acid OxidationDiet RestrictionCyp4A14 DgeneControlled StudyBody WeightAnimal TissueAnimal ModelAnimal ExperimentAgedAge DistributionUreaTranscription Factor Nrf2Messenger RnaGlutathione Transferase A2Trasplante de riñonIsquemiaReperfusiónPreoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight micearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Jongbloed, Frannyde Bruin, Ron W. F.Pennings, Jeroen L. A.Payan-Gomez, CesarEngel, Sandra van denOostrom, Conny T. vande Bruin, AlainHoeijmakers, Jan H. J.Steeg, Harry vanIJzermans, Jan N. M.Dollé, Martijn E. T.Jongbloed, Frannyde Bruin, Ron W. F.Pennings, Jeroen L. A.Payán-Gómez, CésarEngel, Sandra van denOostrom, Conny T. vande Bruin, AlainHoeijmakers, Jan H. J.Steeg, Harry vanIJzermans, Jan N. M.Dollé, Martijn E. T.ORIGINAL113.pdfapplication/pdf757338https://repository.urosario.edu.co/bitstreams/35529d8a-2e23-4c58-9747-20d399bc27fe/download3d740aaf2ebd0ba2ea95ee74e66ac216MD51TEXT113.pdf.txt113.pdf.txtExtracted texttext/plain48202https://repository.urosario.edu.co/bitstreams/530e7e3b-df64-452f-8453-aa40679aad16/download6b1a2b97bc618e6960f96cc35bc5fd8bMD52THUMBNAIL113.pdf.jpg113.pdf.jpgGenerated Thumbnailimage/jpeg4835https://repository.urosario.edu.co/bitstreams/695599f6-7822-421c-ae56-d8683bd2176b/downloadd56f1aef533ef7e4eb36f937cd8f6353MD5310336/18701oai:repository.urosario.edu.co:10336/187012022-08-29 14:19:06.685https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |