Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo

Las Actinobacterias de ambientes tropicales no intervenidos provenientes de los ecosistemas de páramo de Colombia, pueden ser novedosos en sus adaptaciones microbianas por la presencia de BGCs (clústers de genes biosintéticos), genes de resistencia y moléculas bioactivas. En este estudio se caracter...

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Fecha de publicación:
2022
Institución:
Universidad del Rosario
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Repositorio EdocUR - U. Rosario
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spa
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Acceso en línea:
https://doi.org/10.48713/10336_34793
https://repository.urosario.edu.co/handle/10336/34793
Palabra clave:
Actinobacterias de ambientes tropicales
Actividad antimicrobiana
Ecosistemas de páramo de Colombia
Adaptaciones microbianas
Análisis microbianos
Antibacteriales
Microbiología
Actinobacteria from tropical environments
Antimicrobial activity
Paramo Ecosystems of Colombia
Microbial adaptations
Microbial analysis
Antibacterial
Rights
License
Restringido (Temporalmente bloqueado)
id EDOCUR2_9c2735165b4ef767cb0f06083f42af18
oai_identifier_str oai:repository.urosario.edu.co:10336/34793
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
dc.title.es.fl_str_mv Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
dc.title.TranslatedTitle.es.fl_str_mv Genomics and in vitro characterization of actinobacteria from tropical environments reveals their bioactive potential
title Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
spellingShingle Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
Actinobacterias de ambientes tropicales
Actividad antimicrobiana
Ecosistemas de páramo de Colombia
Adaptaciones microbianas
Análisis microbianos
Antibacteriales
Microbiología
Actinobacteria from tropical environments
Antimicrobial activity
Paramo Ecosystems of Colombia
Microbial adaptations
Microbial analysis
Antibacterial
title_short Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
title_full Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
title_fullStr Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
title_full_unstemmed Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
title_sort Genómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivo
dc.contributor.advisor.none.fl_str_mv Zambrano, Maria Mercedes
dc.contributor.none.fl_str_mv Corrales, Adriana
dc.subject.es.fl_str_mv Actinobacterias de ambientes tropicales
Actividad antimicrobiana
Ecosistemas de páramo de Colombia
Adaptaciones microbianas
Análisis microbianos
Antibacteriales
topic Actinobacterias de ambientes tropicales
Actividad antimicrobiana
Ecosistemas de páramo de Colombia
Adaptaciones microbianas
Análisis microbianos
Antibacteriales
Microbiología
Actinobacteria from tropical environments
Antimicrobial activity
Paramo Ecosystems of Colombia
Microbial adaptations
Microbial analysis
Antibacterial
dc.subject.ddc.es.fl_str_mv Microbiología
dc.subject.keyword.es.fl_str_mv Actinobacteria from tropical environments
Antimicrobial activity
Paramo Ecosystems of Colombia
Microbial adaptations
Microbial analysis
Antibacterial
description Las Actinobacterias de ambientes tropicales no intervenidos provenientes de los ecosistemas de páramo de Colombia, pueden ser novedosos en sus adaptaciones microbianas por la presencia de BGCs (clústers de genes biosintéticos), genes de resistencia y moléculas bioactivas. En este estudio se caracterizó el potencial funcional de siete Actinobacterias provenientes del Parque Nacional Natural (PNN) de los Nevados y PNN Chingaza en condiciones de laboratorio y a nivel genómico. 16 aislamientos fueron evaluados mediante pruebas de actividad antimicrobiana contra bacterias de importancia clínica como Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae y Pseudomonas aeruginosa. De estos, se seleccionaron siete del fílum Actinobacteria según clasificación por el gen 16S rRNA. Algunos de los sobrenadantes de estas Actinobacterias, pertenecientes a los géneros Arthrobacter sp., Streptomyces , Subtercola sp, Amycolatopsis sp. y Rhodococccus sp., inhibieron el crecimiento de E. coli en un 47%, K. pneumoniae en un 68%, S. aureus 15% y P. aeuruginosa 28%. Se secuenciaron siete genomas mediante MinION (NanoPore) y cinco mediante Illumina (Novaseq 6000). Tras ensamblaje, de novo de secuencias largas (ONT) e híbridos (ONT+Illumina), más anotación funcional, se encontraron BGCs de diversas rutas biosintéticas como terpenos, RiPP-like, NRPS, PKS, ectoina, sideróforos y oligosacáridos. Estos BGCs se asociaron a 33 posibles compuestos bioactivos con funciones antibióticas, antioxidantes, antitumorales, quelantes y de osmorregulación. Se encontraron 2557 genes de resistencia asociados a mecanismos de resistencia por expulsión del antibiótico, alteración y reemplazo del diana del antibiótico, inactivación del antibiótico y reducción de la permeabilidad del antibiótico. Tanto la actividad antimicrobiana in vitro de estos aislamientos, como la presencia de BGCs y de genes de resistencia a antibióticos indican diversidad genómica y capacidad funcional asociada a producción de metabolitos bioactivos en Actinobacterias de páramo. Este trabajo sienta las bases para caracterizar nuevos compuestos antimicrobianos y potencial funcional en microorganismos ambientales.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-08-25T19:34:13Z
dc.date.available.none.fl_str_mv 2022-08-25T19:34:13Z
dc.date.created.none.fl_str_mv 2022-08-23
dc.date.embargoEnd.none.fl_str_mv info:eu-repo/date/embargoEnd/2023-08-25
dc.type.es.fl_str_mv bachelorThesis
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.document.es.fl_str_mv Trabajo de grado
dc.type.spa.es.fl_str_mv Trabajo de grado
dc.identifier.doi.none.fl_str_mv https://doi.org/10.48713/10336_34793
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/34793
url https://doi.org/10.48713/10336_34793
https://repository.urosario.edu.co/handle/10336/34793
dc.language.iso.es.fl_str_mv spa
language spa
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_f1cf
dc.rights.acceso.es.fl_str_mv Restringido (Temporalmente bloqueado)
rights_invalid_str_mv Restringido (Temporalmente bloqueado)
http://purl.org/coar/access_right/c_f1cf
dc.format.extent.es.fl_str_mv 68 pp
dc.format.mimetype.es.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad del Rosario
dc.publisher.department.none.fl_str_mv Facultad de Ciencias Naturales
dc.publisher.program.none.fl_str_mv Maestría en Ciencias Naturales
publisher.none.fl_str_mv Universidad del Rosario
institution Universidad del Rosario
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spelling Corrales, Adriana43260206600Zambrano, Maria Mercedes0b689b0d-1235-4ba1-a2ac-67bd0b5142d9600Vargas Florez, María NathaliaMagíster en Ciencias NaturalesMaestríaFull timeb43cebfa-aeca-43a5-b766-45da1743d9d86002022-08-25T19:34:13Z2022-08-25T19:34:13Z2022-08-23info:eu-repo/date/embargoEnd/2023-08-25Las Actinobacterias de ambientes tropicales no intervenidos provenientes de los ecosistemas de páramo de Colombia, pueden ser novedosos en sus adaptaciones microbianas por la presencia de BGCs (clústers de genes biosintéticos), genes de resistencia y moléculas bioactivas. En este estudio se caracterizó el potencial funcional de siete Actinobacterias provenientes del Parque Nacional Natural (PNN) de los Nevados y PNN Chingaza en condiciones de laboratorio y a nivel genómico. 16 aislamientos fueron evaluados mediante pruebas de actividad antimicrobiana contra bacterias de importancia clínica como Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae y Pseudomonas aeruginosa. De estos, se seleccionaron siete del fílum Actinobacteria según clasificación por el gen 16S rRNA. Algunos de los sobrenadantes de estas Actinobacterias, pertenecientes a los géneros Arthrobacter sp., Streptomyces , Subtercola sp, Amycolatopsis sp. y Rhodococccus sp., inhibieron el crecimiento de E. coli en un 47%, K. pneumoniae en un 68%, S. aureus 15% y P. aeuruginosa 28%. Se secuenciaron siete genomas mediante MinION (NanoPore) y cinco mediante Illumina (Novaseq 6000). Tras ensamblaje, de novo de secuencias largas (ONT) e híbridos (ONT+Illumina), más anotación funcional, se encontraron BGCs de diversas rutas biosintéticas como terpenos, RiPP-like, NRPS, PKS, ectoina, sideróforos y oligosacáridos. Estos BGCs se asociaron a 33 posibles compuestos bioactivos con funciones antibióticas, antioxidantes, antitumorales, quelantes y de osmorregulación. Se encontraron 2557 genes de resistencia asociados a mecanismos de resistencia por expulsión del antibiótico, alteración y reemplazo del diana del antibiótico, inactivación del antibiótico y reducción de la permeabilidad del antibiótico. Tanto la actividad antimicrobiana in vitro de estos aislamientos, como la presencia de BGCs y de genes de resistencia a antibióticos indican diversidad genómica y capacidad funcional asociada a producción de metabolitos bioactivos en Actinobacterias de páramo. Este trabajo sienta las bases para caracterizar nuevos compuestos antimicrobianos y potencial funcional en microorganismos ambientales.Actinobacteria from pristine tropical environments such as the Colombian paramos could have novel adaptations in biosynthetic gene clusters (BGCs), resistance genes and bioactive compounds. In this study, the functional potential of seven Actinobacteria from the National Natural Park (NNP) of Nevados and the NNP of Chingaza was characterized under in vitro laboratory conditions and the genomic level. 16 isolates from these environments were tested for antimicrobial activity against clinically important bacteria such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. Seven out of these 16 isolates were confirmed as Actinobacteria through 16S rRNA gene sequencing. The supernatants from some of these Actinobacterial isolates, such as Arthrobacter, Streptomyces, Subtercola, Amycolatopsis and Rhodococccus inhibited the growth of E. coli by 47%, K. pneumoniae by 68%, S. aureus by 15% and P. aeruginosa by 28%. The genomes of these seven Actinobacteria were sequenced through Nanopore (MinION) and five of these genomes were also sequenced through Illumina (Novaseq 6000). After long-read de novo assembly (for Nanopore) and hybrid assemblies (for Nanopore and Illumina), we found BGCs coding for various biosynthetic pathways such as terpenes, Ribosomally synthesized and post-translationally modified peptides (RiPP-like) products, Nonribosomal peptides (NRPS), Polyketide synthases (PKS), ectoine, siderophores and oligosaccharides. We found coding potential for 33 bioactive compounds with antibiotic, antioxidant, antitumor, chelating and osmoregulatory functions. Moreover, we found 2557 resistance genes associated with antibiotic expulsion, antibiotic target alteration and replacement, antibiotic inactivation, and reduced antibiotic permeability. The in vitro antimicrobial activity of these isolates, along with the presence of BGCs and antibiotic resistance genes, shows the genomic diversity and functional capacity associated with the production of bioactive metabolites by Actinobacteria from the paramos. This work lays the groundwork to characterize new antimicrobial compounds and functional potential in environmental microorganisms.2022-08-25 14:50:01: Script de automatizacion de embargos. Cordial saludo mi nombre es María Nathalia Vargas Flórez y acabo de subir al repositorio mi trabajo de grado. En este correo, Yo María Nathalia con C.C 1073517210, declaro que el documento titulado Genómica de Actinobacterias revela su potencial bioactivo tiene acceso restringido debido a que en los próximos meses de realizará la publicación de los datos obtenidos en la tesis. Gracias por su atenciónCorpoGenUniversidad del RosarioMinciencias68 ppapplication/pdfhttps://doi.org/10.48713/10336_34793 https://repository.urosario.edu.co/handle/10336/34793spaUniversidad del RosarioFacultad de Ciencias NaturalesMaestría en Ciencias NaturalesRestringido (Temporalmente bloqueado)http://purl.org/coar/access_right/c_f1cf1. Alcock B, Raphenya A, Lau T, Tsang K, Bouchard M, Edalatmand A, et al., (2020). 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Tetrahedron. 70: 7795-7801.instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURActinobacterias de ambientes tropicalesActividad antimicrobianaEcosistemas de páramo de ColombiaAdaptaciones microbianasAnálisis microbianosAntibacterialesMicrobiología576600Actinobacteria from tropical environmentsAntimicrobial activityParamo Ecosystems of ColombiaMicrobial adaptationsMicrobial analysisAntibacterialGenómica y caracterización in vitro de actinobacterias de ambientes tropicales revela su potencial bioactivoGenomics and in vitro characterization of actinobacteria from tropical environments reveals their bioactive potentialbachelorThesisTrabajo de gradoTrabajo de gradohttp://purl.org/coar/resource_type/c_7a1fLICENSElicense.txtlicense.txttext/plain1475https://repository.urosario.edu.co/bitstreams/9494950c-a52b-4b12-9340-88a328e7d709/downloadfab9d9ed61d64f6ac005dee3306ae77eMD52ORIGINALVargasFlorez_MariaNathalia_2022.pdfVargasFlorez_MariaNathalia_2022.pdfTesis de maestriaapplication/pdf2549308https://repository.urosario.edu.co/bitstreams/b25e963a-8c30-499c-87ec-c42e70ef255c/download4bbc2bea90ac65e4b63a3ac87136e3d9MD51TEXTVargasFlorez_MariaNathalia_2022.pdf.txtVargasFlorez_MariaNathalia_2022.pdf.txtExtracted texttext/plain102403https://repository.urosario.edu.co/bitstreams/3c5ef4bd-db58-4fdb-80df-2ec0bd6b5245/download3485e6bda1ce453c6d7946e3f40de47bMD53THUMBNAILVargasFlorez_MariaNathalia_2022.pdf.jpgVargasFlorez_MariaNathalia_2022.pdf.jpgGenerated Thumbnailimage/jpeg2480https://repository.urosario.edu.co/bitstreams/fb5c3b35-7385-45e8-aa97-016a1c9ffc6b/downloadaa27e2134bb361db3828ddca61aa2938MD5410336/34793oai:repository.urosario.edu.co:10336/347932022-08-30 14:40:02.042068https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.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