Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2010
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/26336
- Acceso en línea:
- https://doi.org/10.1016/j.ibiod.2010.10.007
https://repository.urosario.edu.co/handle/10336/26336
- Palabra clave:
- Arsenate
arsC Gene
Indigenous microorganisms
Horizontal gene transfer (HGT)
- Rights
- License
- Restringido (Acceso a grupos específicos)
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oai:repository.urosario.edu.co:10336/26336 |
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EDOCUR2 |
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Repositorio EdocUR - U. Rosario |
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31c4c03a-b762-40c4-97df-9c9008c828dfec81d795-ff58-4860-941e-6e13f3e7faf079873757600dce58b5b-5d2b-4324-bf27-5b782fc79d1998eb8343-ccf8-4004-8c90-b0bb0218c7862020-08-06T16:21:21Z2020-08-06T16:21:21Z2010-11-18The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500 bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation–Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.application/pdfhttps://doi.org/10.1016/j.ibiod.2010.10.007ISSN: 0964-8305EISSN: 0265-3036https://repository.urosario.edu.co/handle/10336/26336engElsevier152No. 1147International Biodeterioration and BiodegradationVol. 65International Biodeterioration & Biodegradation, ISSN: 0964-8305;EISSN:0265-3036, Vol.65, No.1 (January 2011); pp.147-152https://www.sciencedirect.com/science/article/abs/pii/S0964830510001861?via%3DihubRestringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecInternational Biodeterioration and Biodegradationinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURArsenatearsC GeneIndigenous microorganismsHorizontal gene transfer (HGT)Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soilTransferencia horizontal de genes arsC entre microorganismos aislados del suelo contaminado con arsénicoarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Villegas-Torres, Maria F.Bedoya-Reina, Oscar C.Salazar, CamiloVives-Florez, Martha J.Dussan, Jenny10336/26336oai:repository.urosario.edu.co:10336/263362022-05-02 07:37:17.29675https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
dc.title.TranslatedTitle.spa.fl_str_mv |
Transferencia horizontal de genes arsC entre microorganismos aislados del suelo contaminado con arsénico |
title |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
spellingShingle |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil Arsenate arsC Gene Indigenous microorganisms Horizontal gene transfer (HGT) |
title_short |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
title_full |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
title_fullStr |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
title_full_unstemmed |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
title_sort |
Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil |
dc.subject.keyword.spa.fl_str_mv |
Arsenate arsC Gene Indigenous microorganisms Horizontal gene transfer (HGT) |
topic |
Arsenate arsC Gene Indigenous microorganisms Horizontal gene transfer (HGT) |
description |
The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500 bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation–Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time. |
publishDate |
2010 |
dc.date.created.spa.fl_str_mv |
2010-11-18 |
dc.date.accessioned.none.fl_str_mv |
2020-08-06T16:21:21Z |
dc.date.available.none.fl_str_mv |
2020-08-06T16:21:21Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.ibiod.2010.10.007 |
dc.identifier.issn.none.fl_str_mv |
ISSN: 0964-8305 EISSN: 0265-3036 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/26336 |
url |
https://doi.org/10.1016/j.ibiod.2010.10.007 https://repository.urosario.edu.co/handle/10336/26336 |
identifier_str_mv |
ISSN: 0964-8305 EISSN: 0265-3036 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
152 |
dc.relation.citationIssue.none.fl_str_mv |
No. 1 |
dc.relation.citationStartPage.none.fl_str_mv |
147 |
dc.relation.citationTitle.none.fl_str_mv |
International Biodeterioration and Biodegradation |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 65 |
dc.relation.ispartof.spa.fl_str_mv |
International Biodeterioration & Biodegradation, ISSN: 0964-8305;EISSN:0265-3036, Vol.65, No.1 (January 2011); pp.147-152 |
dc.relation.uri.spa.fl_str_mv |
https://www.sciencedirect.com/science/article/abs/pii/S0964830510001861?via%3Dihub |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_16ec |
dc.rights.acceso.spa.fl_str_mv |
Restringido (Acceso a grupos específicos) |
rights_invalid_str_mv |
Restringido (Acceso a grupos específicos) http://purl.org/coar/access_right/c_16ec |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Elsevier |
dc.source.spa.fl_str_mv |
International Biodeterioration and Biodegradation |
institution |
Universidad del Rosario |
dc.source.instname.none.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.none.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167664986161152 |