Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy

The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three...

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Tipo de recurso:
Fecha de publicación:
2008
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/28156
Acceso en línea:
https://doi.org/10.1194/jlr.M800356-JLR200
https://repository.urosario.edu.co/handle/10336/28156
Palabra clave:
Gestation
Mucosal immunity
Conjugated linoleic acid
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spelling b1a11603-1780-430d-b877-00ae151ab80152483526600a47dcb89-1f60-46db-bd4b-ad3e2eee0c78bfc414d3-1f02-43fb-b45b-180f4a714b8d27331a50-730b-4136-9048-9032a7d852ce766aab37-8e0a-4ef7-80a0-30e5a3b3aa1837126581-ba07-499a-b785-14b322dfae662020-08-19T14:46:09Z2020-08-19T14:46:09Z2008-09-29The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-day-old rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGF?, and PPAR? mRNA expression was measured in small intestine and colon by real time PCR, using Taqman® specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6- and 4-fold, respectively, and intestinal IgA protein by ?2-fold. TGF? gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPAR?, a possible mediator of CLA's effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats.application/pdfhttps://doi.org/10.1194/jlr.M800356-JLR200ISSN: 0022-2275EISSN: 1539-7262https://repository.urosario.edu.co/handle/10336/28156engAmerican Society for Biochemistry and Molecular Biology476No. 3467Journal of Lipid ResearchVol. 50Journal of Lipid Research, ISSN: 0022-2275;EISSN: 1539-7262, Vol.50, No.3 (March 2009); pp. 467-476https://www.jlr.org/content/50/3/467.full.pdf+htmlAbierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Journal of Lipid Researchinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURGestationMucosal immunityConjugated linoleic acidMucosal IgA increase in rats by continuous CLA feeding during suckling and early infancyAumento de IgA en la mucosa en ratas por alimentación continua con CLA durante la lactancia y la primera infanciaarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Pérez-Cano, Francisco J.Ramírez Santana, Heily CarolinaMolero-Luís, MartaCastell, MargaridaRivero, MontserratCastellote, CristinaFranch, ÀngelsORIGINALJ-Lipid-Res-2009-Perez-Cano-467-76.pdfapplication/pdf1310029https://repository.urosario.edu.co/bitstreams/2eabeffd-36a8-42f7-a647-fd6665fc7a2f/download77d0cde2c81d235efa8fbffb8a941719MD51TEXTJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.txtJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.txtExtracted texttext/plain51486https://repository.urosario.edu.co/bitstreams/34f36fee-14af-461d-b5ff-cc90e190f3b8/download39d9db4bd5b9c3cd4808f5c3717b862dMD52THUMBNAILJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.jpgJ-Lipid-Res-2009-Perez-Cano-467-76.pdf.jpgGenerated Thumbnailimage/jpeg4521https://repository.urosario.edu.co/bitstreams/7c57b747-7e3c-417b-add6-7de13a2d6267/downloade02b51960b99e782dadd3c54198fc00eMD5310336/28156oai:repository.urosario.edu.co:10336/281562021-10-06 23:18:21.681https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
dc.title.TranslatedTitle.spa.fl_str_mv Aumento de IgA en la mucosa en ratas por alimentación continua con CLA durante la lactancia y la primera infancia
title Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
spellingShingle Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
Gestation
Mucosal immunity
Conjugated linoleic acid
title_short Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
title_full Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
title_fullStr Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
title_full_unstemmed Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
title_sort Mucosal IgA increase in rats by continuous CLA feeding during suckling and early infancy
dc.subject.keyword.spa.fl_str_mv Gestation
Mucosal immunity
Conjugated linoleic acid
topic Gestation
Mucosal immunity
Conjugated linoleic acid
description The aim of this work was to establish the effect of the cis9,trans11 conjugated linoleic acid (CLA) isomer on mucosal immunity during early life in rats, a period when mucosal immunoglobulin production is poorly developed, as is also the case in humans. CLA supplementation was performed during three life periods: gestation, suckling, and early infancy. The immune status of supplemented animals was evaluated at two time points: at the end of the suckling period (21-day-old rats) and 1 week after weaning (28-day-old rats). Secretory IgA was quantified in intestinal washes from 28-day-old rats by ELISA technique. IgA, TGF?, and PPAR? mRNA expression was measured in small intestine and colon by real time PCR, using Taqman® specific probes and primers. IgA mucosal production was enhanced in animals supplemented with CLA during suckling and early infancy: in 28-day-old rats, IgA mRNA expression was increased in small intestine and colon by approximately 6- and 4-fold, respectively, and intestinal IgA protein by ?2-fold. TGF? gene expression was independent of age and type of tissue considered, and was not modified by dietary CLA. Gene expression of PPAR?, a possible mediator of CLA's effects was also upregulated in animals receiving CLA during early life. In conclusion, dietary supplementation with CLA during suckling and extended to early infancy enhances development of the intestinal immune response in rats.
publishDate 2008
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dc.date.accessioned.none.fl_str_mv 2020-08-19T14:46:09Z
dc.date.available.none.fl_str_mv 2020-08-19T14:46:09Z
dc.type.eng.fl_str_mv article
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dc.type.spa.spa.fl_str_mv Artículo
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dc.identifier.issn.none.fl_str_mv ISSN: 0022-2275
EISSN: 1539-7262
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url https://doi.org/10.1194/jlr.M800356-JLR200
https://repository.urosario.edu.co/handle/10336/28156
identifier_str_mv ISSN: 0022-2275
EISSN: 1539-7262
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 476
dc.relation.citationIssue.none.fl_str_mv No. 3
dc.relation.citationStartPage.none.fl_str_mv 467
dc.relation.citationTitle.none.fl_str_mv Journal of Lipid Research
dc.relation.citationVolume.none.fl_str_mv Vol. 50
dc.relation.ispartof.spa.fl_str_mv Journal of Lipid Research, ISSN: 0022-2275;EISSN: 1539-7262, Vol.50, No.3 (March 2009); pp. 467-476
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dc.publisher.spa.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.spa.fl_str_mv Journal of Lipid Research
institution Universidad del Rosario
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