Sarconesiopsis magellanica (Diptera: Calliphoridae) excretions and secretions have potent antibacterial activity

The most important mechanism for combating infection using larval therapy depends on larval excretions and secretions (ES). The present work was aimed at evaluating Sarconesiopsis magellanica (Diptera: Calliphoridae) ES antibacterial activity in six bacterial strains (three Gram-positive and three Gram-negative) and comparing this to the effect of Lucilia sericata-derived ES. Antibacterial activity at 50. ?g/mL minimum inhibitory concentration (MIC) was observed for Staphylococcus epidermidis ATCC-12228 and Staphylococcus aureus ATCC-29213 strains, when the turbidimetry test involving S. magellanica ES was used; the rest of the bacterial strains (Staphylococcus aureus ATCC-6538, Pseudomonas aeruginosa ATCC-10145, Pseudomonas aeruginosa ATCC-9027 and Pseudomonas aeruginosa ATCC-27853) were inhibited at a 100. ?g/mL MIC. Twice the amount was required to inhibit the aforementioned bacteria with L. sericata-derived ES using this same technique; a similar trend was observed when the agar diffusion method was used instead. Furthermore, when the previously established MIC for each bacterial strain was used, their colonies became reduced following 1-6. h incubation with S. magellanica derived ES, whilst the reduction occurred from 2 to 6. hours with those from L. sericata. Although the MIC for each strain obtained with ciprofloxacin was lower than those established when using either blowfly derived-ES, the gradual reduction of the colonies occurred at a longer incubation time (6. h or more). The results showed that S. magellanica ES antibacterial activity was more potent and effective, compared to that of L. sericata-derived ES. © 2014 Elsevier B.V.

• Fecha de publicación: 2014
• Institución: Universidad del Rosario
• Repositorio: Repositorio EdocUR - U. Rosario
• Idioma: eng
• OAI Identifier: oai:repository.urosario.edu.co:10336/23597
• Acceso en línea: https://doi.org/10.1016/j.actatropica.2014.04.018; https://repository.urosario.edu.co/handle/10336/23597
• Palabra clave: Natural product; Antiinfective agent; Insect protein; Antimicrobial activity; Comparative study; Excretion; Incubation; Inhibitor; Insect; Secretion; Turbidity; Agar diffusion; Antibacterial activity; Article; Bacterial strain; Biological activity; Bodily secretions; Calliphoridae; Colony formation; Controlled study; Drug potency; Excretion; Gram negative bacterium; Gram positive bacterium; In vitro study; Incubation time; Larval stage; Lucilia sericata; Minimum inhibitory concentration; Nonhuman; Pseudomonas aeruginosa; Sarconesiopsis magellanica; Species comparison; Staphylococcus aureus; Staphylococcus epidermidis; Animal; Bodily secretions; Chemistry; Diptera; Drug effects; Metabolism; Microbial sensitivity test; Staphylococcus; Animals; Anti-bacterial agents; Bodily secretions; Diptera; Insect proteins; Microbial sensitivity tests; Pseudomonas aeruginosa; Staphylococcus; Antibacterial activity; Excretions and secretions; Larval therapy; Minimum inhibitory concentration; Sarconesiopsis magellanica
• License: Abierto (Texto Completo)