Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cell...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/24770
- Acceso en línea:
- https://repository.urosario.edu.co/handle/10336/24770
- Palabra clave:
- GRP78
Proteomics
Virus-cell interactions
Zika virus
chaperone
gallic acid
glucose
glucose regulated protein 78
honokiol
luciferase
messenger RNA
potassium channel
small interfering RNA
tolonium chloride
viral protein
virus nucleoprotein
antiviral activity
Article
cell viability
confocal microscopy
controlled study
gene expression
gene silencing
genetic transfection
human
human cell
immunoblotting
immunofluorescence test
immunoprecipitation
liquid chromatography-mass spectrometry
mass spectrometry
nonhuman
protein expression
protein homeostasis
protein protein interaction
protein synthesis
RNA synthesis
upregulation
virus detection
virus envelope
virus infectivity
virus load
virus release
virus replication
Western blotting
Zika fever
Zika virus
- Rights
- License
- Abierto (Texto Completo)
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oai:repository.urosario.edu.co:10336/24770 |
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8714dfcc-9e8f-4fc1-831e-2ec40dd75e94524835266007516e261-3134-46cd-a8df-1d983787bd2f358abec0-f9da-4cff-8426-29e91b2b9572ba8b09d7-06aa-4a90-b145-b6bc1787d44f194747786007b9431e8-1935-4e86-a838-902729c887c736a5559e-b096-4fbe-8206-7c67568742c04fa5d867-4566-4adf-849b-cad2902c83ad98d63c6c-f132-46f6-9b96-7ea5c3881bd92020-06-11T13:21:12Z2020-06-11T13:21:12Z2020Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cellular factors in the viral life cycle. Here, we investigated interactors of ZIKV envelope (E) protein by combining protein pull-down with mass spectrometry. We found that E interacts with the endoplasmic reticulum (ER) resident chaperone, glucose regulated protein 78 (GRP78). Although other flaviviruses are known to co-opt ER resident proteins, including GRP78, to enhance viral infectivity, the role ER proteins play during the ZIKV life cycle is yet to be elucidated. We showed that GRP78 levels increased during ZIKV infection and localised to sites coincident with ZIKV E staining. Depletion of GRP78 using specific siRNAs significantly reduced reporter-virus luciferase readings, viral protein synthesis, and viral titres. Additionally, GRP78 depletion reduced the ability of ZIKV to disrupt host cell translation and altered the localisation of viral replication factories, though there was no effect on viral RNA synthesis. In summary, we showed GRP78 is a vital host-factor during ZIKV infection, which may be involved in the coordination of viral replication factories. © 2020 by the authors.application/pdf19994915https://repository.urosario.edu.co/handle/10336/24770engMDPI AGNo. 5VirusesVol. 12Viruses, ISBN: 19994915, Vol.12, No.5 (2020); pp. -https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084627200&doi=10.3390%2fv12050524&partnerID=40&md5=930e36e2d92a6252452b9173fec17154Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURGRP78ProteomicsVirus-cell interactionsZika viruschaperonegallic acidglucoseglucose regulated protein 78honokiolluciferasemessenger RNApotassium channelsmall interfering RNAtolonium chlorideviral proteinvirus nucleoproteinantiviral activityArticlecell viabilityconfocal microscopycontrolled studygene expressiongene silencinggenetic transfectionhumanhuman cellimmunoblottingimmunofluorescence testimmunoprecipitationliquid chromatography-mass spectrometrymass spectrometrynonhumanprotein expressionprotein homeostasisprotein protein interactionprotein synthesisRNA synthesisupregulationvirus detectionvirus envelopevirus infectivityvirus loadvirus releasevirus replicationWestern blottingZika feverZika virusGlucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infectionarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Royle, J.Ramírez Santana, Heily CarolinaAkpunarlieva, S.Donald, C. L.Gestuveo, R. J.Anaya, Juan-ManuelMerits, A.Burchmore, R.Kohl, A.Varjak, M.10336/24770oai:repository.urosario.edu.co:10336/247702022-05-02 07:37:16.634689https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
title |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
spellingShingle |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection GRP78 Proteomics Virus-cell interactions Zika virus chaperone gallic acid glucose glucose regulated protein 78 honokiol luciferase messenger RNA potassium channel small interfering RNA tolonium chloride viral protein virus nucleoprotein antiviral activity Article cell viability confocal microscopy controlled study gene expression gene silencing genetic transfection human human cell immunoblotting immunofluorescence test immunoprecipitation liquid chromatography-mass spectrometry mass spectrometry nonhuman protein expression protein homeostasis protein protein interaction protein synthesis RNA synthesis upregulation virus detection virus envelope virus infectivity virus load virus release virus replication Western blotting Zika fever Zika virus |
title_short |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
title_full |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
title_fullStr |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
title_full_unstemmed |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
title_sort |
Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection |
dc.subject.keyword.spa.fl_str_mv |
GRP78 Proteomics Virus-cell interactions Zika virus chaperone gallic acid glucose glucose regulated protein 78 honokiol luciferase messenger RNA potassium channel small interfering RNA tolonium chloride viral protein virus nucleoprotein antiviral activity Article cell viability confocal microscopy controlled study gene expression gene silencing genetic transfection human human cell immunoblotting immunofluorescence test immunoprecipitation liquid chromatography-mass spectrometry mass spectrometry nonhuman protein expression protein homeostasis protein protein interaction protein synthesis RNA synthesis upregulation virus detection virus envelope virus infectivity virus load virus release virus replication Western blotting Zika fever Zika virus |
topic |
GRP78 Proteomics Virus-cell interactions Zika virus chaperone gallic acid glucose glucose regulated protein 78 honokiol luciferase messenger RNA potassium channel small interfering RNA tolonium chloride viral protein virus nucleoprotein antiviral activity Article cell viability confocal microscopy controlled study gene expression gene silencing genetic transfection human human cell immunoblotting immunofluorescence test immunoprecipitation liquid chromatography-mass spectrometry mass spectrometry nonhuman protein expression protein homeostasis protein protein interaction protein synthesis RNA synthesis upregulation virus detection virus envelope virus infectivity virus load virus release virus replication Western blotting Zika fever Zika virus |
description |
Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cellular factors in the viral life cycle. Here, we investigated interactors of ZIKV envelope (E) protein by combining protein pull-down with mass spectrometry. We found that E interacts with the endoplasmic reticulum (ER) resident chaperone, glucose regulated protein 78 (GRP78). Although other flaviviruses are known to co-opt ER resident proteins, including GRP78, to enhance viral infectivity, the role ER proteins play during the ZIKV life cycle is yet to be elucidated. We showed that GRP78 levels increased during ZIKV infection and localised to sites coincident with ZIKV E staining. Depletion of GRP78 using specific siRNAs significantly reduced reporter-virus luciferase readings, viral protein synthesis, and viral titres. Additionally, GRP78 depletion reduced the ability of ZIKV to disrupt host cell translation and altered the localisation of viral replication factories, though there was no effect on viral RNA synthesis. In summary, we showed GRP78 is a vital host-factor during ZIKV infection, which may be involved in the coordination of viral replication factories. © 2020 by the authors. |
publishDate |
2020 |
dc.date.accessioned.none.fl_str_mv |
2020-06-11T13:21:12Z |
dc.date.available.none.fl_str_mv |
2020-06-11T13:21:12Z |
dc.date.created.spa.fl_str_mv |
2020 |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.issn.none.fl_str_mv |
19994915 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/24770 |
identifier_str_mv |
19994915 |
url |
https://repository.urosario.edu.co/handle/10336/24770 |
dc.language.iso.none.fl_str_mv |
eng |
language |
eng |
dc.relation.citationIssue.none.fl_str_mv |
No. 5 |
dc.relation.citationTitle.none.fl_str_mv |
Viruses |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 12 |
dc.relation.ispartof.spa.fl_str_mv |
Viruses, ISBN: 19994915, Vol.12, No.5 (2020); pp. - |
dc.relation.uri.spa.fl_str_mv |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084627200&doi=10.3390%2fv12050524&partnerID=40&md5=930e36e2d92a6252452b9173fec17154 |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
dc.format.mimetype.none.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
MDPI AG |
institution |
Universidad del Rosario |
dc.source.instname.spa.fl_str_mv |
instname:Universidad del Rosario |
dc.source.reponame.spa.fl_str_mv |
reponame:Repositorio Institucional EdocUR |
repository.name.fl_str_mv |
Repositorio institucional EdocUR |
repository.mail.fl_str_mv |
edocur@urosario.edu.co |
_version_ |
1814167574982688768 |