A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease

Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic throughout Latin America and is spread by worldwide migration. Diagnosis is currently limited to serological and molecular techniques having variations regarding their sensitivity and specificity. This...

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Fecha de publicación:
2020
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23860
Acceso en línea:
https://doi.org/10.1155/2020/8074314
https://repository.urosario.edu.co/handle/10336/23860
Palabra clave:
Calcium binding protein
Ribosome rna
Satellite dna
Article
Chagas disease
Colorimetry
Controlled study
Cost effectiveness analysis
Cross reaction
Dna contamination
Dna template
Human
In vitro study
Limit of detection
Loop mediated isothermal amplification
Molecular diagnosis
Nonhuman
Nucleotide sequence
Point of care testing
Real time polymerase chain reaction
Trypanosoma cruzi
Rights
License
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spelling 2c84bb6d-b192-4c6a-b6c8-176cab915c41-1ec39620a-2cfa-42b2-b8b1-88a5fda77f85-1f07db193-acbe-40df-bb74-6161eba92c30-1b8ed28bd-76f4-4207-82c1-fd843ee895e0-1f27468c1-9def-45f3-bceb-097f59223890-18664af97-1c13-4eab-a68b-842a2d2aa5c2-15e6f806d-b29d-4ce2-a7b8-f8c430646216-17b6a9581-29ca-4406-9c84-84eb2eb4c7e8-123a60a9c-ca15-44ed-b129-758ff66f597c-15be4f0c6-d404-42a1-a0ab-b1779f6bbd3c-1796530656002020-05-26T00:06:08Z2020-05-26T00:06:08Z2020Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic throughout Latin America and is spread by worldwide migration. Diagnosis is currently limited to serological and molecular techniques having variations regarding their sensitivity and specificity. This work was aimed at developing a new sensitive, applicable, and cost-effective molecular diagnosis technique for loop-mediated isothermal amplification-based detection of T. cruzi (Tc-LAMP). The results led to determining a highly homologous satellite repeat region (231 bp) among parasite strains as a molecular marker for diagnosing the disease. Tc-LAMP was performed correctly for detecting parasite DNA (5 fg for the CL Brener strain and 50 fg for the DM28, TcVI, and TcI strains). Assay results proved negative for DNA from 16 helminth species and 7 protozoa, including Leishmania spp. Tc-LAMP based on the highly repeated T. cruzi satellite region is thus proposed as an important alternative for diagnosing T. cruzi infection, overcoming other methods' limitations such as their analytic capability, speed, and requiring specialized equipment or highly trained personnel. Tc-LAMP could be easily adapted for point-of-care testing in areas having limited resources. © 2020 Diego Ordóñez et al.application/pdfhttps://doi.org/10.1155/2020/80743142780240https://repository.urosario.edu.co/handle/10336/23860engHindawi LimitedDisease MarkersVol. 2020Disease Markers, ISSN:2780240, Vol.2020,(2020)https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081197087&doi=10.1155%2f2020%2f8074314&partnerID=40&md5=177cc2dfe20c680795dee37aeb04ee39Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURCalcium binding proteinRibosome rnaSatellite dnaArticleChagas diseaseColorimetryControlled studyCost effectiveness analysisCross reactionDna contaminationDna templateHumanIn vitro studyLimit of detectionLoop mediated isothermal amplificationMolecular diagnosisNonhumanNucleotide sequencePoint of care testingReal time polymerase chain reactionTrypanosoma cruziA Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' DiseasearticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Ordóñez, DiegoFernández-Soto, PedroFernández-Martín, Ana MCrego-Vicente, BeatrizFebrer-Sendra, BegoñaDiego, Juan García-BernaltVicente, BelénLópez-Abán, JulioBelhassen-García, MoncefMuro, AntonioPatarroyo, Manuel A.ORIGINAL8074314.pdfapplication/pdf964457https://repository.urosario.edu.co/bitstreams/ba880b18-b59a-43e6-8c4f-32189cd01bce/downloadb1cf3f9d1d3c3d7b8d432995782638e8MD51TEXT8074314.pdf.txt8074314.pdf.txtExtracted texttext/plain36284https://repository.urosario.edu.co/bitstreams/7014ddfa-a69a-4dbc-8850-260de6716296/download81de389fcb1d584f558d0e296f923744MD52THUMBNAIL8074314.pdf.jpg8074314.pdf.jpgGenerated Thumbnailimage/jpeg4450https://repository.urosario.edu.co/bitstreams/a092a22f-001a-4c9c-bcdb-38e055f3d924/download13d04a28e9b209644171a747712d9750MD5310336/23860oai:repository.urosario.edu.co:10336/238602022-05-02 07:37:21.24041https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
title A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
spellingShingle A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
Calcium binding protein
Ribosome rna
Satellite dna
Article
Chagas disease
Colorimetry
Controlled study
Cost effectiveness analysis
Cross reaction
Dna contamination
Dna template
Human
In vitro study
Limit of detection
Loop mediated isothermal amplification
Molecular diagnosis
Nonhuman
Nucleotide sequence
Point of care testing
Real time polymerase chain reaction
Trypanosoma cruzi
title_short A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
title_full A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
title_fullStr A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
title_full_unstemmed A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
title_sort A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas' Disease
dc.subject.keyword.spa.fl_str_mv Calcium binding protein
Ribosome rna
Satellite dna
Article
Chagas disease
Colorimetry
Controlled study
Cost effectiveness analysis
Cross reaction
Dna contamination
Dna template
Human
In vitro study
Limit of detection
Loop mediated isothermal amplification
Molecular diagnosis
Nonhuman
Nucleotide sequence
Point of care testing
Real time polymerase chain reaction
Trypanosoma cruzi
topic Calcium binding protein
Ribosome rna
Satellite dna
Article
Chagas disease
Colorimetry
Controlled study
Cost effectiveness analysis
Cross reaction
Dna contamination
Dna template
Human
In vitro study
Limit of detection
Loop mediated isothermal amplification
Molecular diagnosis
Nonhuman
Nucleotide sequence
Point of care testing
Real time polymerase chain reaction
Trypanosoma cruzi
description Chagas' disease is a neglected tropical disease caused by Trypanosoma cruzi which is endemic throughout Latin America and is spread by worldwide migration. Diagnosis is currently limited to serological and molecular techniques having variations regarding their sensitivity and specificity. This work was aimed at developing a new sensitive, applicable, and cost-effective molecular diagnosis technique for loop-mediated isothermal amplification-based detection of T. cruzi (Tc-LAMP). The results led to determining a highly homologous satellite repeat region (231 bp) among parasite strains as a molecular marker for diagnosing the disease. Tc-LAMP was performed correctly for detecting parasite DNA (5 fg for the CL Brener strain and 50 fg for the DM28, TcVI, and TcI strains). Assay results proved negative for DNA from 16 helminth species and 7 protozoa, including Leishmania spp. Tc-LAMP based on the highly repeated T. cruzi satellite region is thus proposed as an important alternative for diagnosing T. cruzi infection, overcoming other methods' limitations such as their analytic capability, speed, and requiring specialized equipment or highly trained personnel. Tc-LAMP could be easily adapted for point-of-care testing in areas having limited resources. © 2020 Diego Ordóñez et al.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:06:08Z
dc.date.available.none.fl_str_mv 2020-05-26T00:06:08Z
dc.date.created.spa.fl_str_mv 2020
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1155/2020/8074314
dc.identifier.issn.none.fl_str_mv 2780240
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23860
url https://doi.org/10.1155/2020/8074314
https://repository.urosario.edu.co/handle/10336/23860
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dc.language.iso.spa.fl_str_mv eng
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dc.relation.citationTitle.none.fl_str_mv Disease Markers
dc.relation.citationVolume.none.fl_str_mv Vol. 2020
dc.relation.ispartof.spa.fl_str_mv Disease Markers, ISSN:2780240, Vol.2020,(2020)
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rights_invalid_str_mv Abierto (Texto Completo)
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institution Universidad del Rosario
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