Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases

Fe de erratas. The fifth author's name is spelled incorrectly. The correct name is: Carlos A. Valencia-Hernandez. The correct citation is: Hernández C, Cucunubá Z, Flórez C, Olivera M, Valencia-Hernandez C.A., Zambrano P, et al. (2016) Molecular Diagnosis of Chagas Disease in Colombia: Parasiti...

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Fecha de publicación:: 2016

Institución:: Universidad del Rosario

Repositorio:: Repositorio EdocUR - U. Rosario

Idioma:: eng

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dc.title.spa.fl_str_mv	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
title	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
spellingShingle	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases Molecular Marker Protozoal Dna Spacer Dna Area Under The Curve Blood Culture Blood Smear Chagas Disease Colombia Diagnostic Accuracy Diagnostic Test Accuracy Study Discrete Typing Unit Disease Course Enzyme Linked Immunosorbent Assay Genetic Variability Genotype Hemagglutination Inhibition Test Human Immunofluorescence Test Male Microorganism Detection Molecular Diagnosis Nonhuman Parasite Load Parasitemia Predictive Value Real Time Polymerase Chain Reaction Receiver Operating Characteristic Sensitivity And Specificity Trypanosoma Cruzi Acute Disease Chagas Disease Chronic Disease Comparative Study Exon Genetic Variation Isolation And Purification Molecular Typing Parasite Load Young Adult Acute Disease Chagas Disease Chronic Disease Colombia Dna, Protozoan Exons Genetic Variation Genotype Male Molecular Typing Parasite Load Real-Time Polymerase Chain Reaction Sensitivity And Specificity Trypanosoma Cruzi Young Adult Enfermedades Adult Article Female Middle Aged Adult Female Humans Middle Aged Enfermedad de chagas Parasitemia Trypanosoma cruzi
title_short	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
title_full	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
title_fullStr	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
title_full_unstemmed	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
title_sort	Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases
dc.subject.spa.fl_str_mv	Molecular Marker Protozoal Dna Spacer Dna Area Under The Curve Blood Culture Blood Smear Chagas Disease Colombia Diagnostic Accuracy Diagnostic Test Accuracy Study Discrete Typing Unit Disease Course Enzyme Linked Immunosorbent Assay Genetic Variability Genotype Hemagglutination Inhibition Test Human Immunofluorescence Test Male Microorganism Detection Molecular Diagnosis Nonhuman Parasite Load Parasitemia Predictive Value Real Time Polymerase Chain Reaction Receiver Operating Characteristic Sensitivity And Specificity Trypanosoma Cruzi Acute Disease Chagas Disease Chronic Disease Comparative Study Exon Genetic Variation Isolation And Purification Molecular Typing Parasite Load Young Adult Acute Disease Chagas Disease Chronic Disease Colombia Dna, Protozoan Exons Genetic Variation Genotype Male Molecular Typing Parasite Load Real-Time Polymerase Chain Reaction Sensitivity And Specificity Trypanosoma Cruzi Young Adult
topic	Molecular Marker Protozoal Dna Spacer Dna Area Under The Curve Blood Culture Blood Smear Chagas Disease Colombia Diagnostic Accuracy Diagnostic Test Accuracy Study Discrete Typing Unit Disease Course Enzyme Linked Immunosorbent Assay Genetic Variability Genotype Hemagglutination Inhibition Test Human Immunofluorescence Test Male Microorganism Detection Molecular Diagnosis Nonhuman Parasite Load Parasitemia Predictive Value Real Time Polymerase Chain Reaction Receiver Operating Characteristic Sensitivity And Specificity Trypanosoma Cruzi Acute Disease Chagas Disease Chronic Disease Comparative Study Exon Genetic Variation Isolation And Purification Molecular Typing Parasite Load Young Adult Acute Disease Chagas Disease Chronic Disease Colombia Dna, Protozoan Exons Genetic Variation Genotype Male Molecular Typing Parasite Load Real-Time Polymerase Chain Reaction Sensitivity And Specificity Trypanosoma Cruzi Young Adult Enfermedades Adult Article Female Middle Aged Adult Female Humans Middle Aged Enfermedad de chagas Parasitemia Trypanosoma cruzi
dc.subject.ddc.spa.fl_str_mv	Enfermedades
dc.subject.keyword.spa.fl_str_mv	Adult Article Female Middle Aged Adult Female Humans Middle Aged
dc.subject.lemb.spa.fl_str_mv	Enfermedad de chagas Parasitemia Trypanosoma cruzi
description	Fe de erratas. The fifth author's name is spelled incorrectly. The correct name is: Carlos A. Valencia-Hernandez. The correct citation is: Hernández C, Cucunubá Z, Flórez C, Olivera M, Valencia-Hernandez C.A., Zambrano P, et al. (2016) Molecular Diagnosis of Chagas Disease in Colombia: Parasitic Loads and Discrete Typing Units in Patients from Acute and Chronic Phases. PLoS Negl Trop Dis 10(9): e0004997. doi: 10.1371/journal.pntd.0004997
publishDate	2016
dc.date.created.none.fl_str_mv	2016-10-28
dc.date.issued.none.fl_str_mv	2016
dc.date.accessioned.none.fl_str_mv	2019-01-22T20:49:54Z
dc.date.available.none.fl_str_mv	2019-01-22T20:49:54Z
dc.type.eng.fl_str_mv	article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv	http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv	Artículo
dc.identifier.issn.none.fl_str_mv	Issn 1935-2727
dc.identifier.uri.none.fl_str_mv	http://repository.urosario.edu.co/handle/10336/18908
identifier_str_mv	Issn 1935-2727
url	http://repository.urosario.edu.co/handle/10336/18908
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.citationIssue.none.fl_str_mv	No. 9
dc.relation.citationTitle.none.fl_str_mv	PLoS Neglected Tropical Diseases
dc.relation.citationVolume.none.fl_str_mv	Vol. 10
dc.relation.ispartof.spa.fl_str_mv	PLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 10/No. 9 (2016)
dc.relation.uri.spa.fl_str_mv	https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005112&type=printable http://repository.urosario.edu.co/handle/10336/18907
dc.rights.coar.fl_str_mv	http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv	Abierto (Texto Completo)
dc.rights.cc.spa.fl_str_mv	https://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv	Abierto (Texto Completo) https://creativecommons.org/licenses/by/4.0/ http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv	application/pdf
institution	Universidad del Rosario
dc.source.bibliographicCitation.spa.fl_str_mv	http://www.who.int/wer/2015/wer9006.pdf, WHO. Weekly epidemiological record—Chagas diseases in Latin America: an epidemiological update based on 2010 estimates. 2015 p. 5–13. Available:
dc.source.instname.none.fl_str_mv	instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv	reponame:Repositorio Institucional EdocUR
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repository.name.fl_str_mv	Repositorio institucional EdocUR
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spelling	6fac5401-cfd3-4a47-bd30-267447b53799600180780cc-4c03-4eef-bdea-5f111ebe20ec6001ba4f510-30ed-403d-b3df-d6d598932a1d6006a4f0aa3-2829-41e8-9c65-4d970a1be9706007ee13275-53d2-4d0e-b53f-da124ee4b64b6003fba508f-342c-451c-a0e7-f3f2036f6b766000fabd227-d409-4ad3-9615-bdbcb7e0ad7b60010117161186002019-01-22T20:49:54Z2019-01-22T20:49:54Z2016-10-282016Fe de erratas. The fifth author's name is spelled incorrectly. The correct name is: Carlos A. Valencia-Hernandez. The correct citation is: Hernández C, Cucunubá Z, Flórez C, Olivera M, Valencia-Hernandez C.A., Zambrano P, et al. (2016) Molecular Diagnosis of Chagas Disease in Colombia: Parasitic Loads and Discrete Typing Units in Patients from Acute and Chronic Phases. PLoS Negl Trop Dis 10(9): e0004997. doi: 10.1371/journal.pntd.0004997Background: The diagnosis of Chagas disease is complex due to the dynamics of parasitemia in the clinical phases of the disease. The molecular tests have been considered promissory because they detect the parasite in all clinical phases. Trypanosoma cruzi presents significant genetic variability and is classified into six Discrete Typing Units TcI-TcVI (DTUs) with the emergence of foreseen genotypes within TcI as TcIDom and TcI Sylvatic. The objective of this study was to determine the operating characteristics of molecular tests (conventional and Real Time PCR) for the detection of T. cruzi DNA, parasitic loads and DTUs in a large cohort of Colombian patients from acute and chronic phases. Methodology/Principal Findings: Samples were obtained from 708 patients in all clinical phases. Standard diagnosis (direct and serological tests) and molecular tests (conventional PCR and quantitative PCR) targeting the nuclear satellite DNA region. The genotyping was performed by PCR using the intergenic region of the mini-exon gene, the 24Sa, 18S and A10 regions. The operating capabilities showed that performance of qPCR was higher compared to cPCR. Likewise, the performance of qPCR was significantly higher in acute phase compared with chronic phase. The median parasitic loads detected were 4.69 and 1.33 parasite equivalents/mL for acute and chronic phases. The main DTU identified was TcI (74.2%). TcIDom genotype was significantly more frequent in chronic phase compared to acute phase (82.1% vs 16.6%). The median parasitic load for TcIDom was significantly higher compared with TcI Sylvatic in chronic phase (2.58 vs.0.75 parasite equivalents/ml). Conclusions/Significance: The molecular tests are a precise tool to complement the standard diagnosis of Chagas disease, specifically in acute phase showing high discriminative power. However, it is necessary to improve the sensitivity of molecular tests in chronic phase. The frequency and parasitemia of TcIDom genotype in chronic patients highlight its possible relationship to the chronicity of the disease. © 2016 Hernández et al.application/pdfIssn 1935-2727http://repository.urosario.edu.co/handle/10336/18908engNo. 9PLoS Neglected Tropical DiseasesVol. 10PLoS Neglected Tropical Diseases, ISSN: 1935-2727, Vol. 10/No. 9 (2016)https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0005112&type=printablehttp://repository.urosario.edu.co/handle/10336/18907Abierto (Texto Completo)https://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2http://www.who.int/wer/2015/wer9006.pdf, WHO. Weekly epidemiological record—Chagas diseases in Latin America: an epidemiological update based on 2010 estimates. 2015 p. 5–13. Available:instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURMolecular MarkerProtozoal DnaSpacer DnaArea Under The CurveBlood CultureBlood SmearChagas DiseaseColombiaDiagnostic AccuracyDiagnostic Test Accuracy StudyDiscrete Typing UnitDisease CourseEnzyme Linked Immunosorbent AssayGenetic VariabilityGenotypeHemagglutination Inhibition TestHumanImmunofluorescence TestMaleMicroorganism DetectionMolecular DiagnosisNonhumanParasite LoadParasitemiaPredictive ValueReal Time Polymerase Chain ReactionReceiver Operating CharacteristicSensitivity And SpecificityTrypanosoma CruziAcute DiseaseChagas DiseaseChronic DiseaseComparative StudyExonGenetic VariationIsolation And PurificationMolecular TypingParasite LoadYoung AdultAcute DiseaseChagas DiseaseChronic DiseaseColombiaDna, ProtozoanExonsGenetic VariationGenotypeMaleMolecular TypingParasite LoadReal-Time Polymerase Chain ReactionSensitivity And SpecificityTrypanosoma CruziYoung AdultEnfermedades616600AdultArticleFemaleMiddle AgedAdultFemaleHumansMiddle AgedEnfermedad de chagasParasitemiaTrypanosoma cruziCorrection : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phasesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Hernández, CarolinaCucunubá, ZulmaFlórez, CarolinaOlivera, MarioValencia-Hernandez, Carlos A.Zambrano, PilarLeón, CieloRamírez, Juan DavidHernández, CarolinaCucunubá, ZulmaFlórez, CarolinaOlivera, MarioValencia-Hernandez, Carlos A.Zambrano, PilarLeón, CieloRamírez, Juan DavidORIGINAL59.pdfapplication/pdf350338https://repository.urosario.edu.co/bitstreams/be1062e9-eff5-4a25-96d2-0f3e525b67d5/download985eb39ffc2d36d07c0b4ca921365c79MD51TEXT59.pdf.txt59.pdf.txtExtracted texttext/plain1812https://repository.urosario.edu.co/bitstreams/4ed085df-eb08-4f82-9432-bf52d1122f6f/downloadf39ad387dcf2b126782509ee9f7e7122MD52THUMBNAIL59.pdf.jpg59.pdf.jpgGenerated Thumbnailimage/jpeg3432https://repository.urosario.edu.co/bitstreams/edfc12ef-ab30-4774-8a5f-6d5d5b5b5c61/download51f99a3238b8e2dfa1a077330cfa8632MD5310336/18908oai:repository.urosario.edu.co:10336/189082019-09-19 07:37:54.609585https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co

Correction : Molecular diagnosis of Chagas disease in Colombia : Parasitic loads and discrete typing units in patients from acute and chronic phases

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