Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna

La identificación y caracterización de proteínas que utilizan los merozoitos de Plasmodium para invadir a su célula hospedera, representan una estrategia importante para desarrollar un método de control contra estos parásitos. A pesar de ello, la investigación básica en P. vivax está retrasada por s...

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Fecha de publicación:
2017
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Universidad del Rosario
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Repositorio EdocUR - U. Rosario
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spa
OAI Identifier:
oai:repository.urosario.edu.co:10336/19098
Acceso en línea:
https://doi.org/10.48713/10336_19098
http://repository.urosario.edu.co/handle/10336/19098
Palabra clave:
Plasmodium vivax
Invasión
Proteoma
Adhesión
Reticulocitos
Enfermedades
Plasmodium vivax
Invasion
Reticulocytes
Proteome
Characterization
Malaria
Plasmodium vivax
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License
Atribución-NoComercial-SinDerivadas 2.5 Colombia
id EDOCUR2_b23766045e700ee3bf99830f1146d23e
oai_identifier_str oai:repository.urosario.edu.co:10336/19098
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.spa.fl_str_mv Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
title Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
spellingShingle Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
Plasmodium vivax
Invasión
Proteoma
Adhesión
Reticulocitos
Enfermedades
Plasmodium vivax
Invasion
Reticulocytes
Proteome
Characterization
Malaria
Plasmodium vivax
title_short Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
title_full Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
title_fullStr Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
title_full_unstemmed Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
title_sort Determinación del proteoma de la cepa VCG-1 de Plasmodium Vivax y caracterización de moléculas candidatas para su inclusión en el desarrollo de una vacuna
dc.contributor.advisor.none.fl_str_mv Patarroyo, Manuel A.
Muro Álvarez, Antonio
dc.subject.spa.fl_str_mv Plasmodium vivax
Invasión
Proteoma
Adhesión
Reticulocitos
topic Plasmodium vivax
Invasión
Proteoma
Adhesión
Reticulocitos
Enfermedades
Plasmodium vivax
Invasion
Reticulocytes
Proteome
Characterization
Malaria
Plasmodium vivax
dc.subject.ddc.spa.fl_str_mv Enfermedades
dc.subject.keyword.spa.fl_str_mv Plasmodium vivax
Invasion
Reticulocytes
Proteome
Characterization
dc.subject.lemb.spa.fl_str_mv Malaria
Plasmodium vivax
description La identificación y caracterización de proteínas que utilizan los merozoitos de Plasmodium para invadir a su célula hospedera, representan una estrategia importante para desarrollar un método de control contra estos parásitos. A pesar de ello, la investigación básica en P. vivax está retrasada por su difícil propagación in vitro, debido a la preferencia que tiene el parásito por invadir reticulocitos, los cuales se encuentran en escaso porcentaje en sangre periférica de humanos adultos (1-2%) y son difíciles de obtener con alta pureza, en suficiente cantidad y totalmente viables. Como consecuencia de lo anterior, el conocimiento del número de moléculas que expresa P. vivax y cuáles de ellas son candidatas para componer una vacuna, es escaso. En este estudio, se evaluó el proteoma de una cepa de P. vivax adaptada a primates y se caracterizaron moléculas antigénicas y con capacidad de adhesión a reticulocitos humanos. En el análisis del proteoma de la cepa VCG-1 de P. vivax, se detectaron 734 proteínas, algunas esenciales en los pasos clave para establecer la invasión del merozoito a su célula diana. Además, se identificaron 811 componentes de eritrocitos (hospederos vitales de Plasmodium) del primate A. nancymaae, de los cuales 51 son proteínas integrales de membrana, 7 descritas como receptores de Plasmodium. Por otro lado, se identificó la presencia, transcripción y expresión de los genes codificantes de tres moléculas de P. vivax: PvARP, PvRBSA y PvGAMA, así como su antigenicidad. De particular interés, se encontró que PvRBSA y PvGAMA se unen en mayor proporción a reticulocitos que expresan el receptor CD71 de forma abundante (CD71hi), lo que sugiere que estas moléculas pueden estar participando en la selección preferencial que tienen los merozoitos de P. vivax por los reticulocitos humanos. Este es el primer estudio en Colombia donde se determina la composición proteica de una cepa de P. vivax adaptada a primates, así como la de eritrocitos de A. nancymaae. Como resultado más importante, se caracterizaron moléculas de P. vivax que son candidatos idóneos a ser evaluados como componentes de una vacuna contra la malaria causada por esta especie parasitaria.
publishDate 2017
dc.date.created.none.fl_str_mv 2017-09-07
dc.date.issued.none.fl_str_mv 2017
dc.date.accessioned.none.fl_str_mv 2019-02-18T21:46:17Z
dc.date.available.none.fl_str_mv 2019-02-18T21:46:17Z
dc.type.eng.fl_str_mv doctoralThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.document.spa.fl_str_mv Análisis de caso
dc.type.spa.spa.fl_str_mv Tesis de doctorado
dc.identifier.doi.none.fl_str_mv https://doi.org/10.48713/10336_19098
dc.identifier.uri.none.fl_str_mv http://repository.urosario.edu.co/handle/10336/19098
url https://doi.org/10.48713/10336_19098
http://repository.urosario.edu.co/handle/10336/19098
dc.language.iso.none.fl_str_mv spa
language spa
dc.rights.spa.fl_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
dc.rights.uri.none.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
rights_invalid_str_mv Atribución-NoComercial-SinDerivadas 2.5 Colombia
Abierto (Texto Completo)
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad del Rosario
dc.publisher.department.spa.fl_str_mv Facultad de Ciencias Naturales y Matemáticas
dc.publisher.program.spa.fl_str_mv Doctorado en Ciencias Biomédicas y Biológicas
institution Universidad del Rosario
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spelling Patarroyo, Manuel A.79653065600Muro Álvarez, Antonio8472e887-63aa-4a12-b4ec-e7ba5d5deac3600Moreno Pérez, Darwin AndrésDoctor en Ciencias Biomédicas y BiológicasFull timea933d343-0788-4635-9002-4943747140c36002019-02-18T21:46:17Z2019-02-18T21:46:17Z2017-09-072017La identificación y caracterización de proteínas que utilizan los merozoitos de Plasmodium para invadir a su célula hospedera, representan una estrategia importante para desarrollar un método de control contra estos parásitos. A pesar de ello, la investigación básica en P. vivax está retrasada por su difícil propagación in vitro, debido a la preferencia que tiene el parásito por invadir reticulocitos, los cuales se encuentran en escaso porcentaje en sangre periférica de humanos adultos (1-2%) y son difíciles de obtener con alta pureza, en suficiente cantidad y totalmente viables. Como consecuencia de lo anterior, el conocimiento del número de moléculas que expresa P. vivax y cuáles de ellas son candidatas para componer una vacuna, es escaso. En este estudio, se evaluó el proteoma de una cepa de P. vivax adaptada a primates y se caracterizaron moléculas antigénicas y con capacidad de adhesión a reticulocitos humanos. En el análisis del proteoma de la cepa VCG-1 de P. vivax, se detectaron 734 proteínas, algunas esenciales en los pasos clave para establecer la invasión del merozoito a su célula diana. Además, se identificaron 811 componentes de eritrocitos (hospederos vitales de Plasmodium) del primate A. nancymaae, de los cuales 51 son proteínas integrales de membrana, 7 descritas como receptores de Plasmodium. Por otro lado, se identificó la presencia, transcripción y expresión de los genes codificantes de tres moléculas de P. vivax: PvARP, PvRBSA y PvGAMA, así como su antigenicidad. De particular interés, se encontró que PvRBSA y PvGAMA se unen en mayor proporción a reticulocitos que expresan el receptor CD71 de forma abundante (CD71hi), lo que sugiere que estas moléculas pueden estar participando en la selección preferencial que tienen los merozoitos de P. vivax por los reticulocitos humanos. Este es el primer estudio en Colombia donde se determina la composición proteica de una cepa de P. vivax adaptada a primates, así como la de eritrocitos de A. nancymaae. Como resultado más importante, se caracterizaron moléculas de P. vivax que son candidatos idóneos a ser evaluados como componentes de una vacuna contra la malaria causada por esta especie parasitaria.Identifying and characterising proteins which use Plasmodium merozoites to invade host cells represents an important strategy for developing a method for controlling these parasites. However, basic P. vivax research has been delayed due to difficulties in propagating it in vitro as the parasite prefers to invade reticulocytes; there is a low percentage of these in adult human peripheral blood (1%-2%) and they are difficult to obtain with high purity, in a sufficient amount and totally viable. Consequently, knowledge is scarce regarding the amount of molecules being expressed by P. vivax and which of them represent good candidates for inclusion in an effective vaccine. This study has been aimed at evaluating the proteome of a primate-adapted P. vivax strain; antigenic molecules able to bind to human reticulocytes have been characterised. Analysing the P. vivax VCG-1 strain proteome led to detecting 734 proteins, some of them essential in key steps for establishing merozoite invasion of target cells. Furthermore, 811 A. nancymaae primate erythrocyte components (vital Plasmodium hosts) were identified; 51 of them were integral membrane proteins, 7 described as Plasmodium receptors. The presence, transcription, expression and antigenicity of genes encoding three P. vivax molecules (PvARP, PvRBSA and PvGAMA) were identified. Particularly interesting was the finding that a higher percentage of PvRBSA and PvGAMA bound to reticulocytes abundantly expressing the CD71 receptor (CD71hi), thereby suggesting that these molecules could be participating in P. vivax merozoite preferential selection for human reticulocytes. This the first study in Colombia which has determined the protein composition of a primate-adapted P. vivax strain as well as A. nancymaae erythrocytes. More importantly, P. vivax molecules were characterised which appear to be suitable candidates for being evaluated as components of a vaccine against malaria caused by the parasite species.Universidad del RosarioUniversidad de Salamancaapplication/pdfhttps://doi.org/10.48713/10336_19098 http://repository.urosario.edu.co/handle/10336/19098spaUniversidad del RosarioFacultad de Ciencias Naturales y MatemáticasDoctorado en Ciencias Biomédicas y BiológicasAtribución-NoComercial-SinDerivadas 2.5 ColombiaAbierto (Texto Completo)EL AUTOR, manifiesta que la obra objeto de la presente autorización es original y la realizó sin violar o usurpar derechos de autor de terceros, por lo tanto la obra es de exclusiva autoría y tiene la titularidad sobre la misma. PARGRAFO: En caso de presentarse cualquier reclamación o acción por parte de un tercero en cuanto a los derechos de autor sobre la obra en cuestión, EL AUTOR, asumirá toda la responsabilidad, y saldrá en defensa de los derechos aquí autorizados; para todos los efectos la universidad actúa como un tercero de buena fe. EL AUTOR, autoriza a LA UNIVERSIDAD DEL ROSARIO, para que en los términos establecidos en la Ley 23 de 1982, Ley 44 de 1993, Decisión andina 351 de 1993, Decreto 460 de 1995 y demás normas generales sobre la materia, utilice y use la obra objeto de la presente autorización. -------------------------------------- POLITICA DE TRATAMIENTO DE DATOS PERSONALES. Declaro que autorizo previa y de forma informada el tratamiento de mis datos personales por parte de LA UNIVERSIDAD DEL ROSARIO para fines académicos y en aplicación de convenios con terceros o servicios conexos con actividades propias de la academia, con estricto cumplimiento de los principios de ley. 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