Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was teste...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2008
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/23446
- Acceso en línea:
- https://doi.org/10.1016/j.vaccine.2008.05.092
https://repository.urosario.edu.co/handle/10336/23446
- Palabra clave:
- Membrane protein
Peptide vaccine
Amino acid sequence
Article
Bacterial gene
Cell line
Controlled study
Epithelium cell
Genetic transcription
Human
Human cell
Immunoelectron microscopy
Lung alveolus epithelium
Mycobacterium tuberculosis
Nonhuman
Peptide synthesis
Polymerase chain reaction
Priority journal
Protein binding
Protein localization
Rv1490 gene
Amino acid sequence
Animals
Bacterial adhesion
Bacterial proteins
Cell line
Epithelial cells
Membrane proteins
Molecular sequence data
Mycobacterium tuberculosis
Peptides
Protein binding
Rabbits
Rv1490
Tuberculosis
Vaccines
electron
transmission
Microscopy
- Rights
- License
- Abierto (Texto Completo)
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Repositorio EdocUR - U. Rosario |
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79653065-191225589-14f4f34c8-2d4c-491e-813c-5d61ee2d1048-16fc431a4-2889-4e78-ba01-10c2807c6557-180428f63-84aa-48a9-b8a4-c65b56ac3345-1f92a261c-8bfb-49fa-b4fc-3e5a502cd904-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-1518488266002020-05-26T00:02:04Z2020-05-26T00:02:04Z2008This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was tested, finding five high activity binding peptides (HABPs) for A549 and five for U937. Only two HABPs (11060 and 11073) were shared by both cell lines, both of which affected M. tuberculosis' invading ability to target cells, thus indicating an important role for these sequences in M. tuberculosis entry to A549 alveolar epithelial cells and supporting their inclusion in further studies on the development of a subunit-based multi-epitopic, chemically synthesised anti-tuberculosis vaccine. © 2008 Elsevier Ltd. All rights reserved.application/pdfhttps://doi.org/10.1016/j.vaccine.2008.05.0920264410X13588745https://repository.urosario.edu.co/handle/10336/23446eng4395No. 344387VaccineVol. 26Vaccine, ISSN:0264410X, 13588745, Vol.26, No.34 (2008); pp. 4387-4395https://www.scopus.com/inward/record.uri?eid=2-s2.0-47649132993&doi=10.1016%2fj.vaccine.2008.05.092&partnerID=40&md5=42f62e877e36c3c4e3846410ec1582e8Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMembrane proteinPeptide vaccineAmino acid sequenceArticleBacterial geneCell lineControlled studyEpithelium cellGenetic transcriptionHumanHuman cellImmunoelectron microscopyLung alveolus epitheliumMycobacterium tuberculosisNonhumanPeptide synthesisPolymerase chain reactionPriority journalProtein bindingProtein localizationRv1490 geneAmino acid sequenceAnimalsBacterial adhesionBacterial proteinsCell lineEpithelial cellsMembrane proteinsMolecular sequence dataMycobacterium tuberculosisPeptidesProtein bindingRabbitsRv1490TuberculosisVaccineselectrontransmissionMicroscopyPeptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Patarroyo, Manuel A.Curtidor, HernandoPlaza, David F.Reyes, ClaudiaSaboya, ObeimarBarrera, GloriaPatarroyo, Manuel E.Ocampo, Marisol10336/23446oai:repository.urosario.edu.co:10336/234462022-05-02 07:37:21.509644https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
title |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
spellingShingle |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? Membrane protein Peptide vaccine Amino acid sequence Article Bacterial gene Cell line Controlled study Epithelium cell Genetic transcription Human Human cell Immunoelectron microscopy Lung alveolus epithelium Mycobacterium tuberculosis Nonhuman Peptide synthesis Polymerase chain reaction Priority journal Protein binding Protein localization Rv1490 gene Amino acid sequence Animals Bacterial adhesion Bacterial proteins Cell line Epithelial cells Membrane proteins Molecular sequence data Mycobacterium tuberculosis Peptides Protein binding Rabbits Rv1490 Tuberculosis Vaccines electron transmission Microscopy |
title_short |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
title_full |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
title_fullStr |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
title_full_unstemmed |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
title_sort |
Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates? |
dc.subject.keyword.spa.fl_str_mv |
Membrane protein Peptide vaccine Amino acid sequence Article Bacterial gene Cell line Controlled study Epithelium cell Genetic transcription Human Human cell Immunoelectron microscopy Lung alveolus epithelium Mycobacterium tuberculosis Nonhuman Peptide synthesis Polymerase chain reaction Priority journal Protein binding Protein localization Rv1490 gene Amino acid sequence Animals Bacterial adhesion Bacterial proteins Cell line Epithelial cells Membrane proteins Molecular sequence data Mycobacterium tuberculosis Peptides Protein binding Rabbits Rv1490 Tuberculosis Vaccines |
topic |
Membrane protein Peptide vaccine Amino acid sequence Article Bacterial gene Cell line Controlled study Epithelium cell Genetic transcription Human Human cell Immunoelectron microscopy Lung alveolus epithelium Mycobacterium tuberculosis Nonhuman Peptide synthesis Polymerase chain reaction Priority journal Protein binding Protein localization Rv1490 gene Amino acid sequence Animals Bacterial adhesion Bacterial proteins Cell line Epithelial cells Membrane proteins Molecular sequence data Mycobacterium tuberculosis Peptides Protein binding Rabbits Rv1490 Tuberculosis Vaccines electron transmission Microscopy |
dc.subject.keyword.eng.fl_str_mv |
electron transmission Microscopy |
description |
This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was tested, finding five high activity binding peptides (HABPs) for A549 and five for U937. Only two HABPs (11060 and 11073) were shared by both cell lines, both of which affected M. tuberculosis' invading ability to target cells, thus indicating an important role for these sequences in M. tuberculosis entry to A549 alveolar epithelial cells and supporting their inclusion in further studies on the development of a subunit-based multi-epitopic, chemically synthesised anti-tuberculosis vaccine. © 2008 Elsevier Ltd. All rights reserved. |
publishDate |
2008 |
dc.date.created.spa.fl_str_mv |
2008 |
dc.date.accessioned.none.fl_str_mv |
2020-05-26T00:02:04Z |
dc.date.available.none.fl_str_mv |
2020-05-26T00:02:04Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.vaccine.2008.05.092 |
dc.identifier.issn.none.fl_str_mv |
0264410X 13588745 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/23446 |
url |
https://doi.org/10.1016/j.vaccine.2008.05.092 https://repository.urosario.edu.co/handle/10336/23446 |
identifier_str_mv |
0264410X 13588745 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
4395 |
dc.relation.citationIssue.none.fl_str_mv |
No. 34 |
dc.relation.citationStartPage.none.fl_str_mv |
4387 |
dc.relation.citationTitle.none.fl_str_mv |
Vaccine |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 26 |
dc.relation.ispartof.spa.fl_str_mv |
Vaccine, ISSN:0264410X, 13588745, Vol.26, No.34 (2008); pp. 4387-4395 |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-47649132993&doi=10.1016%2fj.vaccine.2008.05.092&partnerID=40&md5=42f62e877e36c3c4e3846410ec1582e8 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167680700121088 |