A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development

The New World primate Aotus nancymaae is susceptible to infection by the human malaria parasite Plasmodium vivax and has therefore been recommended by the World Health Organization as a model for malaria vaccine candidate evaluation. We report the isolation, adaptation, titration and genetic charact...

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Autores:
Tipo de recurso:
Fecha de publicación:
2003
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27498
Acceso en línea:
https://doi.org/10.1016/S0264-410X(03)00278-0
https://repository.urosario.edu.co/handle/10336/27498
Palabra clave:
Aotus
Plasmodium vivax
Malaria
Rights
License
Restringido (Acceso a grupos específicos)
id EDOCUR2_ad9ef76be9a984b73e007dbe57aa0088
oai_identifier_str oai:repository.urosario.edu.co:10336/27498
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling da76e3f2-62a3-43a8-8ae0-2c7b70ab0847-13232d8d9-7ba7-4dfc-9c08-6a45ee7db484-1ecc2fd1e-5110-444c-a652-7006ce7b96c9-1a1e9724d-d464-4be5-b3bd-db3839982f82-1ee7d7bc3-af57-4745-9a8d-34409257e21a-1e0317cef-4a03-4663-9ff5-8ae683141140-1796530656002020-08-19T14:42:27Z2020-08-19T14:42:27Z2003-09-08The New World primate Aotus nancymaae is susceptible to infection by the human malaria parasite Plasmodium vivax and has therefore been recommended by the World Health Organization as a model for malaria vaccine candidate evaluation. We report the isolation, adaptation, titration and genetic characterization of a P. vivax wild strain in splenectomized A. nancymaae monkeys. Parasitemia remained high after 22 passages, reaching 7.88% by Giemsa and Acridine Orange staining and Real-Time PCR determination, making this P. vivax strain a highly infective and reliable asset to be used in P. vivax biological studies and vaccine development.application/pdfhttps://doi.org/10.1016/S0264-410X(03)00278-0ISSN: 0264-410XEISSN: 1873-2518https://repository.urosario.edu.co/handle/10336/27498engThe Japanese Society for VaccinologyElsevier3937No. 25-263930VaccineVol. 21Vaccine, ISSN: 0264-410X;EISSN: 1873-2518, Vol.21, No.25-26 (2003); pp. 3930-3937 https://www.sciencedirect.com/science/article/pii/S0264410X03002780?via%3DihubRestringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecVaccineinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURAotusPlasmodium vivaxMalariaA highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine developmentUna cepa de Plasmodium vivax altamente infecciosa adaptada a los monos Aotus: determinaciones hematológicas y moleculares cuantitativas útiles para el desarrollo de una vacuna contra el paludismo por P. vivaxarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Picode Coaña, YagoRodriguez, JosefaGuerrero, EduarBarrero, CarlosRodríguez, RaúlMendoza, MarcelaPatarroyo, Manuel A.10336/27498oai:repository.urosario.edu.co:10336/274982021-06-03 00:50:14.003https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
dc.title.TranslatedTitle.spa.fl_str_mv Una cepa de Plasmodium vivax altamente infecciosa adaptada a los monos Aotus: determinaciones hematológicas y moleculares cuantitativas útiles para el desarrollo de una vacuna contra el paludismo por P. vivax
title A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
spellingShingle A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
Aotus
Plasmodium vivax
Malaria
title_short A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
title_full A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
title_fullStr A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
title_full_unstemmed A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
title_sort A highly infective plasmodium vivax strain adapted to Aotus monkeys: quantitative haematological and molecular determinations useful for P. vivax malaria vaccine development
dc.subject.keyword.spa.fl_str_mv Aotus
Plasmodium vivax
Malaria
topic Aotus
Plasmodium vivax
Malaria
description The New World primate Aotus nancymaae is susceptible to infection by the human malaria parasite Plasmodium vivax and has therefore been recommended by the World Health Organization as a model for malaria vaccine candidate evaluation. We report the isolation, adaptation, titration and genetic characterization of a P. vivax wild strain in splenectomized A. nancymaae monkeys. Parasitemia remained high after 22 passages, reaching 7.88% by Giemsa and Acridine Orange staining and Real-Time PCR determination, making this P. vivax strain a highly infective and reliable asset to be used in P. vivax biological studies and vaccine development.
publishDate 2003
dc.date.created.spa.fl_str_mv 2003-09-08
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:42:27Z
dc.date.available.none.fl_str_mv 2020-08-19T14:42:27Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/S0264-410X(03)00278-0
dc.identifier.issn.none.fl_str_mv ISSN: 0264-410X
EISSN: 1873-2518
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27498
url https://doi.org/10.1016/S0264-410X(03)00278-0
https://repository.urosario.edu.co/handle/10336/27498
identifier_str_mv ISSN: 0264-410X
EISSN: 1873-2518
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 3937
dc.relation.citationIssue.none.fl_str_mv No. 25-26
dc.relation.citationStartPage.none.fl_str_mv 3930
dc.relation.citationTitle.none.fl_str_mv Vaccine
dc.relation.citationVolume.none.fl_str_mv Vol. 21
dc.relation.ispartof.spa.fl_str_mv Vaccine, ISSN: 0264-410X;EISSN: 1873-2518, Vol.21, No.25-26 (2003); pp. 3930-3937
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0264410X03002780?via%3Dihub
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv The Japanese Society for Vaccinology
Elsevier
dc.source.spa.fl_str_mv Vaccine
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167540956397568

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