Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil

The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate...

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Autores:
Tipo de recurso:
Fecha de publicación:
2010
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26336
Acceso en línea:
https://doi.org/10.1016/j.ibiod.2010.10.007
https://repository.urosario.edu.co/handle/10336/26336
Palabra clave:
Arsenate
arsC Gene
Indigenous microorganisms
Horizontal gene transfer (HGT)
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Restringido (Acceso a grupos específicos)
id EDOCUR2_ba549668754d406bfca4b55267a2c9c6
oai_identifier_str oai:repository.urosario.edu.co:10336/26336
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 31c4c03a-b762-40c4-97df-9c9008c828dfec81d795-ff58-4860-941e-6e13f3e7faf079873757600dce58b5b-5d2b-4324-bf27-5b782fc79d1998eb8343-ccf8-4004-8c90-b0bb0218c7862020-08-06T16:21:21Z2020-08-06T16:21:21Z2010-11-18The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500 bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation–Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.application/pdfhttps://doi.org/10.1016/j.ibiod.2010.10.007ISSN: 0964-8305EISSN: 0265-3036https://repository.urosario.edu.co/handle/10336/26336engElsevier152No. 1147International Biodeterioration and BiodegradationVol. 65International Biodeterioration & Biodegradation, ISSN: 0964-8305;EISSN:0265-3036, Vol.65, No.1 (January 2011); pp.147-152https://www.sciencedirect.com/science/article/abs/pii/S0964830510001861?via%3DihubRestringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecInternational Biodeterioration and Biodegradationinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURArsenatearsC GeneIndigenous microorganismsHorizontal gene transfer (HGT)Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soilTransferencia horizontal de genes arsC entre microorganismos aislados del suelo contaminado con arsénicoarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Villegas-Torres, Maria F.Bedoya-Reina, Oscar C.Salazar, CamiloVives-Florez, Martha J.Dussan, Jenny10336/26336oai:repository.urosario.edu.co:10336/263362022-05-02 07:37:17.29675https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
dc.title.TranslatedTitle.spa.fl_str_mv Transferencia horizontal de genes arsC entre microorganismos aislados del suelo contaminado con arsénico
title Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
spellingShingle Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
Arsenate
arsC Gene
Indigenous microorganisms
Horizontal gene transfer (HGT)
title_short Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
title_full Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
title_fullStr Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
title_full_unstemmed Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
title_sort Horizontal arsC gene transfer among microorganisms isolated from arsenic polluted soil
dc.subject.keyword.spa.fl_str_mv Arsenate
arsC Gene
Indigenous microorganisms
Horizontal gene transfer (HGT)
topic Arsenate
arsC Gene
Indigenous microorganisms
Horizontal gene transfer (HGT)
description The study of recent evolution of the arsC genes amplified from microorganisms inhabiting a Colombian oil-polluted soil with high concentrations of arsenic was performed through the isolation of 26 bacterial morphotypes resistant to 10 mM of arsenate. A 353 bp fragment of the gene coding for arsenate-reductase enzyme (i.e. arsC), and a 500 bp 16S rDNA partial sequence were sequenced for 16 morphotypes of the 26 previously isolated. arsC sequences clustered on the same clade with previously reported arsC chromosomal genes of Escherichia coli and Shigella sp.; while 16S rDNA sequences grouped within the genus Pseudomonas and Bacillus. The GC content and the Codon Adaptation Index (CAI) were calculated and statistically compared, both supported the previous results. The Isolation–Migration model (IM model) was applied to calculate the genetic flux between each clade defined by the phylogenetic analysis. In general, the existence of recent horizontal gene transfer (HGT) events was confirmed, and the presence of the arsC gene in Bacillus sphaericus is reported for the first time.
publishDate 2010
dc.date.created.spa.fl_str_mv 2010-11-18
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:21:21Z
dc.date.available.none.fl_str_mv 2020-08-06T16:21:21Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.ibiod.2010.10.007
dc.identifier.issn.none.fl_str_mv ISSN: 0964-8305
EISSN: 0265-3036
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26336
url https://doi.org/10.1016/j.ibiod.2010.10.007
https://repository.urosario.edu.co/handle/10336/26336
identifier_str_mv ISSN: 0964-8305
EISSN: 0265-3036
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 152
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationStartPage.none.fl_str_mv 147
dc.relation.citationTitle.none.fl_str_mv International Biodeterioration and Biodegradation
dc.relation.citationVolume.none.fl_str_mv Vol. 65
dc.relation.ispartof.spa.fl_str_mv International Biodeterioration & Biodegradation, ISSN: 0964-8305;EISSN:0265-3036, Vol.65, No.1 (January 2011); pp.147-152
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/abs/pii/S0964830510001861?via%3Dihub
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Elsevier
dc.source.spa.fl_str_mv International Biodeterioration and Biodegradation
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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