Agreement of the Kato-Katz test established by the WHO with samples fixed with sodium acetate analyzed at 6 months to diagnose intestinal geohelminthes

The aim of this study was to evaluate the performance of the Kato-Katz test (WHO version) with stool samples from a rural area, fixed with sodium acetate (SAF). The Kato-Katz test was used to compare unfixed samples (conventional test) with the same samples containing SAF fixative at time 0 and at 6 months. The study included stools from 154 subjects.A marginally statistically significant decrease in prevalence was estimated only for hookworm, when comparing unfixed samples versus the SAF fixed samples read at 6 months (. p=. 0.06). A significant reduction in parasite load was found for hookworm (. p less than . 0.01) and Trichuris trichiura (. p less than . 0.01) between the unfixed and the fixed sample read at 6 months, but not for Ascaris lumbricoides (. p=. 0.10). This research suggests that the SAF fixative solution is a good option for transporting samples for diagnosis, especially in rural areas in developing countries. © 2015 Elsevier B.V.

• Fecha de publicación: 2015
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23619
• Acceso en línea: https://doi.org/10.1016/j.actatropica.2015.03.004; https://repository.urosario.edu.co/handle/10336/23619
• Palabra clave: Acetic acid; Fixative; Acetic acid; Soil; Acetate; Developing world; Endoparasite; Fecal pellet; Public health; Sodium; Soilborne disease; World health organization; Article; Ascaris lumbricoides; Controlled study; Diagnostic test accuracy study; Feces analysis; Helminthiasis; Hookworm; Intestinal geohelminthes; Intestine infection; Kato katz test; Microbiological examination; Nonhuman; Parasite identification; Parasite load; Prevalence; Rural area; Trichuris trichiura; World health organization; Adult; Animal; Ascariasis; Colombia; Comparative study; Developing country; Feces; Female; Helminthiasis; Hookworm infections; Human; Intestine; Isolation and purification; Male; Middle aged; Parasite identification; Parasitology; Sensitivity and specificity; Soil; Time; Transport at the cellular level; Trichuris; Colombia; Ancylostomatoidea; Ascaris; Ascaris lumbricoides; Trichuris trichiura; Vermes; Adult; Ancylostomatoidea; Animals; Ascariasis; Ascaris lumbricoides; Biological transport; Colombia; Developing countries; Feces; Female; Fixatives; Helminthiasis; Hookworm infections; Humans; Intestines; Male; Middle aged; Parasite egg count; Parasite load; Prevalence; Sensitivity and specificity; Sodium acetate; Soil; Time factors; Trichuris; World health organization; Ascaris; Colombia; Diagnosis; Hookworm; Soil-transmitted helminth; parasitic; parasitic; Intestinal diseases; Intestinal diseases
• License: Abierto (Texto Completo)