Transcriptomic analysis of FUCA1 knock-down in keratinocytes reveals new insights into the pathogenesis of fucosidosis skin lesions

Fucosidosis is a rare lysosomal storage disease which has been classified into two subtypes, depending on the severity of clinical signs and symptoms. Fucosidosis patients’ skin abnormalities include angiokeratoma corporis diffusum, widespread telangiectasia, thick skin, hyperhidrosis and hypohidrosis, acrocyanosis and distal transverse nail bands. It has been described that >50% of fucosidosis patients have angiokeratoma. At molecular level, fucosidosis is caused by lysosomal alpha-L-fucosidase (FUCA1) gene mutations. Obtaining samples for functional studies has been challenging due to the inherent difficulty in finding affected individuals. The effect of FUCA1 dysfunction on gene expression is unknown. The aim of this study was to analyse, in keratinocytes, the transcriptomic effect of FUCA1 knock-down for a better understanding of skin lesions’ pathogenesis affecting fucosidosis patients. FUCA1 knock-down (siRNA) was performed in human HaCaT immortalised keratinocytes. Affymetrix arrays and qPCR were used for analysing gene expression. Bioinformatics was used for functional clustering of modified genes. In total, 387 genes showed differential expression between FUCA1 silenced and non-silenced cells (222 up-regulated and 165 down-regulated). Up-regulated genes belonged to two major groups: keratinocyte differentiation/epidermal development (n = 17) and immune response (n = 61). Several transcription factors were up-regulated in FUCA1-siRNA transfected cells. This effect might partly have been produced by abnormal transcription factor expression, that is FOXN1. We thus propose that fucosidosis-related skin lesions (eg angiokeratoma) and those of other diseases (eg psoriasis) might be caused by dysfunctions in common aetiological overlapping molecular cascades. © 2018 John Wiley and Sons A/S. Published by John Wiley and Sons Ltd

• Fecha de publicación: 2018
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/24032
• Acceso en línea: https://doi.org/10.1111/exd.13532; https://repository.urosario.edu.co/handle/10336/24032
• Palabra clave: Alpha levo fucosidase; Rna; Small interfering rna; Transcription factor; Alpha levo fucosidase; Transcriptome; Article; Bioinformatics; Cell differentiation; Controlled study; Down regulation; Epidermis; Fuca1 gene; Fucosidosis; Gene cluster; Gene expression; Gene mutation; Gene silencing; Hacat cell line; Hemangiokeratoma; Histogenesis; Human; Human cell; Immune response; Keratinocyte; Molecular mechanics; Pathogenesis; Polymerase chain reaction; Protein expression; Quantitative analysis; Reverse transcription polymerase chain reaction; Rna extraction; Skin defect; Transcriptomics; Upregulation; Biology; Cell line; Complication; Dna microarray; Fucosidosis; Gene expression profiling; Gene knockdown; Genetics; Hemangiokeratoma; Immunology; Keratinocyte; Skin disease; Alpha-l-fucosidase; Angiokeratoma; Cell differentiation; Cell line; Computational biology; Down-regulation; Epidermis; Fucosidosis; Gene expression profiling; Gene knockdown techniques; Humans; Keratinocytes; Oligonucleotide array sequence analysis; Skin diseases; Transcriptome; Up-regulation; Angiokeratoma; Foxn1; Lysosomal alpha-l-fucosidase; Skin disease; Transcriptome; human; development and aging; Fuca1 protein; Growth
• License: Abierto (Texto Completo)