Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization

Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p...

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Autores:
Tipo de recurso:
Fecha de publicación:
2014
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24057
Acceso en línea:
https://doi.org/10.1111/1567-1364.12099
https://repository.urosario.edu.co/handle/10336/24057
Palabra clave:
Complementary RNA
Gamma interferon
HLA DR antigen
Oligonucleotide
Protein p27
Epitope
Fungal protein
Gamma interferon
HLA DR antigen
Peptide
Protein binding
Agrobacterium tumefaciens
Allele
Amino acid sequence
Article
Cell structure
Cell viability
Fungal gene
Fungal virulence
Fungus growth
Gene expression
Gene frequency
Macrophage
Mycelium
Nonhuman
Nucleotide sequence
Paracoccidioides brasiliensis
Pbp27 gene
Protein function
Real time polymerase chain reaction
T lymphocyte
Yeast cell
Chemistry
Drug effects
Epitope mapping
Gene expression regulation
Gene silencing
Genetics
Immunology
Metabolism
Microbiology
Paracoccidioides
Agrobacterium tumefaciens
Fungi
Paracoccidioides brasiliensis
Amino Acid Sequence
Epitope Mapping
Fungal Proteins
Gene Knockdown Techniques
HLA-DR Antigens
Interferon-gamma
Macrophages
Paracoccidioides
Peptides
Protein Binding
Antisense RNA
Fungal survival
Gene silencing
P27
Paracoccidioides brasiliensis
Pbp27
Fungal
T-Lymphocyte
Epitopes
Gene Expression Regulation
Rights
License
Abierto (Texto Completo)
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network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 6b0781e0-7736-4d42-b87a-6226d484858f-14b14aa4d-fc53-4f57-8712-bef93e9fcfa2-1d3033aaa-247e-4a6b-a1ac-d36d7fc69ef7-1b74e8c80-eb47-4f61-ab78-386e545d9143-1e8720667-0271-460d-919a-8a22de282cd7-11598a9c7-daee-42c6-83bf-7ddb0e350ad6-15a9ddfa9-4c89-466e-8675-4d15387b76ce-1b5474c71-8021-4539-8fc9-044da89e0012-12020-05-26T00:08:07Z2020-05-26T00:08:07Z2014Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. Therefore, in this study, through the use of antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 mutants (PbP27 aRNA) with the goal to evaluate the role of p27 in the biology and virulence of this fungus. PbP27 expression was reduced 60-75% in mutants, as determined by real-time PCR in correlation with a decrease in p27 expression. No alterations in the growth curve or in the ability to shift from mycelia to yeast or from yeast to mycelia were observed in PbP27 aRNA strains; however, we did observe a reduction in cell vitality. Moreover, a decrease in cell viability of PbP27 aRNA yeast cells after interaction with IFN-?-stimulated macrophages was detected. Based on these results, we propose that p27 plays a role in yeast cell architecture and represents one of the mechanisms employed by this fungus for its interaction with the monocyte/macrophage system. p27 plays a role in Paracoccidioides brasiliensis yeast cell architecture and represents one of the mechanisms employed by the fungus for its interaction with the monocyte/macrophage system. © 2013 Federation of European Microbiological Societies. Published by John Wiley and Sons Ltd. All rights reserved.application/pdfhttps://doi.org/10.1111/1567-1364.120991567136415671356https://repository.urosario.edu.co/handle/10336/24057engBlackwell Publishing Ltd280No. 2270FEMS Yeast ResearchVol. 14FEMS Yeast Research, ISSN:15671364, 15671356, Vol.14, No.2 (2014); pp. 270-280https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896111561&doi=10.1111%2f1567-1364.12099&partnerID=40&md5=fa0a735b09665d6bd9c7a3b09637e377Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementary RNAGamma interferonHLA DR antigenOligonucleotideProtein p27EpitopeFungal proteinGamma interferonHLA DR antigenPeptideProtein bindingAgrobacterium tumefaciensAlleleAmino acid sequenceArticleCell structureCell viabilityFungal geneFungal virulenceFungus growthGene expressionGene frequencyMacrophageMyceliumNonhumanNucleotide sequenceParacoccidioides brasiliensisPbp27 geneProtein functionReal time polymerase chain reactionT lymphocyteYeast cellChemistryDrug effectsEpitope mappingGene expression regulationGene silencingGeneticsImmunologyMetabolismMicrobiologyParacoccidioidesAgrobacterium tumefaciensFungiParacoccidioides brasiliensisAmino Acid SequenceEpitope MappingFungal ProteinsGene Knockdown TechniquesHLA-DR AntigensInterferon-gammaMacrophagesParacoccidioidesPeptidesProtein BindingAntisense RNAFungal survivalGene silencingP27Paracoccidioides brasiliensisPbp27FungalT-LymphocyteEpitopesGene Expression RegulationParacoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterizationarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Torres, IsauraHernandez, OrvilleTamayo, DianaMuñoz, Jose F.García, Ana M.Gómez, Beatriz L.Restrepo, AngelaMcEwen, Juan G.10336/24057oai:repository.urosario.edu.co:10336/240572022-05-02 07:37:21.38055https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
title Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
spellingShingle Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
Complementary RNA
Gamma interferon
HLA DR antigen
Oligonucleotide
Protein p27
Epitope
Fungal protein
Gamma interferon
HLA DR antigen
Peptide
Protein binding
Agrobacterium tumefaciens
Allele
Amino acid sequence
Article
Cell structure
Cell viability
Fungal gene
Fungal virulence
Fungus growth
Gene expression
Gene frequency
Macrophage
Mycelium
Nonhuman
Nucleotide sequence
Paracoccidioides brasiliensis
Pbp27 gene
Protein function
Real time polymerase chain reaction
T lymphocyte
Yeast cell
Chemistry
Drug effects
Epitope mapping
Gene expression regulation
Gene silencing
Genetics
Immunology
Metabolism
Microbiology
Paracoccidioides
Agrobacterium tumefaciens
Fungi
Paracoccidioides brasiliensis
Amino Acid Sequence
Epitope Mapping
Fungal Proteins
Gene Knockdown Techniques
HLA-DR Antigens
Interferon-gamma
Macrophages
Paracoccidioides
Peptides
Protein Binding
Antisense RNA
Fungal survival
Gene silencing
P27
Paracoccidioides brasiliensis
Pbp27
Fungal
T-Lymphocyte
Epitopes
Gene Expression Regulation
title_short Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
title_full Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
title_fullStr Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
title_full_unstemmed Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
title_sort Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
dc.subject.keyword.spa.fl_str_mv Complementary RNA
Gamma interferon
HLA DR antigen
Oligonucleotide
Protein p27
Epitope
Fungal protein
Gamma interferon
HLA DR antigen
Peptide
Protein binding
Agrobacterium tumefaciens
Allele
Amino acid sequence
Article
Cell structure
Cell viability
Fungal gene
Fungal virulence
Fungus growth
Gene expression
Gene frequency
Macrophage
Mycelium
Nonhuman
Nucleotide sequence
Paracoccidioides brasiliensis
Pbp27 gene
Protein function
Real time polymerase chain reaction
T lymphocyte
Yeast cell
Chemistry
Drug effects
Epitope mapping
Gene expression regulation
Gene silencing
Genetics
Immunology
Metabolism
Microbiology
Paracoccidioides
Agrobacterium tumefaciens
Fungi
Paracoccidioides brasiliensis
Amino Acid Sequence
Epitope Mapping
Fungal Proteins
Gene Knockdown Techniques
HLA-DR Antigens
Interferon-gamma
Macrophages
Paracoccidioides
Peptides
Protein Binding
Antisense RNA
Fungal survival
Gene silencing
P27
Paracoccidioides brasiliensis
Pbp27
topic Complementary RNA
Gamma interferon
HLA DR antigen
Oligonucleotide
Protein p27
Epitope
Fungal protein
Gamma interferon
HLA DR antigen
Peptide
Protein binding
Agrobacterium tumefaciens
Allele
Amino acid sequence
Article
Cell structure
Cell viability
Fungal gene
Fungal virulence
Fungus growth
Gene expression
Gene frequency
Macrophage
Mycelium
Nonhuman
Nucleotide sequence
Paracoccidioides brasiliensis
Pbp27 gene
Protein function
Real time polymerase chain reaction
T lymphocyte
Yeast cell
Chemistry
Drug effects
Epitope mapping
Gene expression regulation
Gene silencing
Genetics
Immunology
Metabolism
Microbiology
Paracoccidioides
Agrobacterium tumefaciens
Fungi
Paracoccidioides brasiliensis
Amino Acid Sequence
Epitope Mapping
Fungal Proteins
Gene Knockdown Techniques
HLA-DR Antigens
Interferon-gamma
Macrophages
Paracoccidioides
Peptides
Protein Binding
Antisense RNA
Fungal survival
Gene silencing
P27
Paracoccidioides brasiliensis
Pbp27
Fungal
T-Lymphocyte
Epitopes
Gene Expression Regulation
dc.subject.keyword.eng.fl_str_mv Fungal
T-Lymphocyte
Epitopes
Gene Expression Regulation
description Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. Therefore, in this study, through the use of antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 mutants (PbP27 aRNA) with the goal to evaluate the role of p27 in the biology and virulence of this fungus. PbP27 expression was reduced 60-75% in mutants, as determined by real-time PCR in correlation with a decrease in p27 expression. No alterations in the growth curve or in the ability to shift from mycelia to yeast or from yeast to mycelia were observed in PbP27 aRNA strains; however, we did observe a reduction in cell vitality. Moreover, a decrease in cell viability of PbP27 aRNA yeast cells after interaction with IFN-?-stimulated macrophages was detected. Based on these results, we propose that p27 plays a role in yeast cell architecture and represents one of the mechanisms employed by this fungus for its interaction with the monocyte/macrophage system. p27 plays a role in Paracoccidioides brasiliensis yeast cell architecture and represents one of the mechanisms employed by the fungus for its interaction with the monocyte/macrophage system. © 2013 Federation of European Microbiological Societies. Published by John Wiley and Sons Ltd. All rights reserved.
publishDate 2014
dc.date.created.spa.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:08:07Z
dc.date.available.none.fl_str_mv 2020-05-26T00:08:07Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1111/1567-1364.12099
dc.identifier.issn.none.fl_str_mv 15671364
15671356
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24057
url https://doi.org/10.1111/1567-1364.12099
https://repository.urosario.edu.co/handle/10336/24057
identifier_str_mv 15671364
15671356
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 280
dc.relation.citationIssue.none.fl_str_mv No. 2
dc.relation.citationStartPage.none.fl_str_mv 270
dc.relation.citationTitle.none.fl_str_mv FEMS Yeast Research
dc.relation.citationVolume.none.fl_str_mv Vol. 14
dc.relation.ispartof.spa.fl_str_mv FEMS Yeast Research, ISSN:15671364, 15671356, Vol.14, No.2 (2014); pp. 270-280
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896111561&doi=10.1111%2f1567-1364.12099&partnerID=40&md5=fa0a735b09665d6bd9c7a3b09637e377
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Blackwell Publishing Ltd
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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