Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization
Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2014
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/24057
- Acceso en línea:
- https://doi.org/10.1111/1567-1364.12099
https://repository.urosario.edu.co/handle/10336/24057
- Palabra clave:
- Complementary RNA
Gamma interferon
HLA DR antigen
Oligonucleotide
Protein p27
Epitope
Fungal protein
Gamma interferon
HLA DR antigen
Peptide
Protein binding
Agrobacterium tumefaciens
Allele
Amino acid sequence
Article
Cell structure
Cell viability
Fungal gene
Fungal virulence
Fungus growth
Gene expression
Gene frequency
Macrophage
Mycelium
Nonhuman
Nucleotide sequence
Paracoccidioides brasiliensis
Pbp27 gene
Protein function
Real time polymerase chain reaction
T lymphocyte
Yeast cell
Chemistry
Drug effects
Epitope mapping
Gene expression regulation
Gene silencing
Genetics
Immunology
Metabolism
Microbiology
Paracoccidioides
Agrobacterium tumefaciens
Fungi
Paracoccidioides brasiliensis
Amino Acid Sequence
Epitope Mapping
Fungal Proteins
Gene Knockdown Techniques
HLA-DR Antigens
Interferon-gamma
Macrophages
Paracoccidioides
Peptides
Protein Binding
Antisense RNA
Fungal survival
Gene silencing
P27
Paracoccidioides brasiliensis
Pbp27
Fungal
T-Lymphocyte
Epitopes
Gene Expression Regulation
- Rights
- License
- Abierto (Texto Completo)
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6b0781e0-7736-4d42-b87a-6226d484858f-14b14aa4d-fc53-4f57-8712-bef93e9fcfa2-1d3033aaa-247e-4a6b-a1ac-d36d7fc69ef7-1b74e8c80-eb47-4f61-ab78-386e545d9143-1e8720667-0271-460d-919a-8a22de282cd7-11598a9c7-daee-42c6-83bf-7ddb0e350ad6-15a9ddfa9-4c89-466e-8675-4d15387b76ce-1b5474c71-8021-4539-8fc9-044da89e0012-12020-05-26T00:08:07Z2020-05-26T00:08:07Z2014Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. Therefore, in this study, through the use of antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 mutants (PbP27 aRNA) with the goal to evaluate the role of p27 in the biology and virulence of this fungus. PbP27 expression was reduced 60-75% in mutants, as determined by real-time PCR in correlation with a decrease in p27 expression. No alterations in the growth curve or in the ability to shift from mycelia to yeast or from yeast to mycelia were observed in PbP27 aRNA strains; however, we did observe a reduction in cell vitality. Moreover, a decrease in cell viability of PbP27 aRNA yeast cells after interaction with IFN-?-stimulated macrophages was detected. Based on these results, we propose that p27 plays a role in yeast cell architecture and represents one of the mechanisms employed by this fungus for its interaction with the monocyte/macrophage system. p27 plays a role in Paracoccidioides brasiliensis yeast cell architecture and represents one of the mechanisms employed by the fungus for its interaction with the monocyte/macrophage system. © 2013 Federation of European Microbiological Societies. Published by John Wiley and Sons Ltd. All rights reserved.application/pdfhttps://doi.org/10.1111/1567-1364.120991567136415671356https://repository.urosario.edu.co/handle/10336/24057engBlackwell Publishing Ltd280No. 2270FEMS Yeast ResearchVol. 14FEMS Yeast Research, ISSN:15671364, 15671356, Vol.14, No.2 (2014); pp. 270-280https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896111561&doi=10.1111%2f1567-1364.12099&partnerID=40&md5=fa0a735b09665d6bd9c7a3b09637e377Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementary RNAGamma interferonHLA DR antigenOligonucleotideProtein p27EpitopeFungal proteinGamma interferonHLA DR antigenPeptideProtein bindingAgrobacterium tumefaciensAlleleAmino acid sequenceArticleCell structureCell viabilityFungal geneFungal virulenceFungus growthGene expressionGene frequencyMacrophageMyceliumNonhumanNucleotide sequenceParacoccidioides brasiliensisPbp27 geneProtein functionReal time polymerase chain reactionT lymphocyteYeast cellChemistryDrug effectsEpitope mappingGene expression regulationGene silencingGeneticsImmunologyMetabolismMicrobiologyParacoccidioidesAgrobacterium tumefaciensFungiParacoccidioides brasiliensisAmino Acid SequenceEpitope MappingFungal ProteinsGene Knockdown TechniquesHLA-DR AntigensInterferon-gammaMacrophagesParacoccidioidesPeptidesProtein BindingAntisense RNAFungal survivalGene silencingP27Paracoccidioides brasiliensisPbp27FungalT-LymphocyteEpitopesGene Expression RegulationParacoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterizationarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Torres, IsauraHernandez, OrvilleTamayo, DianaMuñoz, Jose F.García, Ana M.Gómez, Beatriz L.Restrepo, AngelaMcEwen, Juan G.10336/24057oai:repository.urosario.edu.co:10336/240572022-05-02 07:37:21.38055https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
title |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
spellingShingle |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization Complementary RNA Gamma interferon HLA DR antigen Oligonucleotide Protein p27 Epitope Fungal protein Gamma interferon HLA DR antigen Peptide Protein binding Agrobacterium tumefaciens Allele Amino acid sequence Article Cell structure Cell viability Fungal gene Fungal virulence Fungus growth Gene expression Gene frequency Macrophage Mycelium Nonhuman Nucleotide sequence Paracoccidioides brasiliensis Pbp27 gene Protein function Real time polymerase chain reaction T lymphocyte Yeast cell Chemistry Drug effects Epitope mapping Gene expression regulation Gene silencing Genetics Immunology Metabolism Microbiology Paracoccidioides Agrobacterium tumefaciens Fungi Paracoccidioides brasiliensis Amino Acid Sequence Epitope Mapping Fungal Proteins Gene Knockdown Techniques HLA-DR Antigens Interferon-gamma Macrophages Paracoccidioides Peptides Protein Binding Antisense RNA Fungal survival Gene silencing P27 Paracoccidioides brasiliensis Pbp27 Fungal T-Lymphocyte Epitopes Gene Expression Regulation |
title_short |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
title_full |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
title_fullStr |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
title_full_unstemmed |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
title_sort |
Paracoccidioides brasiliensis PbP27 gene: Knockdown procedures and functional characterization |
dc.subject.keyword.spa.fl_str_mv |
Complementary RNA Gamma interferon HLA DR antigen Oligonucleotide Protein p27 Epitope Fungal protein Gamma interferon HLA DR antigen Peptide Protein binding Agrobacterium tumefaciens Allele Amino acid sequence Article Cell structure Cell viability Fungal gene Fungal virulence Fungus growth Gene expression Gene frequency Macrophage Mycelium Nonhuman Nucleotide sequence Paracoccidioides brasiliensis Pbp27 gene Protein function Real time polymerase chain reaction T lymphocyte Yeast cell Chemistry Drug effects Epitope mapping Gene expression regulation Gene silencing Genetics Immunology Metabolism Microbiology Paracoccidioides Agrobacterium tumefaciens Fungi Paracoccidioides brasiliensis Amino Acid Sequence Epitope Mapping Fungal Proteins Gene Knockdown Techniques HLA-DR Antigens Interferon-gamma Macrophages Paracoccidioides Peptides Protein Binding Antisense RNA Fungal survival Gene silencing P27 Paracoccidioides brasiliensis Pbp27 |
topic |
Complementary RNA Gamma interferon HLA DR antigen Oligonucleotide Protein p27 Epitope Fungal protein Gamma interferon HLA DR antigen Peptide Protein binding Agrobacterium tumefaciens Allele Amino acid sequence Article Cell structure Cell viability Fungal gene Fungal virulence Fungus growth Gene expression Gene frequency Macrophage Mycelium Nonhuman Nucleotide sequence Paracoccidioides brasiliensis Pbp27 gene Protein function Real time polymerase chain reaction T lymphocyte Yeast cell Chemistry Drug effects Epitope mapping Gene expression regulation Gene silencing Genetics Immunology Metabolism Microbiology Paracoccidioides Agrobacterium tumefaciens Fungi Paracoccidioides brasiliensis Amino Acid Sequence Epitope Mapping Fungal Proteins Gene Knockdown Techniques HLA-DR Antigens Interferon-gamma Macrophages Paracoccidioides Peptides Protein Binding Antisense RNA Fungal survival Gene silencing P27 Paracoccidioides brasiliensis Pbp27 Fungal T-Lymphocyte Epitopes Gene Expression Regulation |
dc.subject.keyword.eng.fl_str_mv |
Fungal T-Lymphocyte Epitopes Gene Expression Regulation |
description |
Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. Therefore, in this study, through the use of antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 mutants (PbP27 aRNA) with the goal to evaluate the role of p27 in the biology and virulence of this fungus. PbP27 expression was reduced 60-75% in mutants, as determined by real-time PCR in correlation with a decrease in p27 expression. No alterations in the growth curve or in the ability to shift from mycelia to yeast or from yeast to mycelia were observed in PbP27 aRNA strains; however, we did observe a reduction in cell vitality. Moreover, a decrease in cell viability of PbP27 aRNA yeast cells after interaction with IFN-?-stimulated macrophages was detected. Based on these results, we propose that p27 plays a role in yeast cell architecture and represents one of the mechanisms employed by this fungus for its interaction with the monocyte/macrophage system. p27 plays a role in Paracoccidioides brasiliensis yeast cell architecture and represents one of the mechanisms employed by the fungus for its interaction with the monocyte/macrophage system. © 2013 Federation of European Microbiological Societies. Published by John Wiley and Sons Ltd. All rights reserved. |
publishDate |
2014 |
dc.date.created.spa.fl_str_mv |
2014 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:08:07Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:08:07Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1111/1567-1364.12099 |
dc.identifier.issn.none.fl_str_mv |
15671364 15671356 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/24057 |
url |
https://doi.org/10.1111/1567-1364.12099 https://repository.urosario.edu.co/handle/10336/24057 |
identifier_str_mv |
15671364 15671356 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
280 |
dc.relation.citationIssue.none.fl_str_mv |
No. 2 |
dc.relation.citationStartPage.none.fl_str_mv |
270 |
dc.relation.citationTitle.none.fl_str_mv |
FEMS Yeast Research |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 14 |
dc.relation.ispartof.spa.fl_str_mv |
FEMS Yeast Research, ISSN:15671364, 15671356, Vol.14, No.2 (2014); pp. 270-280 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84896111561&doi=10.1111%2f1567-1364.12099&partnerID=40&md5=fa0a735b09665d6bd9c7a3b09637e377 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Blackwell Publishing Ltd |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167732638187520 |