Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto

La clase Clostridia está compuesta por bacterias con diversos roles potenciales, contando con especies comensales y otras patógenos oportunistas. Estas bacterias son clasificadas como anaerobias y están presentes a nivel intestinal de humanos y animales; sin embargo, la alta resistencia de sus espor...

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Fecha de publicación:
2024
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Universidad del Rosario
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Repositorio EdocUR - U. Rosario
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spa
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oai:repository.urosario.edu.co:10336/43372
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https://repository.urosario.edu.co/handle/10336/43372
Palabra clave:
Clostridiales
Patógenos
Marcadores de resistencia microbiana
Factores de Virulencia
Clostridials
Pathogens
Antimicrobial Resistance Markers
Virulence Factors
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id EDOCUR2_cef651979c612ac4557246ae835f8453
oai_identifier_str oai:repository.urosario.edu.co:10336/43372
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.none.fl_str_mv Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
dc.title.TranslatedTitle.none.fl_str_mv Genomic Characterization of Circulating Clostridial Species in the Pasto River
title Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
spellingShingle Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
Clostridiales
Patógenos
Marcadores de resistencia microbiana
Factores de Virulencia
Clostridials
Pathogens
Antimicrobial Resistance Markers
Virulence Factors
title_short Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
title_full Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
title_fullStr Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
title_full_unstemmed Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
title_sort Caracterización genómica de especies de Clostridiales Circulantes en el río Pasto
dc.contributor.advisor.none.fl_str_mv Muñoz Díaz, Marina
dc.contributor.gruplac.none.fl_str_mv Grupo de Investigaciones Microbiológicas UR (GIMUR)
dc.subject.none.fl_str_mv Clostridiales
Patógenos
Marcadores de resistencia microbiana
Factores de Virulencia
topic Clostridiales
Patógenos
Marcadores de resistencia microbiana
Factores de Virulencia
Clostridials
Pathogens
Antimicrobial Resistance Markers
Virulence Factors
dc.subject.keyword.none.fl_str_mv Clostridials
Pathogens
Antimicrobial Resistance Markers
Virulence Factors
description La clase Clostridia está compuesta por bacterias con diversos roles potenciales, contando con especies comensales y otras patógenos oportunistas. Estas bacterias son clasificadas como anaerobias y están presentes a nivel intestinal de humanos y animales; sin embargo, la alta resistencia de sus esporas al oxígeno les permite ampliar su rango de dispersión, siendo encontrados en muestras ambientales de suelos y aguas. La presencia de este tipo de microorganismos en cuerpos de agua puede ser un marcador de pérdida de su calidad. Zonas en las que es frecuente el contacto entre poblaciones humanas y cuerpos hídricos pueden ser propensas a contaminación de diferentes tipos, siendo una de ellas los microorganismos, muchos de los cuales son de importancia clínica por su potencial patógeno. Este estudio tuvo como objetivo detectar y caracterizar a nivel genómico bacterias Clostridiales aisladas de muestras de agua, tomadas entre septiembre del 2022 hasta marzo del 2023 del Río Pasto. Para el proceso de caracterización se ensamblaron Genomas Metagenómicos Ensamblados (MAGs) en los cuales se identificaron marcadores de resistencia a antimicrobianos y factores de virulencia, siendo esto tema relevante de salud pública. Se ensamblaron 33 MAGs de alta calidad y cuatro de calidad media, correspondientes a 11 especies clostridiales, estando entre estas Clostridium perfringens, Clostridium tetani, Clostridium botulinum y Clostridioides difficile, que son medicamente relevantes. Pymaiobacter missiliensis y Clostridium sulfidigenes también fueron detectadas en este estudio, siendo especies Clostridiales recientemente descritas y poco estudiadas, por lo que no es claro su impacto sobre hospederos. La búsqueda de factores de virulencia a partir de los MAGs mostró la presencia de genes nag, junto con otros genes de este tipo frecuentes en C. perfringens. Adicionalmente, se encontraron marcadores de resistencia a antibióticos, especialmente asociados a resistencia a fluoroquinolonas, aminoglucósidos, lincosamidas, péptidos y tetraciclinas, ampliamente distribuidos en los MAGs obtenidos. En este estudio se identificó la presencia de 11 especies Clostridiales en cuerpos de agua, las cuales transportan factores de virulencia y marcadores de resistencia, revelando un riesgo microbiológico en el río Pasto.
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-09-03T20:58:06Z
dc.date.available.none.fl_str_mv 2024-09-03T20:58:06Z
dc.date.created.none.fl_str_mv 2024-08-22
dc.type.none.fl_str_mv bachelorThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.document.none.fl_str_mv Trabajo de grado
dc.type.spa.none.fl_str_mv Trabajo de grado
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/43372
url https://repository.urosario.edu.co/handle/10336/43372
dc.language.iso.none.fl_str_mv spa
language spa
dc.rights.*.fl_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.none.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
rights_invalid_str_mv Attribution-NonCommercial-ShareAlike 4.0 International
Abierto (Texto Completo)
http://creativecommons.org/licenses/by-nc-sa/4.0/
http://purl.org/coar/access_right/c_abf2
dc.format.extent.none.fl_str_mv 34 pp
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad del Rosario
dc.publisher.department.none.fl_str_mv Facultad de Ciencias Naturales
dc.publisher.program.none.fl_str_mv Biología
publisher.none.fl_str_mv Universidad del Rosario
institution Universidad del Rosario
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spelling Muñoz Díaz, Marina11e33b66-c06e-405c-9a7d-cbb5b723fd32-1Grupo de Investigaciones Microbiológicas UR (GIMUR)Fernández Sánchez, Juan DiegoBiólogoPregradoFull timeb92085b3-b7eb-4485-8950-35e6d2351696-12024-09-03T20:58:06Z2024-09-03T20:58:06Z2024-08-22La clase Clostridia está compuesta por bacterias con diversos roles potenciales, contando con especies comensales y otras patógenos oportunistas. Estas bacterias son clasificadas como anaerobias y están presentes a nivel intestinal de humanos y animales; sin embargo, la alta resistencia de sus esporas al oxígeno les permite ampliar su rango de dispersión, siendo encontrados en muestras ambientales de suelos y aguas. La presencia de este tipo de microorganismos en cuerpos de agua puede ser un marcador de pérdida de su calidad. Zonas en las que es frecuente el contacto entre poblaciones humanas y cuerpos hídricos pueden ser propensas a contaminación de diferentes tipos, siendo una de ellas los microorganismos, muchos de los cuales son de importancia clínica por su potencial patógeno. Este estudio tuvo como objetivo detectar y caracterizar a nivel genómico bacterias Clostridiales aisladas de muestras de agua, tomadas entre septiembre del 2022 hasta marzo del 2023 del Río Pasto. Para el proceso de caracterización se ensamblaron Genomas Metagenómicos Ensamblados (MAGs) en los cuales se identificaron marcadores de resistencia a antimicrobianos y factores de virulencia, siendo esto tema relevante de salud pública. Se ensamblaron 33 MAGs de alta calidad y cuatro de calidad media, correspondientes a 11 especies clostridiales, estando entre estas Clostridium perfringens, Clostridium tetani, Clostridium botulinum y Clostridioides difficile, que son medicamente relevantes. Pymaiobacter missiliensis y Clostridium sulfidigenes también fueron detectadas en este estudio, siendo especies Clostridiales recientemente descritas y poco estudiadas, por lo que no es claro su impacto sobre hospederos. La búsqueda de factores de virulencia a partir de los MAGs mostró la presencia de genes nag, junto con otros genes de este tipo frecuentes en C. perfringens. Adicionalmente, se encontraron marcadores de resistencia a antibióticos, especialmente asociados a resistencia a fluoroquinolonas, aminoglucósidos, lincosamidas, péptidos y tetraciclinas, ampliamente distribuidos en los MAGs obtenidos. En este estudio se identificó la presencia de 11 especies Clostridiales en cuerpos de agua, las cuales transportan factores de virulencia y marcadores de resistencia, revelando un riesgo microbiológico en el río Pasto.The Clostridia class comprises bacteria with diverse roles, including commensal species and opportunistic pathogens. These bacteria are classified as anaerobic and are present in the gut of animals, including humans. Their spores are highly oxygen resistant, allowing them to expand their distribution in environmental samples such as soil and water. The presence of these bacteria in water bodies is an indicator of low quality, and areas of frequent contact between humans and water bodies are prone to multiple sources of contamination, including microorganisms. In particular, the presence of Clostridia in water is clinically relevant due to its pathogenic potential. This study aimed to detect Clostridial bacteria from water samples collected in the Pasto River between September 2022 and March 2023 and characterize them at the genomic level. We assembled Metagenome-Assembled Genomes (MAGs) in which we identified antimicrobial resistance markers and virulence factors, making this a relevant public health study. A total of 33 high-quality and four medium-quality MAGs were assembled, corresponding to 11 species of these bacteria, including Clostridium perfringens, Clostridium tetani, Clostridium botulinum, and Clostridioides difficile, which are medically relevant clostridial species. Notably, we detected the presence of the rarely reported species Pymaiobacter missiliensis and Clostridium sulfidigenes, both recently described Clostridial species that are poorly studied and for which there are no reported infections. Among the virulence factors identified were nag and related genes frequently found in strain 13 of C. perfringens. We also detected antibiotic-resistance markers associated with fluoroquinolones, aminoglycosides, lincosamides, peptides, and tetracyclines in the MAGs assembled. This study identified 11 Clostridial species in water bodies, which carry virulence factors and resistance markers, revealing a microbiological risk in the Pasto River.Facultad de Ciencias Naturales de la Universidad del RosarioSistema General de Regalías34 ppapplication/pdfhttps://repository.urosario.edu.co/handle/10336/43372spaUniversidad del RosarioFacultad de Ciencias NaturalesBiologíaAttribution-NonCommercial-ShareAlike 4.0 InternationalAbierto (Texto Completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.http://creativecommons.org/licenses/by-nc-sa/4.0/http://purl.org/coar/access_right/c_abf2Adams, J. J., Gregg, K., Bayer, E. A., Boraston, A. B., & Smith, S. P. (2008). Structural basis of Clostridium perfringens toxin complex formation. 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