Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection

Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cell...

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Autores:
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/24770
Acceso en línea:
https://repository.urosario.edu.co/handle/10336/24770
Palabra clave:
GRP78
Proteomics
Virus-cell interactions
Zika virus
chaperone
gallic acid
glucose
glucose regulated protein 78
honokiol
luciferase
messenger RNA
potassium channel
small interfering RNA
tolonium chloride
viral protein
virus nucleoprotein
antiviral activity
Article
cell viability
confocal microscopy
controlled study
gene expression
gene silencing
genetic transfection
human
human cell
immunoblotting
immunofluorescence test
immunoprecipitation
liquid chromatography-mass spectrometry
mass spectrometry
nonhuman
protein expression
protein homeostasis
protein protein interaction
protein synthesis
RNA synthesis
upregulation
virus detection
virus envelope
virus infectivity
virus load
virus release
virus replication
Western blotting
Zika fever
Zika virus
Rights
License
Abierto (Texto Completo)
id EDOCUR2_a70fdbb1c56aacdeaab4659a4374b0d1
oai_identifier_str oai:repository.urosario.edu.co:10336/24770
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 8714dfcc-9e8f-4fc1-831e-2ec40dd75e94524835266007516e261-3134-46cd-a8df-1d983787bd2f358abec0-f9da-4cff-8426-29e91b2b9572ba8b09d7-06aa-4a90-b145-b6bc1787d44f194747786007b9431e8-1935-4e86-a838-902729c887c736a5559e-b096-4fbe-8206-7c67568742c04fa5d867-4566-4adf-849b-cad2902c83ad98d63c6c-f132-46f6-9b96-7ea5c3881bd92020-06-11T13:21:12Z2020-06-11T13:21:12Z2020Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cellular factors in the viral life cycle. Here, we investigated interactors of ZIKV envelope (E) protein by combining protein pull-down with mass spectrometry. We found that E interacts with the endoplasmic reticulum (ER) resident chaperone, glucose regulated protein 78 (GRP78). Although other flaviviruses are known to co-opt ER resident proteins, including GRP78, to enhance viral infectivity, the role ER proteins play during the ZIKV life cycle is yet to be elucidated. We showed that GRP78 levels increased during ZIKV infection and localised to sites coincident with ZIKV E staining. Depletion of GRP78 using specific siRNAs significantly reduced reporter-virus luciferase readings, viral protein synthesis, and viral titres. Additionally, GRP78 depletion reduced the ability of ZIKV to disrupt host cell translation and altered the localisation of viral replication factories, though there was no effect on viral RNA synthesis. In summary, we showed GRP78 is a vital host-factor during ZIKV infection, which may be involved in the coordination of viral replication factories. © 2020 by the authors.application/pdf19994915https://repository.urosario.edu.co/handle/10336/24770engMDPI AGNo. 5VirusesVol. 12Viruses, ISBN: 19994915, Vol.12, No.5 (2020); pp. -https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084627200&doi=10.3390%2fv12050524&partnerID=40&md5=930e36e2d92a6252452b9173fec17154Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURGRP78ProteomicsVirus-cell interactionsZika viruschaperonegallic acidglucoseglucose regulated protein 78honokiolluciferasemessenger RNApotassium channelsmall interfering RNAtolonium chlorideviral proteinvirus nucleoproteinantiviral activityArticlecell viabilityconfocal microscopycontrolled studygene expressiongene silencinggenetic transfectionhumanhuman cellimmunoblottingimmunofluorescence testimmunoprecipitationliquid chromatography-mass spectrometrymass spectrometrynonhumanprotein expressionprotein homeostasisprotein protein interactionprotein synthesisRNA synthesisupregulationvirus detectionvirus envelopevirus infectivityvirus loadvirus releasevirus replicationWestern blottingZika feverZika virusGlucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infectionarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Royle, J.Ramírez Santana, Heily CarolinaAkpunarlieva, S.Donald, C. L.Gestuveo, R. J.Anaya, Juan-ManuelMerits, A.Burchmore, R.Kohl, A.Varjak, M.10336/24770oai:repository.urosario.edu.co:10336/247702022-05-02 07:37:16.634689https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
title Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
spellingShingle Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
GRP78
Proteomics
Virus-cell interactions
Zika virus
chaperone
gallic acid
glucose
glucose regulated protein 78
honokiol
luciferase
messenger RNA
potassium channel
small interfering RNA
tolonium chloride
viral protein
virus nucleoprotein
antiviral activity
Article
cell viability
confocal microscopy
controlled study
gene expression
gene silencing
genetic transfection
human
human cell
immunoblotting
immunofluorescence test
immunoprecipitation
liquid chromatography-mass spectrometry
mass spectrometry
nonhuman
protein expression
protein homeostasis
protein protein interaction
protein synthesis
RNA synthesis
upregulation
virus detection
virus envelope
virus infectivity
virus load
virus release
virus replication
Western blotting
Zika fever
Zika virus
title_short Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
title_full Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
title_fullStr Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
title_full_unstemmed Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
title_sort Glucose-regulated protein 78 interacts with zika virus envelope protein and contributes to a productive infection
dc.subject.keyword.spa.fl_str_mv GRP78
Proteomics
Virus-cell interactions
Zika virus
chaperone
gallic acid
glucose
glucose regulated protein 78
honokiol
luciferase
messenger RNA
potassium channel
small interfering RNA
tolonium chloride
viral protein
virus nucleoprotein
antiviral activity
Article
cell viability
confocal microscopy
controlled study
gene expression
gene silencing
genetic transfection
human
human cell
immunoblotting
immunofluorescence test
immunoprecipitation
liquid chromatography-mass spectrometry
mass spectrometry
nonhuman
protein expression
protein homeostasis
protein protein interaction
protein synthesis
RNA synthesis
upregulation
virus detection
virus envelope
virus infectivity
virus load
virus release
virus replication
Western blotting
Zika fever
Zika virus
topic GRP78
Proteomics
Virus-cell interactions
Zika virus
chaperone
gallic acid
glucose
glucose regulated protein 78
honokiol
luciferase
messenger RNA
potassium channel
small interfering RNA
tolonium chloride
viral protein
virus nucleoprotein
antiviral activity
Article
cell viability
confocal microscopy
controlled study
gene expression
gene silencing
genetic transfection
human
human cell
immunoblotting
immunofluorescence test
immunoprecipitation
liquid chromatography-mass spectrometry
mass spectrometry
nonhuman
protein expression
protein homeostasis
protein protein interaction
protein synthesis
RNA synthesis
upregulation
virus detection
virus envelope
virus infectivity
virus load
virus release
virus replication
Western blotting
Zika fever
Zika virus
description Zika virus (ZIKV; Flaviviridae) is a mosquito-borne flavivirus shown to cause fetal abnormalities collectively known as congenital Zika syndrome and Guillain-Barré syndrome in recent outbreaks. Currently, there is no specific treatment or vaccine available, and more effort is needed to identify cellular factors in the viral life cycle. Here, we investigated interactors of ZIKV envelope (E) protein by combining protein pull-down with mass spectrometry. We found that E interacts with the endoplasmic reticulum (ER) resident chaperone, glucose regulated protein 78 (GRP78). Although other flaviviruses are known to co-opt ER resident proteins, including GRP78, to enhance viral infectivity, the role ER proteins play during the ZIKV life cycle is yet to be elucidated. We showed that GRP78 levels increased during ZIKV infection and localised to sites coincident with ZIKV E staining. Depletion of GRP78 using specific siRNAs significantly reduced reporter-virus luciferase readings, viral protein synthesis, and viral titres. Additionally, GRP78 depletion reduced the ability of ZIKV to disrupt host cell translation and altered the localisation of viral replication factories, though there was no effect on viral RNA synthesis. In summary, we showed GRP78 is a vital host-factor during ZIKV infection, which may be involved in the coordination of viral replication factories. © 2020 by the authors.
publishDate 2020
dc.date.accessioned.none.fl_str_mv 2020-06-11T13:21:12Z
dc.date.available.none.fl_str_mv 2020-06-11T13:21:12Z
dc.date.created.spa.fl_str_mv 2020
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.issn.none.fl_str_mv 19994915
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/24770
identifier_str_mv 19994915
url https://repository.urosario.edu.co/handle/10336/24770
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 5
dc.relation.citationTitle.none.fl_str_mv Viruses
dc.relation.citationVolume.none.fl_str_mv Vol. 12
dc.relation.ispartof.spa.fl_str_mv Viruses, ISBN: 19994915, Vol.12, No.5 (2020); pp. -
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084627200&doi=10.3390%2fv12050524&partnerID=40&md5=930e36e2d92a6252452b9173fec17154
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv MDPI AG
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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