?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium
La actividad de la -galactosidasa refleja la tasa de envejecimiento celular in vitro. Mediante quimioluminiscencia se cuantificó dicha actividad a pH 6 en células epiteliales ováricas provenientes de 28 donantes sin antecedentes de cáncer. Las células fueron cultivadas en forma seriada hasta alcanza...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2010
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- spa
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/22187
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/22187
- Palabra clave:
- Beta galactosidase
Article
Cell culture
Enzyme activity
Epithelium cell
Female
Human
Human cell
In vitro study
Ovary cell
Senescence
Adolescent
Adult
Beta-galactosidase
Cell aging
Epithelial cells
Female
Humans
Middle aged
Ovary
Young adult
?-galactosidase
Biomarker
Ovarian epithelial cells
Replicative senescence
cultured
Cells
- Rights
- License
- Abierto (Texto Completo)
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2a23e975-7586-4a47-84da-b12513fc36efd8dfccad-b2c0-4aeb-ab60-570d3b5888a6ffa4f325-0ab6-46d9-82a7-17a5e2d1a4de79457397600518662516002020-05-25T23:55:43Z2020-05-25T23:55:43Z2010La actividad de la -galactosidasa refleja la tasa de envejecimiento celular in vitro. Mediante quimioluminiscencia se cuantificó dicha actividad a pH 6 en células epiteliales ováricas provenientes de 28 donantes sin antecedentes de cáncer. Las células fueron cultivadas en forma seriada hasta alcanzar el estado de detención permanente de crecimiento. Durante la fase de crecimiento exponencial, todos los cultivos mostraron un patrón semejante de crecimiento y una baja actividad -galactosidasa. Sin embargo, el inicio de la disminución de la capacidad replicativa que caracteriza el final de dicha fase, así como el inicio de la fase estacionaria o senescente presentaron un aumento significativo en la actividad enzimática. Nuestros resultados mostraron que la actividad -galactosidasa puede ser considerada como marcador de senescencia replicativa del epitelio superficial del ovario a pH 6.?-galactosidase activity reflects the rate of cellular aging in vitro. Such activity was quantified at pH 6 in ovarian epithelial cells from 28 donors without a history of cancer, by the chemoluminiscent method. The cells were serially cultured until they achieved the state of permanent growth arrest. During the exponential growth phase, all cultures showed a similar pattern of growth and low ?-galactosidase activity. However, both in the onset of decrease replicative activity, as well as in the onset of the stationary phase, there was a significant rise in the enzyme activity. Our results showed that ?-galactosidase activity can be considered as a replicative senescence marker of the ovarian surface epithelium at pH 6.application/pdf5355133https://repository.urosario.edu.co/handle/10336/22187spa367No. 3351Investigacion ClinicaVol. 51Investigacion Clinica, ISSN:5355133, Vol.51, No.3 (2010); pp. 351-367https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650437743&partnerID=40&md5=f83177e886dd4b79fb81810d8481812dAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURBeta galactosidaseArticleCell cultureEnzyme activityEpithelium cellFemaleHumanHuman cellIn vitro studyOvary cellSenescenceAdolescentAdultBeta-galactosidaseCell agingEpithelial cellsFemaleHumansMiddle agedOvaryYoung adult?-galactosidaseBiomarkerOvarian epithelial cellsReplicative senescenceculturedCells?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epitheliumActividad de la ?-galactosidasa como marcador de senescencia en cultivos primarios del epitelio superficial del ovarioarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Chuaire-Noack L.Rondón-Lagos S.Sánchez-Corredor M.Ibáñez Pinilla, MilcíadesRamírez Clavijo, Sandra RocíoORIGINAL28809-44188-1-PB.pdfapplication/pdf264650https://repository.urosario.edu.co/bitstreams/2e496048-9f97-43b5-9190-52b2862d9a20/download5749b751edd1203040b1e5757319afd5MD51TEXT28809-44188-1-PB.pdf.txt28809-44188-1-PB.pdf.txtExtracted texttext/plain53527https://repository.urosario.edu.co/bitstreams/69c0075f-7380-4be7-b80f-86ad54d9be9c/downloadea780c5442d18bc8bbc58be93d91174fMD52THUMBNAIL28809-44188-1-PB.pdf.jpg28809-44188-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg3910https://repository.urosario.edu.co/bitstreams/6e9b660c-cb61-49cb-9fe6-64ae0833bdfe/downloadbed81a9011560421b5526476424a717eMD5310336/22187oai:repository.urosario.edu.co:10336/221872022-05-02 07:37:16.619644https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
| dc.title.spa.fl_str_mv |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| dc.title.TranslatedTitle.spa.fl_str_mv |
Actividad de la ?-galactosidasa como marcador de senescencia en cultivos primarios del epitelio superficial del ovario |
| title |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| spellingShingle |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium Beta galactosidase Article Cell culture Enzyme activity Epithelium cell Female Human Human cell In vitro study Ovary cell Senescence Adolescent Adult Beta-galactosidase Cell aging Epithelial cells Female Humans Middle aged Ovary Young adult ?-galactosidase Biomarker Ovarian epithelial cells Replicative senescence cultured Cells |
| title_short |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| title_full |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| title_fullStr |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| title_full_unstemmed |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| title_sort |
?-galactosidase activity as a marker of senescence in primary cultures of the ovarian surface epithelium |
| dc.subject.keyword.spa.fl_str_mv |
Beta galactosidase Article Cell culture Enzyme activity Epithelium cell Female Human Human cell In vitro study Ovary cell Senescence Adolescent Adult Beta-galactosidase Cell aging Epithelial cells Female Humans Middle aged Ovary Young adult ?-galactosidase Biomarker Ovarian epithelial cells Replicative senescence |
| topic |
Beta galactosidase Article Cell culture Enzyme activity Epithelium cell Female Human Human cell In vitro study Ovary cell Senescence Adolescent Adult Beta-galactosidase Cell aging Epithelial cells Female Humans Middle aged Ovary Young adult ?-galactosidase Biomarker Ovarian epithelial cells Replicative senescence cultured Cells |
| dc.subject.keyword.eng.fl_str_mv |
cultured Cells |
| description |
La actividad de la -galactosidasa refleja la tasa de envejecimiento celular in vitro. Mediante quimioluminiscencia se cuantificó dicha actividad a pH 6 en células epiteliales ováricas provenientes de 28 donantes sin antecedentes de cáncer. Las células fueron cultivadas en forma seriada hasta alcanzar el estado de detención permanente de crecimiento. Durante la fase de crecimiento exponencial, todos los cultivos mostraron un patrón semejante de crecimiento y una baja actividad -galactosidasa. Sin embargo, el inicio de la disminución de la capacidad replicativa que caracteriza el final de dicha fase, así como el inicio de la fase estacionaria o senescente presentaron un aumento significativo en la actividad enzimática. Nuestros resultados mostraron que la actividad -galactosidasa puede ser considerada como marcador de senescencia replicativa del epitelio superficial del ovario a pH 6. |
| publishDate |
2010 |
| dc.date.created.spa.fl_str_mv |
2010 |
| dc.date.accessioned.none.fl_str_mv |
2020-05-25T23:55:43Z |
| dc.date.available.none.fl_str_mv |
2020-05-25T23:55:43Z |
| dc.type.eng.fl_str_mv |
article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_6501 |
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Artículo |
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5355133 |
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https://repository.urosario.edu.co/handle/10336/22187 |
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5355133 |
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https://repository.urosario.edu.co/handle/10336/22187 |
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spa |
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spa |
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367 |
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No. 3 |
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351 |
| dc.relation.citationTitle.none.fl_str_mv |
Investigacion Clinica |
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Vol. 51 |
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Investigacion Clinica, ISSN:5355133, Vol.51, No.3 (2010); pp. 351-367 |
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-78650437743&partnerID=40&md5=f83177e886dd4b79fb81810d8481812d |
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Abierto (Texto Completo) |
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Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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