Oxygen metabolism in human placenta mitochondria

Due to the high metabolic demands of the placental tissue during gestation, we decide to analyzed the mitochondrial bioenergetic functions in the human term placenta. Different mitochondrial morphological parameters, membrane potential and cardiolipin content were determined by flow cytometry. Oxyge...

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Tipo de recurso:
Fecha de publicación:
2014
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/25917
Acceso en línea:
https://doi.org/10.1007/s10863-014-9572-x
https://repository.urosario.edu.co/handle/10336/25917
Palabra clave:
Human placenta mitochondria
Free radicals
Hydrogen peroxide
Cardiolipin
Apoptosis
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Restringido (Acceso a grupos específicos)
id EDOCUR2_ae98d37faf508f614327efa7b29bf6cc
oai_identifier_str oai:repository.urosario.edu.co:10336/25917
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling bf28cd4a-5d01-493b-b1bc-677e1627a8a4-1dd720aeb-5f85-44cd-bc7d-58877ddc8b55-1aa714904-e4af-477a-9766-44ac2de01845-167becfe5-355d-48e1-afbd-1c3cebdb2daf-12ee800dc-1d4d-4e67-8bae-956b06ef4e4d-11770ebab-a7cd-4316-9c51-d6a5f12124ef-12020-08-06T16:20:12Z2020-08-06T16:20:12Z2014Due to the high metabolic demands of the placental tissue during gestation, we decide to analyzed the mitochondrial bioenergetic functions in the human term placenta. Different mitochondrial morphological parameters, membrane potential and cardiolipin content were determined by flow cytometry. Oxygen uptake, hydrogen peroxide production and cytochrome P450 content, were also measured. Some apoptotic mitochondrial proteins were also analyzed by western blot. Two isolated mitochondrial fractions were observed: large/heavy and small/light with different functional characteristics. Oxygen uptake showed a respiratory control (RC) of 3.4?±?0.3 for the heavy mitochondria, and 1.1?±?0.4 for light mitochondria, indicating a respiratory dysfunction in the light fraction. Good levels of polarization were detected in the heavy fraction, meanwhile the light population showed a collapsed ??m. Increased levels of cytochrome P450, higher levels of hydrogen peroxide, and low cardiolipin content were described for the light fraction. Three pro-apoptotic proteins p53, Bax, and cytochrome c were found increased in the heavy mitochondrial fraction; and deficient in the light fraction. The heavy mitochondrial fraction showed an improved respiratory function. This mitochondrial fraction, being probably from cytotrophoblast cells showed higher content of proteins able to induce apoptosis, indicating that these cells can effectively execute an apoptotic program in the presence of a death stimulus. Meanwhile the light and small organelles probably from syncytiotrophoblast, with a low oxygen metabolism, low level of ??m, and increased hydrogen peroxide production, may not actively perform an apoptotic process due to their deficient energetic level. This study contributes to the characterization of functional parameters of human placenta mitochondria in order to understand the oxygen metabolism during the physiological process of gestation.application/pdfhttps://doi.org/10.1007/s10863-014-9572-xISSN: 0145-479XEISSN: 1573-6881https://repository.urosario.edu.co/handle/10336/25917engSpringer Nature469No. 46459Journal of Bioenergetics and Biomembranes, Journal of BioenergeticsJournal of Bioenergetics and Biomembranes, Journal of Bioenergetics, ISSN:0145-479X;EISSN:1573-6881, No.46 (2014);pp.459-469https://link.springer.com/article/10.1007/s10863-014-9572-xRestringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecJournal of Bioenergetics and Biomembranes, Journal of Bioenergeticsinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURHuman placenta mitochondriaFree radicalsHydrogen peroxideCardiolipinApoptosisOxygen metabolism in human placenta mitochondriaMetabolismo del oxígeno en las mitocondrias de placenta humanaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Bustamante,JRamírez-Vélez,RCzerniczyniec,ACicerchia,DAguilar de Plata,A.CLores-Arnaiz,S10336/25917oai:repository.urosario.edu.co:10336/259172022-05-02 07:37:15.225428https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Oxygen metabolism in human placenta mitochondria
dc.title.TranslatedTitle.spa.fl_str_mv Metabolismo del oxígeno en las mitocondrias de placenta humana
title Oxygen metabolism in human placenta mitochondria
spellingShingle Oxygen metabolism in human placenta mitochondria
Human placenta mitochondria
Free radicals
Hydrogen peroxide
Cardiolipin
Apoptosis
title_short Oxygen metabolism in human placenta mitochondria
title_full Oxygen metabolism in human placenta mitochondria
title_fullStr Oxygen metabolism in human placenta mitochondria
title_full_unstemmed Oxygen metabolism in human placenta mitochondria
title_sort Oxygen metabolism in human placenta mitochondria
dc.subject.keyword.spa.fl_str_mv Human placenta mitochondria
Free radicals
Hydrogen peroxide
Cardiolipin
Apoptosis
topic Human placenta mitochondria
Free radicals
Hydrogen peroxide
Cardiolipin
Apoptosis
description Due to the high metabolic demands of the placental tissue during gestation, we decide to analyzed the mitochondrial bioenergetic functions in the human term placenta. Different mitochondrial morphological parameters, membrane potential and cardiolipin content were determined by flow cytometry. Oxygen uptake, hydrogen peroxide production and cytochrome P450 content, were also measured. Some apoptotic mitochondrial proteins were also analyzed by western blot. Two isolated mitochondrial fractions were observed: large/heavy and small/light with different functional characteristics. Oxygen uptake showed a respiratory control (RC) of 3.4?±?0.3 for the heavy mitochondria, and 1.1?±?0.4 for light mitochondria, indicating a respiratory dysfunction in the light fraction. Good levels of polarization were detected in the heavy fraction, meanwhile the light population showed a collapsed ??m. Increased levels of cytochrome P450, higher levels of hydrogen peroxide, and low cardiolipin content were described for the light fraction. Three pro-apoptotic proteins p53, Bax, and cytochrome c were found increased in the heavy mitochondrial fraction; and deficient in the light fraction. The heavy mitochondrial fraction showed an improved respiratory function. This mitochondrial fraction, being probably from cytotrophoblast cells showed higher content of proteins able to induce apoptosis, indicating that these cells can effectively execute an apoptotic program in the presence of a death stimulus. Meanwhile the light and small organelles probably from syncytiotrophoblast, with a low oxygen metabolism, low level of ??m, and increased hydrogen peroxide production, may not actively perform an apoptotic process due to their deficient energetic level. This study contributes to the characterization of functional parameters of human placenta mitochondria in order to understand the oxygen metabolism during the physiological process of gestation.
publishDate 2014
dc.date.created.spa.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2020-08-06T16:20:12Z
dc.date.available.none.fl_str_mv 2020-08-06T16:20:12Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1007/s10863-014-9572-x
dc.identifier.issn.none.fl_str_mv ISSN: 0145-479X
EISSN: 1573-6881
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/25917
url https://doi.org/10.1007/s10863-014-9572-x
https://repository.urosario.edu.co/handle/10336/25917
identifier_str_mv ISSN: 0145-479X
EISSN: 1573-6881
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 469
dc.relation.citationIssue.none.fl_str_mv No. 46
dc.relation.citationStartPage.none.fl_str_mv 459
dc.relation.citationTitle.none.fl_str_mv Journal of Bioenergetics and Biomembranes, Journal of Bioenergetics
dc.relation.ispartof.spa.fl_str_mv Journal of Bioenergetics and Biomembranes, Journal of Bioenergetics, ISSN:0145-479X;EISSN:1573-6881, No.46 (2014);pp.459-469
dc.relation.uri.spa.fl_str_mv https://link.springer.com/article/10.1007/s10863-014-9572-x
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer Nature
dc.source.spa.fl_str_mv Journal of Bioenergetics and Biomembranes, Journal of Bioenergetics
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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