Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three...
- Autores:
- Tipo de recurso:
- Fecha de publicación:
- 2008
- Institución:
- Universidad del Rosario
- Repositorio:
- Repositorio EdocUR - U. Rosario
- Idioma:
- eng
- OAI Identifier:
- oai:repository.urosario.edu.co:10336/28156
- Acceso en línea:
- https://doi.org/10.1194/jlr.M800356-JLR200
https://repository.urosario.edu.co/handle/10336/28156
- Palabra clave:
- Gestation
Mucosal immunity
Conjugated linoleic acid
- Rights
- License
- Abierto (Texto Completo)
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b1a11603-1780-430d-b877-00ae151ab80152483526600a47dcb89-1f60-46db-bd4b-ad3e2eee0c78bfc414d3-1f02-43fb-b45b-180f4a714b8d27331a50-730b-4136-9048-9032a7d852ce766aab37-8e0a-4ef7-80a0-30e5a3b3aa1837126581-ba07-499a-b785-14b322dfae662020-08-19T14:46:09Z2020-08-19T14:46:09Z2008-09-29The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-day-old rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGF?, and PPAR? mRNA expression was measured in small intestine and colon by real time PCR, using Taqman® specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6- and 4-fold, respectively, and intestinal IgA protein by ?2-fold. TGF? gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPAR?, a possible mediator of CLA's effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats.application/pdfhttps://doi.org/10.1194/jlr.M800356-JLR200ISSN: 0022-2275EISSN: 1539-7262https://repository.urosario.edu.co/handle/10336/28156engAmerican Society for Biochemistry and Molecular Biology476No. 3467Journal of Lipid ResearchVol. 50Journal of Lipid Research, ISSN: 0022-2275;EISSN: 1539-7262, Vol.50, No.3 (March 2009); pp. 467-476https://www.jlr.org/content/50/3/467.full.pdf+htmlAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Journal of Lipid Researchinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURGestationMucosal immunityConjugated linoleic acidMucosal IgA increase in rats by continuous CLA feeding during suckling and early infancyAumento de IgA en la mucosa en ratas por alimentación continua con CLA durante la lactancia y la primera infanciaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Pérez-Cano, Francisco J.Ramírez Santana, Heily CarolinaMolero-Luís, MartaCastell, MargaridaRivero, MontserratCastellote, CristinaFranch, ÀngelsORIGINALJ-Lipid-Res-2009-Perez-Cano-467-76.pdfapplication/pdf1310029https://repository.urosario.edu.co/bitstreams/2eabeffd-36a8-42f7-a647-fd6665fc7a2f/download77d0cde2c81d235efa8fbffb8a941719MD51TEXTJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.txtJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.txtExtracted texttext/plain51486https://repository.urosario.edu.co/bitstreams/34f36fee-14af-461d-b5ff-cc90e190f3b8/download39d9db4bd5b9c3cd4808f5c3717b862dMD52THUMBNAILJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.jpgJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.jpgGenerated Thumbnailimage/jpeg4521https://repository.urosario.edu.co/bitstreams/7c57b747-7e3c-417b-add6-7de13a2d6267/downloade02b51960b99e782dadd3c54198fc00eMD5310336/28156oai:repository.urosario.edu.co:10336/281562021-10-06 23:18:21.681https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co |
dc.title.spa.fl_str_mv |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
dc.title.TranslatedTitle.spa.fl_str_mv |
Aumento de IgA en la mucosa en ratas por alimentación continua con CLA durante la lactancia y la primera infancia |
title |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
spellingShingle |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy Gestation Mucosal immunity Conjugated linoleic acid |
title_short |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
title_full |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
title_fullStr |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
title_full_unstemmed |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
title_sort |
Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy |
dc.subject.keyword.spa.fl_str_mv |
Gestation Mucosal immunity Conjugated linoleic acid |
topic |
Gestation Mucosal immunity Conjugated linoleic acid |
description |
The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-day-old rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGF?, and PPAR? mRNA expression was measured in small intestine and colon by real time PCR, using Taqman® specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6- and 4-fold, respectively, and intestinal IgA protein by ?2-fold. TGF? gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPAR?, a possible mediator of CLA's effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats. |
publishDate |
2008 |
dc.date.created.spa.fl_str_mv |
2008-09-29 |
dc.date.accessioned.none.fl_str_mv |
2020-08-19T14:46:09Z |
dc.date.available.none.fl_str_mv |
2020-08-19T14:46:09Z |
dc.type.eng.fl_str_mv |
article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.coar.fl_str_mv |
http://purl.org/coar/resource_type/c_6501 |
dc.type.spa.spa.fl_str_mv |
Artículo |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1194/jlr.M800356-JLR200 |
dc.identifier.issn.none.fl_str_mv |
ISSN: 0022-2275 EISSN: 1539-7262 |
dc.identifier.uri.none.fl_str_mv |
https://repository.urosario.edu.co/handle/10336/28156 |
url |
https://doi.org/10.1194/jlr.M800356-JLR200 https://repository.urosario.edu.co/handle/10336/28156 |
identifier_str_mv |
ISSN: 0022-2275 EISSN: 1539-7262 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.citationEndPage.none.fl_str_mv |
476 |
dc.relation.citationIssue.none.fl_str_mv |
No. 3 |
dc.relation.citationStartPage.none.fl_str_mv |
467 |
dc.relation.citationTitle.none.fl_str_mv |
Journal of Lipid Research |
dc.relation.citationVolume.none.fl_str_mv |
Vol. 50 |
dc.relation.ispartof.spa.fl_str_mv |
Journal of Lipid Research, ISSN: 0022-2275;EISSN: 1539-7262, Vol.50, No.3 (March 2009); pp. 467-476 |
dc.relation.uri.spa.fl_str_mv |
https://www.jlr.org/content/50/3/467.full.pdf+html |
dc.rights.coar.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.acceso.spa.fl_str_mv |
Abierto (Texto Completo) |
rights_invalid_str_mv |
Abierto (Texto Completo) http://purl.org/coar/access_right/c_abf2 |
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application/pdf |
dc.publisher.spa.fl_str_mv |
American Society for Biochemistry and Molecular Biology |
dc.source.spa.fl_str_mv |
Journal of Lipid Research |
institution |
Universidad del Rosario |
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reponame:Repositorio Institucional EdocUR |
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