Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC

Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high bin...

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Autores:
Tipo de recurso:
Fecha de publicación:
2008
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/27002
Acceso en línea:
https://doi.org/10.1016/j.vaccine.2007.11.086
https://repository.urosario.edu.co/handle/10336/27002
Palabra clave:
P. falciparum
RAMA
Rhoptry
HABP
Rights
License
Restringido (Acceso a grupos específicos)
id EDOCUR2_9ade54a84f29c2d5ad43473a1a590b43
oai_identifier_str oai:repository.urosario.edu.co:10336/27002
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling c45f5c54-3321-4073-9258-76a2fd84e3b7-191225589-184cece1e-359c-45a6-b251-f8e4b62a84eb-151721018-1ea37e0fc-df77-4ae1-b1d3-aaf2304a69cd-110ecd4f9-843f-4ef2-bec0-7d39d3381a13-1518908816002020-08-19T14:40:43Z2020-08-19T14:40:43Z2008-02-06Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparum in vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis.application/pdfhttps://doi.org/10.1016/j.vaccine.2007.11.086ISSN: 0264-410XEISSN: 1873-2518https://repository.urosario.edu.co/handle/10336/27002engThe Japanese Society for VaccinologyElsevier862No. 6853VaccineVol. 26Vaccine, ISSN: 0264-410X;EISSN: 1873-2518, Vol.26, No.6 (2008); pp. 853-862 https://www.sciencedirect.com/science/article/pii/S0264410X07013072?via%3DihubRestringido (Acceso a grupos específicos)http://purl.org/coar/access_right/c_16ecVaccineinstname:Universidad del Rosarioreponame:Repositorio Institucional EdocURP. falciparumRAMARhoptryHABPStudies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBCEstudios de péptidos de la proteína del antígeno de membrana asociado a rhoptry (RAMA) de Plasmodium falciparum que se unen específicamente a los glóbulos rojos humanosarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Pinzón, Carlos GiovanniCurtidor, HernandoForero, MarthaVanegas, MagnoliaRodríguez, JorgePatarroyo, Manuel E.Bermudez, Adriana10336/27002oai:repository.urosario.edu.co:10336/270022021-06-03 00:50:03.96https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
dc.title.TranslatedTitle.spa.fl_str_mv Estudios de péptidos de la proteína del antígeno de membrana asociado a rhoptry (RAMA) de Plasmodium falciparum que se unen específicamente a los glóbulos rojos humanos
title Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
spellingShingle Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
P. falciparum
RAMA
Rhoptry
HABP
title_short Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
title_full Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
title_fullStr Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
title_full_unstemmed Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
title_sort Studies of plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC
dc.subject.keyword.spa.fl_str_mv P. falciparum
RAMA
Rhoptry
HABP
topic P. falciparum
RAMA
Rhoptry
HABP
description Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparum in vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis.
publishDate 2008
dc.date.created.spa.fl_str_mv 2008-02-06
dc.date.accessioned.none.fl_str_mv 2020-08-19T14:40:43Z
dc.date.available.none.fl_str_mv 2020-08-19T14:40:43Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.vaccine.2007.11.086
dc.identifier.issn.none.fl_str_mv ISSN: 0264-410X
EISSN: 1873-2518
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/27002
url https://doi.org/10.1016/j.vaccine.2007.11.086
https://repository.urosario.edu.co/handle/10336/27002
identifier_str_mv ISSN: 0264-410X
EISSN: 1873-2518
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationEndPage.none.fl_str_mv 862
dc.relation.citationIssue.none.fl_str_mv No. 6
dc.relation.citationStartPage.none.fl_str_mv 853
dc.relation.citationTitle.none.fl_str_mv Vaccine
dc.relation.citationVolume.none.fl_str_mv Vol. 26
dc.relation.ispartof.spa.fl_str_mv Vaccine, ISSN: 0264-410X;EISSN: 1873-2518, Vol.26, No.6 (2008); pp. 853-862
dc.relation.uri.spa.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0264410X07013072?via%3Dihub
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_16ec
dc.rights.acceso.spa.fl_str_mv Restringido (Acceso a grupos específicos)
rights_invalid_str_mv Restringido (Acceso a grupos específicos)
http://purl.org/coar/access_right/c_16ec
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv The Japanese Society for Vaccinology
Elsevier
dc.source.spa.fl_str_mv Vaccine
institution Universidad del Rosario
dc.source.instname.none.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.none.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
_version_ 1814167437257474048

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