Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7

El glioblastoma multiforme (GBM) es uno de los tipos de cáncer con mayor letalidad. El promedio de vida para todos los pacientes es de 12-18 meses después del diagnóstico y tratamiento. Los principales tratamientos para combatir el glioblastoma son: la cirugía y radioterapia. La cirugía principalmen...

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Fecha de publicación:
2021
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/31625
Acceso en línea:
https://doi.org/10.48713/10336_31625
https://repository.urosario.edu.co/handle/10336/31625
Palabra clave:
Glioblastoma
Puntos de carbono
Citotoxicidad
Azul tripán
Ingeniería & operaciones afines
Ciencias médicas - Innovaciones tecnológicas
Bioingeniería
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License
Atribución-NoComercial-SinDerivadas 2.5 Colombia
id EDOCUR2_bd2bfec86aa6430f0a3c1fa6003ceefc
oai_identifier_str oai:repository.urosario.edu.co:10336/31625
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
dc.title.TranslatedTitle.spa.fl_str_mv Evaluation of carbon point (CD) cytotoxicity in tumor cell lines U-87 and MCF-7
title Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
spellingShingle Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
Glioblastoma
Puntos de carbono
Citotoxicidad
Azul tripán
Ingeniería & operaciones afines
Ciencias médicas - Innovaciones tecnológicas
Bioingeniería
title_short Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
title_full Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
title_fullStr Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
title_full_unstemmed Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
title_sort Evaluación de la citotoxicidad de puntos de carbono (CD) en las líneas celulares tumorales U-87 Y MCF-7
dc.contributor.advisor.none.fl_str_mv Rodríguez Burbano, Diana Consuelo
Ondo Méndez, Alejandro Oyono
dc.subject.spa.fl_str_mv Glioblastoma
Puntos de carbono
Citotoxicidad
Azul tripán
topic Glioblastoma
Puntos de carbono
Citotoxicidad
Azul tripán
Ingeniería & operaciones afines
Ciencias médicas - Innovaciones tecnológicas
Bioingeniería
dc.subject.ddc.spa.fl_str_mv Ingeniería & operaciones afines
dc.subject.lemb.spa.fl_str_mv Ciencias médicas - Innovaciones tecnológicas
Bioingeniería
description El glioblastoma multiforme (GBM) es uno de los tipos de cáncer con mayor letalidad. El promedio de vida para todos los pacientes es de 12-18 meses después del diagnóstico y tratamiento. Los principales tratamientos para combatir el glioblastoma son: la cirugía y radioterapia. La cirugía principalmente utilizada para eliminar la mayor parte de la masa tumoral, ha tenido limitaciones dado a la característica invasiva que precede del GBM. La radioterapia (RT) ha tomado un papel crucial en terapias de GBM, siendo su objetivo detener la proliferación celular ocasionando rupturas en la cadena del ADN de las células cancerosas. Sin embargo, las células de GBM tienen un carácter radioresistente, limitando la efectividad de esta terapia. Los puntos de carbono (PC) son nanoestructuras esféricas, de alta biocompatibilidad, propiedades ópticas y fisicoquímicas que los hacen interesantes para aplicaciones dirigidas al aumentar la efectividad de la radioterapia. En estas aplicaciones, uno de los parámetros más importantes a establecer en los PC es su nivel de citotoxicidad. Por esto, el objetivo de este trabajo fue identificar el efecto citotóxico que tienen los puntos de carbono a base de ácido cítrico frente a células cancerosas de glioblastoma (U87) y células cancerosas de mama (MCF-7) por medio de dos ensayos de viabilidad MTT y Azul Tripán. Como resultado se obtuvieron puntos de carbono por medio de reacción microondas (bottom up) a partir de acido cítrico, etanol y N,N-dimetilformamida, emitiendo una fluorescencia de color azul bajo irradiación con luz ultravioleta de 365 nm. Se vió una alta viabilidad de las células U87 y MCF-7 frente a los PC sintetizados. Lo que da a entender que su baja citotoxicidad evidenciada en este trabajo, su facilidad para modular propiedades superficiales y su biocompatibilidad hacen que los PC sean potencialmente investigados para trabajos futuros.
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-06-16T20:48:19Z
dc.date.available.none.fl_str_mv 2021-06-16T20:48:19Z
dc.date.created.none.fl_str_mv 2021-05-28
dc.type.eng.fl_str_mv bachelorThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.document.spa.fl_str_mv Trabajo de grado
dc.type.spa.spa.fl_str_mv Trabajo de grado
dc.identifier.doi.none.fl_str_mv https://doi.org/10.48713/10336_31625
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/31625
url https://doi.org/10.48713/10336_31625
https://repository.urosario.edu.co/handle/10336/31625
dc.language.iso.spa.fl_str_mv spa
language spa
dc.rights.*.fl_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
Abierto (Texto Completo)
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
http://purl.org/coar/access_right/c_abf2
dc.format.extent.spa.fl_str_mv 38 pp.
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.publisher.department.spa.fl_str_mv Escuela de Medicina y Ciencias de la Salud
dc.publisher.program.spa.fl_str_mv Ingeniería Biomédica
institution Universidad del Rosario
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spelling Rodríguez Burbano, Diana Consuelo52994699600Ondo Méndez, Alejandro Oyono79831981600Rodríguez Rojas, Yoly CarolinaIngeniero BiomédicoFull time4d6cdf11-c958-4fb2-bf27-ae3a7d52aecc6002021-06-16T20:48:19Z2021-06-16T20:48:19Z2021-05-28El glioblastoma multiforme (GBM) es uno de los tipos de cáncer con mayor letalidad. El promedio de vida para todos los pacientes es de 12-18 meses después del diagnóstico y tratamiento. Los principales tratamientos para combatir el glioblastoma son: la cirugía y radioterapia. La cirugía principalmente utilizada para eliminar la mayor parte de la masa tumoral, ha tenido limitaciones dado a la característica invasiva que precede del GBM. La radioterapia (RT) ha tomado un papel crucial en terapias de GBM, siendo su objetivo detener la proliferación celular ocasionando rupturas en la cadena del ADN de las células cancerosas. Sin embargo, las células de GBM tienen un carácter radioresistente, limitando la efectividad de esta terapia. Los puntos de carbono (PC) son nanoestructuras esféricas, de alta biocompatibilidad, propiedades ópticas y fisicoquímicas que los hacen interesantes para aplicaciones dirigidas al aumentar la efectividad de la radioterapia. En estas aplicaciones, uno de los parámetros más importantes a establecer en los PC es su nivel de citotoxicidad. Por esto, el objetivo de este trabajo fue identificar el efecto citotóxico que tienen los puntos de carbono a base de ácido cítrico frente a células cancerosas de glioblastoma (U87) y células cancerosas de mama (MCF-7) por medio de dos ensayos de viabilidad MTT y Azul Tripán. Como resultado se obtuvieron puntos de carbono por medio de reacción microondas (bottom up) a partir de acido cítrico, etanol y N,N-dimetilformamida, emitiendo una fluorescencia de color azul bajo irradiación con luz ultravioleta de 365 nm. Se vió una alta viabilidad de las células U87 y MCF-7 frente a los PC sintetizados. Lo que da a entender que su baja citotoxicidad evidenciada en este trabajo, su facilidad para modular propiedades superficiales y su biocompatibilidad hacen que los PC sean potencialmente investigados para trabajos futuros.Glioblastoma multiforme (GBM) is one of the most deadly cancers. The average life span for all patients is 12-18 months after diagnosis and treatment. The main treatments to combat glioblastoma are: surgery and radiation therapy. Surgery, mainly used to remove most of the tumor mass, has had limitations due to the invasive characteristic that precedes GBM. Radiotherapy (RT) has taken on a crucial role in GBM therapies, its objective being to stop cell proliferation by causing breaks in the DNA chain of cancer cells. However, GBM cells have a radioresistant character, limiting the effectiveness of this therapy. Carbon dots (PC) are spherical nanostructures, with high biocompatibility, optical and physicochemical properties that make them interesting for targeted applications by increasing the effectiveness of radiotherapy. In these applications, one of the most important parameters to establish in the PC is its level of cytotoxicity. Therefore, the objective of this work was to identify the cytotoxic effect that citric acid-based carbon spots have against glioblastoma cancer cells (U87) and breast cancer cells (MCF-7) by means of two viability assays. MTT and Azul Tripán. As a result, carbon points were obtained by means of microwave reaction (bottom up) from citric acid, ethanol and N, N-dimethylformamide, emitting a blue fluorescence under irradiation with 365 nm ultraviolet light. High viability of U87 and MCF-7 cells was seen against the synthesized PCs. This suggests that their low cytotoxicity evidenced in this work, their ease of modulating surface properties, and their biocompatibility make PCs potentially investigated for future work.38 pp.application/pdfhttps://doi.org/10.48713/10336_31625https://repository.urosario.edu.co/handle/10336/31625spaUniversidad del RosarioEscuela de Medicina y Ciencias de la SaludIngeniería BiomédicaAtribución-NoComercial-SinDerivadas 2.5 ColombiaAbierto (Texto Completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma.http://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2World Health Organization -cancer, (2018). https://www.who.int/healthtopics/cancer#tab=tab_1.G.P. Gupta, J. 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