RNA-seq in kinetoplastids: A powerful tool for the understanding of the biology and host-pathogen interactions

The kinetoplastids include a large number of parasites responsible for serious diseases in humans and animals (Leishmania and Trypanosoma brucei) considered endemic in several regions of the world. These parasites are characterized by digenetic life cycles that undergo morphological and genetic changes that allow them to adapt to different microenvironments on their vertebrates and invertebrates hosts. Recent advances in ´omics´ technology, specifically transcriptomics have allowed to reveal aspects associated with such molecular changes. So far, different techniques have been used to evaluate the gene expression profile during the various stages of the life cycle of these parasites and during the host-parasite interactions. However, some of them have serious drawbacks that limit the precise study and full understanding of their transcriptomes. Therefore, recently has been implemented the latest technology (RNA-seq), which overcomes the drawbacks of traditional methods. In this review, studies that so far have used RNA-seq are presented and allowed to expand our knowledge regarding the biology of these parasites and their interactions with their hosts. © 2017 Elsevier B.V.

• Fecha de publicación: 2017
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/24303
• Acceso en línea: https://doi.org/10.1016/j.meegid.2017.02.003; https://repository.urosario.edu.co/handle/10336/24303
• Palabra clave: Messenger RNA; Ribosome RNA; Transcriptome; Protozoal RNA; Transcriptome; 3' untranslated region; 5' untranslated region; African trypanosomiasis; DNA replication; Gene duplication; Gene expression profiling; Host pathogen interaction; Human; In vitro study; Leishmania amazonensis; Leishmania donovani; Leishmania major; Leishmania mexicana; Life cycle stage; Measurement precision; Microarray analysis; Mucocutaneous leishmaniasis; Nonhuman; Polyadenylation; Review; RNA sequence; Single nucleotide polymorphism; Skin leishmaniasis; Transcriptomics; Trypanosoma brucei gambiense; Animal; Diptera; Gene expression regulation; Genetics; High throughput sequencing; Host parasite interaction; Insect vector; Leishmania; Macrophage; Parasitology; Trypanosoma brucei brucei; Animals; Diptera; Gene Expression Profiling; Gene Expression Regulation; High-Throughput Nucleotide Sequencing; Host-Parasite Interactions; Humans; Insect Vectors; Leishmania; Life Cycle Stages; Macrophages; Microarray Analysis; Transcriptome; Trypanosoma brucei brucei; Microarrays; RNA-seq; Transcriptome; Transcriptomics; Protozoan; development and aging; Growth; RNA
• License: Abierto (Texto Completo)