Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.

Several determining factors are involved in HPV infection outcomes; human leukocyte antigen (HLA) polymorphisms have been described as related factors. This study has ascertained the effect of genetic variation on HLA-DRB1 and DQB1 genes on HPV-16/-18/-31/-33/-45 and -58 clearance and redetection in...

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Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/25916
Acceso en línea:
https://doi.org/10.1038/s41598-020-64268-x
https://repository.urosario.edu.co/handle/10336/25916
Palabra clave:
Human papilloma virus (HPV)
Polymorphisms
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network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 4e6d439b-8bc4-4d08-9e81-1d9e2ba8fa22-1c67d367e-96c2-4566-8a39-374ead1a65f6-1fb1196f4-b5e5-4188-b69d-ec64c7dc41aa-169762621-b985-4e26-8d05-2637a79c5e2f-11cf1b50e-cf15-4152-9a42-54c731a835a4-15e48b055-035f-44d8-9867-a21686777f9d-1796530656002020-08-06T16:20:12Z2020-08-06T16:20:12Z2020Several determining factors are involved in HPV infection outcomes; human leukocyte antigen (HLA) polymorphisms have been described as related factors. This study has ascertained the effect of genetic variation on HLA-DRB1 and DQB1 genes on HPV-16/-18/-31/-33/-45 and -58 clearance and redetection in Colombian women. PCR and qPCR were used for viral identification and the Illumina MiSeq system was used for HLA-typing of cervical samples (n=276). Survival models were adjusted for identifying alleles/haplotypes related to HPV clearance/redetection; L1/L2 protein-epitope binding to MHC-II molecules was also predicted. Significant associations suggested effects favouring or hampering clearance/redetection events depending on the viral type involved in infection, e.g. just DRB1*12:01:01G favoured HPV-16 (coeff: 4.8) and HPV-45 clearance (coeff: 12.65) whilst HPV-18 (coeff: 2E-15), HPV-31 (coeff: 8E-17) and HPV-58 hindered elimination (coeff: 1E-14). An effect was only observed for some alelles when configured as haplotypes, e.g. DRB1*04:07:01G (having the greatest frequency in the target population) was associated with DQB1*02:01:1G or *03:02:03. Epitope prediction identified 23 clearance-related peptides and 29 were redetection-related; eight might have been related to HPV-16/-18 and -58 persistence and one to HPV-18 elimination. HLA allele/haplotype relationship with the course of HPV infection (clearance/redetection) depended on the infecting HPV type, in line with the specific viral epitopes displayed.application/pdfhttps://doi.org/10.1038/s41598-020-64268-xISSN: 2045-2322https://repository.urosario.edu.co/handle/10336/25916engNature Publishing Group7306No. 17306Scientific reportsVol. 10Scientific reports, ISSN:2045-2322, Vol.10, No.1 (2020); pp.7306-7306https://www.nature.com/articles/s41598-020-64268-x.pdfAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Scientific reportsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURHuman papilloma virus (HPV)PolymorphismsIdentifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.Identificar las moléculas HLA DRB1-DQB1 y predecir los epítopos asociados con la eliminación y redetección de la infección por VPH de alto riesgo.articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Del Rio-Ospina, LuisaCamargo, MilenaSoto-De Leon, Sara CSanchez, RicardoMoreno-Perez, Darwin APatarroyo, Manuel EPatarroyo, Manuel A.10336/25916oai:repository.urosario.edu.co:10336/259162022-05-02 07:37:15.224349https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
dc.title.TranslatedTitle.spa.fl_str_mv Identificar las moléculas HLA DRB1-DQB1 y predecir los epítopos asociados con la eliminación y redetección de la infección por VPH de alto riesgo.
title Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
spellingShingle Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
Human papilloma virus (HPV)
Polymorphisms
title_short Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
title_full Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
title_fullStr Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
title_full_unstemmed Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
title_sort Identifying the HLA DRB1-DQB1 molecules and predicting epitopes associated with high-risk HPV infection clearance and redetection.
dc.subject.keyword.spa.fl_str_mv Human papilloma virus (HPV)
Polymorphisms
topic Human papilloma virus (HPV)
Polymorphisms
description Several determining factors are involved in HPV infection outcomes; human leukocyte antigen (HLA) polymorphisms have been described as related factors. This study has ascertained the effect of genetic variation on HLA-DRB1 and DQB1 genes on HPV-16/-18/-31/-33/-45 and -58 clearance and redetection in Colombian women. PCR and qPCR were used for viral identification and the Illumina MiSeq system was used for HLA-typing of cervical samples (n=276). Survival models were adjusted for identifying alleles/haplotypes related to HPV clearance/redetection; L1/L2 protein-epitope binding to MHC-II molecules was also predicted. Significant associations suggested effects favouring or hampering clearance/redetection events depending on the viral type involved in infection, e.g. just DRB1*12:01:01G favoured HPV-16 (coeff: 4.8) and HPV-45 clearance (coeff: 12.65) whilst HPV-18 (coeff: 2E-15), HPV-31 (coeff: 8E-17) and HPV-58 hindered elimination (coeff: 1E-14). An effect was only observed for some alelles when configured as haplotypes, e.g. DRB1*04:07:01G (having the greatest frequency in the target population) was associated with DQB1*02:01:1G or *03:02:03. Epitope prediction identified 23 clearance-related peptides and 29 were redetection-related; eight might have been related to HPV-16/-18 and -58 persistence and one to HPV-18 elimination. HLA allele/haplotype relationship with the course of HPV infection (clearance/redetection) depended on the infecting HPV type, in line with the specific viral epitopes displayed.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:20:12Z
dc.date.available.none.fl_str_mv 2020-08-06T16:20:12Z
dc.date.created.spa.fl_str_mv 2020
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1038/s41598-020-64268-x
dc.identifier.issn.none.fl_str_mv ISSN: 2045-2322
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/25916
url https://doi.org/10.1038/s41598-020-64268-x
https://repository.urosario.edu.co/handle/10336/25916
identifier_str_mv ISSN: 2045-2322
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 7306
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 7306
dc.relation.citationTitle.none.fl_str_mv Scientific reports
dc.relation.citationVolume.none.fl_str_mv Vol. 10
dc.relation.ispartof.spa.fl_str_mv Scientific reports, ISSN:2045-2322, Vol.10, No.1 (2020); pp.7306-7306
dc.relation.uri.spa.fl_str_mv https://www.nature.com/articles/s41598-020-64268-x.pdf
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Nature Publishing Group
dc.source.spa.fl_str_mv Scientific reports
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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