Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes

Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we obs...

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Tipo de recurso:
Fecha de publicación:
2019
Institución:
Universidad del Rosario
Repositorio:
Repositorio EdocUR - U. Rosario
Idioma:
eng
OAI Identifier:
oai:repository.urosario.edu.co:10336/23286
Acceso en línea:
https://doi.org/10.1038/s41598-019-45228-6
https://repository.urosario.edu.co/handle/10336/23286
Palabra clave:
Complement
Receptor1
availability
red
blood
cell
surface
modulates
Plasmodium
vivax
invasion
human
reticulocytes
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License
Abierto (Texto Completo)
id EDOCUR2_95b3e24988d6902075fbe6e17b45511b
oai_identifier_str oai:repository.urosario.edu.co:10336/23286
network_acronym_str EDOCUR2
network_name_str Repositorio EdocUR - U. Rosario
repository_id_str
spelling 8208466c-c023-4a2d-95d3-f2d4a0b610cc-195a6eb5e-8a35-4d6d-90bd-9a26ae6869b7-1a6c040ce-dc6e-43bd-9cff-654f1f8e2ea5-1f86a4770-1da0-459d-89cf-66701309c49d-1c0fde662-e86f-452f-a95a-af51d17b6962-1591617a9-a0d1-4b0a-a83c-e3e64358bbf0-16b7bf68c-0e03-457f-a06b-034855476069-15ecc8d2b-f41d-4972-90e8-7c1d82496c9e-1af6a397a-4337-4175-96cc-b8608de40e9f-1f09d40ad-fa49-4e48-bb5f-a8744baa41e1-1feb05106-a473-4c68-8310-9a9a4551b310-1fa8b927c-b57f-4fdf-9873-deb7d8cf5c38-156ed6fce-59b3-494a-813f-840c3564c0aa-1e75126fa-9db9-4061-81f6-bfd49db375da-116b1b611-a09b-4c25-83a4-22cf1af90d3f-179653065-131eb8eae-d939-430a-8b1c-24ca9b81208f-118d5dfdf-ab71-4ff1-92f9-8195b160e8aa-1df545bca-ad84-4e03-9cb2-714eba0599d2-11e81e5d9-43cd-477f-bb6f-33c36fa377d1-12020-05-26T00:00:55Z2020-05-26T00:00:55Z2019Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria. © 2019, The Author(s).application/pdfhttps://doi.org/10.1038/s41598-019-45228-620452322https://repository.urosario.edu.co/handle/10336/23286engNature Publishing GroupNo. 1Scientific ReportsVol. 9Scientific Reports, ISSN:20452322, Vol.9, No.1 (2019)https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067645951&doi=10.1038%2fs41598-019-45228-6&partnerID=40&md5=5bb95dcb167113fbde0687ee775e5d84Abierto (Texto Completo)http://purl.org/coar/access_right/c_abf2instname:Universidad del Rosarioreponame:Repositorio Institucional EdocURComplementReceptor1availabilityredbloodcellsurfacemodulatesPlasmodiumvivaxinvasionhumanreticulocytesComplement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytesarticleArtículohttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501Prajapati, Surendra KumarBorlon, CélineRovira-Vallbona, EduardGruszczyk, JakubMenant, SebastienTham, Wai-HongKattenberg, Johanna HelenaVillasis, ElizabethDe Meulenaere, KatlijnGamboa, DioniciaVinetz, JosephFujita, RicardoXuan, Xa NguyenUrbano Ferreira, MarceloNiño, Carlos H.Patarroyo, Manuel A.Spanakos, GregoryKestens, LucAbbeele, Jan Van DenRosanas-Urgell, Anna10336/23286oai:repository.urosario.edu.co:10336/232862022-05-02 07:37:14.58476https://repository.urosario.edu.coRepositorio institucional EdocURedocur@urosario.edu.co
dc.title.spa.fl_str_mv Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
title Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
spellingShingle Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
Complement
Receptor1
availability
red
blood
cell
surface
modulates
Plasmodium
vivax
invasion
human
reticulocytes
title_short Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
title_full Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
title_fullStr Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
title_full_unstemmed Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
title_sort Complement Receptor 1 availability on red blood cell surface modulates Plasmodium vivax invasion of human reticulocytes
dc.subject.keyword.spa.fl_str_mv Complement
Receptor1
availability
red
blood
cell
surface
modulates
Plasmodium
vivax
invasion
human
reticulocytes
topic Complement
Receptor1
availability
red
blood
cell
surface
modulates
Plasmodium
vivax
invasion
human
reticulocytes
description Plasmodium vivax parasites preferentially invade reticulocyte cells in a multistep process that is still poorly understood. In this study, we used ex vivo invasion assays and population genetic analyses to investigate the involvement of complement receptor 1 (CR1) in P. vivax invasion. First, we observed that P. vivax invasion of reticulocytes was consistently reduced when CR1 surface expression was reduced through enzymatic cleavage, in the presence of naturally low-CR1-expressing cells compared with high-CR1-expressing cells, and with the addition of soluble CR1, a known inhibitor of P. falciparum invasion. Immuno-precipitation experiments with P. vivax Reticulocyte Binding Proteins showed no evidence of complex formation. In addition, analysis of CR1 genetic data for worldwide human populations with different exposure to malaria parasites show significantly higher frequency of CR1 alleles associated with low receptor expression on the surface of RBCs and higher linkage disequilibrium in human populations exposed to P. vivax malaria compared with unexposed populations. These results are consistent with a positive selection of low-CR1-expressing alleles in vivax-endemic areas. Collectively, our findings demonstrate that CR1 availability on the surface of RBCs modulates P. vivax invasion. The identification of new molecular interactions is crucial to guiding the rational development of new therapeutic interventions against vivax malaria. © 2019, The Author(s).
publishDate 2019
dc.date.created.spa.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2020-05-26T00:00:55Z
dc.date.available.none.fl_str_mv 2020-05-26T00:00:55Z
dc.type.eng.fl_str_mv article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.spa.spa.fl_str_mv Artículo
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1038/s41598-019-45228-6
dc.identifier.issn.none.fl_str_mv 20452322
dc.identifier.uri.none.fl_str_mv https://repository.urosario.edu.co/handle/10336/23286
url https://doi.org/10.1038/s41598-019-45228-6
https://repository.urosario.edu.co/handle/10336/23286
identifier_str_mv 20452322
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.citationIssue.none.fl_str_mv No. 1
dc.relation.citationTitle.none.fl_str_mv Scientific Reports
dc.relation.citationVolume.none.fl_str_mv Vol. 9
dc.relation.ispartof.spa.fl_str_mv Scientific Reports, ISSN:20452322, Vol.9, No.1 (2019)
dc.relation.uri.spa.fl_str_mv https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067645951&doi=10.1038%2fs41598-019-45228-6&partnerID=40&md5=5bb95dcb167113fbde0687ee775e5d84
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.acceso.spa.fl_str_mv Abierto (Texto Completo)
rights_invalid_str_mv Abierto (Texto Completo)
http://purl.org/coar/access_right/c_abf2
dc.format.mimetype.none.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Nature Publishing Group
institution Universidad del Rosario
dc.source.instname.spa.fl_str_mv instname:Universidad del Rosario
dc.source.reponame.spa.fl_str_mv reponame:Repositorio Institucional EdocUR
repository.name.fl_str_mv Repositorio institucional EdocUR
repository.mail.fl_str_mv edocur@urosario.edu.co
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