Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?

This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was teste...

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Autores:
Tipo de recurso:
Fecha de publicación:
2008
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23446
Acceso en línea:
https://doi.org/10.1016/j.vaccine.2008.05.092
https://repository.urosario.edu.co/handle/10336/23446
Palabra clave:
Membrane protein
Peptide vaccine
Amino acid sequence
Article
Bacterial gene
Cell line
Controlled study
Epithelium cell
Genetic transcription
Human
Human cell
Immunoelectron microscopy
Lung alveolus epithelium
Mycobacterium tuberculosis
Nonhuman
Peptide synthesis
Polymerase chain reaction
Priority journal
Protein binding
Protein localization
Rv1490 gene
Amino acid sequence
Animals
Bacterial adhesion
Bacterial proteins
Cell line
Epithelial cells
Membrane proteins
Molecular sequence data
Mycobacterium tuberculosis
Peptides
Protein binding
Rabbits
Rv1490
Tuberculosis
Vaccines
electron
transmission
Microscopy
Rights
License
Abierto (Texto Completo)
id EDOCUR2_b20c021cd9f9816cf13640353b5ea0bc
oai_identifier_str oai:repository.urosario.edu.co:10336/23446
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 79653065-191225589-14f4f34c8-2d4c-491e-813c-5d61ee2d1048-16fc431a4-2889-4e78-ba01-10c2807c6557-180428f63-84aa-48a9-b8a4-c65b56ac3345-1f92a261c-8bfb-49fa-b4fc-3e5a502cd904-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-1518488266002020-05-26T00:02:04Z2020-05-26T00:02:04Z2008This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was tested, finding five high activity binding peptides (HABPs) for A549 and five for U937. Only two HABPs (11060 and 11073) were shared by both cell lines, both of which affected M. tuberculosis' invading ability to target cells, thus indicating an important role for these sequences in M. tuberculosis entry to A549 alveolar epithelial cells and supporting their inclusion in further studies on the development of a subunit-based multi-epitopic, chemically synthesised anti-tuberculosis vaccine. © 2008 Elsevier Ltd. All rights reserved.application/pdfhttps://doi.org/10.1016/j.vaccine.2008.05.0920264410X13588745https://repository.urosario.edu.co/handle/10336/23446eng4395No. 344387VaccineVol. 26Vaccine, ISSN:0264410X, 13588745, Vol.26, No.34 (2008); pp. 4387-4395https://www.scopus.com/inward/record.uri?eid=2-s2.0-47649132993&doi=10.1016%2fj.vaccine.2008.05.092&partnerID=40&md5=42f62e877e36c3c4e3846410ec1582e8Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMembrane proteinPeptide vaccineAmino acid sequenceArticleBacterial geneCell lineControlled studyEpithelium cellGenetic transcriptionHumanHuman cellImmunoelectron microscopyLung alveolus epitheliumMycobacterium tuberculosisNonhumanPeptide synthesisPolymerase chain reactionPriority journalProtein bindingProtein localizationRv1490 geneAmino acid sequenceAnimalsBacterial adhesionBacterial proteinsCell lineEpithelial cellsMembrane proteinsMolecular sequence dataMycobacterium tuberculosisPeptidesProtein bindingRabbitsRv1490TuberculosisVaccineselectrontransmissionMicroscopyPeptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?articleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Patarroyo, Manuel A.Curtidor, HernandoPlaza, David F.Reyes, ClaudiaSaboya, ObeimarBarrera, GloriaPatarroyo, Manuel E.Ocampo, Marisol10336/23446oai:repository.urosario.edu.co:10336/234462022-05-02 07:37:21.509644https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
title Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
spellingShingle Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
Membrane protein
Peptide vaccine
Amino acid sequence
Article
Bacterial gene
Cell line
Controlled study
Epithelium cell
Genetic transcription
Human
Human cell
Immunoelectron microscopy
Lung alveolus epithelium
Mycobacterium tuberculosis
Nonhuman
Peptide synthesis
Polymerase chain reaction
Priority journal
Protein binding
Protein localization
Rv1490 gene
Amino acid sequence
Animals
Bacterial adhesion
Bacterial proteins
Cell line
Epithelial cells
Membrane proteins
Molecular sequence data
Mycobacterium tuberculosis
Peptides
Protein binding
Rabbits
Rv1490
Tuberculosis
Vaccines
electron
transmission
Microscopy
title_short Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
title_full Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
title_fullStr Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
title_full_unstemmed Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
title_sort Peptides derived from the Mycobacterium tuberculosis Rv1490 surface protein implicated in inhibition of epithelial cell entry: Potential vaccine candidates?
dc.subject.keyword.spa.fl_str_mv Membrane protein
Peptide vaccine
Amino acid sequence
Article
Bacterial gene
Cell line
Controlled study
Epithelium cell
Genetic transcription
Human
Human cell
Immunoelectron microscopy
Lung alveolus epithelium
Mycobacterium tuberculosis
Nonhuman
Peptide synthesis
Polymerase chain reaction
Priority journal
Protein binding
Protein localization
Rv1490 gene
Amino acid sequence
Animals
Bacterial adhesion
Bacterial proteins
Cell line
Epithelial cells
Membrane proteins
Molecular sequence data
Mycobacterium tuberculosis
Peptides
Protein binding
Rabbits
Rv1490
Tuberculosis
Vaccines
topic Membrane protein
Peptide vaccine
Amino acid sequence
Article
Bacterial gene
Cell line
Controlled study
Epithelium cell
Genetic transcription
Human
Human cell
Immunoelectron microscopy
Lung alveolus epithelium
Mycobacterium tuberculosis
Nonhuman
Peptide synthesis
Polymerase chain reaction
Priority journal
Protein binding
Protein localization
Rv1490 gene
Amino acid sequence
Animals
Bacterial adhesion
Bacterial proteins
Cell line
Epithelial cells
Membrane proteins
Molecular sequence data
Mycobacterium tuberculosis
Peptides
Protein binding
Rabbits
Rv1490
Tuberculosis
Vaccines
electron
transmission
Microscopy
dc.subject.keyword.eng.fl_str_mv electron
transmission
Microscopy
description This study reports the Rv1490 gene presence and transcription in members of the Mycobacterium tuberculosis complex, and characterises the encoded Rv1490 putative membrane protein in M. tuberculosis H37Rv. Rv1490 derived peptides were synthesised and their A549 and U937 cell binding ability was tested, finding five high activity binding peptides (HABPs) for A549 and five for U937. Only two HABPs (11060 and 11073) were shared by both cell lines, both of which affected M. tuberculosis' invading ability to target cells, thus indicating an important role for these sequences in M. tuberculosis entry to A549 alveolar epithelial cells and supporting their inclusion in further studies on the development of a subunit-based multi-epitopic, chemically synthesised anti-tuberculosis vaccine. © 2008 Elsevier Ltd. All rights reserved.
publishDate 2008
dc.date.created.spa.fl_str_mv 2008
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:02:04Z
dc.date.available.none.fl_str_mv 2020-05-26T00:02:04Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.vaccine.2008.05.092
dc.identifier.issn.none.fl_str_mv 0264410X
13588745
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23446
url https://doi.org/10.1016/j.vaccine.2008.05.092
https://repository.urosario.edu.co/handle/10336/23446
identifier_str_mv 0264410X
13588745
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 4395
dc.relation.citationIssue.none.fl_str_mv No. 34
dc.relation.citationStartPage.none.fl_str_mv 4387
dc.relation.citationTitle.none.fl_str_mv Vaccine
dc.relation.citationVolume.none.fl_str_mv Vol. 26
dc.relation.ispartof.spa.fl_str_mv Vaccine, ISSN:0264410X, 13588745, Vol.26, No.34 (2008); pp. 4387-4395
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-47649132993&doi=10.1016%2fj.vaccine.2008.05.092&partnerID=40&md5=42f62e877e36c3c4e3846410ec1582e8
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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