Structural and immunological principles leading to chemically synthesized, multiantigenic, multistage, minimal subunit-based vaccine development

Identifying the principles and rules for developing a logical, rational vaccine methodology against various diseases, is discussed. SPf66, the first multiantigenic, multistage, minimal subunit-based, chemically synthesized anti-P. falciparum malaria vaccine. A robust, sensitive, and specific methodology is developed for defining the intimate molecular interactions mediating merozoite invasion of RBC by synthesizing short merozoite-derived protein peptides binding specifically and with high affinity (HABP) to RBCs. Conserved HABP-mediated sporozoite binding to hepatic cells is identified in EBA-175 for developing a multiantigenic, multistage, fully protective antimalarial vaccine. for developing a logical and rational vaccine methodology at the molecular level, monkeys' immune system molecules is analyzed by cloning and sequencing the Aotus genes encoding immunoglobulins, cytokines, and Class I and II molecules.

• Fecha de publicación: 2011
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23654
• Acceso en línea: https://doi.org/10.1021/cr100223m; https://repository.urosario.edu.co/handle/10336/23654
• Palabra clave: Class I; Cloning and sequencing; Cytokines; Falciparum malaria; Genes encoding; Hepatic cells; High affinity; Immune systems; Molecular levels; Vaccine development; Biosynthesis; Cloning; Genes; Vaccines; Subunit vaccine; Vaccine; Chemical structure; Human; Immunization; Immunology; Review; Humans; Immunization; Vaccines; Subunit; Molecular; Models; Vaccines
• License: Abierto (Texto Completo)