Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines

The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT?PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic...

Full description

Autores:
Tipo de recurso:
Fecha de publicación:
2005
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/26623
Acceso en línea:
https://doi.org/10.1110/ps.051526305
https://repository.urosario.edu.co/handle/10336/26623
Palabra clave:
Mycobacterium tuberculosis
High activity binding peptides
Receptor–ligand interaction
Mycobacterium tuberculosis complex
HABP
high activity binding peptide
BS3
bis?sulfosuccinimidyl suberate
HBS
hepes buffer saline
SEC size exclusion chromatography
Rights
License
Abierto (Texto Completo)
id EDOCUR2_a0c3fd146f19a8d49dcb60cc5707b4f5
oai_identifier_str oai:repository.urosario.edu.co:10336/26623
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling a4e7ff19-544a-44fe-ad9c-61d4aff3b8baeba5265c-2b9f-49a4-8c8c-4e3459a41e199122558934b82e95-53e0-4ba5-a5c5-d4191164afa1cb4fb759-401e-4b06-8a94-5315ca1cd402600518488266002020-08-19T14:39:54Z2020-08-19T14:39:54Z2005-09The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT?PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25?kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20?amino?acid?long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross?linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.application/pdfhttps://doi.org/10.1110/ps.051526305ISSN: 0961-8368EISSN: 1469-896Xhttps://repository.urosario.edu.co/handle/10336/26623engThe Protein SocietyJohn Wiley & Son2245No. 92236Protein ScienceVol. 14Protein Science, ISSN: 0961-8368;EISSN: 1469-896X, Vol.14, No.9 (September 2005); pp. 2236-2245https://onlinelibrary.wiley.com/doi/epdf/10.1110/ps.051526305Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2Protein Scienceinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMycobacterium tuberculosisHigh activity binding peptidesReceptor–ligand interactionMycobacterium tuberculosis complexHABPhigh activity binding peptideBS3bis?sulfosuccinimidyl suberateHBShepes buffer salineSEC size exclusion chromatographyMycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell linesProteína de Mycobacterium tuberculosis Rv2536 implicada en la unión específica a líneas celulares humanasarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501García, JavierPuentes, AlvaroCurtidor, HernandoVera, RicardoRodríguez, LuisOcampo, Marisol10336/26623oai:repository.urosario.edu.co:10336/266232022-05-02 07:37:17.52774https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
dc.title.TranslatedTitle.spa.fl_str_mv Proteína de Mycobacterium tuberculosis Rv2536 implicada en la unión específica a líneas celulares humanas
title Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
spellingShingle Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
Mycobacterium tuberculosis
High activity binding peptides
Receptor–ligand interaction
Mycobacterium tuberculosis complex
HABP
high activity binding peptide
BS3
bis?sulfosuccinimidyl suberate
HBS
hepes buffer saline
SEC size exclusion chromatography
title_short Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
title_full Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
title_fullStr Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
title_full_unstemmed Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
title_sort Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
dc.subject.keyword.spa.fl_str_mv Mycobacterium tuberculosis
High activity binding peptides
Receptor–ligand interaction
Mycobacterium tuberculosis complex
HABP
high activity binding peptide
BS3
bis?sulfosuccinimidyl suberate
HBS
hepes buffer saline
SEC size exclusion chromatography
topic Mycobacterium tuberculosis
High activity binding peptides
Receptor–ligand interaction
Mycobacterium tuberculosis complex
HABP
high activity binding peptide
BS3
bis?sulfosuccinimidyl suberate
HBS
hepes buffer saline
SEC size exclusion chromatography
description The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT?PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25?kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20?amino?acid?long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross?linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.
publishDate 2005
dc.date.created.spa.fl_str_mv 2005-09
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:39:54Z
dc.date.available.none.fl_str_mv 2020-08-19T14:39:54Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1110/ps.051526305
dc.identifier.issn.none.fl_str_mv ISSN: 0961-8368
EISSN: 1469-896X
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/26623
url https://doi.org/10.1110/ps.051526305
https://repository.urosario.edu.co/handle/10336/26623
identifier_str_mv ISSN: 0961-8368
EISSN: 1469-896X
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 2245
dc.relation.citationIssue.none.fl_str_mv No. 9
dc.relation.citationStartPage.none.fl_str_mv 2236
dc.relation.citationTitle.none.fl_str_mv Protein Science
dc.relation.citationVolume.none.fl_str_mv Vol. 14
dc.relation.ispartof.spa.fl_str_mv Protein Science, ISSN: 0961-8368;EISSN: 1469-896X, Vol.14, No.9 (September 2005); pp. 2236-2245
dc.relation.uri.spa.fl_str_mv https://onlinelibrary.wiley.com/doi/epdf/10.1110/ps.051526305
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv The Protein Society
John Wiley & Son
dc.source.spa.fl_str_mv Protein Science
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167738673790976

Publicaciones similares