Binding activity, structure, and immunogenicity of synthetic peptides derived from Plasmodium falciparum CelTOS and TRSP proteins

Several sporozoite proteins have been associated with Plasmodium falciparum cell traversal and hepatocyte invasion, including the cell-traversal protein for ookinetes and sporozoites (CelTOS), and thrombospondin-related sporozoite protein (TRSP). CelTOS and TRSP amino acid sequences have been finely mapped to identify regions specifically binding to HeLa and HepG2 cells, respectively. Three high-activity binding peptides (HABPs) were found in CelTOS and one HABP was found in TRSP, all of them having high ?-helical structure content. These HABPs' specific binding was sensitive to HeLa and HepG2 cells' pre-treatment with heparinase I and chondroitinase ABC. Despite their similarity at three-dimensional (3D) structural level, TRSP and TRAP HABPs located in the TSR domain did not compete for the same binding sites. CelTOS and TRSP HABPs were used as a template for designing modified sequences to then be assessed in the Aotus monkey experimental model. Antibodies directed against these modified HABPs were able to recognize both the native parasite protein by immunofluorescence assay and the recombinant protein (expressed in Escherichia coli) by Western blot and ELISA assays. The results suggested that these modified HABPs could be promising targets in designing a fully effective, antimalarial vaccine. © 2011 Springer-Verlag.

• Fecha de publicación: 2012
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/24263
• Acceso en línea: https://doi.org/10.1007/s00726-011-1087-8; https://repository.urosario.edu.co/handle/10336/24263
• Palabra clave: Cell traversal protein; Chondroitin abc lyase; Heparin lyase; High activity binding peptide; Malaria vaccine; Protozoal protein; Synthetic peptide; Thrombospondin related sporozoite protein; Unclassified drug; Alpha helix; Amino acid sequence; Animal experiment; Article; Binding site; Cell strain hepg2; Controlled study; Drug targeting; Enzyme linked immunosorbent assay; Haplorhini; Hela cell; Human; Human cell; Immunogenicity; Malaria falciparum; Nonhuman; Plasmodium falciparum; Priority journal; Protein binding; Protein domain; Protein structure; Western blotting; Amino acid sequence; Animals; Aotus trivirgatus; Binding sites; Chondroitin abc lyase; Hela cells; Hep g2 cells; Heparin lyase; Hepatocytes; Humans; Malaria vaccines; Peptides; Plasmodium falciparum; Protein binding; Protozoan proteins; Recombinant proteins; Sporozoites; Thrombospondins; Escherichia coli; Plasmodium falciparum; Celtos; Peptide; Plasmodium falciparum; Sporozoite; Trsp; Vaccine; secondary; tumor; Cell line; Protein structure
• License: Abierto (Texto Completo)