Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme
Candida albicans is a fungal opportunistic pathogen of significant public health importance mainly due to the recent emergence of strains with increased aggressiveness and antifungal resistance. Here, we aimed to describe the epidemiological profiles and approximate the population structure of C. al...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2019
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/24093
- Acceso en línea:
- https://doi.org/10.3389/fmicb.2019.01052
https://repository.urosario.edu.co/handle/10336/24093
- Palabra clave:
- Adult
Article
Bootstrapping
Candida albicans
Chromosome
Controlled study
Diploidy
Epidemiological monitoring
Female
Genetic variability
Housekeeping gene
Human
Human experiment
Major clinical study
Molecular epidemiology
Multilocus sequence typing
Nonhuman
Population genetic structure
Vagina
Validation process
Candida albicans
Molecular epidemiology
Multilocus sequence typing
Population genetic structure
Recombination
- Rights
- License
- Abierto (Texto Completo)
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dc.title.spa.fl_str_mv |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
title |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
spellingShingle |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme Adult Article Bootstrapping Candida albicans Chromosome Controlled study Diploidy Epidemiological monitoring Female Genetic variability Housekeeping gene Human Human experiment Major clinical study Molecular epidemiology Multilocus sequence typing Nonhuman Population genetic structure Vagina Validation process Candida albicans Molecular epidemiology Multilocus sequence typing Population genetic structure Recombination |
title_short |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
title_full |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
title_fullStr |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
title_full_unstemmed |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
title_sort |
Dissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing scheme |
dc.subject.keyword.spa.fl_str_mv |
Adult Article Bootstrapping Candida albicans Chromosome Controlled study Diploidy Epidemiological monitoring Female Genetic variability Housekeeping gene Human Human experiment Major clinical study Molecular epidemiology Multilocus sequence typing Nonhuman Population genetic structure Vagina Validation process Candida albicans Molecular epidemiology Multilocus sequence typing Population genetic structure Recombination |
topic |
Adult Article Bootstrapping Candida albicans Chromosome Controlled study Diploidy Epidemiological monitoring Female Genetic variability Housekeeping gene Human Human experiment Major clinical study Molecular epidemiology Multilocus sequence typing Nonhuman Population genetic structure Vagina Validation process Candida albicans Molecular epidemiology Multilocus sequence typing Population genetic structure Recombination |
description |
Candida albicans is a fungal opportunistic pathogen of significant public health importance mainly due to the recent emergence of strains with increased aggressiveness and antifungal resistance. Here, we aimed to describe the epidemiological profiles and approximate the population structure of C. albicans by analyzing the C. albicans multilocus sequence typing (MLST) database (Calb-MLST-DB), which contains the largest publically available dataset for this species. Based on 4,318 database isolates, we confirmed the ubiquitous nature of C. albicans including a group of diploid sequence types (DSTs) obtained from Healthy individuals exclusively (taken as an indicator of lack of association with illnesses in its host), until isolates established from Non-Healthy individuals (potentially associated with pathogenic processes) and other DSTs reported in both types (Healthy and Non-Healthy). The highest number of reported DSTs was related to blood, oral and vaginal swabs (32.4, 20.5, and 13.8%, respectively). High genetic diversity was observed in the seven housekeeping genes included in the MLST scheme, with a diverse population structure (154 clonal complexes, CCs; and a high number of singletons, n = 1,074). Phylogenetic reconstruction on the concatenated alignment of these housekeeping genes for all the reported DSTs (n = 3,483) was partially concordant with the CC assignment, however, an absence of bootstrap threshold supported nodes or p-distance, and the lack of association with the other epidemiological variables, evidenced the limitations of the MLST scheme. Marked genetic admixture signals were identified by STRUCTURE, with the majority being attributable to recombination events according to the RDP program results, although another type of exchange event cannot be ruled out. Our results reaffirm the genetic diversity inherent in the genes used for the MLST scheme, which are associated with the chromosomal remodeling already proposed for C. albicans. This was also corroborated with an internal validation at a micro geographical scale. Despite these results are biased due to the unavailability of considering the broad global spectrum of C. albicans isolates around the world. This suggests that the strategy used to population type this pathogen should be reevaluated to improve epidemiological monitoring of its health impact. Copyright © 2019 Muñoz, Wintaco, Muñoz and Ramírez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
publishDate |
2019 |
dc.date.created.spa.fl_str_mv |
2019 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:08:33Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:08:33Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3389/fmicb.2019.01052 |
dc.identifier.issn.none.fl_str_mv |
1664302X |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/24093 |
url |
https://doi.org/10.3389/fmicb.2019.01052 https://repository.urosario.edu.co/handle/10336/24093 |
identifier_str_mv |
1664302X |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. MAY |
dc.relation.citationTitle.none.fl_str_mv |
Frontiers in Microbiology |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 10 |
dc.relation.ispartof.spa.fl_str_mv |
Frontiers in Microbiology, ISSN:1664302X, Vol.10, No.MAY (2019) |
dc.relation.uri.spa.fl_str_mv |
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http://purl.org/coar/access_right/c_abf2 |
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Abierto (Texto Completo) |
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Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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Frontiers Media S.A. |
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Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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453006a5-2ec3-4faf-8e32-a9d7075d519d-1677b1acf-2e98-4b26-8633-3cbb71ad1e16-17cb03a25-9bd0-42b0-a596-4f422c2cfa37-110117161186002020-05-26T00:08:33Z2020-05-26T00:08:33Z2019Candida albicans is a fungal opportunistic pathogen of significant public health importance mainly due to the recent emergence of strains with increased aggressiveness and antifungal resistance. Here, we aimed to describe the epidemiological profiles and approximate the population structure of C. albicans by analyzing the C. albicans multilocus sequence typing (MLST) database (Calb-MLST-DB), which contains the largest publically available dataset for this species. Based on 4,318 database isolates, we confirmed the ubiquitous nature of C. albicans including a group of diploid sequence types (DSTs) obtained from Healthy individuals exclusively (taken as an indicator of lack of association with illnesses in its host), until isolates established from Non-Healthy individuals (potentially associated with pathogenic processes) and other DSTs reported in both types (Healthy and Non-Healthy). The highest number of reported DSTs was related to blood, oral and vaginal swabs (32.4, 20.5, and 13.8%, respectively). High genetic diversity was observed in the seven housekeeping genes included in the MLST scheme, with a diverse population structure (154 clonal complexes, CCs; and a high number of singletons, n = 1,074). Phylogenetic reconstruction on the concatenated alignment of these housekeeping genes for all the reported DSTs (n = 3,483) was partially concordant with the CC assignment, however, an absence of bootstrap threshold supported nodes or p-distance, and the lack of association with the other epidemiological variables, evidenced the limitations of the MLST scheme. Marked genetic admixture signals were identified by STRUCTURE, with the majority being attributable to recombination events according to the RDP program results, although another type of exchange event cannot be ruled out. Our results reaffirm the genetic diversity inherent in the genes used for the MLST scheme, which are associated with the chromosomal remodeling already proposed for C. albicans. This was also corroborated with an internal validation at a micro geographical scale. Despite these results are biased due to the unavailability of considering the broad global spectrum of C. albicans isolates around the world. This suggests that the strategy used to population type this pathogen should be reevaluated to improve epidemiological monitoring of its health impact. Copyright © 2019 Muñoz, Wintaco, Muñoz and Ramírez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.application/pdfhttps://doi.org/10.3389/fmicb.2019.010521664302Xhttps://repository.urosario.edu.co/handle/10336/24093engFrontiers Media S.A.No. MAYFrontiers in MicrobiologyVol. 10Frontiers in Microbiology, ISSN:1664302X, Vol.10, No.MAY (2019)https://www.scopus.com/inward/record.uri?eid=2-s2.0-85068621121&doi=10.3389%2ffmicb.2019.01052&partnerID=40&md5=ffc8f01414dec8836e01ebca2bc5a19bAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAdultArticleBootstrappingCandida albicansChromosomeControlled studyDiploidyEpidemiological monitoringFemaleGenetic variabilityHousekeeping geneHumanHuman experimentMajor clinical studyMolecular epidemiologyMultilocus sequence typingNonhumanPopulation genetic structureVaginaValidation processCandida albicansMolecular epidemiologyMultilocus sequence typingPopulation genetic structureRecombinationDissecting the Heterogeneous Population Genetic Structure of Candida albicans: Limitations and constraints of the multilocus sequence typing schemearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Muñoz, MarinaWintaco, Luz MairaMuñoz, Shirly AlexandraRamírez, Juan DavidORIGINALfmicb-10-01052.pdfapplication/pdf4054326https://repository.urosario.edu.co/bitstreams/26259894-9c50-4e96-b6e3-dbb06cbee611/download012ec9ebf695595c992d1993522f4f88MD51TEXTfmicb-10-01052.pdf.txtfmicb-10-01052.pdf.txtExtracted texttext/plain76622https://repository.urosario.edu.co/bitstreams/67a6593d-e64f-4d76-9ce9-995b6ea521a7/download890b0c5896e2ca476974c914cd386239MD52THUMBNAILfmicb-10-01052.pdf.jpgfmicb-10-01052.pdf.jpgGenerated Thumbnailimage/jpeg4321https://repository.urosario.edu.co/bitstreams/b3c6b389-6a46-4e03-a770-1a63f3180681/download8798a48abde9899dd121837f338daf18MD5310336/24093oai:repository.urosario.edu.co:10336/240932022-05-02 07:37:21.397308https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |